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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
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DEKSTROSA MONOHIDRAT KUALITAS FARMASI DARI PATI Manihot ecsulenta, Metroxylon sagu, Zea mays, Oryza sativa, dan Triticum Bayu Mahdi Kartika; Lely Khojayanti; . Nuha; Shelvi Listiana; Susi Kusumaningrum; Ayustiyan Futu Wijaya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1230.738 KB) | DOI: 10.29122/jbbi.v6i2.3208

Abstract

Pharmaceutical Grade Dextrose Monohydrate from Manihot ecsulenta, Metroxylon sagu, Zea mays, Oryza sativa, dan Triticum Starch ABSTRACT Pharmaceutical-grade dextrose monohydrate, one of raw materials used as active pharmaceutical ingredients (API) and additives, can be made from starch. There are five types of local Indonesian commercial starch that are potentially used, namely tapioca (Manihot esculenta), sago (Metroxylon sagu), corn (Zea mays), rice (Oryza sativa), and wheat (Triticum) starch. This study aimed to compare these five starches as raw materials for preparing pharmaceutical-grade dextrose monohydrate which was expected to meet the requirements of the Indonesian Pharmacopoeia (5th Edition) and the United States Pharmacopeia (USP). The starch was converted into dextrose monohydrate through liquefaction hydrolysis, saccharification hydrolysis, activated carbon purification and filtration, ion exchange purification, evaporation, crystallization and drying.  High Performance Liquid Chromatogram (HPLC) and the Luff-Schoorl methods were used for dextrose equivalent value (DE) analysis. The results showed that only three of the starch types produced pharmaceutical-grade dextrose monohydrate, namely (DE) sago starch (107.23% and 100.77%), corn starch (97.86% and 96.19%), and tapioca starch (85.18% and 99.20%).Keywords: dextrose equivalent, dextrose monohydrate, hydrolysis, pharmaceutical grade, starchABSTRAKDekstrosa monohidrat kualitas farmasi, salah satu bahan baku yang digunakan sebagai active pharmaceutical ingredient (API) dan bahan tambahan, dapat dibuat dari bahan pati-patian. Terdapat lima jenis pati komersial lokal Indonesia yang berpotensi digunakan yakni pati tapioka (Manihot esculenta), pati sagu (Metroxylon sagu), pati jagung (Zea mays), pati beras (Oryza sativa), dan pati gandum (Triticum). Penelitian ini bertujuan membandingkan lima jenis pati tersebut sebagai bahan baku pembuatan dekstrosa monohidrat kualitas farmasi yang diharapkan mampu memenuhi standar persyaratan dari Farmakope Indonesia Edisi V dan United States Pharmacopeia (USP). Pati diubah menjadi dekstrosa monohidrat melalui hidrolisis likuifikasi, hidrolisis sakarifikasi, pemurnian karbon aktif dan filtrasi, pemurnian ion exchange, evaporasi, kristalisasi dan pengeringan. Metode High Performance Liquid Chromatogram (HPLC) dan Luff-Schoorl digunakan untuk analisis dextrose equivalent (DE). Hasil penelitian menunjukkan hanya tiga jenis pati yang menghasilkan dekstrosa monohidrat kualitas farmasi, yakni (DE) pati sagu (107,23% dan 100,77%), pati jagung (97,86% dan 96,19%), dan pati tapioka (85,18% dan 99,20%).Kata kunci: dekstrosa monohidrat, dextrose ekuivalen, hidrolisis, kualitas farmasi, pati
OPTIMASI PERMUKAAN RESPONS MEDIUM FERMENTASI Streptomyces prasinopilosus SEBAGAI ANTIFUNGI TERHADAP PATOGEN Ganoderma boninense Rofiq Sunaryanto; Diana Nurani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1326.321 KB) | DOI: 10.29122/jbbi.v6i2.3231

