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Roni Koneri
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Jurnal Bios Logos
Published by Universitas Sam Ratulangi
ISSN : 20889569     EISSN : 26563282     DOI : https://doi.org/10.35799/jbl.11.2.2020.28153
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
JURNAL BIOS LOGOS is the journal published by Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University. The aims of the journal are to publish original research papers and article review in biology science i.e. botany, zoology, molecular biology, microbiology, ecology, diversity and conservation, taxonomy and biogeography. BIOS LOGOS is published two times per year (February and August)
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 5 Documents
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Search results for , issue "Vol 7, No 2 (2017): JURNAL BIOSLOGOS" : 5 Documents clear
Keanekaragaman Lamun di Pantai Kora-Kora, Kecamatan Lembean Timur Kabupaten Minahasa Sulawesi Utara (The Diversity of Seagrass in Kora-kora Beach, East Lembean District, Minahasa Regency, North Sulawesi Province) Wondal, Yuniarti Yurike; Rondonuwu, Sendy; Maabuat, Pience V
JURNAL BIOS LOGOS Vol 7, No 2 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.2.2017.18578

Abstract

Abstrak Penelitian dilakukan di Pantai Kora-Kora, Kecamatan Lembean Timur, Kabupaten Minahasa, Provinsi Sulawesi Utara dari Mei hingga Juni 2017. Penelitian bertujuan untuk menganalisis keanekaragaman lamun di Pantai Kora-Kora. Penelitian menggunakan metode purposive random sampling untuk menentukan lokasi penelitian. Hasil penelitian menunjukan bahwa di Pantai Kora-Kora, Kecamatan Lembean Timur ditemukan enam (6) jenis lamun yang termasuk dalam dua (2) suku yaitu Hidrocharitaceae meliputi Enhallus acoroides, Thallasia hemprichii, dan Halodule uninervis dan Cymodoceaceae yaitu Cydomocea rotundata, C. serrulata dan Syringodium isoetifolium.  Jenis-jenis tersebut ialah lamun yang tersebar di pesisir tropis Indo-Pasifik. Jenis yang berperan penting berdasarkan INP di Stasiun I, II dan III berturut-turut yaitu E. acoroides (58,50%), H. uninervis (58,60%) dan S. isoetifolium (63,0%). Indeks keanekaragaman jenis lamun di lokasi penelitian di Panta Kora-kora termasuk sedang (H’:1,71).   Kata kunci: Pantai Kora-Kora, keanekaragaman lamun. Abstract The research was conducted in the Kora-Kora Beach, East Lembean District, Minahasa Regency, North Sulawesi Province from May to June 2017. The aim of study was to analyze the diversity of seagrass on Kora-Kora Beach. The purposive random sampling method was used in this research to determine the research locations. The results showed that Kora-Kora Beach had six (6) seagrass species that were included in two (2) families namely Hidrocharitaceae ( Enhallus acoroides, Thallasia hemprichii, Halodule uninervis) and Cymodoceaceae (Cydomocea. rotundata, C. serrulata and Syringodium isoetifolium). These seagrass species were found in the tropical Indo-Pacific coastlines. The important species based on Importance Value Index  in Station I, II and III were E. acoroides (58.50%), H. uninervis (58.60%) and S. isoetifolium (63.0%), respectively. The seagrass species diversity index in the study areas in the Kora-kora Beach was moderate (H ': 1.71).Keywords:  Kora-Kora Beach, seagrass diversity.
Respon Morfologis dan Ekspresi Gen Aquaporin pada Padi IR 64 yang Mengalami Cekaman Kekeringan pada Fase Reproduktif (Morphological Responses and Aquaporin Gene Expression in Rice IR64 under Drought Stress at the Reproductive Stage) Pharmawati, Made; Wirasiti, Ni Nyoman; Wrasiati, Luh Putu
JURNAL BIOS LOGOS Vol 7, No 2 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.2.2017.18579

