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Kasiyati, Menik
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Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Nursing Public Health Other

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Chymase Level in Dengue Virus Infection With or Without Positive Non-Structural 1(NS1) Kasiyati, Menik; Nugraha, Jusak; Kahar, Hartono
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1227.342 KB) | DOI: 10.29238/teknolabjournal.v8i2.167

Abstract

Thrombocytopenia, leukopenia, and monocytosis are laboratory parameters in the diagnosis of dengue virus infection. In addition to monocyte cells, mast cells also play a role in the innate immune response, where degranulation of mast cells will occur, which will secretion the active vaso mediator, Chymase. Chymase has a role in increasing vascular permeability resulting in plasma leakage in patients with dengue virus infection to determine the number of platelets, leukocytes, monocytes and chymase levels in patients with dengue infection in the acute phase. The platelet count mean in NS1 (+) was 132,140 cells / mm3 and the platelet count in the NS1 group was (-) 176,000 cells / mm3. The mean leukocytes NS1 (+) showed results of 4,350 cells / mm3 and NS1 (-) 5,250 cells / mm3. The mean monocyte NS1 (+) monocyte count was 8.26%, and NS1 (-) group was 8.76%. There were no significant differences in platelet counts, leukocytes and monocytes between NS1 (+) and NS1 (-) (P value> 0.05). The mean Chymase NS1 (+) 23.48, NS1 (-) 23.05 ng / mL and the control group 1.47ng / mL.
Perbedaan Hasil Pemeriksaan Tes Fungsi Ginjal pada Serum Pasien Tuberkulosis dengan dan Tanpa Pengenceran Kasiyati, Menik; Tri Widada, Subrata; Adnan, Muhammad; Nugerohojati, Darojatun; Fauzi, Afrizal Ahmad
Innovative: Journal Of Social Science Research Vol. 4 No. 6 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i6.13211

Abstract

Tuberkulosis adalah suatu penyakit menular yang disebabkan oleh bakteri Mycobacterium tuberculosis. Salah satu terapi yang diberikan kepada pasien tuberkulosis adalah pemberian OAT (Obat Anti Tuberkulosis). Pemeriksaan tes fungsi hati dianjurkan untuk dilakukan sebelum dan saat pemantauan pengobatan TBC. Peningkatan kreatinin, asam urat dan ureum merupakan beberapa krieria pasien tuberkulosis paru mengalami cedera ginjal akibat penggunaan OAT. Keadaan serum yang pekat ini dapat mempengaruhi kualitas hasil pemeriksaan laboratorium. Menurut Hukum Lambert Beer nilai absorbansi berbanding lurus dengan konsentrasi zat. Hal ini tidak berlaku pada larutan pekat, akibatnya kadar yang dihasilkan tidak akurat. Hasil yang tidak akurat dapat merugikan pasien karena menyebabkan kesalahan diagnosis, pengobatan dan pemantauan penyakit. Salah satu penanganan serum pekat adalah dengan perlakuan pengenceran. Pengencenan dilakukan dengan penambahan NaCL fisiologis perbandingan dengan harapan dapat mememperkecil tingkat kesalahan selama pengukuran sampel. Penelitian ini bertujuan untuk mengetahui perbedaan hasil tes fungsi ginjal pada serum pasien tuberkulosis dengan dan tanpa pengenceran. Penelitian ini merupakan penelitian Pre-experimental Design dengan desain penelitian One-Group Pretest-Posttest Design. Sampel  penelitian adalah serum pasien tuberkulosis dengan kadar kreatinin >1,3 mg/dL, kadar ureum >7 mg/dL dan kadar asam urat  >7 mg/dL. Hasil uji dianalisis deskriptif dan statistik menggunanakan uji beda Wilcoxon dengan taraf signifikan 5% untuk mengetahui ada tidaknya perbedaan hasil pemeriksaan. Hasil pemeriksaan tes fungsi ginjal yang diuji dengan uji beda Wilcoxon menunjukkan signifikan sebesar 0,000(<0,05) yang berarti ada perbedaan hasil pemeriksaan tes fungsi ginjal pada serum pasien tuberkulosis dengan dan tanpa pengenceran
CAMPURAN INFUSA SINGKONG (Manihot esculenta), KACANG KEDELAI (Glycine max (L.) Merrill) DAN EKSTRAK RAGI SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN BAKTERI Staphylococcus aureus Martsiningsih, Martha Atik; Astuti, Fardhiasih Dwi; Kasiyati, Menik; Suyana, Suyana; Rahmawati, Ullya; Rahayu, Muji; Aeni, Ajeng Puspa
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 7 No 1 (2024): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v7i1.19863

