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All Journal Jurnal Kesehatan Jurnal Bioteknologi & Biosains Indonesia (JBBI) JPM (Jurnal Pemberdayaan Masyarakat) Jurnal Pengabdian Pada Masyarakat Jurnal Ilmu Kefarmasian Indonesia Jurnal Pengabdian Masyarakat Karya Husada (JPMKH) Community Empowerment Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Farmasi Sains dan Praktis Urecol Journal. Part D: Applied Sciences Prosiding University Research Colloquium Jurnal Farmasi Klinik dan Sains Borobudur Pharmacy Review

Alfian Syarifuddin

Universitas Muhammadiyah Magelang, Indonesia

Author-ID : 3684182

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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EKSTRAKSI DAN IDENTIFIKASI METABOLIT SEKUNDER DARI ISOLAT AL6 SERTA POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli Alfian Syarifuddin; Sodiq Kamal; Fitriana Yuliastuti; Missya Putri Kurnia Pradani; Ni Made Ayu Nila Septianingrum
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Extraction and Identification of Secondary Metabolites from AL6 IsolatesÂ and Its Potential as Antibacterial against Escherichia coliABSTRACTSecondary metabolites in the form of antibiotics can be produced by rhizospheric bacteria. AL6 bacterial isolate, which is one of the bacterial isolates from the rhosphere of Saccarum officinarum L., is known to produce antibiotic compounds. This study aims to determine the activity of antibiotics from AL6 ethyl acetate extracts produced by AL6 bacterial isolates, to analyze the minimum inhibitory concentration (MIC) and the similarity of the active substances using GCMS. The ethyl acetate extract obtained was tested for MIC at 1.25%, 2.5%, 5.0%, 10.0%, 20%, and 40% concentrations. Detection of potential antibiotic spots was carried out using bioautographic thin layer chromatography (TLC). Compounds responsible for antibiotic activity were analyzed using GCMS. Minimum inhibitory levels obtained reached 2.5%. The active spots responsible for antibiotic activity against Escherichia coli at Rf 0.94. Components detected using GCMS and suspected to be antibiotics include chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.Keywords: AL6 bacterial isolate; antibiotic; Escherichia coli; GCMS; MICABSTRAKMetabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.

POTENSI CEMARAN BAKTERI Escherichia coli PADA LIMBAH CAIR RUMAH POTONG AYAM (RPA) TERHADAP LINGKUNGAN DI KOTA MAGELANG Alfian Syarifuddin; Fitriana Yuliastuti; Missya Putri Kurnia Pradani
Jurnal Kesehatan Vol 13, No 1 (2020): Jurnal Kesehatan
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/jk.v13i1.11101

Kualitas air secara biologis ditentukan oleh banyak parameter, antara lain parameter mikroba pencemar/ patogen. Bakteri Escherichia coli merupakan salah satu bakteri yang menjadi salah satu parameter kualitas suatu air. Jumlah kasus diare di Kota Magelang tahun 2014 mengalami kenaikan dibanding kasus diare tahun 2013, yaitu sebanyak 3.577 kasus dengan persentase sebesar 139,59% yang disebabkan oleh bakteri. Penelitian ini dilakukan untuk mengetahui ada tidaknya cemaran bakteri Escherichia coli dan mengetahui nilai Angka Lempeng Total (ALT) cemaran bakteri Escherichia coli pada air bilasan pada Rumah Potong Ayam (RPA) yang dapat berpotensi sebagai pencemar di lingkungan Kota Magelang. Metode yang digunakan dalam penelitian ini adalah cawar sebar dengan menggunakan media untuk pengujian adalah media TBX agar. Hasil penelitian menunjukkan adanya cemaran bakteri Escherichia coli yang melebihi 1x101CFU/ml pada air bilasan ayam pada beberapa RPA di Kota Magelang, yaitu 61,1% sampel uji atau sekitar 11 tempat sampling dari total sebanyak 18 tempat pemotongan ayam. Hasil tersebut menunjukkan bahwa air hasil bilasan tersebut dapat berpotensi sebagai salah satu sumber cemaran bakteri Escherichia coli pada daging ayam dan air tanah lingkungan sekitar pembuangan limbah air bilasan. Hanya terdapat 38,9% dari 18 RPA masih memenuhi ambang batas cemaran bakteri Escherichia coli. Oleh karena itu, perlu adanya langkah-langkah strategis berikutnya, yaitu pengolahan air limbah RPA dengan proses biofilter, serta dapat disosialisasikan terkait cara mengolah daging yang benar.

