cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta pusat,
Dki jakarta
INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
 28   29   30   31   32 
PENGARUH SINBIOTIK KEFIR PISANG BATU TERHADAP KADAR KOLESTEROL-LDL DAN KOLESTEROL-HDL TIKUS MODEL SINDROM METABOLIK Dina Khoiriyah; Taufik Maryusman; Santi Herlina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i2.3781

Abstract

Effect of Banana Kefir Synbiotic on LDL-Cholesterol and HDL-Cholesterol of Metabolic Syndrome Rats Metabolic syndrome (SM) is characterized by several risk factors including dyslipidemia. This study aims to analyze the effect of kefir synbiotic produced from banana stone flour (Musa balbisiana) on LDL-cholesterol and HDL-cholesterol of metabolic syndrome rat model. The 24 Sprague Dawley rats were divided into four groups, namely negative control (healthy rats fed standard feed), positive control (metabolic syndrome rats fed standard feed), treatment I and treatment II (metabolic syndrome rats each given synbiotic kefir banana stone flour 1.8 mL/200 g mouse BW/day and 3.6 mL/200 g mouse BW/day, respectively). The intervention was carried out for three weeks. After the intervention, the levels of LDL-cholesterol in treatment I and II experienced a decrease of 44.66% and 56.94%, respectively, while the-HDL-cholesterol levels in treatment I and II experienced an increase of 104.5% and 172.71%, respectively. The biggest change occurred in treatment II. Synbiotic kefir banana stone flour improved lipid profile in metabolic syndrome rats. Sindrom metabolik (SM) ditandai dengan beberapa faktor risiko termasuk dislipidemia. Penelitian ini bertujuan untuk menganalisis pengaruh sinbiotik kefir tepung pisang batu (Musa balbisiana) terhadap kadar kolesterol-LDL dan kolesterol-HDL tikus model SM. Subjek menggunakan 24 ekor tikus Sprague Dawley yang dibagi menjadi empat kelompok, yaitu kontrol negatif (tikus sehat yang diberi pakan standar), kontrol positif (tikus model SM yang diberi pakan standar), perlakuan I dan perlakuan II (tikus model SM yang masing-masing diberi sinbiotik kefir tepung pisang batu 1,8 mL/200 g BB tikus/hari dan 3,6 mL/200 g BB tikus/hari). Proses intervensi dilakukan selama tiga minggu. Setelah intervensi, kadar kolesterol-LDL perlakuan I dan II mengalami penurunan sebesar 44,66% dan 56,94%, sedangkan kadar kolesterol-HDL perlakuan I dan II mengalami peningkatan sebesar 104,5% dan 172,71%. Perubahan terbesar terjadi pada perlakuan II. Sinbiotik kefir tepung pisang batu memperbaiki profil lipid tikus sindrom metabolik.
EKPRESI ANTIGEN Ag85B DARI Mycobacterium tuberculosis PADA GALUR SEL MAMALIA Sabar Pambudi; Tika Widayanti; Nadya Stephanie
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.863 KB) | DOI: 10.29122/jbbi.v7i1.3840

