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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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Search results for , issue "Vol. 1 No. 1 (2014): December 2014" : 20 Documents clear
KEMAMPUAN TUMBUH EKSPLAN Jatropha curcas L. PADA MEDIA IN VITRO YANG MENGANDUNG HORMON IBA DAN BA ., Karyanti; ., Juanda; Tajuddin, Teuku
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.633 KB) | DOI: 10.29122/jbbi.v1i1.545

Abstract

GROWTH ABILITY OF Jatropha curcas L. EXPLANTS ON THE IN VITRO MEDIA CONTAINING IBA AND BAResearch on the growth ability of Jatropha curcas L. shoots and callus in solid and liquid media have been conducted. Explants were planted in the initiation MS medium. After ten weeks, the explants were subcultured into solid and liquid media containing combination of IBA and BA treatments. The number of combinations was 12 treatments, each with 6 replications. Observation was conducted from the first week after subculturing upto the fourth week. Parameters of observation were the percentage of explant forming shoots, the number of shoots, height, number of leaves, weight, color, and form of callus. The results showed that the explant which was subcultured in liquid media had higher growth rate than those subcultured in solid media. Treatment of 1 ppm IBA + 0.5 ppm BA gave a good result on the growth of shoots on solid and liquid media. For callus formation, treatment of 2 ppm IBA + 1 ppm BA gave the best result.Keywords: Callus, Jatropha, IBA and BA, solid and liquid media, hormone ABSTRAKPenelitian terhadap kemampuan tumbuh kalus dan tunas tanaman jarak pagar (Jatropha curcas L.) telah dilakukan pada media padat dan cair. Eksplan diinisiasi pada media MS dan setelah 10 minggu dipindahkan ke media padat dan cair yang mengandung perlakuan kombinasi hormon IBA dan BA. Jumlah kombinasi sebanyak 12 perlakuan dan setiap perlakuan dibuat 6 ulangan. Pengamatan dilakukan dari minggu pertama subkultur hingga minggu keempat. Peubah yang diamati adalah persentase eksplan yang membentuk tunas, jumlah dan tinggi tunas, terbentuknya daun pada tunas, perbedaan berat, bentuk, dan warna kalus. Hasil yang diperoleh menunjukkan bahwa eksplan yang disubkultur pada media cair memiliki laju pertumbuhan yang lebih tinggi daripada media padat. Perlakuan IBA 1 ppm + BA 0,5 ppm menghasilkan pertumbuhan tunas yang paling tinggi pada media padat dan cair. Pembentukan kalus yang terbaik diperoleh pada perlakuan IBA 2 ppm + BA 1 ppm.Kata kunci: Kalus, Jatropha, IBA dan BA, media padat dan cair, hormon
TINJAUAN LOVASTATIN DAN APLIKASINYA Hardianto, Dudi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (909.8 KB) | DOI: 10.29122/jbbi.v1i1.550

Abstract

Lovastatin is a drug belonging to statins group that is used to decrease the cholesterol levels in blood. The action mechanism of lovastatin is inhibition of the activity of HMG-CoA reductase enzyme, hence reducing cholesterol production in the liver. Some filamentous fungi produce lovastatin, and Aspergillus terreus is known as the highest lovastatin-producing filamentous fungi, therefore it is generally used for production of lovastatin. Commercial production of lovastatin is based on submerged fermentation. But nowadays solid-state fermentation is becoming an alternative for production of lovastatin. Lovastatin is mainly used for antihypercholeterolemia. Other potential uses of lovastatin include therapy of Alzheimer’s disease, cancer, osteoporosis, Parkinson’s disease, multiple sclerosis, and rheumatoid arthritis.Keywords: Statin, lovastatin, Aspergillus terreus, fermentation, antihypercholeterolemia ABSTRAK Lovastatin merupakan obat golongan statin yang digunakan untuk menurunkan kadar kolesterol dalam darah. Mekanisme kerja lovastatin adalah menghambat enzim HMG-CoA reduktase sehingga produksi kolesterol di dalam hati berkurang. Beberapa kapang berfilamen memproduksi lovastatin dan Aspergillus terreus merupakan kapang penghasil lovastatin tertinggi sehingga digunakan dalam produksi lovastatin. Produksi lovastatin secara komersial menggunakan fermentasi cair tetapi sekarang ini fermentasi padat menjadi alternatif lain untuk memproduksi lovastatin. Lovastatin digunakan terutama untuk antihiperkolesterolemia. Lovastatin juga potensial digunakan untuk pengobatan penyakit alzheimer, kanker, osteoporosis, parkinson, multiple sclerosis, dan rheumatoid arthritis.Kata kunci: Statin, lovastatin, Aspergillus terreus, fermentasi, antihypercholeterolemia
UJI KEMAMPUAN Lactobacillus casei SEBAGAI AGENSIA PROBIOTIK Sunaryanto, Rofiq; Martius, Efrida; Marwoto, Bambang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.06 KB) | DOI: 10.29122/jbbi.v1i1.546