KARAKTERISASI FRAKSI TERAKTIF SENYAWA ANTIBIOTIK ISOLAT KP 13 DENGAN METODE DENSITOMETRI DAN KLT-SEMPROT Alfian Syarifuddin; Nanik Sulistyani
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 4 No 1 (2019): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Exploration of microorganisms Actinomycetes can produce antibiotic compounds. One of the bacteria that can produce antibiotic compounds is the KP13 bacterial isolate. The purpose of this study was to determine the character of the fraction of KP13 isolates using the densitometry method and the spray / colouring reaction of thin layer chromatography elution. The wavelengths used for densitometry 210 nm and spray reagents used include ninhydrin, Dragendorff, 2,4-dinitrophenyl hydrazine (2,4-DNPH), and sulfuric acid anisaldehyde. The results of the study using densitometry at a wavelength of 210 nm showed the highest peak chromatogram at Rf 0.78. The compound that is thought to be responsible for the Rf value of 0.78 has the characteristics of Terpenoid compounds, alkaloids, and carbonyl.

STUDI ETNOMEDISIN PADA MASYARAKAT 5 DESA KECAMATAN SECANG KABUPATEN MAGELANG Alfian Syarifuddin; Rizka Amalia
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 6 No 2 (2021): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

The use of plants for traditional medicines are community tradition in Indonesia. However, the use of plants for hereditary treatment has not been well documented. One of the solutions is an ethnomedicine study, in this study 5 villages located in Secang District, Magelang Regency. The sampling technique used is snowball sampling with observation using instruments and interviews. The number of respondents sample is 388 respondents. The results showed that the community utilized 96 plant species from 47 families. There are 3 types of diseases that use plants in their treatment with an ICF value of 1, namely sprains, nosebleeds, and menstrual flow. The most widely used parts for treatment are leaves (42.61%). The most frequently used processing method is the boiling technique with a percentage of 65.10% by way of use, which is drinking 77.05%. People most often consume with a frequency of 2x a day (74.87%). People usually use treatment during illness/ curative (76.74%). People get plants from their own yard (54.24%). Knowledge about treatment comes from family (hereditary) (72.43%). From some of the results, it can be used as a reference when conducting scientific verification of preclinical testing on test animals.

Aktivitas Antibiotik Isolat Bakteri Kp13 dan Analisa Kebocoran Sel Bakteri Escherichia coli Alfian Syarifuddin; Nanik Sulistyani; Kintoko Kintoko
JURNAL ILMU KEFARMASIAN INDONESIA Vol 16 No 2 (2018): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

A bacterium is one of microorganisms that can produce secondary metabolite, such as antibiotic. KP13 isolate is a bacterium isolated from Melaleucaleuca dendron L rhizosphere. The aim of this study is to know the eff ect of the most active fraction antibacterium of KP13 isolate toward Escherichia coli bacterium and the ability to caused cell leakage. Extraction of antibiotic was conducted by ethyl acetate. Extract is fractioned with a n-heksan-etilacetate solvent gradient another uses a solvent methanol. Each fraction is done by KLT and grouped based on RF value. A fraction group is compare by inhibition eff ectiveness. Then, the Minimum Inhibitory Concentration (MIC) from the most active group is tested. Cell leakage analysis was performed using UV spectrophotometry to detect the release of nucleic acid and protein. Atomic Absorption Spectrophotometry (AAS) was used to detect ion release of K+ and Ca2+ from. cell of bacteria. The results showed that the most active fraction against Escherichia coli was the fraction number 1 with MIC value of 5% and inhibition zone of 7.33±0.58 mm.