Abstract

Expression of Mycobacterium tuberculosis Ag85B Antigen in Mammalian Cell CultureTuberculosis (TB) continues to be a major health problem worldwide, affecting millions of people each year. The only vaccine approved for the prevention of TB is Bacillus Calmette-Guérin (BCG). However, one of the limitations of BCG is that its preventive effect against pulmonary TB varies from person to person. Therefore, there arises a need for a new TB vaccine to replace BCG. This study aims to obtain the Ag85B recombinant protein which has characteristics similar to the native Ag85B antigen from Mycobacterium tuberculosis. In this study, we cloned and expressed recombinant Ag85B in mammalian cell culture. In the initial step, we cloned synthetic Ag85B into mammalian expression vector pFLAG-CMV4 and expressed the gene in CHO-K1 cells. Interestingly, a specific band around 30 kDa was observed in the culture media of transfected cells by Western blot analysis. The results from our research showed the potency of mammalian expression system to produce recombinant protein Ag85B for new TB vaccine candidate.Keywords: Ag85B, mammalian cells, tuberculosis, vaccine, expression ABSTRAKTuberkulosis (TB) terus menjadi salah satu masalah kesehatan dunia yang mempengaruhi jutaan manusia setiap tahun. Satu-satunya vaksin untuk TB yang ada adalah Bacillus Calmette-Guérin (BCG). Namun demikian, vaksin BCG ini memiliki kelemahan berupa terjadi efek preventif yang bervariasi dari satu individu terhadap individu lainnya.  Oleh sebab itu diperlukan pengembangan vaksin TB yang dapat menggantikan vaksin BCG yang sudah ada. Penelitian ini bertujuan memperoleh protein rekombinan Ag85B yang memiliki karakteristik mirip dengan antigen Ag85B native dari Mycobacterium tuberculosis. Pada penelitian ini, telah dilakukan kegiatan pengklonaan dan ekspresi gen Ag85B pada galur sel mamalia.  Pada tahap awal dilakukan pengklonaan gen sintesis Ag85B ke dalam plasmid pada sel mamalia pFLAG-CMV4 dan diekspresikan gennya pada sel CHO-K1. Hasil analisis Western blot menunjukan tersekresinya gen target berukuran 30 kDa pada media kultur dari sel mamalia yang ditransfeksi. Hasil dari penelitian ini menunjukkan potensi dari sistem ekpresi untuk protein rekombinan Ag85B pada galur sel mamalia sebagai kandidat vaksin TB yang baru.
FITOREMEDIASI AIR LIMBAH LABORATORIUM ANALITIK UNIVERSITAS JEMBER DENGAN PEMANFAATAN TANAMAN ECENG GONDOK DAN LEMBANG Elida Novita; Sri Wahyuningsih; Dwi Andriana Na'imatul Jannah; Hendra Andiananta Pradana
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1467.991 KB) | DOI: 10.29122/jbbi.v7i1.3850

Abstract

Phytoremediation of Analytical Laboratory of Jember University Waste Water by The Use of Water Hyacinth and Cattail PlantsAnalytical laboratory waste water at Jember University has organic and inorganic materials which can be categorized as biodegradable or non-biodegradable wastes. This study focused on comparing the ability between water hyacinth (Eichhornia crassipes) and cattail (Typha angustifolia) plant in reducing the pollutants as a consideration in selecting plants for waste water treatment at Jember University. The stages in this research consisted of filtration using silica sand, adsorption using activated carbon and zeolites, and phytoremediation using water hyacinth and cattail plants. The phytoremediation treatment was carried out during 14 days with a density of 40 g L–1. Cattail plant treatment had a higher value of pollutant reduction efficiency in waste water compared to water hyacinth. The reduction efficiency parameters. namely turbidity, TSS, BOD, COD, and Cr, were 92.18, 84, 74, 64, and 49%, respectively. The results of this study provide an alternative treatment for laboratory waste water which has an environmentally friendly character at Jember University.Keywords: Chromium (Cr), Eichhornia crassipes, filtration and adsorption, Typha angustifolia, water qualityABSTRAKAir limbah laboratorium analitik di Universitas Jember mengandung bahan organik dan anorganik yang bersifat mudah diuraikan maupun toksik. Tujuan penelitian ini adalah membandingkan kemampuan reduksi polutan oleh eceng gondok (Eichhornia crassipes) dan lembang (Typha angustifolia) sebagai pertimbangan pemilihan tanaman untuk menangani air limbah laboratorium di lingkungan Universitas Jember. Tahapan penelitian terdiri atas filtrasi menggunakan pasir silika, adsorpsi menggunakan karbon aktif dan zeolit, serta fitoremediasi menggunakan eceng gondok dan lembang. Waktu tanaman eceng gondok dan lembang diinkubasi menggunakan teknik fitoremediasi selama 14 hari dengan densitas 40 g L–1. Hasil penelitian menunjukkan bahwa penanganan air limbah menggunakan lembang memiliki nilai efisiensi reduksi polutan pada air limbah lebih tinggi daripada eceng gondok. Nilai efisiensi reduksi tersebut berupa parameter kekeruhan, TSS, BOD, COD, dan Cr secara berurutan sebesar 92,18, 84, 74, 64, dan 49%. Hasil penelitian ini menjadi alternatif penanganan air limbah laboratorium yang ramah lingkungan.
UJI KEMAMPUAN DAYA SERAP HANJUANG (Cordyline fruticosa) SEBAGAI AGEN FITOREMEDIASI LOGAM Pb PADA MEDIA TANAH Nelis Hernahadini; Luthfia Hastiani Muharram; Noviani Arifina Istiqomah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.462 KB) | DOI: 10.29122/jbbi.v7i1.3859