Abstract

The composition of nutrients needed by bacteria varies greatly such as peptone, meat extract, and agar. Nutrient Agar is one of the media used as a bacterial culture that has a composition such as protein and carbohydrates in the form of meat extract and peptone that suits the needs of most bacteria. The carbohydrate content in cassava, protein in soybeans, and nitrogen sources in yeast extract can be combined into alternative media used as bacterial growth media to determine whether the alternative media combination of cassava carbohydrate source infuse, soybean infuse, and yeast extract can be used to grow Staphylococcus aureus bacteria. This research is a pure experimental study with Post Test-Only Control Design research design. The mean growth in the number of colonies of Staphylococcus aureus bacteria on alternative media was 78,25 x  CFU/ml and NA media was 94.19 x   CFU/ml with a mean difference of 15.94 x  CFU/ml or 16.92%. The mean growth diameter of the number of colonies of Staphylococcus aureus bacteria on alternative media was 2.63 x  CFU/ml and NA media was 2.66 x   CFU/ml with a mean difference of 0.03 x  CFU/ml or 1.12%. The percentage of effectiveness of Staphylococcus aureus bacterial growth is 83.07% or moderately effective and the percentage of effectiveness of Staphylococcus aureus bacterial proliferation is 98.87% or effective. Alternative media mixture of cassava infuse (Manihot esculenta crantz), soybean (Glycine max (L.) Merrill) and yeast extract can be used to grow Staphylococcus aureus bacteria.
Analysis of NLR and MLR Values in Patients with Pulmonary Tuberculosis and Pulmonary Tuberculosis with Diabetes Mellitus Kuncara, Rachmad Bayu; Sasangka, Parisade Galih; Sugihantono, Anung; Duri, Iin Desmiany; Qomariyah, Nurul; Kasiyati, Menik; Azahra, Sresta; Sulistyasmi, Wiwit
Jurnal Kesehatan Vol 16 No 2 (2025): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v16i2.5149

Abstract

Tuberculosis (TB) infection can cause changes in hematological parameters such as neutrophils, monocytes, and lymphocytes. The neutrophil-to-lymphocyte ratio (NLR) and monocyte-to-lymphocyte ratio (MLR) are used as indicators of inflammation and immune response in infectious diseases, including TB. Diabetes mellitus (DM) as a comorbidity can exacerbate inflammation, potentially affecting NLR and MLR values in TB patients. This study aimed to determine the NLR and MLR values in patients with pulmonary TB and pulmonary TB with DM. This research used a quantitative observational design with a cross-sectional approach, involving 72 respondents from several primary health centers (Puskesmas) in Semarang City, divided into two groups of 36 individuals each. The sampling technique used was purposive sampling. The results showed that the mean NLR value in pulmonary TB patients was 2.20, and in pulmonary TB with DM patients was 2.89, with a significant difference (p=0.002). Meanwhile, the mean MLR value in pulmonary TB patients was 0.34 and in pulmonary TB with DM patients was 0.33, with no significant difference (p=0.752). These findings indicate that NLR is more sensitive than MLR in reflecting the inflammatory status and DM complications in TB patients. NLR can be used as an additional marker in the clinical monitoring of TB patients, especially those with DM comorbidity.
Gambaran Keton Urine Pada Penderita Diabetes Melitus Tipe 2 Kasiyati, Menik; Pudyastuti, Rita Rena; Ramadania, Arindha Desyifa; Rahayu, Muji
Innovative: Journal Of Social Science Research Vol. 4 No. 1 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i1.8459

