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Medical Laboratory Technology Journal

JAK
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Published by Poltekkes Kemenkes Banjarmasin

ISSN : - EISSN : 24610879 DOI : https://doi.org/10.31964/mltj

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have achieved in the area of medical laboratory sciences. This Journal particularly focuses on the main problems in the development of the sciences of medical laboratory areas. It covers the parasitology, bacteriology, virology, hematology, clinical chemistry, toxicology, food and drink chemistry, and any sciences that cover sciences of medical laboratory area.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 11 Documents

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Search results for , issue "Vol. 8 No. 1 (2022): June" : 11 Documents clear

Influence of Diabetes on Morphometric Index of Ovarian Follicles in Streptozocin-Induced Rats Serpil Ünver Saraydin; Berna Özdenoglu Kutlu; Dursun Saraydin
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The study aimed to determine the effect of diabetes on follicle development by measuring the diameters of rats' ovarian follicles. The structure and function of many organs change by being affected by diabetes. The ovary is an essential organ of the reproductive system affected by diabetes. The size of the ovarian follicles and corpus luteum can also be affected by diabetes. For this reason, body mass, blood glucose level, and rat ovarian follicles, and corpus luteum diameters were measured in this study. Ten healthy female rats were kept as the control group. In the other rat group, experimental diabetes was induced with streptozotocin (STZ)(60 mg/kg). The rats in both groups were killed after 30 days and their ovaries removed. 5-6 µm sections were made using paraffin embedding techniques and stained with hematoxylin-eosin. On the fifth day of STZ administration to rats, the mass loss of rats was 10%, and the diabetogenic index was 330%. Compared with the control group, diameters of diabetic rats significantly decreased the diameter of the primordial, primary and Graafian follicles, and corpus luteum. The decrease in the diameter of secondary follicles of diabetic rats was not significant. The percentage shrinkage index was the highest in the corpus luteum with 37%. As a result, it can be said that diabetes influences the size of the ovarian follicles and especially the corpus luteum, thereby negatively affecting the ability to improve oocyte quality. Diabetes-related follicle diameter may shrink and cause infertility. It may be essential to measure the diameters of the follicles in vitro fertilization studies in patients with diabetes.

Condition Optimization for The Analysis of Risperidone and 9-0H-Risperidone by High-Performance Liquid Chromatography Luki Yogaswara Yusuf; Aliya Nur Hasanah; Melisa Intan Barliana
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Risperidone (RIS) is one of the most widely used atypical antipsychotics for treating schizophrenia in hospitals. RIS is metabolized by the liver and produces the primary active metabolite 9-OH-Risperidone (9-OHR). In the process of RIS metabolism, it is suspected that there are gene polymorphisms that cause variations in patient responses. Analysis of RIS and 9-OHR levels in the patient's blood can help to explain the various responses. High-Performance Liquid Chromatography (HPLC) is the most popular method to analyze RIS and 9-OHR, but many deficiencies were found in the chromatograms in the previous study. This research aims to obtain optimal conditions of the analysis prior to method validation. Condition optimization by optimizing the wavelength, composition of the mobile phase, pH, flow rate, and particle size of the stationary phase. The results showed that the wavelength was 279 nm, the mobile phase was 0.05 M KH2PO4 pH 3.7: acetonitrile (94:6, v/v) plus 0.3% triethylamine, and the flow rate was 1.2 ml/min in the stationary phase (LiChroCART® RP 18; 250x4 mm; 10 µm) being the optimal condition. This method is suggested to continue method validation for analyzing RIS and 9-OHR in the serum or plasma.

Detection of the Metabolic Relationship Between Primary Tumor in Breast Cancer According to Molecular Classification in Positron Emission Tomography; Retrospective Cohort Study Ahmet Alyanak; Ferit Aslan
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The main objective of the study was to investigate whether the maximum SUV emission tomography values of Positron differ between luminal molecular properties. The second objective was to examine the relationship between positron emission tomography SUV max and ki67 in primary tumors and axillary lymphadenopathy. In the study between January 2018 and December 2020, 158 patients with stage 1-2-3 breast cancer admitted to the outpatient clinic of general surgery and medical oncology Yüksek htisas University Medicalpark Ankara were retrospectively evaluated. The results of the study considering the relationship between molecular properties and metabolic activity of primary tumors, found a significant relationship between grade (p<0.005), estrogen receptor (p:0.019), and progesterone receptor (p:0.045). More important differences were observed in the luminal type, especially between such as basal and luminal A (p: 0.021). A significant correlation was found in the Pearson correlation test, which was performed between the primary tumor and the SUV values for maxillary axillary lymphadenopathy (p < 0.001, correlation coefficient: 0.331). For Ki67, there was a significant, albeit low, correlation between the SUV max primary tumors (p: 0.026, correlation coefficient 0.179). Although there is a statistically positive trend between Ki67 and axillary SUV max, there is no significant difference (p: 0.06 correlation coefficient: 0.157) In conclusion, we found a significant relationship between max. positron emission tomography SUV, estrogen receptor, progesterone receptor, grade, ki67, and molecular subtypes such as basal and luminal A of the tumor. We found a correlation between the primary tumor and the metabolic activity of axillary lymphadenopathy. It will be meaningful to plan treatment and follow-up according to these results.