Abstract

Response Surface Optimization of Medium Fermentation for Streptomyces prasinopilosus as An Antifungal against Ganoderma boninenseGanoderma boninense is one of the pathogenic fungi that cause basal stem rot (BPB) on oil palm plants. This research aims to study the effect of carbon sources, nitrogen sources, and minerals on the production of Streptomyces prasinopilosus active compounds. Lactose, yeast extract, and minerals are medium components that show a real influence on the production of S. prasinopilosus active compounds. Optimization of the factors that have significant influence was predicted by the second-order model, statistically through a central composite design (CCD). The highest S. prasinopilosus active compound production, with a medium composition of 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution, was predicted by the quadratic model to reach 32269366.338 peak area unit on high-performance liquid chromatography (HPLC). The verification of the mathematical model of the production of the active compounds through experiments in the laboratory was 27,203,907.310 peak area unit. This result was 15.7% lower compared to the result of the quadratic model. Optimization increased S. prasinopilosus active compound 9-fold compared to that before optimization.Keywords: active compound; G. boninense; optimization; RSM; S. prasinopilosus ABSTRAKGanoderma boninense merupakan salah satu jamur patogen yang menyebabkan penyakit busuk pangkal batang atau biasa disebut BPB pada tanaman kelapa sawit. Penelitian bertujuan mempelajari pengaruh sumber karbon, sumber nitrogen, dan mineral terhadap produksi senyawa aktif S. prasinopilosus. Laktosa, yeast extract, dan mineral adalah komponen medium yang menunjukkan pengaruh nyata terhadap produksi senyawa aktif S. prasinopilosus. Optimasi terhadap faktor yang berpengaruh nyata diprediksi dengan model orde dua melalui rancangan statistis central composite design (CCD). Produksi senyawa aktif S. prasinopilosus tertinggi diprediksi oleh model kuadratik mencapai 32269366,338 luasan puncak kromatografi cair kinerja tinggi (KCKT) dengan komposisi medium laktosa 44,77 g L-1, yeast extract 13,02 g L-1, dan larutan mineral 15,95 mL L-1. Verifikasi model matematis produksi senyawa aktif yang dihasilkan melalui percobaan di laboratorium adalah sebesar 27.203.907,310 luasan puncak kromatogram KCKT. Hasil ini lebih rendah 15,7% dibandingkan dengan model kuadratik hasil optimasi. Optimasi meningkatkan senyawa aktif S. prasinopilosus 9 kali lipat dibandingkan sebelum optimasi.
OPTIMASI TEKNIK WESTERN BLOT UNTUK DETEKSI EKSPRESI PROTEIN TANAMAN PADI (Oryza sativa L.) . Susianti; Edi Sukmana; Ronny Lesmana; Unang Supratman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.722 KB) | DOI: 10.29122/jbbi.v6i2.3249

Abstract

Optimization of Western Blot Technique for Protein Expression of Rice Plant (Oryza sativa L.) Western blot (WB) technique has been widely used for analyzing protein expression and for identifying specific proteins derived from animals, plants, and microorganisms. During the use of WB, especially in agricultural studies, some difficulties are encountered such as unclear or unspecific protein bands, the presence of bubbles, and the absence of protein bands on membrane. This study aims to determine the WB conditions appropriate for the protein expression of rice plants (Oryza sativa L.). Protein from rice plant was extracted and the obtained protein lysate was then used for proteomic analysis using western blot with β-actin antibody. Our experiment showed that some optimized parameters like blocking buffers, the concentration of primary antibody and the ratio of secondary antibody determined the clarity of the results. β-actin was used as internal control that measured the success of the WB technique. Results showed that lysis process was important in determining good WB results in addition to the optimal blocking solution using a BSA of 0.2%, a primary antibody concentration of 1 μg mL–1, and a secondary antibody of 1:10,000. Optimizing techniques during extraction, incubation, and documentation facilitated good WB results.Keywords: β-actin; optimization; protein; rice plant; western blotABSTRAKTeknik western blot (WB) telah banyak digunakan untuk analisis ekspresi protein dan mengidentifikasi protein spesifik dari hewan, tumbuhan dan mikroorganisme. Dalam implementasi teknik WB, khususnya studi dalam bidang pertanian, beberapa kesulitan ditemui seperti pita protein tidak jelas, tidak spesifik, adanya gelembung, hingga tidak munculnya pita protein pada membran. Penelitian ini bertujuan untuk mengetahui kondisi WB yang tepat untuk deteksi protein tanaman padi (Oryza sativa L.). Protein tanaman padi diekstraksi, kemudian lysate protein yang didapat dianalisis dengan metode westernblot menggunakan antibody β-actin. Penelitian kami menunjukkan bahwa beberapa parameter yang dioptimasi seperti larutan blocking, konsentrasi antibodi primer dan rasio antibodi sekunder akan menentukan hasil yang jelas. β-actin digunakan sebagai kontrol internal yang menjadi tolok ukur keberhasilan teknik WB. Hasil menunjukkan bahwa proses lisis menjadi hal penting dalam menentukan hasil WB yang baik disamping larutan blocking yang optimal menggunakan BSA 0,2%, konsentrasi antibodi primer 1 µg mL–1 dan antibodi sekunder 1:10.000. Mengoptimalkan teknik selama ekstraksi, inkubasi dan dokumentasi membantu mendapatkan hasil WB yang baik.
PENGARUH MEDIA DASAR DAN NAA PADA INDUKSI IN VITRO AKAR TUNAS KELAPA SAWIT (Elaeis guineensis) . Karyanti; Mutia Afifah; Tati Sukarnih; Yayan Rudiyana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (660.696 KB) | DOI: 10.29122/jbbi.v6i2.3476