Abstract

Abstrak Cekaman kekeringan merupakan faktor pembatas penting bagi pertumbuhan dan produktivitas tanaman termasuk padi.      Penelitian ini bertujuan menganalisis respon padi IR64 terhadap cekaman kekeringan dengan pemberian polietilen glikol (PEG) pada fase reproduktif.  Penelitian juga bertujuan menganalisis ekspresi gen aquaporin akibat cekaman kekeringan.  Bibit padi ditanam dalam pot dan perlakuan PEG dengan konsentrasi 108g/L (-0.25MPa) dan 178g/L (-0.52 MPa) diberikan saat munculnya panikula. Perlakuan diberikan selama 2 minggu, kemudian tanaman disiram kembali.  Ekspresi gen diamati pada akhir perlakuan dengan semi kuantitatif real time PCR.  Ekstraksi RNA menggunakan RNeasy plant mini kit, sedangkan sintesis cDNA menggunakan Transcriptor First Strand cDNA Kit.  Hasil penelitian menunjukkan bahwa jumlah malai dan berat total malai berkurang akibat cekaman kekeringan.  Persentase gabah kosong mencapai 84,6% pada perlakuan PEG-0,52 MPa, sedangkan pada perlakuan PEG -0,25 MPa persentase gabah kosong sebesar 67,8%.  Pada kontrol persentase gabah kosong adalah 10,3%.  Ekspresi gen OsPIP2;7 sedikit menurun pada perlakuan PEG -0,52 MPa.Kata kunci: ekspresi gen, IR64, kekeringan, padi, PEG  Abstract Drought stress is one of the limiting factors of plant growth and productivity including rice.  The aim of this study was to analyze responses of IR64 rice to polyethylene glycol (PEG)-induced-drought stress at the reproductive stage.  This study also aimed to analyze the expression of aquaporin under drought stress.  Rice seedlings were grown in pot system and PEG treatment at concentration of -0.25MPa (108g/L) and -0.52 MPa (178g/L) were given when the panicles arose.  Treatments were conducted for 2 weeks, after that the plants were rewatered.  Gene expression was evaluated at the end of PEG treatment using semi quantitative real time PCR. RNA was extracted using RNeasy plant mini kit, while cDNA synthesis was done using Transcriptor First Strand cDNA Kit.  The results showed that the number and weight of rice ear were less in plant treated with PEG than in control.  The percentage of empty rice grain reached 84.6% at PEG -0.52 MPa, while at PEG -0.25 MPa the percentage of empty grain was 67.8%.  In control plant, the percentage of empty grain was 10.3%.  Drought stress did not alter the expression of OsPIP2;7.  Keywords: drought, gene expression, IR64, PEG, rice
Analisis Bakteri secara Kuantitatif pada Jajanan Kue Ku di Pasar Tradisional Bersehati Kota Manado (Quantitative Bacterial Analysis of “Kue Ku” in Bersehati Traditional Market Manado City) Laiya, Nurpratiwi; Pelealu, Johanis J; Singkoh, Marina FO
JURNAL BIOS LOGOS Vol 7, No 2 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.2.2017.18577

Abstract

Abstrak             Pangan jajanan masih beresiko terhadap kesehatan karena penanganannya sering tidak higienis, yang memungkinkan jajanan Kue Ku terkontaminasi mikroba. Penelitian ini bertujuan untuk menganalisis bakteri secara kuantitatif pada jajanan Kue Ku di Pasar Tradisional Bersehati Kota Manado. Penelitian ini dilakukan dengan cara mengisolasi bakteri pada medium diferensial PCA (Plate Count Agar) selama 48 jam pada suhu 37°C kemudian dimurnikan lagi dengan medium selektif MCA (Mac Conkey Agar) dan medium diferensial NA (Nutrient Agar). Hasil penelitian menunjukkan bahwa analisis kuantitas bakteri pada jajanan Kue Ku di Pasar Tradisional Bersehati Kota Manado telah memenuhi syarat mutu batas maksimum cemaran mikroba yaitu pada tempat 1 berkisar 4 x 101 CFU/mL  pada tempat 2 berkisar 3 x 101 CFU/mL dan pada tempat 3 berkisar 0,003 x 103 CFU/mL. Kata kunci: bakteri, koloni, kue jajanan Abstract “Kue Ku” as a kind of traditional cake is able to be contaminated by microbes because of unhygienic handling. This study aimed to analyze the bacteria quntitatively on “Kue Ku” cakes in Bersehati Traditional Market, Manado City. This study was conducted by isolating bacteria on PCA (Plate Count Agar) differential medium for 48 hours at 37 ° C then purified again with MCA (Mac Conkey Agar) selective medium and NA (Nutrient Agar) differential medium. The results showed that the number of bacteria on “Kue Ku” cake at Bersehati Traditional Market, Manado City fulfilled the quality requirement of maximum limit of microbial contamination, i.e. 4 x 101 CFU/mL (location 1), 3 x 101 CFU/mL (location 2), and 0.003 x 103 CFU/mL (location 3) . Keywords: bacteria, colony, traditional cake
Biodiversitas Mangrove di Pulau Mansuar Kabupaten Raja Ampat Provinsi Papua Barat (The Biodiversity of Mangrove in the Mansuar Island Raja Ampat District West Papua Province) Mayor, Troce; Simbala, Herny EI; Koneri, Roni
JURNAL BIOS LOGOS Vol 7, No 2 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.2.2017.18576