Abstract

Absorption Capability Test of Hanjuang (Cordyline fruticosa) as Fitoremediation Agent of Lead in Soil MediumLead is a heavy metal waste that is dangerous for the environment and health. The use of ornamental plants is an alternative in reducing heavy metal pollution. Hanjuang (Cordyline fruticosa) is an ornamental-plant phytoremediation agent that can absorb heavy metals especially lead (Pb) at a high concentration. This study aims to test the Pb absorption ability of Hanjuang plant. Hanjuang was planted in a medium containing Pb at the concentration of 50 mg kg–1 with variable planting time of 4, 6, 8, 10, and 12 weeks. The measurement of Hanjuang's absorption of Pb was carried out on the roots, stems, and leaves by the atomic absorption spectroscopy (AAS) method. The results showed that the highest absorption capacity of 63.4% occurred at 12 days planting time. Whereas the amount of Pb accumulation in each part of the plant, from the highest to the lowest concentration, was found in the roots, leaves, and stems, consecutively. The ability of the plant's absorption of Pb was reduced with increasing metal concentrations in the media.Keywords: Hanjuang, heavy metal, lead, phytoremediation, solid wasteABSTRAKTimbal menjadi salah satu limbah logam berat yang berbahaya untuk lingkungan dan kesehatan. Penggunaan tanaman hias menjadi alternatif dalam mengurangi pencemaran logam berat. Hanjuang (Cordyline fruticosa) merupakan tanaman hias agen fitoremediasi yang memiliki kemampuan menyerap logam berat khususnya timbal (Pb) yang cukup tinggi. Penelitian ini bertujuan melakukan uji daya serap tanaman hanjuang terhadap logam Pb. Hanjuang ditanam pada medium tanah berlogam Pb dengan kadar 50 mg kg–1 dengan variabel waktu penanaman selama 4, 6, 8, 10 dan 12 minggu. Pengukuran daya serap Hanjuang terhadap Pb dilakukan pada bagian akar, batang, dan daun dengan metode spektroskopi serapan atom (AAS). Hasil menunjukkan bahwa daya serap tertinggi terjadi pada waktu tanam 12 hari dengan kadar 63,4%. Sedangkan jumlah akumulasi tiap bagian tanaman paling tinggi ke rendah terdapat pada bagian akar, daun, dan batang. Pada variasi konsentrasi, kemampuan daya serap tanaman terhadap Pb berkurang seiring meningkatnya konsentrasi logam pada media.
PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK KASAR RESIN DAN DAUN GAHARU (Gyrinops versteegii) I Gde Adi Suryawan Wangiyana; . Akram; Farouq Isbulloh
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.901 KB) | DOI: 10.29122/jbbi.v7i1.3862

