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Jurnal Bioteknologi & Biosains Indonesia (JBBI)

JBBI
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Published by Badan Pengkajian dan Penerapan Teknologi

ISSN : 24422606 EISSN : 2548611X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.

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Articles 542 Documents

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BAKTERI ENDOFIT YANG DIISOLASI DARI AKAR Eurycoma longifolia DAN POTENSINYA SEBAGAI PENGENDALI JAMUR PATOGEN TANAMAN Tri Ratna Sulistiyani; Siti Meliah; . Damayanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Eurycoma longifoliaÂ (pasak bumi) is known as a medicinal plant that containsÂ biologically active compounds. Studies on endophytic bacteria associated with pasak bumi andÂ theirÂ biocontrol activitiesÂ have not been widely reported.Â The objective of this study is to isolate potential endophytic bacteria associated with E. longifolia possessing biocontrol activity against plant pathogenic fungi, Sclerotium rolfsii, Fusarium solani, F. oxysporum,Â andÂ Colletotrichum gloeosporioides.Â Endophytic bacteria were isolated from the roots of E. longifolia using the plant piece methodÂ and identifiedÂ based on 16S rRNA genes analysis.Â Antagonist test of bacterial isolates was conductedÂ byÂ dual confrontation method.Â TheÂ mechanisms of fungal growth inhibition were evaluated based on their ability to produce hydrolytic enzymes, antibiotic,Â and volatile organic compounds. Two isolates were obtained and identified as Stenotrophomonas maltophilia (Apb1) and Serratia marcescens (Apb2). Apb2 was able toÂ inhibitÂ the growth ofÂ four tested fungi andÂ showed protease, chitinaseÂ as well asÂ cellulase activities. The crude extract and volatile organic compound produced by Apb2Â wereÂ active against F.Â solaniÂ growth.Keywords: biocontrol, endophytic, Eurycoma longifolia, fungi, inhibition mechanismÂ ABSTRAKEurycoma longifoliaÂ (pasak bumi) dikenal sebagai tanaman obat yang mengandungÂ beberapa senyawa aktif secara biologis. PenelitianÂ mengenaiÂ bakteri endofit yang berasosiasi dengan tanaman pasak bumi berikut aktivitasÂ biokontrolnya belumÂ banyakÂ dilaporkan.Â Penelitian ini bertujuan mengisolasi bakteri endofit potensial dari tanamanÂ E. longifoliaÂ yang memiliki aktivitas biokontrol terhadap empat strain uji jamur patogen tanaman, yaitu Sclerotium rolfsii, Fusarium solani, F. oxysporum, dan Colletotrichum gloeosporioides. Bakteri endofit diisolasi dari akarÂ E. longifolaÂ menggunakan metodeÂ plant piece dan diidentifikasi berdasarkan analisis gen 16S rRNA.Â Uji antagonis isolat bakteriÂ dilakukan dengan metode konfrontasi ganda.Â Mekanisme penghambatan jamur patogen tanaman dievaluasi berdasarkan kemampuannya dalam memproduksi enzim hidrolisis, senyawa antibiotik, dan senyawa organik volatil.Â Dua isolat bakteri endofit berhasil diperoleh dan teridentifikasi sebagaiÂ Stenotrophomonas maltophilia Â (Apb1) dan Serratia marcescens (Apb2). Isolat Apb2 mampu menghambat pertumbuhan keempat jamur yang diujiÂ dan menunjukkan aktivitas protease, kitinase dan selulase. Ekstrak kasar dan senyawa organik volatil yang dihasilkan oleh isolat Apb2 aktif menghambatÂ pertumbuhanÂ F. solani.

DETEKSI VARIASI SOMAKLONAL PLANLET Jatropha curcas Linn. HASIL REGENERASI EMBRIO SOMATIK DENGAN MARKA MOLEKULAR ISSR . Rudiyanto; Darda Efendi; Erwin Al-Hafiizh; Tri Muji Ermayanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Physic nut (Jatropha curcas Linn.) has the potential as a source of sustainable biofuels. Somatic embryo proliferation of J. curcas may cause somaclonal variations. This research aimed to investigate somaclonal variations of J. curcas somatic embryo derived-plantlet using ISSR markers. Somatic embryos of J. curcas at the globular phase were cultured on liquid MS medium supplemented with 0, 0.5, 1.0, 1.5, and 2.0 mg L-1 of 2,4-D. Parameter observed were embryos weight, embryos volume, colour, and size of embryos. After proliferation, the embryos were cultured on a germination medium until the cotyledonary phase. The results showed that proliferation of J. curcas somatic embryos was optimal, with the highest weight and volume, at MS medium added with 1 mg L-1 2,4-D.

