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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
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Back Cover JBBI Vol 7, No 2, December 2020 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Front Cover JBBI Vol 7, No 2, December 2020 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Appendix JBBI Vol 7, No 2, December 2020: Keyword Index and Author Index .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Preface JBBI Vol 7, No 2, December 2020: Foreword and Acknowledgement .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 2 (2020): December 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

PROLIFERATION OF OIL PALM (Elaeis guineensis Jacq.) EMBRYOGENIC CALLUS WITH REPEATED SUBCULTURES IN LIQUID MEDIUM Karyanti, Karyanti; Tajuddin, Teuku; Khairiyah, Hayat; Purwoko, Devit; Sukarnih, Tati; Rahmadara, Gemilang; Hanifah, Nurul Fitri; Rudiyana, Yayan; Kitagawa, Sayuri; Mira, Farida Rosana; Saga, Hirohisa
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (977.075 KB) | DOI: 10.29122/jbbi.v8i1.4715

Abstract

The availability of high-quality seeds is now a necessity. This is due to a government program to replace oil palm trees in smallholder plantations with high quality seeds. An efficient protocol to produce a large number of embryos is needed. To increase the number of embryogenic callus production, the callus proliferation experiment was carried out through suspension culture. This study aimed to examine the proliferation ability of embryogenic callus from three different oil palm clones, in several repeated subcultures. Liquid MS media added with 1 ppm 2.4-D and 0.1 ppm NAA were used. Embryogenic callus was weighed by 0.1 - 0.2 g, transferred into the liquid media, shaking at 60-80 rpm and 27 ºC for 8 weeks without light. Continues subcultures were repeated up to 7 times. The results showed that the growth rate of embryogenic callus increased in the third and fourth subcultures and then decreased in subsequent subcultures. It also revealed that the entire embryogenic callus from the first subculture up to seventh subculture still has the ability to regenerate into new plants. These results indicate that oil palm embryogenic callus can be proliferated by suspension culture with a limit up to the fourth subculture. Ketersediaan benih kelapa sawit berkualitas saat ini merupakan kebutuhan karena adanya program pemerintah untuk menggantikan tanaman sawit di kebun-kebun petani. Salah satu cara vegetatif yang dapat dilakukan adalah meningkatkan jumlah kalus embriogenik yang dihasilkan melalui pengembangan kultur suspensi. Penelitian ini bertujuan mengkaji kemampuan proliferasi kalus embriogenik dari tiga klon kelapa sawit, pada beberapa kali subkultur yang berulang. Media cair MS dengan penambahan 1 ppm 2,4-D dan 0,1 ppm NAA digunakan untuk memperbanyak 0,1–0,2 g kalus embriogenik, dikocok pada 60-80 rpm dan suhu 27 ºC tanpa cahaya selama 8 minggu. Subkultur berulang dilakukan hingga 7 kali. Hasil percobaan menunjukkan bahwa kemampuan proliferasi kalus dipengaruhi oleh genotip tanaman induk. Rata-rata kalus embriogenik dapat meningkat pada subkultur ke-3 dan ke-4 dan semakin menurun pada subkultur selanjutnya. Kalus embriogenik hasil proliferasi subkultur pertama hingga ke-7 dapat tumbuh menjadi calon tanaman baru. Hasil ini menunjukkan bahwa kalus embriogenik kelapa sawit dapat diperbanyak dengan kultur suspensi pada batas sampai subkultur ke-4.
PERUBAHAN KANDUNGAN ASAM FITAT DAN ASAM AMINO ESENSIAL BAHAN-BAHAN ORGANIK PAKAN YANG DIFERMENTASI RAGI TEMPE Muniroh, Arina; Suja'i, Imam; Wibowo, Aji; Saputra, Henry Kasman Hadi; Yunita, Etyn; Sriherwanto, Catur
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (996.834 KB) | DOI: 10.29122/jbbi.v8i1.4743

Abstract

Changes in the Contents of Phytic Acid and Essential Amino Acids of Animal Feed Organic Materials Fermented by Tempe Starter The contents of antinutrients and nutrients are important in the selection of feed ingredients, especially for monogastric animals. The aim of this study is to determine the change in the contents of antinutrient phytic acid and nutrient essential amino acid in selected organic materials. Nine organic ingredients of agro-industrial by-products, namely rice bran, coffee skin, cassava bagasse, corn, coconut dreg, soy bean meal, pollard, corn gluten feed (CGF), and copra meal were fermented in solid state using tempeh starter for 48 hours at 30°C. Dense mycelia overgrowing evenly on the top, bottom, and cross-sliced surfaces was observed on rice bran, maize and copra meal substrates. These 3 selected organic materials were then analysed for the content of phytic acid and essential amino acids. The results showed that the lowest decrease (75.80%) in phytic acid content occurred in maize, namely from 18.49 ± 0.41 mg g-1 (before fermentation) to 4.48 ± 0.19 mg g-1 (after fermentation). The highest increase (59%) of total essential amino acids occurred in copra meal, namely from 38,991.89 ± 447.12 mg kg-1 (before fermentation) to 61,816.56 ± 894.24 mg kg-1 (after fermentation). Kandungan antinutrisi dan nutrisi merupakan hal penting dalam pemilihan bahan pakan, terutama untuk hewan monogastrik. Penelitian ini bertujuan mengetahui perubahan kandungan antinutrisi asam fitat dan nutrisi asam amino esensial pada bahan organik tertentu yang terpilih. Sembilan bahan organik yang merupakan hasil samping agroindustri, yakni dedak padi, kulit kopi, onggok, jagung, ampas kelapa, bungkil kedelai (soy bean meal), dedak gandum (pollard), produk samping jagung corn gluten feed (CGF), dan bungkil kopra difermentasi padat menggunakan ragi tempe selama 48 jam pada suhu 30°C. Miselium yang tumbuh subur, padat, dan merata pada permukaan atas, bawah, dan irisan melintang teramati pada dedak padi, jagung, dan bungkil kopra. Tiga bahan organik terpilih ini kemudian dianalisa kandungan asam fitat dan asam amino esensialnya. Hasil menunjukkan bahwa penurunan terbesar (75,80%) kandungan asam fitat terjadi pada jagung, yakni dari 18,49 ± 0,41 mg g-1 (sebelum fermentasi) menjadi 4,48 ± 0,19 mg g-1 (setelah fermentasi). Peningkatan tertinggi (59%) asam amino esensial total terjadi pada bungkil kopra, yakni dari 38.991,89 ± 447,12 mg kg-1 (sebelum fermentasi) menjadi 61.816,56 ± 894,24 mg kg-1 (setelah fermentasi).
Back Cover JBBI Vol 8, No 1, June 2021 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Front Cover JBBI Vol 8, No 1, June 2021 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Appendix JBBI Vol 8, No 1, June 2021: Keyword Index and Author Index .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Preface JBBI Vol 8, No 1, June 2021: Foreword and Acknowledgement .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 1 (2021): June 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Page 33 of 55 | Total Record : 542

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