Abstract

Comparison of Antibacterial Activities of Agarwood (Gyrinops versteegii) Resin and Leaf Crude ExtractThis research aim is to compare antibacterial activities of leaves extract and resin extract of Gyrinops versteegii with different extraction solvent and concentration. Leaves and resin had been prepared by drying and grinding then were extracted by maceration method. Factorial experiment design was used for extract application to Staphylococcus aureus ATCC25923. First factor was extract ingredient, second factor was extraction solvent, third factor was extract concentration. Inhibition zone as main parameter for antibacterial assay were analysed by ANOVA, HSD test and standard error. The inhibition zone of resin was higher than inhibition zone of leaves. The extraction solvent and extract concentration were not significantly resulted in different inhibition zone diameter. However, there were unique interaction between extraction solvent and extraction concentration that affected inhibition zone diameter. It could be concluded that the inhibition zone of resin was higher than that of leaves while no significant result from extraction solvent and extract concentration factorsKeywords: antibacterial, agarwood, extract, leaves, resin ABSTRAKPenelitian ini bertujuan untuk membandingkan aktivitas antibakteri daun gaharu dan resin gaharu G. versteegii menggunakan beberapa pelarut pengekstrak dan konsentrasi ekstrak yang berbeda. Bahan daun dan resin gaharu dikeringkan dan dicacah, kemudian diekstrak menggunakan metode maserasi. Uji aplikasi ekstrak pada Staphylococcus aureus ATCC25923 dilakukan menggunakan rancangan faktorial, yaitu faktor pertama bahan ekstrak (resin, daun), faktor kedua pelarut pengekstrak (etanol, metanol), dan faktor ketiga konsentrasi ekstrak (0,25; 0,5; dan 1 g mL–1). Data zona hambat terhadap bakteri uji dianalisis menggunakan ANOVA, BNJ dan standard error. Rerata zona hambat ekstrak daun gaharu (7 mm) lebih besar secara signifikan dibandingkan dengan rerata zona hambat ekstrak resin (6,5 mm). Faktor pelarut pengekstrak dan konsentrasi ekstrak tidak berpengaruh signifikan terhadap zona hambat. Pelarut pengekstrak dan konsentrasi ekstrak memberikan pengaruh interaksi berbeda-beda terhadap zona hambat. Dapat disimpulkan bahwa bahan ekstrak daun gaharu memiliki aktivitas antibakteri lebih bagus dibandingkan dengan ekstrak resin gaharu dengan konsentrasi ekstrak efisien sebesar 0,25 g mL–1.
ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT DARI PANGAN FERMENTASI CINCALOK SEBAGAI PENGHASIL GAMMA-AMINOBUTYRIC ACID Adhitya Naufal Pribadhi; Endang Kusdiyantini; Rejeki Siti Ferniah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.96 KB) | DOI: 10.29122/jbbi.v8i1.3906

Abstract

Isolation and Characterization of Lactic Acid Bacteria from Fermented Food Cincalok as Producer of Gamma-Aminobutyric Acid Cincalok is a fermented food originating from West Kalimantan. This study aimed to obtain lactic acid bacterial isolates (LAB) capable of producing gamma-aminobutyric acid (GABA), to characterize the LAB isolates obtained, and to obtain GABA by the Thin Layer Chromatography (TLC) method. Bacterial growth and GABA production was carried out by adding 5% MSG and without MSG, and measured spectrophotometrically. In this study, 4 LAB bacterial isolates were obtained which were coded CIN-1, CIN-2, CIN-3, and CIN-4. GABA identification of all the LAB isolates using TLC Silica Gel 60 F254 with butanol: acetic acid: distilled water (5: 3: 2) as eluent yielded Rf 0.61 and Rf MSG 0.38. The highest growth was achieved by isolate CIN-3 with an absorbance of 1.488 (at 48 hour) in non-MSG medium, while the addition of 5% MSG resulted in an absorbance of 1.631 (at 42 hour). GABA production was achieved by isolate CIN-3 with 5% MSG treatment with a concentration of 201.472 mM and without MSG with a concentration of 171.195 mM. Cincalok merupakan pangan fermentasi yang berasal dari Kalimantan Barat. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat (BAL) yang mampu menghasilkan gamma-aminobutyric acid (GABA), melakukan karakterisasi isolat BAL yang diperoleh dan dapat diperoleh GABA dengan metode Kromatografi Lapis Tipis (KLT). Penumbuhan bakteri dan produksi GABA dilakukan dengan penambahan MSG 5% dan tanpa MSG, dan diukur menggunakan spektrofotometer. Dalam penelitian ini diperoleh 4 isolat bakteri BAL yang diberi kode CIN-1, CIN-2, CIN-3, dan CIN-4. Identifikasi GABA dari semua isolat BAL tersebut menggunakan KLT Silica Gel 60 F254 dengan eluen butanol: asam asetat: aquades (5: 3: 2), menghasilkan Rf 0,61 dan Rf MSG 0,38. Pertumbuhan tertinggi terjadi pada isolat CIN-3 non MSG dengan absorbansi 1,488 (jam ke-48), sedangkan dengan penambahan MSG 5% menghasilkan absorbansi 1,631 (jam ke-42). Produksi GABA dicapai isolat CIN-3 dengan perlakuan MSG 5% dengan konsentrasi 201.472 mM dan tanpa MSG dengan konsentrasi 171,195 mM.
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Yuna Annisa Salsabila; Enny Suswati; Bagus Hermansyah; Dini Agustina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
PURIFIKASI PROTEIN IMUNOGENIK 31 DAN 56 kDa DARI KELENJAR SALIVA Aedes aegypti Syubbanul Wathon; Fitria Mutiah; Rike Oktarianti; Kartika Senjarini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3931