Analysis of Metabolite Levels, Secondary Minerals and Aloe Vera Formulation from Kalimantan Indonesia Fahmi Said; Ida Rahmawati; Neny Setiawaty Ningsih
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Products from natural ingredients such as aloe vera must be high quality and meet standardization aspects. Determination of secondary metabolites and mineral levels is part of the standardization of natural product products. The dosage formula needs to be optimized to get the best formula based on evaluating the physical properties of the gel preparation. This study aimed to analyze the highest mineral and metabolite content between aloe vera from South Kalimantan and West Kalimantan, as well as the optimum formula for aloe vera gel based on its physical properties. The research method used is to perform sample preparation, extraction, analysis of minerals and secondary metabolites by spectroscopy. The data were analyzed descriptively, and the results showed that the content of iron, calcium, and zinc originating from West Kalimantan was 0.314 mg/g; 93.42 mg/g; 0.059 mg/g, while South Kalimantan 0.064 mg/g, 53.24 mg/g, 0.032 mg/g. The total phenolic, flavonoid, and anthraquinone levels from West Kalimantan were 0.512%, 1.31%, respectively, 2.28%, while those from South Kalimantan were 0.321%, 1.12%, 1.14%. The best formula for aloe vera gel is formula three, which has a darker color and meets the requirements of the physical properties of the gel. This study concludes that the highest mineral content in aloe vera comes from West Kalimantan, the highest secondary metabolite content also comes from West Kalimantan. And the best formula is the third formula with 20% natural dyes. Suggestions for further research are to examine the mineral content and secondary metabolites of aloe vera in various places in Indonesia so that the best aloe vera can be known.

Utility of Neutrophil Lymphocyte Ratio (NLR) and Platelet Lymphocyte Ratio (PLR) as A Predictor of Mortality in COVİD-19 Riya Sunil; Preethi Chikkanayakanahalli Ramesh; Ashwini Hosahalli Nagaraj; Krupa Kadarappa
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Coronavirus-19 pandemic has stricken our world since December 2019; the disease, first reported in China, is now a pandemic. More than 400 million people have been affected, and 5 million people have succumbed to the disease. Hence, it is the need of the era to find readily available laboratory parameters to assess the mortality chances in these patients. Our study aims to determine the utility of NLR and PLR ratios as a predictor of severity and clinical outcome of COVID-19 patients.100 patients admitted to a tertiary care hospital in Karnataka, India, during the months April to July 2020 were studied. Only patients with a positive RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) report for COVID-19 were included. Demographic data, comorbidities, and mortality status were collected from electronic hospital records. Lab parameters including- Total Count (TC), Absolute count of neutrophils and lymphocytes, platelet count were taken. NLR and PLR were derived from available lab parameters. Patients were categorized into varying severity depending on their SpO2 levels at admission. Neutrophil count (P=0.001) and NLR (P=0.002) were associated with an increased risk of mortality and disease severity. An increase in PLR ratio (P=0.05) shows a mild association with mortality but not with disease severity (P=0.096). In contrast, comorbidities, increasing age, and gender did not show any statistical significance for mortality. The presence of statistical significance concerning NLR and PLR should be utilized as an aid by clinicians to assess disease severity and chances of mortality. As new variants of the disease are uprising and a single therapeutic measure is not available currently for the treatment of COVID-19, clinicians should be well informed about how to monitor the disease in a cost-effective and easily accessible way to reduce the disease mortality and morbidity.