Abstract

Latar Belakang : Diabetes Melitus adalah penyakit gangguan metabolik yang disebabkan oleh kegagalan pankreas untuk memproduksi hormon insulin dalam jumlah yang ideal Keton merupakan produk dari pemecahan asam lemak. Keberadaan keton dalam urine menandakan bahwa tubuh menggunakan lemak sebagai energi. Ketonuria merupakan kondisi dimana ditemukan adanya keton di dalam urine. Peningkatan ketonuria mengindikasikan tingginya keton dalam tubuh (ketoasidosis) yang dapat menyebabkan penurunan pH darah jika tidak segera mendapatkan penanganan dan mengarah pada ketoasidosis koma dan kematian. Tujuan : mengetahui gambaran keton urine pada penderita Diabetes Melitus Tipe 2 di Puskesmas Srandakan, Kabupaten Bantul. Metode : Penelitian ini adalah penelitian deskriptif dengan desain penelitian cross secsional. Penelitian ini dilaksanakan pada tanggal 20 Desember 2021 – 20 Januari 2022. Objek penelitian ini adalah pasien Diabetes Melitus Tipe 2 di Puskesmas Srandakan yang berpuasa minimal 8 jam sebelum melakukan pemeriksaan. Sampel berjumlah 120 pasien. Hasil : Pada 120 pasien Diabetes Melitus Tipe 2, didapatkan dari 97 pasien Diabetes Melitus Tipe 2 hiperglikemia sebanyak 4 pasien (3,3%) positif (+) keton, 10 pasien (8,3%) trace (±) keton dan 83 pasien (69,2%) negatif (-) keton. Pada pasien Diabetes Melitus Tipe 2 dengan kadar glukosa darah puasa normal yang berjumlah 22 pasien didapatkan 1 pasien (0,8%) trace keton dan 21 pasien (17,5%) negatif keton. Pada pasien Diabetes Melitus Tipe 2 hipoglikemia didapatkan 1 pasien (0,8%) dengan negatif keton. Kesimpulan : Gambaran keton urine pada 120 pasien Diabetes Melitus Tipe 2, dengan hasil 4 pasien (3,3%) dengan hasil pemeriksaan positif (+) keton, 11 pasien (9,2%) dengan hasil pemeriksaan trace (±) keton dan 105 pasien (87,5%) dengan hasil pemeriksaan negatif (-) keton.
Hasil Pewarnaan Sediaan Jaringan dengan Fiksatif Normal Buffer Formalin 10% dan Madu Selama Selama 1, 3, dan 7 Hari Nuryani, Siti; Kasiyati, Menik; Sujono, Sujono; Wikandari, Ririh Jatmi; Surati, Surati; Hardisari, Ratih
Innovative: Journal Of Social Science Research Vol. 4 No. 1 (2024): Innovative: Journal Of Social Science Research
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/innovative.v4i1.9148

Abstract

Pendahuluan: Pelayanan Patologi Anatomi merupakan pelayanan diagnostik dan laboratorium terhadap jaringan dan/atau cairan tubuh, di dalamnya terdapat teknik sitologi dan histologi yaitu fiksasi. Fiksasi selama ini menggunakan bahan formalin yang berbahaya, sehingga fiksatif lain seperti madu akan menjadi alternatif pilihan. Perlu kajian dan penelitian yang mendalam terkait madu sebagai bahan fiksatif sebelum digunakan dalam praktek sehari-hari di Laboratorium. Tujuan: Mengetahui pengaruh fiksatif madu terhadap hasil pewarnaan sediaan, pada konsentrasi 10, 15 dan 20% selama 1, 3 dan 7 hari. Metode: Objek adalah data primer yang didapat dari pengamatan gambaran mikroskopis sediaan jaringan yang dibuat dengan cara fiksasi menggunakan dua macam fiksatif yaitu Normal buffer formalin 10% sebagai control dan madu 10, 15, dan 20% dengan perendaman selama 1 , 3 dan 7 hari. Pewarnaan yang digunakan adalah Hematoksilin-Eosin. Binatang coba yang dipakai adalah tikus jantan dewasa, dan organ yang dikai adalah hepar, intertinal, limpa, ginjal dan testis. Data berupa skor gambaran histologis dianalisis secara diskriptif. Hasil : Organ lambung, Intestinal, Limpa, Ginjal, hepar dan testis menggunakan fiksatif kontrol NBF 10% masing-masing menunjukkan t skor 3. Madu 10% 1 hari skor rata-rata 1,5 ; dengan 15% 1 hari rata-sata skor 1,9: dengan 20% 1 hari rata-sata skor 1,3: dengan 20% 3 hari rata-sata skor 10.6 dan dengan 20% 7 hari rata-sata skor 0,4. Kesimpulan : Penggunaan madu 10, 15, dan 20% sebagai fiksatif dengan perendaman 1 hari, 3 hari dan 7 hari dari gambarab inti sel, sitoplasma dan kerusakan sel rata-rata menghasilkan skor dibawah NBF 10%.
Effect of The Temperature on The Size of Inhibition Zone of the Clindamycin, Levofloxacin, Tetracycline, and Trimethoprim Activity Against Staphylococcus aureus ATCC 25923 Sistiyono, Sistiyono; Martsiningsih, Marta Atik; Kasiyati, Menik; Rahayu, Muji; Pudyastuti, Rita Rena; Suyana, Suyana; Rahmawati, Ully; Rahmad; Faruq, Zulfikar Husni; Setiawan, Budi
JURNAL INFO KESEHATAN Vol 22 No 3 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss3.1353

