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EKSTRAKSI DAN IDENTIFIKASI METABOLIT SEKUNDER DARI ISOLAT AL6 SERTA POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli Alfian Syarifuddin; Sodiq Kamal; Fitriana Yuliastuti; Missya Putri Kurnia Pradani; Ni Made Ayu Nila Septianingrum
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2891.129 KB) | DOI: 10.29122/jbbi.v6i2.3516

Abstract

Extraction and Identification of Secondary Metabolites from AL6 Isolates and Its Potential as Antibacterial against Escherichia coliABSTRACTSecondary metabolites in the form of antibiotics can be produced by rhizospheric bacteria. AL6 bacterial isolate, which is one of the bacterial isolates from the rhosphere of Saccarum officinarum L., is known to produce antibiotic compounds. This study aims to determine the activity of antibiotics from AL6 ethyl acetate extracts produced by AL6 bacterial isolates, to analyze the minimum inhibitory concentration (MIC) and the similarity of the active substances using GCMS. The ethyl acetate extract obtained was tested for MIC at 1.25%, 2.5%, 5.0%, 10.0%, 20%, and 40% concentrations. Detection of potential antibiotic spots was carried out using bioautographic thin layer chromatography (TLC). Compounds responsible for antibiotic activity were analyzed using GCMS. Minimum inhibitory levels obtained reached 2.5%. The active spots responsible for antibiotic activity against Escherichia coli at Rf 0.94. Components detected using GCMS and suspected to be antibiotics include chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.Keywords: AL6 bacterial isolate; antibiotic; Escherichia coli; GCMS; MICABSTRAKMetabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.
Upaya Pencegahan Dampak Negatif Open Defecation Free (ODF) Di Dusun Sengon Desa Trasan Kecamatan Bandongan Kabupaten Magelang Nurul Hidayah; Sodiq Kamal; Atika Widiastuti; Muhammad Miftahul Syurur; Desti Mayawati; Fajar Nurrahmanto
JURNAL KREATIVITAS PENGABDIAN KEPADA MASYARAKAT (PKM) Volume 3 Nomor 2 Oktober 2020
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v3i2.2947

Abstract

Open Defecation Free (ODF) adalah salah satu kondisi suatu masyarakat telah melakukan sanitasi total yaitu dengan tidak Buang Air Besar Sembarangan (BABS). Suatu desa dapat dikatakan ODF jika 100% penduduk desa tersebut telah memiliki akses BAB di jamban sehat. Kurangnya pengetahuan tentang PHBS menimbulkan terjadinya perilaku ODF. Tujuan kegiatan Pengabdian Pada Masyarakat Terpadu (PPMT) ini untuk membantu memecahkan permasalahan masyarakat mengenai Open Defecation Free (ODF), dan memberikan alternatif dengan pembuatan water treatment yang dapat digunakan untuk membersihkan setelah Open Defecation Free (ODF) warga Dusun setempat. Pembuatan water treatment tersebut diharapkan dapat digunakan dengan baik oleh warga Dusun Sengon. Setelah adanya water treatment tersebut tim Pengabdian Pada Masyarakat Terpadu (PPMT) menguji air sebelum dan setelah adanya water treatment. Kandungan Coliform sebelum adanya water treatment sebanyak 1100 sedangkan setelah adanya water treatment menjadi 400. Terbukti bahwa water treatment dapat menurunkan kadar Coliform di dalam air tersebut. Kata Kunci: ODF, Open Defecation Free, Water Treatment  ABSTRACT Open Defecation Free (ODF) is one of the conditions in which a community has done total sanitation by not defecating openly (BABS). A village can be said to be ODF if 100% of the village population has access to defecating in a healthy latrine. Lack of knowledge about PHBS leads to ODF behavior. The purpose of this Integrated Community Service (PPMT) activity is to help solve community problems regarding ODF, and provide an alternative to making water treatment that can be used to clean up after ODF local Hamlet residents. The manufacture of water treatment is expected to be used properly by residents of Sengon Hamlet. After the water treatment, the PPMT team tested the water before and after the water treatment. The content of Coliform before the water treatment was 1100 while after the Water Treatment it became 400. It was proven that water treatment can reduce the levels of Coliform in the water. Keywords: ODF, Open Defecation Free, Water Treatment
Hospital Bed for Diabetes Care: An Invention to Support Professional and Hygienic Nursing Practice Sodiq Kamal; Suroto Munahar; Aries Abbas; Yoshifumi Ito; Agus Wahyudi
Mechanical Engineering for Society and Industry Vol 1 No 1 (2021)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.838 KB) | DOI: 10.31603/mesi.5262