Assessment of the RIPASA Score in the Diagnosis of Acute Appendicitis in Khartoum State Hospitals, Sudan Mohammed Hashim Yousif Ahmed; Aamir Abdallah Hamza; Alawia Mohammed Mansour; Khalid Yousif Abuaagla
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Appendectomy is a common cause of emergency hospitalization, and appendectomy is one of the most common emergency procedures performed in modern medicine. Although it is a common problem, acute appendicitis remains a complex condition to diagnose, especially in the young, the elderly, and women of childbearing age. The aim was to evaluate the accuracy of the RIPASA score for patients with right iliac fossa pain (RIF) and suspected appendicitis. This study is an observational, analytic, and prospective cohort study conducted for one year. All patients suspected of having acute appendicitis and who underwent appendectomy were included in this study. All resected appendages were sent for histological examination. RIPASA scores were calculated for all patients. The study results in the 105 patients studied, 60 (57.1%) were predominantly male. The M/F ratio is 1.3/1. Most of the 98 patients (93.3%) were 40 years old. Most patients had symptoms within 48 hours 79 (75.2%). The most common symptom was anorexia (83.8%). All patients had RIF tenderness (100%). 95 (90.5%) patients had a score >7.5. On gross examination, three patients (2.9%) had a non-inflammatory appendix, 83 patients (79.0%) had a simple inflammatory appendix, the perforated appendix was found in 9 patients (8.6%), appendix mass was found in 6 patients (5,7%), and only four patients (3.8%) had an appendiceal abscess. The appendix was histologically inflamed in 69 patients (65.7%). The sensitivity of the RIPASA scoring system in our study was 69.5%, and the specificity was 70%. The diagnostic accuracy was calculated and was 69.5%. Our study's positive and negative predictive values (PPV, NPV) were 95.7% and 19.4%, respectively. Research conclusion RIPASA value of 7.5 is beneficial and easy to use. Therefore, surgeons can use it as a diagnostic guide for acute appendicitis to reduce the rate of misdiagnosis.

Clinicopathological Study of Mucormycosis in Post Covid Patients, an Epidemic in Pandemic Divya Pujari Jayakeerthy; Pradeep Kumar Reddy Mereddy; Adicherla Govardhan; Seema Bijjaragi
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Mucormycosis is an angio-invasive disease caused by saprophytic fungi of the order Mucorales. Mucormycosis has been established and recognized as a complication of the SARS-CoV-2 infection. Mucormycosis, well known as "black fungus" is creating an epidemic within a global pandemic. The use of different dose regimens, prominent steroids, and multiple comorbidities like Diabetes Mellitus and post-COVID could predispose to mucormycosis. The aim of the study was to study the increase in cases and morphological features of mucormycosis infection in hospitalized post-COVID patients. This prospective study was done for four months, from May 1st, 2021, to August 31st, 2021. All the suspected cases of mucormycosis specimens sent to the Department of Pathology, J.J.M. Medical College, Davangere, for histopathological diagnosis were considered. For microscopic examination, the collected samples were fixed in 10% formalin solution, processed, and stained with Haematoxylin and Eosin. Periodic Acid-Schiff special staining was done. Thirty cases of mucormycosis were collected from patients who had recovered from COVID-19. Mean age 63±12 (37-80) years with Male:Female ratio being 1.3:1. The mean duration between diagnosis of COVID-19 and development of symptoms of mucor was 23.5±10 (7–60) days. Rhino-orbital mucormycosis was the most typical presentation. Out of 30 cases, 28 cases (93.3%) were Type 2 Diabetes Mellitus, and 2 cases (6.6%)were nondiabetic. All patients received corticosteroids for the treatment of COVID-19. Trinity of COVID -19, Diabetes Mellitus, and Corticosteroid treatment was significant for the rise in mucormycosis cases. Mucormycosis is an invasive fungal infection and had seen a sudden surge during the second wave of COVID-19. In our study, most patients had a history of diabetes, and corticosteroid therapy with rhino-orbital mucormycosis was the most typical presentation. As the severity of the necrosis increases, the presence of fungal hyphae also increases, which leads to extensive destruction of tissue. Extra vigilance in immunosuppressed patients helps in reducing the morbidity and mortality due to mucormycosis in Post COVID era.

Effectiveness of Xylene and Mineral Oil in DNA Extraction from Formalin-fixed Paraffin-embedded Diffuse Astrocytic Tumor Edward Jaya Hadi; Hasrayati Agustina; Hermin Aminah Usman
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Formalin-fixed paraffin-embedded (FFPE) tissue is an important source of material for molecular analysis. In the anatomical pathology field, molecular testing is needed in certain tumors, such as astrocytic tumors, to confirm the diagnosis. Extracting DNA from FFPE material is still challenging. The first important step in the extraction process is deparaffinization. This study aims to compare two types of deparaffinization methods. The first method used xylene, and the second one used mineral oil. The results of this study can be used to develop a reliable protocol for DNA extraction from FFPE tissue. DNA from 28 FFPE diffuse astrocytic tumor tissue blocks was extracted. The quantity and A260/A280 ratio of the DNA was measured by spectrophotometer. PCR assays were performed to assess the suitability of extracted DNA for molecular analysis. The results showed that the xylene group has significantly higher DNA concentrations than the mineral oil group (p<0.01). In both groups, average and median A260/A280 scores were between 1.8-2.0. In PCR assay, both groups show a similar result (27 of 28 samples were successfully amplified). In conclusion, xylene and mineral oil can be used effectively as deparaffinization agents. Both methods generated good quality extracted DNA. The advantage of mineral oil is that it is non-toxic and has shorter hands-on time. Xylene is preferable for a small-sized sample because it produces a higher DNA yield.