EFEK ANTIBAKTERI EKSTRAK DAUN CENGKEH (Syzygium aromaticum) TERHADAP Escherichia coli DAN Staphylococcus aureus Ardia Ramadhani; Susy Saadah; Sogandi Sogandi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Antibacterial Effect of Clove Leaf Extract (Syzygium aromaticum) against Escherichia coli and Staphylococcus aureus Escherichia coli and Staphylococcus aureus are microorganisms that cause infection. Overcoming infection using antibiotics is known to generate bacteria that are resistant to some antibiotics, hence the need of other antibacterial resources. One of the natural sources that can be utilized is clove leaf (Syzygium aromaticum). This study aims to determine the types of compounds contained in clove leaves and their inhibitory activity against E. coli and S. aureus. The study began with extraction using maceration techniques, then the separation of the compounds through ethanol, n-hexane, and ethyl acetate fractionation. Next step was the identification of secondary metabolites of clove leaf compounds using Gas Chromatography-Mass Spectrometry (GC-MS). The results showed the ethyl acetate fraction was the most active in inhibiting the growth of E. coli and S. aureus with a minimum inhibitory value (MIC) of 10%. The most dominant compound in the ethyl acetate fraction was found to be caffeine with a content of 23.36%. Keywords: antibacterial, clove leaves, GC-MS, MIC, Syzygium aromaticum ABSTRAK Escherichia coli dan Staphylococcus aureus adalah mikroorganisme penyebab infeksi. Penanggulangan infeksi menggunakan antibiotik telah memunculkan bakteri yang resisten terhadap beberapa antibiotik sehingga perlu mencari sumber antibakteri lain. Salah satu bahan alam yang dapat dimanfaatkan adalah daun cengkeh (Syzygium aromaticum). Penelitian ini bertujuan mengetahui jenis senyawa yang terkandung dalam daun cengkeh dan aktivitas penghambatannya terhadap bakteri E. coli dan S. aureus. Penelitian diawali dengan ekstraksi menggunakan teknik maserasi, kemudian dilakukan pemisahan senyawa berdasarkan tingkat kepolaran melalui fraksinasi etanol, n-heksan, dan etil asetat. Hasil fraksinasi diujikan ke bakteri uji. Identifikasi senyawa metabolit sekunder daun cengkeh menggunakan Gas Chromatography Mass Spectrometry (GC-MS). Hasil penelitian menunjukkan bahwa fraksi etil asetat paling aktif menghambat pertumbuhan bakteri E. coli dan S. aureus dengan nilai kadar hambat minimum (KHM) 10%. Senyawa paling dominan pada fraksi etil asetat adalah kafein dengan kadar 23,36%.

POTENSI SENYAWA BIOAKTIF TANAMAN KELOR PENGHAMBAT INTERAKSI ANGIOTENSIN-CONVERTING ENZYME 2 PADA SINDROMA SARS-COV-2 Maharani Pertiwi Koentjoro; Adyan Donastin; Endry Nugroho Prasetyo
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