Kegiatan Literasi pada Ibu PKK Dusun Bendan Sawangan Magelang dalam Rangka Meningkatkan Penggunaan Obat secara Rasional Widarika Santi Hapsari; Fitriana Yuliastuti; Alfian Syarifuddin; Ni Made Ayu Nila Septianingrum
Jurnal Pemberdayaan Masyarakat Vol 5 No 2 (2020): November
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jpm.v5i2.3642

Self-medication activities are part of the community's efforts to take care of their health. Gevaarlijk and antibiotics are types of drugs that can only be obtained by prescription from a doctor. Both types of these drugs should not be stored because their use must be under the supervision and has the potential to cause bacterial resistance related to the use of antibiotics. Based on Riskesdas 2013, there are still many people who store gevaarlijk and antibiotics for self-medication. This shows the irrational use of drugs. Community service activities aim to improve knowledge and skills in the use of rational medicine. The method used is the Community-Based Interactive Approach method in which participants play an active role in activities. This participation is important to practice the ability and skill in solving problems. Activities carried out through counseling about the use of rational drugs and antibiotics, then continued with training and assistance for the rational use of drugs and antibiotics. End result of this activity is expected that the community will be able to apply rational drug use and disseminate this information to the wider community.

Peningkatan Imunitas Keluarga dan Budaya Kewirausahaan Masa Pandemi Covid-19 melalui Budidaya Toga di Desa Tempursari, Candimulyo, Magelang Retno Rusdidjati; Alfian Syarifuddin; Oesman Raliby; Agus Suprapto
Jurnal Pengabdian Pada Masyarakat Vol 6 No 1 (2021)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202161.560

INCREASING FAMILY IMMUNITY AND ENTREPRENEURSHIP CULTURE DURING THE COVID-19 PANDEMIC THROUGH TOGA CULTIVATION IN TEMPURSARI VILLAGE, CANDIMULYO, MAGELANG. Regional economic development is the process of local government and society in managing potential local commodity resources. Development programs are not only seen from an economic or physical perspective, but also in the health sector. This pandemic condition needs developments in the health sector. Tempursari Village, Candimulyo District, Magelang Regency has a very good potential to develop family medicinal plants. The Program Kemitraan Wilayah (PKW) team from the University of Muhammadiyah Magelang and Untidar empowered the people of Tempursari Village to cultivate medicinal plants on demonstration plot lands owned by village treasury lands. Cultivation or planting that is done is planting pepper, lime and lemongrass. The purpose of planting is so that these plants can be used as an immune booster. The harvest results from these medicinal plants can later be processed into a preparation so that a new business unit appears in Tempursari Village, Candimulyo District, Magelang Regency.

PKU BAGI MASYARAKAT DI DESA CONGKRANG, KECAMATAN MUNTILAN, KABUPATEN MAGELANG, JAWA TENGAH Imron Wahyu Hidayat; Metty Azalea; Alfian Syarifuddin
Jurnal Pengabdian Masyarakat Karya Husada (JPMKH) Vol 4 No 1 (2022): Jurnal Pengabdian Masyarakat Karya Husada (JPMKH)
Publisher : LPPM Politeknik Kesehatan Karya Husada Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Jamu merupakan ramuan obat tradisional yang diwariskan secara turun temurun. Mengembangkan dan melestarikan jamu penting untuk dilakukan selain memberikan manfaat kesehatan, meningkatakan perekonomian UKM juga turut serta melestarikan budaya Masyarakat Desa Congkrang, Kecamatan Muntilan, Kabupaten Magelang mayoritas mata pencahariannya adalah petani dan penjual jamu gendong. Mitra dalam pengabdian ini adalah Penjual jamu Gendong Anggota Paguyuban KBU Tirta sari Desa Congkrang. Metode yang digunakan adalah model pemberdayaan masyarakat partisipatif Participatory Rural Apraisal (PRA). Tahap pelaksanaan kegiatan meliputi : 1) Sosialisasi tentang pembuatan jamu gendong yang higienis dan terstandar, 2) pelaksanaan meliputi sosialisasi tentang kegiatan pengabdian kepada masyarakat kepada mitra dan perangkat desa, Achieve Motivation Training, penguatan kelembagaan paguyuban, standarisasi proses pembuatan jamu, pendampingan.Setelah berpartisipasi dalam kegiatan ini, masyarakat mengalami peningkatan pengetahuan mengenai pembuatan jamu gendong terstandar, higienis dan memiliki kualitas seragam.

