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Cytopathology Lymphadenopathy Feature in HIV Positve Patient: Diagnosis Tools Comorbidities Agustina, Hasrayati; Wisudarma, Yenni; Kristiana, Ris; Hernowo, Bethy S.
Journal of Medicine and Health Vol 1, No 3 (2016)
Publisher : Maranatha Christian University

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Lymphadenopathy is enlarged lymph nodes caused by infection, inflammation or malignancy. On HIV positive patients, lymphadenopathy is one of the most common clinical manifestations and it is usually persistent. Fine-needle aspiration biopsy (FNAB) is an effective cytology technique in determining the diagnosis of lymphadenopathy. This study aimed to describe the cytopathology of lymphadenopathy in HIV positive patients. This is a descriptive study of 21 cases of lymphadenopathy in patients with HIV positive who underwent FNAB examination in Anatomical Pathology Department of Dr.Hasan Sadikin Hospital between 2013-2014. Medical data was taken from the patient medical records including age, sex, location, size and cytopathological diagnosis. Cytopathology overview of FNAB specimens were reassessed by 2 pathologists. In this study, lymphadenopathy in HIV positive patients were mainly found in men (n = 15.71%) with an average age between 20-30 years. The most frequent location was the neck (n = 20.95.2%). The lymph nodes size were found between 0.5-3 cm. Most diagnosis was tuberculous lymphadenitis (n = 15.71%) with the most common cytology feature was granulomatous lymphadenitis (n = 5.33.3%) and suppurative lymphadenitis (n = 5.33.3%). FNAB examination in lymphadenopathy is very helpful to identify the cause of infection in HIV positive patients. Keywords: FNAB, HIV, lymphadenopathy, cytopathology

Analysis of Fibronectin and TGF-Î²1 Immunoexpression to Determination of Wound Vitality in Wistar Rats (Rattus norvegicus) Novita, Nita; Agustina, Hasrayati; Hernowo, Bethy S.; Hassan, Abdul H.
Journal of Medicine and Health Vol 1, No 1 (2015)
Publisher : Maranatha Christian University

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Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-Î²1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design.Â The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-Î²1 (100%).Â In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-Î²1.Â In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-Î²1.Â In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-Î²1.Â In postmortem wound, all of samples showed negativity for fibronectin and TGF-Î²1. In conclusion, fibronectin and TGF-Î²1 may be useful in the determination of wound vitality.Â Keywords: wound, fibronectin, TGF-Î²1, vitality

Characteristics of Thyroiditis Patients in Dr. Hasan Sadikin General Hospital in 2009â2013 Maryanti, Sri; Agustina, Hasrayati; Mifatuhrachman, Mifatuhrachman
Althea Medical Journal Vol 3, No 1 (2016)
Publisher : Althea Medical Journal

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Background: It is reported that thyroid diseases affect around 200 milion people in the world. One of them is thyroiditis that may cause the risk of cancer. Moreover, thyroiditis can also cause hormonal disorders, such as hypothyroid and hyperthyroid. It is assumed that thyroiditis has distinctive clinical characteristics. The aim of this study was to evaluate the characteristics of thyroiditis based on age, gender, location, and clinical features of the patient.Methods: The study was conducted using descriptive-retrospective method. The data were collected from patientsâ medical records through total sampling from January 2009 to Desember 2013 in Dr. Hasan Sadikin General Hospital, Bandung.Results: It was found 35 cases of thyroiditis. Based on the histopathological type, the most frequently found thyroiditis was Hashimotoâs thyroiditis. Based on age, thyroiditis mostly affected people at age 41â60 years old. Based on the gender, thyroiditis mostly affected female and the location of lesions were bilateral. The majority clinical features of patients were hypothyroid in Hashimotoâs thyroiditis, hyperthyroid in acute thyroiditis, and normothyroid in sub-acute granulomatous thyroiditis.Conclusions: Based on age, gender, and the location, the majority type of thyroiditis did not show specific characteristics. However, thyroiditis showed specific characteristics based on the clinical features of patient. [AMJ.2016;3(1):49â53]DOI:Â 10.15850/amj.v3n1.701

Association between Histopathological Grading and Clinical Staging in Nasopharyngeal Carcinoma Naomi, Sonya M; Dewi, Yussy A; Agustina, Hasrayati
Journal of Medicine and Health Vol. 2 No. 2 (2018)
Publisher : Universitas Kristen Maranatha