The Potential of Moringa oleifera Bioactive Compounds for Inhibiting Angiotensin-Converting Enzyme 2 Interaction in SARS-Cov-2 Syndrome Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) disease (COVID-19) is a threat to human health. This infection is determined by the interaction of the spike S1 domain protein with angiotensin-converting enzyme 2 (ACE2) in the epithelial cells of the respiratory tract, especially the lungs. ACE2 inhibition is an important target in controlling COVID-19. Flavonoids of medicinal plants, are known to interfere with ACE (ACE2 homologous). Therefore, this study aims to explore the ability of apiin, epicatechin, and hesperetin from Moringa oleifera in interacting with the ACE2 using MOE 2008.10. The ligand molecules were prepared from PubChem database. The ACE2 protein was retrieved from Protein Data Bank (ID 1R4L) and analyzed for the active sites. Analysis of docking scores and hydrogen bonds of ACE2-ligand complex and active site showed that the affinity of flavonoids can be ranked as hesperetin > epicatechin > apiin > C19H23Cl2N3O4. The results provided computational information that apiin, epicatechin, and hesperetin have the potential to prevent COVID-19 infection. The prediction of activity spectra for substances (PASS) score showed the ligand displays antiviral activity. Infeksi severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pada pandemi coronavirus disease 2019 (COVID-19) menjadi ancaman dunia kesehatan saat ini. Infeksi SARS-CoV-2 ditentukan oleh interaksi protein spike envelope S1 domain dengan reseptor angiotensin-converting enzyme 2 (ACE2) yang diekspresikan pada sel epitel saluran pernafasan terutama paru-paru. Mekanisme penghambatan ACE2 menjadi target penting dalam pengendalian COVID-19. Senyawa bioaktif tanaman obat, seperti flavonoid diketahui mampu mengganggu fungsi banyak makromolekul termasuk ACE (homolog dengan ACE2). Penelitian ini bertujuan mengeksplorasi kemampuan senyawa apiin, epicatechin, dan hesperetin dari Moringa oleifera dalam berinteraksi dengan sisi aktif ACE2 menggunakan metode penambatan molekul. Studi dilakukan dengan preparasi struktur molekul ligan dari PubChem database dan diolah dengan MOE 2008.10. Selanjutnya, data protein ACE2 (Protein Data Bank ID 1R4L) dianalisis sisi aktifnya untuk mengetahui lokasi penambatan ligan senyawa. Analisis skor docking dan ikatan hydrogen komplek ligan dan sisi aktif ACE2 menunjukkan bahwa afinitas flavonoid dapat diperingkatkan sebagai afinitas hesperetin > epicatechin > apiin > C19H23Cl2N3O4. Ketiga ligan senyawa yang terkandung dalam M. oleifera secara in silico mampu mengikat sisi aktif ACE2, sehingga berpotensi mencegah infeksi COVID-19. Skor PASS (prediction of activity spectra for substances) menunjukkan aktivitas biologis ligan yang menyerupai antiviral.

Back Cover JBBI Vol 7, No 1, December 2020 . .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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AKTIVITAS ANTIBAKTERI EKSTRAK BIJI JERUK SIAM (Citrus reticulata) PADA BAKTERI Escherichia coli Mohammad Arfi Setiawan; Mita Dewi Retnoningrum; Febriyandhi Yahya; Resa Ragil Andika; Dyan Hatining Ayu Sudarni
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Antibacterial Activity of Citrus seed (Citrus reticulata) Extract on Escherichia coli Indonesian agriculture provides a resource of medicinal plants whose potential needs to be explored in order to benefit society. One of them is the use of Siam orange seeds (Citrus reticulata) which has the potential for the production of antibacterial compounds. This study aims to test the antibacterial activity of the ethanol and n-hexane extract of orange seeds. The extract was obtained through maceration techniques using ethanol and n-hexane as solvents. The antibacterial activity test of orange seeds against Escherichia coli used the paper disc diffusion method with nutrient agar (NA) media. The concentration of orange seed extract for the determination of MIC (Minimum Inhibitory Concentration) was 0.5, 2, 8, 10, 20 mg mL-1. The results showed that the ethanol and n-hexane extract of orange seeds had antibacterial activity against E. coli. However, the ethanol extract had a higher antibacterial effect than the n-hexane orange seed extract. From the results of this study, it is hoped that the waste of orange seeds will provide beneficial contribution for pharmaceutical development. Pertanian Indonesia memiliki sumber tanaman obat yang perlu digali potensinya agar bermanfaat bagi masyarakat. Salah satunya pemanfaatan biji jeruk siam (Citrus reticulata) yang berpotensi menghasilkan senyawa antibakteri. Penelitian ini bertujuan untuk menguji aktivitas antibakteri ekstrak etanol dan n-heksana biji jeruk. Ekstrak diperoleh melalui teknik maserasi menggunakan pelarut etanol dan n-heksana. Uji aktivitas antibakteri biji jeruk terhadap Escherichia coli menggunakan metode difusi paper disc dengan media nutrient agar (NA). Konsentrasi ekstrak biji jeruk untuk penentuan MIC (Minimum Inhibitory Concentration) adalah 0,5, 2, 8, 10, 20 mg mL-1. Hasil penelitian menunjukkan bahwa ekstrak etanol dan n-heksana biji jeruk memiliki aktivitas antibakteri terhadap E. coli. Namun, ekstrak etanol memiliki efek antibakteri yang lebih tinggi dibandingkan ekstrak biji jeruk n-heksana. Dari hasil penelitian ini, limbah biji jeruk diharapkan dapat memberikan kontribusi bermanfaat bagi pengembangan farmasi.