Abstract

The Effect of Basal Media and NAA on the In Vitro Induction of Oil Palm (Elaeis guineensis Jacq.) RootABSTRACTClonal propagation of oil palm plants using tissue culture technique results in a low percentage of rooted shoots. To increase the percentage of rooted shoots that are more uniform, the root induction method is supported by the use of basic media and the addition of growth regulators 1-naphthaleneacetic acid (NAA). This study aims to analyze the effect of a combination of base media and optimum NAA concentration in inducing the roots of oil palm shoots in vitro. This research used factorial completely randomized design (RAL) consisting of 2 factors. The first factor was the type of basic media, namely Murashige and Skoog (MS) and MS Modifications (MSM) media. The second factor was the concentration of NAA, namely 0; 0.05; 0.1; and 0.2 ppm. each treatment was repeated 10 times. The results showed that the use of MSM medium was better than that of MS, and the most optimum NAA concentration was 0.05 and 0.1 ppm, in inducing oil palm roots in vitro. In addition, the combination of MSM + NAA 0.1 ppm treatment produced the most optimum result in induction of oil palm roots in vitro.Keywords: basal media; NAA; palm oil; plantlet; root inductionABSTRAKPerbanyakan klonal tanaman kelapa sawit menggunakan teknik kultur jaringan menghasilkan persentase tunas berakar yang rendah. Dalam upaya meningkatkan persentase tunas berakar yang lebih seragam maka dilakukan metode induksi akar yang didukung oleh penggunaan media dasar dan penambahan zat pengatur tumbuh 1-naphthaleneacetic acid (NAA). Penelitian ini bertujuan untuk menganalisis pengaruh kombinasi media dasar dan konsentrasi NAA yang optimum dalam menginduksi akar tunas kelapa sawit secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial yang terdiri dari 2 faktor. Faktor pertama adalah jenis media dasar yang terdiri dari media Murashige and Skoog (MS) dan MS Modifikasi (MSM). Faktor kedua adalah konsentrasi NAA yang terdiri dari 0; 0,05; 0,1; dan 0,2 ppm. setiap perlakuan diulang sebanyak 10 kali. Hasil penelitian menunjukkan bahwa penggunaan media MSM lebih baik daripada MS, dan konsentrasi NAA yang paling optimum adalah 0,05 dan 0, 1 ppm dalam menginduksi akar kelapa sawit secara in vitro. Selain itu kombinasi perlakuan MSM+NAA 0,1 ppm memiliki hasil yang paling optimum dalam induksi akar kelapa sawit secara in vitro.
SKRINING DAN ANALISIS FITOKIMIA TUMBUHAN OBAT TRADISIONAL MASYARAKAT KABUPATEN BIMA Nikman Azmin; Anita Rahmawati
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1113.415 KB) | DOI: 10.29122/jbbi.v6i2.3504