Abstract

Purification of 31 and 56 kDa Immunogenic Proteins from the Salivary Glands of Aedes aegyptiThe salivary gland of arthropod vector contains various bioactive compounds and plays a role in the transmission of pathogens to the host. The host develops anti-salivary antibodies against vector saliva exposure. Our previous research has identified two immunogenic proteins with molecular weights of 31 and 56 kDa from the Aedes aegypti salivary gland protein extract. However, the role of the 31 and 56 kDa immunogenic proteins from saliva Ae. aegypti is not fully known, so it is necessary to purify two immunogenic protein fractions to better specify the target of developing a dengue vaccine. This study aimed to purify the 31 and 56 kDa immunogenic protein fractions by electroelution and dialysis methods. The purification of the two protein fractions has been successful which were confirmed by the SDS-PAGE by the existence of single-band parallel to the positive control. These results were further supported by the dot blot analysis which showed a positive reaction in the form of dark spots in the two protein fractions which were reacted with dengue patients' serum, endemic healthy people, and neonates. These results indicated that the purified 31 and 56 kDa immunogenic protein fraction can be identified by specific antibodies.Keywords: dialysis, electroelution, immunogenic, purification, saliva  ABSTRAKKelenjar saliva vektor arthropoda mengandung berbagai senyawa bioaktif dan berperan dalam transmisi patogen ke tubuh inang. Tubuh inang mengembangkan antibodi anti-saliva terhadap paparan saliva vektor. Penelitian kami sebelumnya telah mengidentifikasi dua protein imunogenik dengan berat molekul 31 dan 56 kDa dari ekstrak protein kelenjar saliva Aedes aegypti. Namun demikian, peranan protein imunogenik 31 dan 56 kDa dari saliva Ae. aegypti belum diketahui sepenuhnya sehingga perlu dilakukan purifikasi dua fraksi protein imunogenik untuk lebih menspesifikkan target pengembangan vaksin dengue. Tujuan penelitian ini untuk melakukan purifikasi fraksi protein imunogenik 31 dan 56 kDa melalui metode elektroelusi dan dialisis. Keberhasilan purifikasi dua fraksi protein 31 dan 56 kDa terbukti dari hasil konfirmasi SDS-PAGE dengan terbentuknya pita tunggal sejajar dengan kontrol positif. Hasil tersebut diperkuat dengan analisis dot blot yang menunjukkan reaksi positif dengan munculnya noktah gelap pada dua fraksi protein tersebut ketika direaksikan dengan serum pasien DBD, penduduk sehat endemik dan neonatus. Hasil ini mengindikasikan bahwa fraksi protein imunogenik 31 dan 56 kDa hasil purifikasi dapat dikenali oleh antibodi spesifik.
OPTIMASI MEDIUM PERBANYAKAN IN VITRO TUNAS TALAS KALIURANG (Colocasia esculenta L.) DIPLOID DAN TETRAPLOID Khalisa Aini Sinaga; Dyah Retno Wulandari; Diah Ratnadewi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (602.22 KB) | DOI: 10.29122/jbbi.v7i2.3944