PENGARUH METODE PROSES SINTESIS ANALOG KURKUMIN ASIMETRIS TERHADAP EFEK HEPATOPROTEKTIF MENCIT (Mus musculus L.) Imanuel Berly Delvis Kapelle; Wasmen Manalu
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Effect of Synthesis Process Method of Asymmetric Curcumin Analog on the Hepatoprotective Effect of Mice (Mus musculus L.) Asymmetric curcumin analogs (ACA) can be synthesized from cullilawan oil. ACA products can be synthesized using conventional methods and microwaves. Synthesis methods can affect physical properties and pharmacological effects. The purpose of this study was to determine the effect of the process method on the hepatoprotective ability of ACA and to determine the effective dose. ACA products were tested in vivo in mice (Mus musculus L.) with CCl4-induced liver damage. The parameters observed were biochemical liver enzymes (AST and ALT) and histological analysis. The results showed that animals treated with ACA-k products had better weight gain, lower AST and ALT levels, and fewer histological signs of liver damage at higher ACA doses than those observed in animals that were untreated or treated with ACA-m products. Thus, asymmetric curcumin analog products that were processed by conventional method were more hepatoprotective. Keywords: ACA, culilawan oil, hepatoprotection, in vivo, product diversification ABSTRAK Analog kurkumin asimetris (AKAS) dapat disintesis dari minyak kulit lawang. Produk AKAS dapat disintesis menggunakan metode konvensional dan microwave. Metode sintesis dapat mempengaruhi sifat fisik dan efek farmakologis. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh metode proses pada kemampuan hepatoprotektif AKAS dan untuk menentukan dosis efektif. Produk AKAS diuji in vivo pada mencit (Mus musculus L.) dengan kerusakan hati yang diinduksi CCl4. Parameter yang diamati adalah biokimia enzim hati (AST dan ALT) dan analisis histologis. Hasil penelitian menunjukkan bahwa hewan yang diobati dengan produk AKAS-k memiliki kenaikan berat badan yang lebih baik, tingkat AST dan ALT yang lebih rendah, dan lebih sedikit tanda histologis kerusakan hati pada dosis AKAS yang lebih tinggi daripada yang terlihat pada hewan yang tidak diperlakukan atau diobati dengan produk AKAS-m. Dengan demikian, produk analog kurkumin asimetris yang diproses dengan metode konvensional lebih hepatoprotektif.