Abstract

Growth Ability of Jatropha Curcas L. Explants on the In Vitro Media Containing IBA and BAProbiotic product is one of the applications of biotechnology that utilize lactic acid bacteria, especially lactobacilli. Some important requirements for microbes that can be used as probiotic include resistance to low pH, ability to grow on bile salts and colonize, and having antimicrobial activity. Each species of the genus Lactobacillus has different characteristics. These characteristics are strongly influenced by the environment in which the bacteria live. This study was carried out in order to characterize Lactobacillus casei which was isolated from dadih. The result of the experiment showed that the isolated L. casei was able to grow on the bile salt at the concentration of 15%, resistant to acid media until pH 2, had antimicrobial activity (significantly inhibited the growth of Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis). The local L. casei isolate has a potential application for use as probiotic microbe.Keywords: Lactobacillus casei, probiotic, lactic acid bacteria, characterization,dadih ABSTRAKProduk probiotik merupakan salah satu aplikasi bioteknologi yang memanfaatkan bakteri asam laktat terutama jenis Lactobacillus. Beberapa syarat utama mikroba yang dapat difungsikan sebagai mikroba probiotik antara lain tahan terhadap pH rendah, mampu tumbuh pada garam empedu, mampu berkoloni, memiliki aktivitas antimikroba. Masing-masing spesies dari Genus Lactobacillus memiliki karakteristik yang berbeda-beda. Karakteristik ini sangat dipengaruhi oleh lingkungan dimana bakteri tersebut hidup. Pada penelitian ini telah dilakukan karakterisasi Lactobacillus casei yang merupakan hasil isolasi dari susu kerbau fermentasi. Dari hasil percobaan menunjukkan bahwa L.casei hasil isolasi mampu hidup sampai dengan konsentrasi garam empedu 15%, tahan terhadap media asam sampai dengan pH 2, memiliki aktivitas antimikroba (positif menghambat Escherichia coli, Staphylococcus aureus, dan Enterococcus faecalis). L. casei yang merupakan isolat lokal memiliki karakteristik yang berpotensi untuk digunakan sebagai mikroba probiotik.Kata kunci: Lactobacillus casei, probiotik, bakteri asam laktat, karakterisasi, dadih
ANALISA KANDUNGAN ANDROGRAPHOLIDE PADA TANAMAN SAMBILOTO (Andrographis paniculata) DARI 12 LOKASI DI PULAU JAWA Royani, Juwartina Ida; Hardianto, Dudi; Wahyuni, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (842.933 KB) | DOI: 10.29122/jbbi.v1i1.547