Abstract

Optimation of In Vitro Shoot Proliferation Medium for Diploid and Tetraploid Kaliurang Taro (Colocasia esculenta L.) Taro cv. Kaliurang has a good taste and is tolerant to certain pests and diseases but its development is hampered by insufficient number of good quality plant materials. Quality improvement has been carried out through polyploidization. Shoot multiplication is an important step in micropropagation, which often needs specific formulation of culture medium. This study aimed to obtain an optimum formulation of in vitro shoot-inducing medium of taro cv. Kaliurang. Explants from one diploid and three tretraploid clones were subjected to six treatments of medium formulations with various concentrations of thiamine and adenine in BAP-containing MS media. Shoots were then rooted, followed by plantlet acclimatization. Ploidy level was measured using flow cytrometry. The rooting medium was ½ MS without growth hormones, whereas acclimatization medium was a mixture of sterile soil, husk, and cocopeat. The results showed that MS + 2 mg L-1 BAP + 4 mg L-1 thiamine + 2 mg L-1 adenine was the optimum medium with an average 3.45 shoots per explant. Plantlet acclimatization was successful with 99.1% survival. Flow cytometry measurement confirmed tetraploidy level of the regenerants from 3 tetraploid clones. Keywords: adenine, acclimatization, benzyl amino purine, shoot proliferation, thiamine   ABSTRAK Talas Kaliurang memiliki rasa yang enak dan toleran terhadap hama dan penyakit tertentu, namun pengembangannya terkendala oleh ketercukupan benih bermutu. Upaya perbaikan mutu tanaman telah dilakukan sebelumnya melalui poliploidisasi. Perbanyakan tunas merupakan langkah penting, yang membutuhkan formula spesifik untuk media kultur. Penelitian ini bertujuan untuk mengoptimasi medium perbanyakan tunas in vitro dari satu klon diploid dan tiga klon tetraploid, yang dikulturkan pada enam konsentrasi tiamin dan adenin dalam media MS yang mengandung BAP. Tunas kemudian diinduksi akar, lalu diaklimatisasi, dan pengukuran tingkat ploidi menggunakan flow cytometry. Media pengakaran adalah ½ MS tanpa ZPT, media aklimatisasi adalah campuran tanah steril, sekam dan kokopit. Hasil penelitian ini menunjukkan bahwa medium MS + 2 mg L-1 BAP + 4 mg L-1 tiamin + 2 mg L-1 adenin merupakan medium optimum dengan rata-rata 3,45 tunas per-eksplan. Hasil aklimatisasi menunjukkan bahwa 99,1% tanaman dapat bertahan hidup. Analisis ploidi dengan flow cytometry menunjukkan bahwa tanaman hasil regenerasi tunas talas Kaliurang tetraploid memiliki tingkat ploidi yang stabil.
ANALISIS HASIL FRAKSINASI PROTEASE DAN LIPASE YANG BERASAL DARI SALURAN PENCERNAAN UDANG VANAME (Litopenaeus vannamei) Hanifah Rahmi; . Hariyanti; Rina Putri Ariyanti; Devi Wulandari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.212 KB) | DOI: 10.29122/jbbi.v7i2.3994