TELAAH KOMPREHENSIF DIABETES MELITUS: KLASIFIKASI, GEJALA, DIAGNOSIS, PENCEGAHAN, DAN PENGOBATAN: A Comprehensive Review of Diabetes Mellitus: Classification, Symptoms, Diagnosis, Prevention, and Treatment Dudi Hardianto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia. In general, diabetes is classified into type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM), gestational, and other specific diabetes. The causes of diabetes are genetic disorders and environmental. Common symptoms of diabetes include: polydipsia, polyphagia, glycosuria, polyuria, dehydration, fatigue, weight loss, reduced vision, cramps, constipation, and candida infection. Test for diagnosis of diabetes include: fasting plasma glucose test, plasma glucose test after 2 hours of 75 g oral glucose administration, the glycated hemoglobin test (HbA1C), and random blood glucose test. Prevention of T1DM is still difficult because of the limited knowledge of metabolic, genetic, and immunological processes in the development of T1DM. T2DM is prevented by lifestyle and medical intervention. Insulin is the only drug for T1DM, whereas T2DM is treated with metformin as drug’s primary choice for reducing blood glucose levels. Diabetes melitus merupakan penyakit kelainan metabolisme yang ditandai dengan hiperglikemia. Secara umum, diabetes diklasifikasikan menjadi: diabetes melitus tipe 1 (DMT1), diabetes melitus tipe 2 (DMT2), gestasional, dan diabetes spesifik lain. Penyebab diabetes adalah kelainan genetik dan lingkungan. Gejala umum diabetes antara lain: polidipsia, polifagia, glikosuria, poliuria, dehidrasi, kelelahan, penurunan berat badan, daya penglihatan berkurang, kram, konstipasi, dan infeksi candida. Pemeriksaaan untuk diagnosis diabetes meliputi: pemeriksaan glukosa plasma saat puasa, pemeriksaan glukosa plasma setelah 2 jam pemberian glukosa oral 75 g, pemeriksaan hemoglobin terglikasi (HbA1C), dan pemeriksaan glukosa darah acak. Pencegahan DMT1 masih sulit karena terbatasnya pengetahuan proses metabolisme, genetik, dan imunologi pada perkembangan DMT1. DMT2 dicegah dengan intervensi gaya hidup dan intervensi medis. Insulin merupakan satu-satunya obat untuk DMT1, sedangkan DMT2 diobati dengan metformin sebagai pilihan utama dan non obat untuk menurunkan kadar glukosa dalam darah.

MYCELIAL AMYLASE AND CELLULASE CHARACTERIZATION AS WELL AS BASIDIOMA PHYSICOCHEMICAL ANALYSIS OF LINGZHI MUSHROOM Yati Sudaryati Soeka; Muhammad Ilyas
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Karakterisasi Amilase dan Selulase Miselial Serta Analisis Fisikokimia Basidioma Jamur Lingzhi (Ganoderma lucidum) Lingzi mushroom (Ganoderma lucidum) synthesizes enzymes which have anti-hyperglycemic and anti-diabetic activities. This preliminary study aims to characterize the amylase and cellulase activities of mycelial culture, and to analyze the physicochemical compounds in the basidioma of G. lucidum InaCC F11 and G. lucidum InaCC F106. The enzymes were characterized spectrophotometrically using DNS method, and the basidioma was subjected to proximate and high performance liquid chromatography (HPLC) analysis, as well as microstructural observation using scanning electron microscope. Results showed both strains demonstrated amylase activity, but not cellulase activity. The optimum activity of amylase in G. lucidum InaCC F11 mycelial cultures was achieved on the 3rd incubation day, at pH 5.5, 35 ºC temperature, and 1.5% substrate concentration, whereas that of G. lucidum InaCC F106 on the 7th incubation day, at pH 5, 40 ºC temperature, and 1.75% substrate concentration. Dried basidioma of G. lucidum InaCC F11 contained 93.72% carbohydrates, 3.06% protein, 0.85% fat, 0.768% crude fiber, 0.54% ash, and 1.83% moisture. In addition, HPLC detected the presence of phenols (0.036%), steroids (0.014 mg 100 mL-1), and active triterpenoid compounds. Jamur lingzi (Ganoderma lucidum) mensintesis enzim yang memiliki aktivitas anti-hiperglikemik dan anti-diabetes. Studi pendahuluan ini bertujuan mengkarakterisasi aktivitas amilase dan selulase kultur miselium, serta menganalisis senyawa fisikokimia pada basidioma G. lucidum InaCC F11 dan G. lucidum InaCC F106. Enzim dikarakterisasi secara spektrofotometri menggunakan metode DNS. Basidioma dianalisis secara proksimat, menggunakan kromatografi cair kinerja tinggi (KCKT), serta diamati mikrostrukturnya menggunakan mikroskop elektron. Hasil menunjukkan kedua strain tersebut memiliki aktivitas amilase, dan tidak ada aktivitas selulase. Aktivitas amilase optimum pada kultur miselium G. lucidum InaCC F11 dicapai pada inkubasi hari ke-3, pH 5,5, suhu 35 ºC, dan konsentrasi substrat 1,5%, sedangkan pada kultur miselium G. lucidum InaCC F106 dicapai pada inkubasi hari ke-7, pH 5, suhu 40 ºC, dan konsentrasi substrat 1,75%. Basidioma kering G. lucidum InaCC F11 mengandung karbohidrat 93,72%, protein 3,06%, lemak 0,85%, serat kasar 0,768%, abu 0,54%, dan kadar air 1,83%. Selain itu, KCKT mendeteksi adanya fenol (0,036%), steroid (0,014 mg 100 mL-1), dan senyawa triterpenoid aktif.