Abstract

Concentration of active compounds contained in medicinal plants is determined by genetic factors as well as growth environment. In sambiloto plants both factors have major impacts on the formation of diterpene lactone, andrographolide. Variation of sampling time, cultivation, and processing methods causes variation in the content of active compounds of the same plant. The purpose of this study was to determine andrographolide concentration of sambiloto plants obtained from 12 different locations with various planting conditions in Java Island. andrographolide content of sambiloto was extracted by methanol and analyzed using HPLC. The results showed that the concentrations of andrographolide varied from 0.29 to 4.44% with an average of 2.19% on dry weight basis. The highest concentration of 4.44% was detected in sambiloto accession from Wonokaton Village, Pasuruan Regency while the lowest one was from Conggeang Kulon Village, Sumedang Regency. Three sambiloto accessions had potential to be further developed as their andrographolide concentrations were above 3%, which was higher than those from all the others.Keywords: Andrographis paniculata, andrographolide, active coumpound, HPLC, Java island ABSTRAKKadar senyawa aktif yang terkandung pada tanaman obat selain dipengaruhi oleh faktor genetik juga dipengaruhi oleh faktor lingkungan tumbuhnya. Pada tanaman sambiloto kedua faktor tersebut berpengaruh sangat besar pada pembentukan diterpen lakton, andrographolide. Adanya variasi pada waktu pengambilan sampel, tempat penanaman, metode pengolahan dan lain sebagainya berakibat pada perbedaan dalam kandungan senyawa aktif pada tanaman yang sama. Tujuan dari penelitian ini adalah untuk mengetahui kadar andrographolide dari tanaman sambiloto yang diambil dari 12 lokasi tumbuh dengan kondisi penanaman yang berbeda di Pulau Jawa. Daun tanaman sambiloto diekstrak dengan methanol kemudian dianalisis kandungan andrographolide menggunakan HPLC. Kadar andrographolide yang dihasilkan bervariasi berkisar antara 0,29-4,44% dengan kadar rata-rata adalah 2,19% berat kering. Kadar tertinggi didapatkan pada aksesi dari Desa Wonokaton Kabupaten Pasuruan dengan kadar andrographolide adalah 4,44% sedangkan kadar yang terendah didapatkan pada aksesi dari Desa Conggeang Kulon, Kab. Sumedang. Berdasarkan data kandungan andrographolide, diperoleh 3 aksesi sambiloto yang potensial untuk dikembangkan menjadi aksesi unggulan karena kadar andrographolidenya di atas 3%, melebihi semua yang lain.Kata kunci: Andrographis paniculata, andrographolide, senyawa aktif, HPLC, pulau Jawa
Preface JBBI Vol 1, No 1, December 2014: Foreword and Acknowledgement Tajuddin, Teuku
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.781 KB) | DOI: 10.29122/jbbi.v1i1.1040

Abstract

A REVISED METHOD FOR SUCKER STERILIZATION TO SUPPORT THE IN VITRO PROPAGATION OF SAGO PALM (Metroxylon sagu Rottb.) Tajuddin, Teuku; ., Karyanti; Sukarnih, Tati; Haska, Nadirman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.57 KB) | DOI: 10.29122/jbbi.v1i1.548

Abstract

Hutan sagu (Metroxylon sagu Rottb.) dapat ditemukan dalam area yang cukup luas di wilayah Maluku dan Papua. Besarnya keanekaragaman hayati dari pohon sagu dapat dilihat di areal ini. Pohon sagu tumbuh secara alami terutama di daerah dataran atau rawa dengan sumber yang air melimpah. Tanaman sagu dapat diperbanyak dengan metode generatif melalui biji, dan vegetatif melalui tunas anakan. Dalam rangka mendukung perbanyakan pohon induk yang unggul secara in vitro dalam skala besar, perbaikan metode sterilisasi tunas anakan mutlak diperlukan. Tunas anakan muda (15-20 cm) yang diperoleh dari Propinsi Papua digunakan sebagai eksplan. Tujuan percobaan sterilisasi ini dilakukan untuk mendukung perbanyakan pohon sagu secara in vitro. Pada percobaan ini antibiotik digunakan untuk membersihkan jaringan internal eksplan dari jamur dan bakteri. Hasil percobaan ini menunjukkan bahwa campuran alkohol dan antibiotik dapat menekan pertumbuhan kontaminan.Kata kunci: Antibiotik, kontaminan jamur dan bakteri, kultur in vitro, metode sterilisasi, sagu ABSTRACTNatural sago (Metroxylon sagu Rottb.) forest can be found in large area in Maluku and Papua regions. There are wide genetic diversities of sago palm found in these areas. This palm grows along riverbanks and in swampy areas which are not suitable for other crops. Sago palm is propagated generatively by seed and vegetatively by suckers. With the purpose of establishing the in vitro culture method for a large-scale of mass clonally propagation of superior genotypes of sago palm, generating sterilized explants are very important. Young suckers (15-20 cm) obtained from areas of Papua Province were used as explants. The sterilization experiments were carrying out to support the tissue culture of sago palm. Sterilization was conducted using antibiotics in order to get rid of fungi and bacteria from inner part of explants tissues. The results showed that from all sterilization methods tested, the best result was treatment using alcohol and antibiotic as disinfectant agents.Keywords: Antibiotics, fungi and bacteria contaminants, in vitro culture, sterilization method, sago palm
Back Cover JBBI Vol 1, No 1, December 2014 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.652 KB) | DOI: 10.29122/jbbi.v1i1.1039