Abstract

Phytochemical Screening and Analysis of Traditional Herbal Medicines of Bima DistrictBima people use medicinal plants for traditional medicine which is local wisdom that must be preserved. Thus, the phytochemical content of these plants needs to be studied. This study aims to determine the secondary metabolite content of traditional medicinal plants in order to enrich biotechnology and pharmacological data. Thus, the data can be used as a reference in healing diseases and managing various medicinal plants based on community welfare and environmental sustainability for the people of Bima regency. The method used is a detailed interview and a field survey followed by phytochemical screening to determine the content of compounds in plants. From the results of this study, as many as 17 types of medicinal plants have been used as traditional medicine. The plant parts used are leaves, stems, flowers, roots, rhizomes, fruit, gum or lenders with 33% weed habitus followed by herbs (29%), trees (29%), and shrubs (9%). The compounds identified in these medicinal plants are flavonoids, alkaloids, steroids, terpenoids, saponins, and tannins.Keywords: Bima regency; local wisdom; medicinal plants; phytochemicals; secondary metabolitesABSTRAKMasyarakat Bima memanfaatkan tumbuhan obat untuk pengobatan tradisional yang merupakan kearifan lokal yang harus dipertahankan, sehingga kandungan fitokimia dari tumbuhan-tumbuhan ini perlu diteliti. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder tumbuhan obat tradisional guna memperkaya data bioteknologi dan farmakologi. Dengan demikian, data tersebut dapat dijadikan acuan dalam penyembuhan penyakit dan pengelolaan berbagai tumbuhan obat berbasis kesejahteraan masyarakat dan kelestarian lingkungan bagi masyarakat kabupaten Bima. Metode yang digunakan adalah wawancara secara rinci dan survei lapangan dilanjutkan dengan skrining fitokimia untuk mengetahui kandungan senyawa dalam tumbuhan. Dari hasil penelitian ini diketahui sebanyak 17 jenis tumbuhan obat yang telah dimanfaatkan sebagai obat tradisional. Bagian tumbuhan yang digunakan berupa daun, batang, bunga, akar, rimpang, buah, getah atau lender dengan jenis habitus 33% gulma diikuti oleh herbal (29%), pohon (29%), dan perdu (9%). Sedangkan senyawa yang teridentifikasi dalam tumbuhan obat tersebut yaitu flavonoid, alkaloid, steroid, terpenoid, saponin, dan tannin.
PENGARUH KONSENTRASI NANOPARTIKEL PERAK IONIK TERHADAP DAYA HAMBAT BAKTERI Lactobacillus casei DAN pH SUSU Vegisari Vegisari; Suparno Suparno; Tiara Delvika Rany
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.495 KB) | DOI: 10.29122/jbbi.v6i2.3506