CONFIRMATION OF ENDOPHYTIC MICROBES CAUSING CONTAMINATION IN WATER SPINACH (Ipomoea aquatica Forssk.) TISSUE CULTURE Resa Sri Rahayu; Indriati Ramadhani; Masrukhin Masrukhin; Indira Riastiwi; Apriliana Dyah Prawestri; Yeni Yuliani
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Konfirmasi Mikroba Endofit Penyebab Kontaminasi pada Kultur Jaringan Kangkung (Ipomoea aquatica Forssk.) Tanaman kangkung secara alami bersimbiosis dengan mikroba endofit, yang berpotensi menjadi kontaminan pada kultur jaringan kangkung karena berada di dalam jaringan dan sulit dijangkau saat proses sterilisasi eksplan. Tujuan penelitian ini adalah mengonfirmasi mikroba endofit penyebab kontaminasi pada kultur jaringan kangkung ‘Tetraploid’ sehingga dapat menjadi informasi awal untuk metode sterilisasi yang efektif. Sebanyak 14 sampel kontaminan pada media tanam kultur jaringan kangkung diisolasi dan diidentifikasi secara molekuler berdasarkan gen 16S rDNA untuk bakteri, daerah D1/D2 dari gen LSU rRNA untuk khamir, dan daerah ITS dari gen rDNA untuk jamur. Keragaman jenis mikroba yang teridentifikasi dibandingkan dengan keragaman jenis mikroba endofit dari jaringan tanaman kangkung yang ditanam pada media kultur jaringan tidak terkontaminasi mikroba, media kultur jaringan terkontaminasi mikroba, media tanam campuran tanah steril dan tidak steril, serta kangkung yang didapatkan dari pasar. Hasil penelitian ini mengonfirmasi bahwa khamir endofit dari kelompok Ustilaginaceae (basidiomycetous yeast) yang berasal dari jaringan tanaman kangkung sama dengan jenis kontaminan yang mengontaminasi media kultur jaringan kangkung ‘Tetraploid’. Khamir dari kelompok Ustilaginaceae (basidiomycetous yeast) tersebut juga merupakan mikroba paling dominan yang mengontaminasi media tanam kultur jaringan kangkung ‘Tetraploid’. Keywords: Ipomoea aquatica, kultur jaringan, mikroba endofit, morfologi, pohon filogeni Water spinach in nature lives in symbiosis with endophytic microbes, which have the potential to become contaminants in water spinach tissue culture because they are difficult to eliminate during the explant sterilization process. This study aimed to confirm endophytic microbes that cause contamination in the tissue culture of 'Tetraploid' water spinach so that it can provide initial information for an effective sterilization method. Fourteen contaminant samples in water spinach tissue culture media were isolated and identified molecularly based on the 16S rDNA gene for bacteria, the D1/D2 region of the LSU rRNA gene sequences for yeast, and ITS region of the rDNA gene for mold. The diversity of microbial species identified was compared with the diversity of endophytic microbial types from water spinach plant tissue grown on sterile tissue culture media, microbially contaminated tissue culture media, sterile and non-sterile soil mixed planting media, and water spinach obtained from the market. The results confirmed that endophytic yeast from Ustilaginaceae group (basidiomycetous yeast) derived from water spinach plant tissue was the same type of microbe that contaminated the 'Tetraploid' water spinach tissue culture media. The results gave new information that yeast from the Ustilaginaceae (basidiomycetous yeast) group was the most dominant microbe contaminating water spinach ‘Tetraploid’ tissue culture media. This group is endophytic yeast that lives within Ipomoea aquatica tissues.

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