Abstract

Front Cover JBBI Vol 1, No 1, December 2014 Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 20140
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (905.939 KB) | DOI: 10.29122/jbbi.v1i1.1038

Abstract

PERTUMBUHAN ISOLAT BAL ASAL BEKATUL DAN PROBIOTIK KOMERSIAL (Lactobacillus acidophilus dan Lactobacillus casei) PADA MEDIA BEKATUL DAN SUSU SKIM Zubaidah, Elok; Martati, Erryana; Resmanto, Ampu Marojahan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1098.558 KB) | DOI: 10.29122/jbbi.v1i1.549

Abstract

This research was aimed to study the influence of rice bran and skim milk fermentation media on the growth of lactic acid bacteria and their ability in fermenting complex carbohydrates into short chain fatty acids (SCFA). Indigenous lactic acid bacteria (LAB) were isolated from rice bran and commercial probiotic separately and used for fermenting rice bran and skim milk media. Randomized block design was used with 2 factors i.e. fermenting media type and LAB type. The results showed that fermenting rice bran gave significant effect on the LAB growth, indicated by total LAB cell count, total acid concentration, pH and antibacterial activity. The best treatment was J2-B with total LAB count 1.01 ´ 1010 cfu/mL, total acid 1.14%, pH 3.88 and clear zone diameters against Staphylococcus aureus 13.04 mm, Listeria monocytogenes 12.88 mm, Escherichia coli 12.83 mm and Salmonella typhi 12.53 mm. LAB fermenting rice bran for 48 hours produced lactic acid and SCFA. The highest concentrations of lactic acid (122.1313 mM), acetic acid (10.503 mM), and butyric acid (1.56 mM) were produced by fermentation using LAB J2, L. acidophilus, and L. casei isolate, respectively; whereas the highest propionic acid concentration (6,07 mM) was produced by control fermentation.Keywords: Probiotic, indigenous isolate, rice bran, SCFA, skimmed milk ABSTRAKPeneltian ini bertujuan untuk mengetahui pengaruh dedak dan skim milk sebagai media fermentasi bakteri asam laktat, dan kemampuannya mengubah sumber karbon komplek dedak menjadi asam lemak rantai pendek (short chain fatty acids, SCFA). Bakteri asam laktat lokal diisolasi dari dedak dan probiotik. Desain percobaan adalah acak kelompok dengan 2 faktor, yaitu jenis media fermentasi dan jenis bakteri asam laktat. Hasil penelitian menunjukkan bahwa media fermentasi dengan menggunakan dedak menunjukkan pengaruh yang signifikan terhadap pertumbuhan bakteri yang ditunjukkan dari total sel bakteri asam laktat, total asam yang dihasilkan, pH dan aktivitas antibakteri. Fermentasi dengan menggunakan isolat J2-B menghasilkan total bakteri asam laktat 1,01 ´ 1010 cfu/mL, total asam 1,14%, pH 3,88 dan zona hambatan dengan bakteri uji Staphylococcus aureus 13,04 mm, Listeria monocytogenes 12,88 mm, Escherichia coli 12,83 mm dan Salmonella typhi 12,53 mm. Proses fermentasi bakteri asam laktat menggunakan media dedak selama 48 jam mampu menghasilkan asam laktat dan SCFA. Konsentrasi tertinggi asam laktat (122,13 mM), asam asetat (10,50 mM), dan asam butirat (1,56 mM) masing-masing dihasilkan oleh fermentasi menggunakan BAL J2, isolat L. acidophilus, dan isolat L. casei; sedangkan konsentrasi tertinggi asam propionat (6,07 mM) dihasilkan oleh fermentasi kontrol.Kata kunci: Probiotik, isolat lokal, dedak, SCFA, susu skim
Appendix JBBI Vol 1, No 1, December 2014: Keyword Index and Author Index Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 1 No. 1 (2014): December 2014
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.717 KB) | DOI: 10.29122/jbbi.v1i1.1042

Abstract

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