Abstract

Antibiotic sensitivity testing is essential for determining bacterial susceptibility to antibiotics. In disc diffusion testing, several technical factors influence the diameter of the inhibition zone, including incubation temperature, which must be carefully controlled to ensure the validity of test results. This study aims to determine the mean, difference, and analyze the diameter of the inhibition zone of the antibiotics, namely Clindamycin, Levofloxacin, Tetracycline, and Trimethoprim against Staphylococcus aureus on Mueller-Hinton agar media with incubation temperatures of 33°C, 34°C, 35°C, 36°C and 37°C for 18 hours. This research is observational, with a cross-sectional design. The primary data is 100 data on the diameter of the antibiotic inhibition zone, obtained by measuring the diameter of the inhibition zone with different incubation temperatures. The selection of antibiotics is based on the mechanism of action of antibiotics inhibiting bacteria, namely the cell wall or cell membrane that surrounds the bacterial cell; the pieces of machinery that make the nucleic acids DNA and RNA, and the machinery that produces proteins (the ribosome and associated proteins) with a range of inhibition zones based on Internal Quality Control CLSI. The data and the repeated measure statistical test will be processed univariately to determine the significance of the difference in the diameter of the formed inhibition zone using the ANOVA test. The measurement of the inhibition zone diameter on the incubation temperature variation showed a significant difference with a p-value of 0.000 for Levofloxacin, Tetracycline, and Trimethoprim, while the p-value of Clindamycin is 0.010. For the other antibiotics, Levofloxacin, Tetracycline, and Trimethoprim antibiotics, the higher the incubation temperature, the average diameter of the inhibition zone is condensed, while for Clindamycin the higher the incubation temperature, the higher the average diameter of the inhibition zone is the same. Incubation temperature and volume affect the diameter of the antibiotic inhibition zone in the disc diffusion method for the antibiotic sensitivity test. It can be concluded that incubation temperature affects the diameter of the antibiotic inhibition zone in disc diffusion tests. It is recommended for future standardized and precise testing conditions to ensure accurate and reliable antibiotic sensitivity results.              
Sehat Tanpa Kanker Dengan Upaya Preventif Melalui Edukasi Dan Pemeriksaan Laboratorium Di Kampung Sidomulyo Rw 06 Kel. Bener, Kec. Tegalrejo Yogyakarta Nuryani, Siti; Harmilah, Harmilah; Suyono, Suyono; Kurniati, Eni; Sudaryanto, Sigit; Kasiyati, Menik
Sahabat Sosial: Jurnal Pengabdian Masyarakat Vol. 3 No. 2 (2025): Sahabat Sosial: Jurnal Pengabdian Masyarakat (Maret)
Publisher : Asosiasi Guru dan Dosen Seluruh Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59585/sosisabdimas.v3i2.629

Abstract

The community receives guidance and counseling about health and can develop a relationship with health initiatives, this can be designed in a higher education community with various activities that can contribute to community health. The purpose of PkM is to find out the knowledge of residents about cancer and to know Health through simple laboratory examinations through Preventive activities with education and Healthy Laboratory examinations in Sidomulyo Village RW 06 Kel. Bener, Tegalrejo District, Yogyakarta. The method used in the delivery is the lecture or counseling method to increase knowledge through pretests and posttests in measuring health status with general health examinations, and simple laboratory examinations. Residents of Sidomulyo Village, Bener Village, Tegalrejo District, Yogyakarta have never had counseling related to cancer in the past few years, and how much knowledge is not yet known. Based on this, it is necessary to hold counseling activities. Lecturers have the opportunity to carry out the Tri Dharma of Higher Education in the form of Community Service (PkM). The results of the activity are the implementation of PkM activities and increased knowledge about cancer. In addition, from 27 residents who were examined, it was found that 30% had high uric acid levels, 11% had high random blood sugar levels, 37% had high uric acid levels and 90% had high cholesterol.
Co-Authors Aeni, Ajeng Puspa Anung Sugihantono Azahra, Sresta Budi Setiawan Duri, Iin Desmiany Eni Kurniati, Eni Fardhiasih Dwi Astuti Fauzi, Afrizal Ahmad Harmilah, Harmilah Hartono Kahar, Hartono Jusak Nugraha Kuncara, Rachmad Bayu Martsiningsih, Marta Atik Martsiningsih, Martha Atik Muhammad Adnan Muji Rahayu Nugerohojati, Darojatun Nurul Qomariyah Pudyastuti, Rita Rena Rahmad Rahmawati, Ully Ramadania, Arindha Desyifa Ratih Hardisari, Ratih Ririh Jatmi Wikandari Sasangka, Parisade Galih Sistiyono Sistiyono, Sistiyono Siti Nuryani, Siti Sudaryanto, Sigit Sujono Sujono, Sujono Surati Surati Suyana Suyana, Suyana Suyono Suyono Tri Widada, Subrata Ullya Rahmawati Wiwit Sulistyasmi Zulfikar Husni Faruq