Increasing the role of women's farmer groups in Tempursari Village, Candimulyo, Magelang, through a culinary business based on banana and taro processing Retno Rusdjijati; Alfian Syarifuddin; Oesman Raliby Al Manan; Agus Suprapto
Community Empowerment Vol 6 No 11 (2021)
Publisher : Universitas Muhammadiyah Magelang

Banana and taro are two of the many plants that grow in Tempursari Village. These commodities will have a low selling value if traded directly. As a result, a processed food diversification strategy is required to increase the economic value of these plants, such as processed chips with flavor variants based on the intended target market. The mentoring activity was carried out with the participation of 20 participants, who were representatives from two hamlets. Outreach activities, training, practice, and mentoring are all part of the implementation process. Based on these activities, the outcomes of processed food products, particularly banana chips and taro chips, have the potential to provide an alternative method of increasing the economy by utilizing abundant natural resources.

PROFIL KLT-BIOAUTOGRAFI DAN DENSITOMETRI SENYAWA TERAKTIF (ISOLAT KP13) DARI RIZOSFER KAYU PUTIH Alfian Syarifuddin; Nanik Sulistyani; Kintoko -
Jurnal Farmasi Sains dan Praktis Vol 5 No 1 (2019)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/pharmacy.v5i1.2291

The rate of infection, especially in Indonesia is still high. Treatment of infections by using antibiotics is constrained by cases of resistance to antibiotics so that it is necessary to explore microorganisms that can produce antibiotics. Antibiotics produced by KP13 bacterial isolates have the potential as antibiotics against Escherichia coli. The purpose of this study was to determine the TLC-Bioautography profile and Densitometry analysis of the most active fraction of KP13 bacterial isolates which have the potential as antibiotics against Escherichia coli bacteria. TLC using the mobile phase Chloroform: Ethylacetate: Methanol (4: 1: 0.5). Active compounds were analyzed using GC-MS. The results showed active spots during TLC-Biaoautography with a Rf value of 0.78. Compounds that are thought to be responsible for antibiotic activity detected by GC-MS at a Rf value of 0.78 have similarities with the compounds of cycloheptatriene and tetrahydrophyran..

Co-Authors agus suprapto Amalia Ratna Puspitadewi Enny Siti Isnaeni Fitriana Yuliastuti Fitriana Yuliastuti Herma Fanani Agusta Imron Wahyu Hidayat Imron Wahyu Hidayat Imron Wahyu Hidayat Kintoko - Kintoko, Kintoko Metty Azalea Missya Putri Kurnia Pradani Missya Putri Kurnia Pradani Missya Putri Kurnia Pradani Misya Putri Kurnia Pradani Nanik Sulistyani Nanik Sulistyani Nanik Sulistyani Ni Made Ayu Nila Septianingrum Ni Made Ayu Nila Septianingrum Ni Made Ayu Nila Septianingrum Ni Made Ayu Nila Septianingrum Nufikha Falyauma Oesman Raliby Oesman Raliby Al Manan Puspita Septie Dianita Ratna Wijayatri Retno Rusdidjati Rizka Amalia Rusdjijati, Retno Sodiq Kamal Sodiq Kamal Sutiara Prihatining Tyas Widarika Santi Hapsari Zainab - Zikri Almahdi Zulda Sarah Kusumawati

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