Nasopharyngeal Carcinoma (NPC) is the most common cancer in head and neck. Grading of NPC is based on keratinization, growth, and cell junction, and it can be classified into well differentiated, moderately differentiated, poorly differentiated, and undifferentiated. On the other hand, the staging of NPC based on UICC/AJCC staging system is divided into Stage I-IVC. This classification is based on TNM that is related to the ability of cancer cells to invade normal cells. This study was conducted from May to June 2017 in Oncology Clinic of Otorhinolaryngology-Head and Neck Surgery Department and Pathology Anatomy Department of Hasan Sadikin General Hospital. This is an analytical correlative study with cross sectional design. The data is collected retrospectively from medical records of patients who are diagnosed as NPC with adequate grading and staging data. Incomplete medical records, multiple carcinoma, and recurrent carcinoma are excluded from this study. We find out that among 51 samples, predominant is Stage IVB NPC (37.3%) and undifferentiated grade NPC (76.5%). Statistical analysis shows that there is no significant association between grading and staging (r=0.275; p=0.159).This study concludes that there is no association between histopathological grading and clinical staging in nasopharyngeal carcinoma.Keywords: nasopharyngeal carcinoma, grading, staging

Analysis of Fibronectin and TGF-Î²1 Immunoexpression to Determination of Wound Vitality in Wistar Rats (Rattus norvegicus) Novita, Nita; Agustina, Hasrayati; Hernowo, Bethy S.; Hassan, Abdul H.
Journal of Medicine and Health Vol. 1 No. 1 (2015)
Publisher : Universitas Kristen Maranatha

Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-Î²1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design.Â The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-Î²1 (100%).Â In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-Î²1.Â In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-Î²1.Â In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-Î²1.Â In postmortem wound, all of samples showed negativity for fibronectin and TGF-Î²1. In conclusion, fibronectin and TGF-Î²1 may be useful in the determination of wound vitality.Â Keywords: wound, fibronectin, TGF-Î²1, vitality

Cytopathology Lymphadenopathy Feature in HIV Positve Patient: Diagnosis Tools Comorbidities Agustina, Hasrayati; Wisudarma, Yenni; Kristiana, Ris; Hernowo, Bethy S.
Journal of Medicine and Health Vol. 1 No. 3 (2016)
Publisher : Universitas Kristen Maranatha

Lymphadenopathy is enlarged lymph nodes caused by infection, inflammation or malignancy. On HIV positive patients, lymphadenopathy is one of the most common clinical manifestations and it is usually persistent. Fine-needle aspiration biopsy (FNAB) is an effective cytology technique in determining the diagnosis of lymphadenopathy. This study aimed to describe the cytopathology of lymphadenopathy in HIV positive patients. This is a descriptive study of 21 cases of lymphadenopathy in patients with HIV positive who underwent FNAB examination in Anatomical Pathology Department of Dr.Hasan Sadikin Hospital between 2013-2014. Medical data was taken from the patient medical records including age, sex, location, size and cytopathological diagnosis. Cytopathology overview of FNAB specimens were reassessed by 2 pathologists. In this study, lymphadenopathy in HIV positive patients were mainly found in men (n = 15.71%) with an average age between 20-30 years. The most frequent location was the neck (n = 20.95.2%). The lymph nodes size were found between 0.5-3 cm. Most diagnosis was tuberculous lymphadenitis (n = 15.71%) with the most common cytology feature was granulomatous lymphadenitis (n = 5.33.3%) and suppurative lymphadenitis (n = 5.33.3%). FNAB examination in lymphadenopathy is very helpful to identify the cause of infection in HIV positive patients. Keywords: FNAB, HIV, lymphadenopathy, cytopathology

Characteristics of Mediastinal Tumors in Dr. Hasan Sadikin General Hospital, Periods of 2011-2016 Giovani, Octavianus; Agustina, Hasrayati; Djajakusumah, Teguh M
Journal of Medicine and Health Vol. 2 No. 2 (2018)
Publisher : Universitas Kristen Maranatha

Mediastinal tumors are considered to be mediastinal disease with various clinical and histopathologic presentations. In Indonesia, literatures concerning mediastinal tumors are still few. This study aims to obtain the characteristic of mediastinal tumors in Dr.Hasan Sadikin Hospital. A cross-sectional retrospective study was conducted using medical records from 2011 to 2016. All medical records with clinical diagnosis of mediastinal tumors are included, and cases with metastatic tumors from distant sites to the mediastinum, and/or incorrect histopathologic findings are excluded. We obtain data of age, gender, clinical symptoms, histopathologic finding, and staging, with total samples collected of 72. We find out that patients with mediastinal tumors are mainly over 40 years old (62.5%), especially in the 6th decade of life (23.6%), and were mostly of males (73.6%). Patients mainly had chief complaints of dyspnea (38.7%), followed by cough and typical myasthenia gravis symptoms. The most common type of mediastinal tumor is epithelial cell tumor (68.4%), and the most common stage is Masaoka-Koga stage III (37.5%). This study concludes that mediastinal tumor mainly occurs in males aged 40 years old and above, with epithelial cell tumor as the most common histopathologic findings.Keywords : characteristics; mediastinal tumor; mediastinum