Abstract

Analysis of Protease and Lipase Fractionation Originated from the Digestive Tract of Vannamei Shrimp (Litopenaeus vannamei) Vannamei shrimp is a fishery commodity with a high consumption value, so it has an impact of high shrimp waste in the form of head and skin. The digestive tract connected to the head of the vaname shrimp (Litopenaeus vannamei) contains digestive enzymes, including proteases and lipases. This study aims to obtain the protein fraction that has the highest protease and lipase activity. The separation method used was centrifugation followed by precipitation using ammonium sulfate salt and dialysis. The dialysate was purified by gel filtration chromatography at a volume retention of 10 drops per tube. The proteolytic and lipolytic enzyme activity of the fraction was measured using a spectrophotometer. The results showed that fraction 102 had the highest protease activity value of 96.3924 U / mL, while fraction 100 had the highest lipase activity of 531.07 U / mL. This study showed that in the digestive tract of vaname shrimp, protease and lipase activity increased with the level of purity.  Keywords: digestive enzymes, gel filtration chromatography, lipase, protease, vannamei shrimp ABSTRAK Udang vaname merupakan komoditi perikanan dengan nilai konsumsi yang tinggi, sehingga berdampak pula dengan tingginya limbah udang yang berupa kepala dan kulit. Saluran pencernaan yang terhubung dengan kepala udang vaname (Litopenaeus vannamei) mengandung enzim pencernaan, diantaranya protease dan lipase. Penelitian ini bertujuan untuk mendapatkan fraksi protein yang memiliki aktivitas protease dan lipase tertinggi. Metode pemisahan yang dilakukan adalah sentrifugasi dilanjutkan dengan pengendapan menggunakan garam ammonium sulfat dan dialisis. Dialisat dimurnikan dengan kromatografi filtrasi gel pada retensi volume sebanyak 10 tetes tiap tabung. Aktivitas enzim proteolitik dan lipolitik fraksi diukur menggunakan spektrofotometer. Hasil menunjukkan bahwa fraksi 102 memiliki nilai aktivitas protease tertinggi sebesar 96,3924 U mL–1, sedangkan fraksi 100 memiliki aktivitas lipase tertinggi sebesar 531,07 U mL–1. Penelitian ini menunjukkan bahwa pada saluran pencernaan udang vaname terdapat aktivitas protease dan lipase yang meningkat seiring dengan tingkat kemurniannya.

Page 30 of 55 | Total Record : 542

 28   29   30   31   32 

Filter by Year

2014 2023


Reset
Filter By Issues
All Issue Vol. 10 No. 1 (2023): Juni 2023 Vol. 9 No. 2 (2022): December 2022 Vol. 9 No. 1 (2022): June 2022 Vol. 8 No. 2 (2021): December 2021 Vol. 8 No. 1 (2021): June 2021 Vol. 7 No. 2 (2020): December 2020 Vol. 7 No. 1 (2020): June 2020 Vol. 6 No. 2 (2019): December 2019 Vol. 6 No. 1 (2019): June 2019 Vol 6, No 1 (2019): June 2019 Vol. 5 No. 2 (2018): December 2018 Vol 5, No 2 (2018): December 2018 Vol 5, No 1 (2018): June 2018 Vol. 5 No. 1 (2018): June 2018 Vol. 4 No. 2 (2017): December 2017 Vol 4, No 2 (2017): December 2017 Vol. 4 No. 1 (2017): June 2017 Vol 4, No 1 (2017): June 2017 Vol. 3 No. 1 (2016): June 20160 Vol 3, No 1 (2016): June 20160 Vol. 3 No. 2 (2016): December 2016 Vol 3, No 2 (2016): December 2016 Vol. 3 No. 1 (2016): June 2016 Vol 3, No 1 (2016): June 2016 Vol. 2 No. 2 (2015): December 20150 Vol 2, No 2 (2015): December 20150 Vol. 2 No. 1 (2015): June 20150 Vol 2, No 1 (2015): June 20150 Vol 2, No 2 (2015): December 2015 Vol. 2 No. 2 (2015): December 2015 Vol 2, No 1 (2015): June 2015 Vol. 2 No. 1 (2015): June 2015 Vol. 1 No. 1 (2014): December 20140 Vol 1, No 1 (2014): December 20140 Vol 1, No 1 (2014): December 2014 Vol. 1 No. 1 (2014): December 2014 More Issue