Abstract

Abstrak            Hutan mangrove merupakan formasi hutan yang tumbuh dan berkembang pada daerah landai di muara sungai dan pesisir pantai yang dipengaruhi oleh pasang surut air laut dan pantai berlumpur. Penelitian ini bertujuan untuk menganalisis biodiversitas mangrove di Pulau Mansuar Kabupaten Raja Ampat, Provinsi Papua Barat. Metode yang digunakan yaitu transek kuadrat. Hasil penelitian ditemukan sebanyak 6 famili 11 spesies dan 554 individu mangrove. Komposisi individu tertinggi dimiliki oleh spesies Rhizophora apiculata, sedangkan terendah yaitu spesies Xylocarpus rumpii. Indeks keanekaragaman, kekayaan, dan kelimpahan spesies mangrove pada semua tingkat pertumbuhan tertinggi pada stasiun 3, sedangkan terendah pada stasiun 1. Berdasarkan besaran kriteria yang dikemukakan oleh Shannon-Weiner maka indeks keanekaragaman mangrove di Pantai Yenbubua, Kepulauan Mansuar tergolong kategori sedang. Kata kunci: biodiversitas, kekayaan spesies, Pulau Mansuar, Rhizophora apiculata. Abstract Mangrove forests are forest formations that grow and flourish in slopes at the mouth of the rivers and coastal areas that are affected by tidal sea water and muddy beaches. This study aimed to analyze the biodiversity of mangroves in Mansuar Island, Raja Ampat District, West Papua Province. The method used was quadrat transects. The results of the study showed that there were 6 families of 11 species and 554 individuals. The highest composition of the individual mangrove species was Rhizophora apiculata, while the lowest composition was Xylocarpus rumpii.  The highest diversity index, richness, and abudance spesies in a various growth rate were observed in the station 3, while these lowest parameters were found in the station 1. Based on the magnitude of the criteria proposed by Shannon-Weiner, the index of mangrove diversity in Yenbubua Beach, Mansuar Islands was classified as medium category. Keywords: biodiversity, Mansuar Island, Rhizophora apiculata, species richness    
Isolasi dan Identifikasi Bakteri Rizosfir Arachis pintoi setelah Inokulasi Mikoriza Arbuskular dan Penambahan Pupuk Organik (Isolation and Identification of Rhizosphere Bacteria in Arachis pintoi after the Inoculation of Arbuscular Mycorrhiza and The Addition of Organic Fertilizer) Pelealu, Johan Bhayangkara; Butarbutar, Regina Rosita; Tallei, Trina E
JURNAL BIOS LOGOS Vol 7, No 2 (2017): JURNAL BIOSLOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.7.2.2017.18575

Abstract

Abstrak Arachis pintoi (kacang pinto) sering digunakan sebagai pakan ternak, memiliki kemampuan hidup pada lahan marginal, dan mampu mengembalikan indeks kualitas tanah dari sangat rendah menjadi sedang tinggi, karena adanya bakteri dan fungi yang berperan di lingkungan rizosfir. Penelitian ini bertujuan untuk mengidentifikasi bakteri rizosfir A. pintoi setelah inokulasi mikoriza arbuskular dan penambahan pupuk organik. Kacang pinto ditanam pada tanah yang diberi 20 g mikoriza arbuskular dan 500 g pupuk organik untuk setiap 3 kg tanah di dalam polibag. Setelah tanaman berusia 6 minggu, sebanyak 10 g tanah diambil dari lingkungan rizosfir kemudian dimasukan ke dalam gelas Erlenmeyer dan ditambahkan dengan 90 ml air suling steril. Sebanyak 1 ml suspensi hasil pengenceran 10-5 ditebar di atas media Nutrient Agar. Setiap koloni yang tumbuh dimurnikan dan diidentifikasi secara biokimiawi. Dari hasil identifikasi diperoleh 7 jenis bakteri rizosfir yaitu Azotobacter sp., Enterobacter sp., Rhizobium sp., Enterebocter sp., Azospirillum sp., Pseudomonas sp., dan Bacillus sp.Kata kunci: rizosfir, mikoriza arbuskular, Arachis pintoi, kacang pinto           Abstract Arachis pintoi (pinto bean) is often used as animal feed, has the ability to live on marginal land, and is able to provide soil quality from very low to high, due to the presence of bacteria and fungi that play a role in the rhizosphere. This study aims to identify the rhizosphere bacteria of A. pintoi after inoculation of arbuscular mycorrhizae and the addition of organic fertilizer. The pinto beans are grown on soil that has been given 20 g of arbuscular mycorrhizal and 500 g organic fertilizer for every 3 kg of soil in polybags. After the plants were 6 weeks old, 10 g of soil was taken from the rhizosphere environment then placed into the Erlenmeyer glass and added with 90 ml of sterile distilled water. A total of 1 ml 10-5 dilution, the suspension was spread over onto Nutrient Agar medium. Each growing colony is purified and identified biochemically. From this, 7 types of rhizosphere bacteria was identified, namely Azotobacter sp., Enterobacter sp., Rhizobium sp., Enterebocter sp., Azospirillum sp., Pseudomonas sp., and Bacillus sp.Keywords: rhizosphere, arbuscular mycorrhizae, Arachis pintoi, pinto bean

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