THE IMMUNOEXPRESSION PROFILE OF FGFR3 AND P53 IN PUNLMP AND UROTHELIAL BLADDER CARCINOMA AT HASAN SADIKIN GENERAL HOSPITAL Suryanti, Sri; Agustina, Hasrayati; Mulyati, Sri
Indonesian Journal of Urology Vol 27 No 2 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i2.551

Objective: To obtain FGFR3 and p53 immunohistochemistry of PUNLM and UBC basic data profile. Material & Methods: This retrospective observational analytic study used a cross-sectional design on formalin-fixed paraffin-embedded (FFPE) from patients diagnosed as PUNLMP and UBC then performed FGFR3 and p53 immunohistochemical staining. Statistical analysis of immunoexpression data using SPSS 24.0. Results: There are three main immunohistochemistry pattern profiles of PUNLMP and UBC in this research data, i.e. FGFR3+ p53+, FGFR+ p53- and FGFR3- p53+. There were no Cis sample obtained. FGFR+ was showed in PUNLMP, LG-PUC, HG-PUC, IUBC (pT1, pT2, pT3, pT4) with decreased percentage sequentially. As many as 51 of 54(94.44%) IUBC samples showed combination pattern with p53+ while 3 other samples were p53-. Conclusion: The FGFR3 and p53 immunohistochemistry profile, separately or as a pattern of combination, is in accordance with the oncogenesis molecular pathway of UBC.

THE IMMUNOEXPRESSION PROFILE OF FGFR3 AND P53 IN PUNLMP AND UROTHELIAL BLADDER CARCINOMA AT HASAN SADIKIN GENERAL HOSPITAL Suryanti, Sri; Agustina, Hasrayati; Mulyati, Sri
Indonesian Journal of Urology Vol 27 No 2 (2020)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v27i2.551

Objective: To obtain FGFR3 and p53 immunohistochemistry of PUNLM and UBC basic data profile. Material & Methods: This retrospective observational analytic study used a cross-sectional design on formalin-fixed paraffin-embedded (FFPE) from patients diagnosed as PUNLMP and UBC then performed FGFR3 and p53 immunohistochemical staining. Statistical analysis of immunoexpression data using SPSS 24.0. Results: There are three main immunohistochemistry pattern profiles of PUNLMP and UBC in this research data, i.e. FGFR3+ p53+, FGFR+ p53- and FGFR3- p53+. There were no Cis sample obtained. FGFR+ was showed in PUNLMP, LG-PUC, HG-PUC, IUBC (pT1, pT2, pT3, pT4) with decreased percentage sequentially. As many as 51 of 54(94.44%) IUBC samples showed combination pattern with p53+ while 3 other samples were p53-. Conclusion: The FGFR3 and p53 immunohistochemistry profile, separately or as a pattern of combination, is in accordance with the oncogenesis molecular pathway of UBC.

Korelasi antara Imunoekspresi LMP-1 Virus Epstein-Barr dengan Respon Kemoterapi CHOP pada Limfoma Maligna Non-Hodgkin Tipe Diffuse Large B Cell. Inas Susanti; Hasrayati Agustina; Bethy S Hernowo
Majalah Patologi Indonesia Vol 23 No 2 (2014): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

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Background Diffuse large B cell lymphoma is the most common type of B-cell non-Hodgkin malignant lymphoma. Epstein-Barr virus (EBV) has been shown to contribute to the development of this tumor especially in the elderly patient (above 50 years). The chemotherapeutic agents CHOP (Cyclophospamide, Hydroxydaunorubicin, Oncovin, Prednison) are standard treatment for non-Hodgkin lymphoma patients, but approximately 30% cases shows non-respone with standard chemotherapy and undergoes progressive disease until death. There are no accurate predictor factors in predicting CHOP chemotherapy respone in patients with DLBCL. The aim of this study is to determine the role of LMP-1 in predicting CHOP chemotherapy respone in patients with DLBCL. Methods This study was done retrospectively in 34 cases of DLBCL. The samples were obtained from the Anatomic Pathology Department of Dr. Hasan Sadikin Hospital from January 2009 to Desember 2011. All cases were reviewed for histopathological diagnoses based on the WHO Classification of 2001 and the specimens were stained with specific antibodies against CD 20 (for diagnoses DLBCL) and LMP-1. The data were analized using rank Spearmanâ€™s correlation test. Results This study showed that 31 cases (91.18%) were LMP-1 positive, only 3 cases (8.82%) were negative. Three cases of negative LMP-1 had a good respone to chemotherapy, whereas among 31 positive cases LMP-1 showed good respone to chemotherapy in 21 cases and the remaining 10 cases had a poor respone to chemotherapy. There was no correlation between EBV-LMP-1 immunoexpression with CHOP chemotherapy respone in DLBCL (p=0.436). Conclusion There was no correlation between EBV-LMP-1 immunoexpression with CHOP chemotherapy respone in DLBCL. Key words: CHOP, DLBCL, EBV, LMP-1, non-Hodgkin lymphoma.

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