Significance of D-dimer in Assessment of Severity and Mortality of COVID-19 Patients A Tertiary Care Hospital Study Ashwini Suruthy; Neethu Gowdru Vishwanathan; Seema Bijjaragi
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

The outbreak of COVID-19 has brought public health emergency worldwide and has been the biggest challenge to date. This study aims to study the correlation between D-dimer levels with severity and mortality in COVID-19 patients. This study will help us understand the disease's progression and monitor the disease severity and mortality in Covid-19 patients. This retrospective descriptive study was conducted over four months from April 2021- to June 2021 in a tertiary care hospital in Central Karnataka. Demographic details and clinical and laboratory parameters were obtained from electronic hospital records. Inclusion criteria are Patients who were RT-PCR confirmed positive cases were only included in this study. Exclusion criteria are Patients who had cancer, hematologic malignancies, and acute coronary syndrome were all excluded. Results of the research are A total of 75 cases were evaluated and analyzed in this study. Patients were categorized based on severity as mild to moderate and severe. Out of which 50 patients had recovered from the illness, and 25 patients have succumbed to death. Our study revealed that elevated D-dimer levels were significant (p<0.02) with the outcome of the disease, and with the mean level of 929.27 in severe patients and 466.47 in mild-moderate patients, elevated D-dimer were highly significant (p<0.001) with the severity of the disease. Conclusion research detailed investigations of 75 COVID-19 patients suggests that a significant increase in D-dimer levels was associated with mortality and severity of the disease. Therefore, we strongly recommend that the D-dimer be used to screen patients with COVID-19 to evaluate the severity and predict the prognosis and mortality in hospitalized COVID-19 patients during admission and follow-up throughout hospitalization.

Screening of Sengkuang (Dracontomelon dao (Blanco) Merr. & Rofe) as Antibacterial Escherichia coli Ratih Dewi Dwiyanti; Nurlailah Nurlailah
Medical Laboratory Technology Journal Vol. 8 No. 1 (2022): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Dracontomelon dao (Blanco) Merr. & Rofe has antimicrobial potential, but Dracontomelon dao (Blanco) Merr. & Rofe, originating from Kalimantan, Indonesia, has not been tested for its antibacterial properties and secondary metabolite content. This study aimed to analyze the secondary metabolite content of various Dracontomelon dao (Blanco) Merr leaf extracts. & Rofe and their antibacterial effectiveness against Escherichia coli. The study used 96% ethanol extract, ethyl acetate, petroleum ether, and chloroform by maceration simplicia leaves. Dracontomelon dao (Blanco) Merr. & Rofe is from South Kalimantan, Indonesia. Antibacterial testing was carried out using the agar diffusion method, the Minimum Inhibitory Concentration (MIC) method, and the Minimum Bactericidal Concentration (MBC) method. The results showed that the triterpenoid content was the highest in all extracts, namely ethanol extract 669.8 ± 2,000 mg/ml, ethyl acetate 90 ± 7,638 mg/ml, petroleum ether 792, 800 ± 4,583 mg/ml, chloroform 615, 467 ± 0,577 mg/ml. ml. The antibacterial test using the well diffusion method showed the best inhibition of bacterial growth in ethanol extract, with the largest inhibition zone of 24 mm at a concentration of 500 mg/ml. The study's conclusion was the extract of ethanol, ethyl acetate, petroleum ether and chloroform from the leaves of Dracontomelon dao (Blanco) Merr. & Rofe contains the same five secondary metabolites: flavonoids, alkaloids, saponins, tannins and triterpenoids. All types of extracts showed varying abilities to inhibit the growth of Escherichia coli. Further research is needed on the antibacterial properties of Dracontomelon dao (Blanco) Merr extract. & Rofe, especially on the active substance triterpenoid, which is the largest content in all types of extracts.

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Ricko Dharmadi Utama

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Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Ricko Dharmadi Utama

Contact Email apinaga53@gmail.com

Phone +6282256602225

Journal Mail Official editormltj@gmail.com

Editorial Address Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location Kota banjarmasin, Kalimantan selatan INDONESIA

Abstract

Abstract

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Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ricko Dharmadi Utama

Contact Email
apinaga53@gmail.com

Phone
+6282256602225

Journal Mail Official
editormltj@gmail.com

Editorial Address
Jl. H. Mistar Cokrokusumo No.1A, Kemuning, Kec. Banjarbaru Selatan, Kota Banjar Baru, Kalimantan Selatan 70714

Location
Kota banjarmasin,

Kalimantan selatan

INDONESIA