Abstract

The Effect of Ionic Silver Nanoparticles Concentrations on the Inhibition of Lactobacillus casei and pH of MilkABSTRACTTechnological developments support the creation of various nanoparticles that can be utilized, one of which is silver nanoparticles. This study aims to determine the effect of ionic silver nanoparticle concentration on Lactobacillus casei growth inhibition, pH change and curdling of UHT milk. Variation in the concentration of ionic silver nanoparticles of 5, 10, 15, and 20 ppm was tested for their characteristics through UV-VIS spectrophotometry, showing the wave peaks at 413–430 nm, and through the PSA test resulting in the Z-average of 87.2 nm. Ionic silver nanoparticles were tested against L. casei using the Kirby-Bauer diffusion method for 72 hours. The test results showed a change in the clear zone that tended to increase for the concentration of silver nanoparticles 5, 10, 15 and 20 ppm with the gradient values of 0,0032; 0,0280; 0,0395; dan 0,0317, respectively. Tests for pH and UHT milk curdling were carried out for 78 hours and showed that ionic silver nanoparticles tended to be more able to maintain milk pH ±30 hours and curdling ±24 hours longer than milk that was not treated with ionic silver nanoparticles.Keywords: electrolysis; Lactobacillus casei; milk; nanoparticles; silver ABSTRAKPerkembangan teknologi mendukung terciptanya berbagai nanopartikel yang dapat dimanfaatkan, salah satunya nanopartikel perak. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi nanopartikel perak ionik terhadap daya hambat Lactobacillus casei, kadar pH, dan penggumpalan pada susu UHT. Variasi konsentrasi nanopartikel perak ionik 5, 10, 15, dan 20 ppm diuji karakteristiknya melalui spektrofotometri UV-VIS dengan hasil puncak gelombang serapan berada pada kisaran 413–430 nm dan uji PSA dengan hasil Z-average berada pada 87,2 nm. Sampel larutan nanopartikel perak ionik diujikan terhadap bakteri L. casei menggunakan metode difusi Kirby-Bauer selama 72 jam. Hasil uji menunjukkan terjadinya perubahan zona bening yang cenderung meningkat untuk konsentrasi nanopartikel perak 5, 10, 15 dan 20 ppm dengan masing-masing nilai gradiennya 0,0032; 0,0280; 0,0395 dan 0,0317. Pengaruhnya terhadap kadar pH dan penggumpalan susu UHT dilakukan selama 78 jam dan menunjukkan bahwa nanopartikel perak ionik cenderung lebih mampu mempertahankan pH susu ±30 jam dan penggumpalan ±24 jam lebih lama dibanding susu yang tidak diberikan perlakuan nanopartikel perak ionik.
EKSTRAKSI DAN IDENTIFIKASI METABOLIT SEKUNDER DARI ISOLAT AL6 SERTA POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli Alfian Syarifuddin; Sodiq Kamal; Fitriana Yuliastuti; Missya Putri Kurnia Pradani; Ni Made Ayu Nila Septianingrum
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2891.129 KB) | DOI: 10.29122/jbbi.v6i2.3516

Abstract

Extraction and Identification of Secondary Metabolites from AL6 Isolates and Its Potential as Antibacterial against Escherichia coliABSTRACTSecondary metabolites in the form of antibiotics can be produced by rhizospheric bacteria. AL6 bacterial isolate, which is one of the bacterial isolates from the rhosphere of Saccarum officinarum L., is known to produce antibiotic compounds. This study aims to determine the activity of antibiotics from AL6 ethyl acetate extracts produced by AL6 bacterial isolates, to analyze the minimum inhibitory concentration (MIC) and the similarity of the active substances using GCMS. The ethyl acetate extract obtained was tested for MIC at 1.25%, 2.5%, 5.0%, 10.0%, 20%, and 40% concentrations. Detection of potential antibiotic spots was carried out using bioautographic thin layer chromatography (TLC). Compounds responsible for antibiotic activity were analyzed using GCMS. Minimum inhibitory levels obtained reached 2.5%. The active spots responsible for antibiotic activity against Escherichia coli at Rf 0.94. Components detected using GCMS and suspected to be antibiotics include chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.Keywords: AL6 bacterial isolate; antibiotic; Escherichia coli; GCMS; MICABSTRAKMetabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.
KONSENTRASI MALONDIALDEHID PADA TIKUS DIABETIK YANG DIBERI PAKAN BERBAHAN SAGU (Metroxylon sagu) DAN Moringa oleifera Rahmah Nadea Fitriyani Muhajirin; A'immatul Fauziyah; Avliya Quratul Marjan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (129.861 KB) | DOI: 10.29122/jbbi.v6i2.3630