Abstract

Appropriate diabetes mellitus (DM) wound care requires safe and comfortable space and facilities for patients and nurses. However, the existing hospital bed for DM has not supported the safety and comfort for nurses to serve patients, including the problem of liquid waste and some DM wounds emit a foul smell. Therefore, a hospital bed for DM wound care was designed in this research to support professional, efficient, ergonomic, and safe nursing practice. Multidisciplinary collaboration by engineers, wound nursing practitioners, and industry is carried out in this project. The level of risk of work disturbances was evaluated using a rapid entire body assessment (REBA), the level of risk of contamination was evaluated by a qualitative exposure assessment, and the level of comfort was measured using the visual analog method. Trials on 30 respondents consisting of 28 nurses and 2 doctors indicated that they were comfortable working with the new design of this prototype with lower risk.
Tingkat Kepuasan Pasien Terhadap Pelayanan Perawat Di Klinik Sembuh Lukaku Universitas Muhammadiyah Magelang Reczy Kurnia Vendy; Sodiq Kamal; Rohmayanti Rohmayanti
Prosiding University Research Colloquium Proceeding of The 15th University Research Colloquium 2022: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.073 KB)

Abstract

The abstract must be short, interesting, simple, and easy to understand without reading the entire article. Therefore, avoid jargon, abbreviations and references. In writing abstracts, the author must be accurate, use the right words, and convey the meaning of the study. A good abstract contains the problem statement and purpose, how the research is carried out (the method), the results, and concludes with a brief statement of conclusions. In the abstract keywords are also always included. Keywords are used to index an article and are the label of an article. Background: The level of satisfaction is a reflection of the quality of service. Patient satisfaction is the result of an assessment in the form of an emotional response to feelings of pleasure and satisfaction in patients because of the fulfillment of expectations or desires in using and receiving nurse services. Objective: This study aims to determine the level of patient satisfaction with the services of nurses at the Lukaku recovery clinic, Muhamadiyah University, Magelang. This study uses primary data sourced from direct observation and questionnaires distributed to respondents . Methods: This study used a questionnaire which was filled out by the respondents. Sampling used accidental sampling, namely the researcher took respondents at that time at the Lukaku Healing Clinic as many as 29 respondents. Results: The results of the data analysis revealed that the variables reliable, assurance, tangible, empathy, responsiveness affect the level of patient satisfaction with the services of nurses at the Healing Clinic. Lukaku
Care of Patient di Pelayanan Kesehatan Primer dalam Mendukung Kawasan Strategis Pariwisata Nasional Karima Millati; Sodiq Kamal; Sri Hananto Ponco Nugroho
Prosiding University Research Colloquium Proceeding of The 16th University Research Colloquium 2022: Mahasiswa (Student Paper Presentation)
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Pelayanan kesehatan primer dilakukan pada seluruh masyarakat untuk memastikan derajat kesehatan dan kesejahteraan dengan optimal yang berfokus pada rangkaian promotif, preventif, kuratif, maupun rehabilitatif.Joint Comission International (JCI) merupakan salah satu standar yang digunakan dalam ranah publik internasional dan siap digunakan oleh setiap organisasi pelayanan kesehatan dan badan umum dalam upaya meningkatkan perawatan pasien.Care of patient di pelayanan kesehatan primer menjadi salah satu ukuran kualitas pelayanan guna mendukung program Kawasan Strategis Pariwisata Nasional. Penelitian ini bertujuan untuk mengidentifikasi cakupan program care of patient pada pelayanan kesehatan primer di Borobudur dalam mendukung Kawasan Strategis Pariwisata Nasional mengacu pada standar Joint Comission International. Penelitian ini merupakan penelitian metode deskriptif dengan jumlah sampel 50 responden yang diambil dengan menggunakan teknik total sampling. Data dianalisis menggunakan analisis univariat.Sebagian besar tenaga kesehatan di Pelayanan Kesehatan Primer Borobudur dengan jumlah 42 responden (84%) dalam melakukan care of patient sudah berada di kategori partially met sesuai dengan standar JCI yang meliputi care deliver, high-risk services, resuscitation services, nutritional support, pain management, dan end-of-life care.
Breast milk production following Enhanced Recovery After Caesarean Section (ERACS) delivery: An evaluation study Tri Hastuti Budiningsih; Sodiq Kamal; Robiul Fitri Masitoh; Novi Kurniawan
Journal of Holistic Nursing Science Vol 10 No 1 (2023): January - June
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/nursing.v0i0.8421