Abstract

Malondialdehyde Concentration in Diabetic Rats Given Feed Made from Sagu (Metroxylon sagu) and Moringa oleifera ABSTRACTHyperglycemia in type 2 diabetes mellitus (DMT2) can cause oxidative stress characterized by increased malondialdehyde (MDA) production. Flavonoids have antioxidant activity that can suppress the production of free radicals that cause oxidative stress. Flavonoids are found in Cersa mori (CM), a food product made from sagu (Metroxylon sagu) and Moringa oleifera. This study aims to analyze the effect of CM administration on MDA levels in alloxan-induced diabetic rats. Antioxidant activity and total CM flavonoids were analyzed using DPPH reduction and colorimetry methods. Thirty-two Wistar rats were divided into 4 groups: negative control (K1), positive control 1 (K2), positive control 2 (K3) and treatment of feeding 5 g CM/200 g BW per day (K4). The intervention was carried out for 30 days. MDA levels were examined before and after the intervention by spectrophotometry. The results showed a significant decrease in MDA levels in K2, K3, and K4 by -11.5 ± 3.39 μM, -10.5 ± 4.32 μM, and -14.9 ± 2.85 μM, respectively. The lowest decrease in MDA levels was found in K4 fed with CM (p < 0.05).Keywords: alloxan; diabetes; flavonoid; malondialdehyde; resistant starchABSTRAKHiperglikemia pada diabetes melitus tipe 2 (DMT2) dapat menimbulkan stres oksidatif yang ditandai dengan peningkatan produksi malondialdehid (MDA). Flavonoid memiliki aktivitas antioksidan yang mampu menekan produksi radikal bebas penyebab stres oksidatif. Flavonoid terdapat di dalam produk pangan Cersa mori (CM) yang terbuat dari sagu (Metroxylon sagu) dan kelor (Moringa oleifera). Penelitian ini bertujuan menganalisis pengaruh pemberian CM terhadap kadar MDA tikus diabetes yang diinduksi aloksan. Aktivitas antioksidan dan total flavonoid CM masing-masing dianalisis menggunakan metode reduksi DPPH dan kolorimetri.Tiga puluh dua ekor tikus Wistar dibagi menjadi 4 kelompok: kontrol negatif (K1), kontrol positif 1 (K2), kontrol positif 2 (K3), dan perlakuan yang diberikan 5 g CM/200 g BB per hari (K4). Intervensi dilaksanakan selama 30 hari. Kadar MDA diperiksa sebelum dan setelah intervensi secara spektrofotometri. Hasil menunjukkan adanya penurunan signifikan kadar MDA pada K2, K3 dan K4 yaitu secara berturut-turut sebesar -11,5 ± 3,39 µM; -10,5 ± 4,32 µM; dan -14,9 ± 2,85 µM. Penurunan kadar MDA terendah terdapat pada K4 yang diberikan CM (p < 0,05).
PENGARUH SEREAL BERBAHAN SAGU DAN Moringa oleifera TERHADAP KADAR GLUKOSA DARAH TIKUS YANG DIINDUKSI ALOKSAN Melin Novidinisa Herada Putri; A'immatul Fauziyah; Taufik Maryusman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.063 KB) | DOI: 10.29122/jbbi.v6i2.3669