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Sectio Caesarea delivery using the ERACS method is suspected of affecting milk production. Some researchers show results with varied parameters and inconsistent results. This study aims to determine the effect of various combinations of anesthetic drugs on breast milk production. This research is a descriptive correlation study with a cross-sectional approach with medical record documents as the data source. The sample in this study was 60 mothers who delivered by sectio caesarea at Aisyiyah Muntilan Hospital in July-October 2022. A total of 30 patients received a combination of anesthetic drugs 1 in topical and morphine, and 30 received a combination of anesthetic 2 in the form of vopicain, fentanyl, and dexmedetomidine. The results showed no difference in milk production in the patient group with combination 1 and 2 anesthetic drugs (p-value 0,000 with α: 0.05). This study concluded a difference in milk production in patients who received the anesthetic combination of vopicain, fentanyl, and dexmedetomidine and the combination of vopicain and morphine. Keywords: maternity care; ERACS; child Care; post partum care; mother and child health
Education for village ambulance drivers in patient evacuation Sodiq Kamal; Yeni Lusiyati; Judi Andriwanto; Tomy Sri Wardani Purnomo; Arifin Syam
Community Empowerment Vol 8 No 6 (2023)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.6887

Abstract

In order to support community health services, the Bandongan subdistrict government has provided each village with an ambulance unit. The available human resources, however, fall short in many ways. For this reason, the PPMT Unimma team is interested in educating village ambulance drivers so that their abilities can be improved. In cooperation with the Police Traffic Unit and Tidar Hospital, training and simulation are the method used to address this issue. As a result of this program, ambulance drivers now have more knowledge and expertise to evacuate the emergency patients.
Antidiabetic Activities of Broccoli Extracts (Brassica oleracea L.var italica) on Mice Induced Streptozotocin-Nicotinamide Heni Lutfiyati; Fitriana Yuliastuti; Imron Wahyu Hidayat; Prasojo Pribadi; Sodiq Kamal
Journal of Tropical Pharmacy and Chemistry Vol. 4 No. 4 (2018): J. Trop. Pharm. Chem.
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117, Gedung Administrasi Fakultas Farmasi Jl. Penajam, Kampus UNMUL Gunung Kelua, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jtpc.v4i3.203

Abstract

Several plant species have been used for the treatment of diabetes. Research on plants that can be used to treat diabetes such as Cinnamomum cassia and Cinnamomum zeylanicum, Piper sarmentosum Roxb, Nelumbo nucifera, Ceiba petandra Memecylon malabaricum. Broccoli (Brassica oleracea L var italics) is a flower of vegetables like cabbage plants. Broccoli juice is made capable of direct and indirect effects on the reduction of blood LDL; the indirect effect is by repairing pancreatic beta cells and increasing insulin sensitivity. The purpose of this study was to determine whether broccoli has activity as antidiabetic. The subjects consisted of 15 mice induced with nicotinamide 70 mg/kg BW and Stz 150 mg/kg BW. Diabetic mice were divided into the positive control group (glibenclamide), negative control group (CMC Na) and three treatment groups. The test material is broccoli extract dose 75 mg / kg BW, 150 mg /kg BW and 300 mg/kg BW given peroral for 14 days. Anova test results showed that there was a significant difference in the average percentage of decreased blood glucose levels in all treatments. The result of Post Hock Tukey HSD test with a 95% confidence level showed a significant difference in the negative control group compared with positive control group and extract 300mg / kg BW. This shows that positive control group and extracts 300mg / kg body weight have an activity to decrease blood glucose level.