Abstract

The Effects of Cereal Made From Sagu and Moringa oleifera on the Blood Glucose Level of Alloxan-Induced RatsDiabetes Mellitus (DM) type 2 could increase oxidative stress and blood glucose level. Resistant starch compounds in instant cereal Cersa Mori have antidiabetic properties. This research aimed to analyze the effect of Cersa Mori on fasting blood glucose (FBG) levels of diabetic white rats induced by alloxan. This is a true experimental study with a randomized pre-post control group design using 27 male Wistar strain rats divided into 3 groups randomly, i.e (KN) feed and distilled water, (KP) glibenclamide 0.126mg/200gBB/day, (P) Cersa Mori 5g/200gBB/day. KP and P groups were given alloxan 125 mg/KgBB subcutaneously and the intervention was carried out for 30 days. FBG level was measured using the GOD-PAP method. The results of Paired T-Test showed the effect of Cersa Mori on lowering FBG levels in hyperglycemic rats (P = 0,006). One-Way ANOVA test showed that Cersa Mori reduced FBG level, which was equivalent to those given glibenclamide (P = 0,366). It can be concluded that giving Cersa Mori 5g/200gBB/day for 30 days had a significant effect on lowering FBG level. Keywords: alloxan; Cersa Mori; diabetic rats; fasting blood glucose level; resistant strachABSTRAKDiabetes Mellitus (DM)tipe 2 dapat memicu stres oksidatif dan meningkatkan kadar glukosa darah puasa (GDP). Senyawa pati resisten dalam sereal siap saji Cersa Mori memiliki sifat antidiabetik. Penelitian ini bertujuan menganalisis pengaruh pemberian Cersa Mori terhadap kadar glukosa darah tikus putih diabetes yang diinduksi aloksan. Penelitian true-experimental ini menggunakan randomized pre-post control group design. Sampel sebanyak 27 ekor tikus jantan galur Wistar dibagi menjadi 3 kelompok secara acak yaitu; (KN) pakan dan akuades, (KP) glibenklamid 0,126 mg/200gBB/hari, (P) Cersa Mori 5g/200gBB/hari. KP sampai P diberikan aloksan 125 mg/KgBB secara subkutan dan intervensi dilakukan selama 30 hari. Pengukuran GDP menggunakan metode GOD-PAP. Hasil Uji-T menunjukkan pengaruh Cersa Mori dalam menurunkan GDP tikus hiperglikemia (P = 0,006). Uji ANOVA satu arah menunjukkan penurunan GDP pada kelompok Cersa Mori (P) setara dengan tikus yang diberi glibenklamid (P = 0,366). Dengan demikian dapat disimpulkan bahwa pemberian Cersa Mori dosis 5g/200gBB/hari selama 30 hari berpengaruh terhadap penurunan GDP secara signifikan.
RESPONS PERTUMBUHAN BIBIT KENTANG (Solanum tuberosum) TERHADAP FORMULASI BIOSTIMULAN BERBASIS Trichoderma spp. Winda Nawfetrias; Dwi Pangesti Handayani; Irna Surya Bidara; Armelia Tanjung
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.958 KB) | DOI: 10.29122/jbbi.v6i2.3710

Abstract

Growth Responses of Potato (Solanum tuberosum) Plantlets upon Application of Different Formulations of Trichoderma spp.-Based BiostimulantPlantlet acclimatization is one of several critical steps in potato clonal seedling production which often hampers the availability of quality plant materials. Application of biostimulant formulation containing Trichoderma spp. may increase growth capability of potato plantlets due to the improvement of plantlets in absorbing nutrient from the growth media. The aim of this research was to obtain the best formulations of biostimulant containing several strains of Trichoderma spp. to be applied in the acclimatization stage of potato seeds. Nine formulations of biostimulant (i.e. S1, S2, S3, S4, S5, S6, S7, S8, S9) each of which contained three to four different strains of five Trichoderma spp. isolates, were mixed with organic substrate carriers. The biostimulants were then applied to potato plantlets cv. Atlantic at the beginning of acclimatization. Plantlets without biostimulant were used as the control treatment (S0). The results showed that the S8 biostimulant formulation significantly increased the growth of the potato plantlets. The results of this experiment indicated that compared with the control and other formulations, the S8 biostimulant formulation significantly increased the growth of potato plantlets based on the number of leaves and number of shoots.Keywords: aclimatitation; biostimulant; potato; seedling; TrichodermaABSTRAKAklimatisasi planlet adalah salah satu tahapan kritis dalam produksi bibit klonal kentang yang seringkali menghambat ketercukupan bahan tanaman berkualitas. Penggunaan produk berbasis Trichoderma spp. diduga dapat meningkatkan kemampuan planlet untuk menyerap unsur hara dari media tumbuh. Tujuan penelitian ini adalah untuk mengetahui formulasi biostimulan terbaik yang mengandung Trichoderma spp. yang diaplikasikan pada tahap aklimatisasi bibit kentang. Sembilan biostimulan (S1, S2, S3, S4, S5, S6, S7, S8, S9) diformulasikan menggunakan substrat pembawa organik dan masing-masing mengandung campuran tiga atau empat strain dari lima isolat Trichoderma spp. Kesembilan biostimulan tersebut kemudian diujikan pada planlet kentang varietas Atlantik siap aklimatisasi. Planlet tanpa aplikasi biostimulan digunakan sebagai kontrol (S0). Hasil percobaan ini menunjukkan bahwa dibandingkan dengan control maupun formulasi lainnya, formulasi biostimulan S8 secara signifikan meningkatkan pertumbuhan planlet kentang cv. Atlantik dilihat dari jumlah daun dan jumlah tunasnya.

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