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Articles 15 Documents
Search results for , issue "Vol 10 No 2 (2025): November" : 15 Documents clear
Bioprospecting of Potential Microorganisms as Agents for Biodiesel Production from Sago Pulp Waste Feedstock Lethulur, Alice Chaela; Seumahu, Cecilia Anna; Apituley, Edwin Thomas
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.48431

Abstract

The rising global energy demand and environmental issues associated with fossil fuel use have accelerated interest in renewable alternatives, such as biodiesel. This study aimed to provide a preliminary screening of potential indigenous microorganisms among isolates with high ability to saccharify lignocellulosic sago pulp waste, an abundant, non-food biomass in Maluku, and to accumulate lipids. Laboratory experiments were conducted to isolate, characterize (macro- and microscopically), saccharify, and accumulate lipids. Bacterial isolates were evaluated for saccharification ability using two parameters: the cellulolytic index (CI), determined by the clear zone on solid CMC media, and reducing sugar production in modified liquid CMC media using pretreated sago waste, measured by Benedict’s test. Yeast and mold isolates were assessed for lipid accumulation through Sudan Black B staining and gravimetric lipid extraction under nitrogen-limited conditions. Results identified four bacterial isolates capable of saccharification, with isolate BAS 1B showing the highest cellulolytic index, and all isolates produced detectable reducing sugars by Benedict’s test. All yeast and mold isolates accumulated intracellular lipids, with YAS 2 and KAS 2 isolates accumulating the highest lipid compared to the positive control (Saccharomyces cerevisiae). As this study was based on a single-point observation (n=1), the results are exploratory and should be interpreted cautiously. Nevertheless, isolates BAS 1B, YAS 2, and KAS 2 show promising potential for further biodiesel-related research using lignocellulosic waste. Further studies with replication and quantitative validation are required prior to any industrial consideration
Assessing the Ecological Status of the Curug Tilu Leuwi Opat Waterfall Using Macroinvertebrates and Physicochemical Parameters Hanif Syafrian Purnama; Tati Suryati Syamsudin; Dian Rosleine
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49080

Abstract

Anthropogenic activities increasingly threaten freshwater availability. The Curug Tilu Leuwi Opat waterfall in the Cimahi River, an important water source for surrounding communities and a popular tourist site, requires an ecological assessment to support effective water management. This study aims to provide an initial overview of the ecological status of waters through an integrative approach that combines benthic macroinvertebrates and physicochemical parameters. Sampling was conducted in two waterfall streams, Kacapi and Aseupan, which are part of the Curug Tilu Leuwi Opat waterfall system. Physicochemical parameters measured included temperature, flow velocity, pH, dissolved oxygen, conductivity, and total dissolved solids. Benthic macroinvertebrates were collected using a Surber net and identified to the lowest feasible taxonomic level, typically morphospecies with some taxa at the family level, and subsequently analyzed using the Shannon–Wiener index, Simpson’s dominance, the Family Biotic Index (FBI), and Canonical Correspondence Analysis. Results indicated excellent water quality (FBI at Kacapi = 4.17; Aseupan = 4.24), driven by the presence of pollution-sensitive taxa from Leptophlebiidae, Tipulidae, and Perlidae, while the slightly higher FBI at Aseupan reflected several moderately tolerant families such as Physidae and Simuliidae. Benthic macroinvertebrate diversity was moderate with no dominant species, indicating stable community structure. Most measured parameters that met established standards met Class I criteria, while dissolved oxygen, conductivity, and flow velocity emerged as the dominant variables shaping community structure. Overall, the aquatic ecological status was excellent, highlighting the role of aquatic biodiversity as a scientific basis for sustainable environmental management
The Effect of IAA, BAP, and Coconut Water on Tomato (Solanum lycopersicum L.) Organogenesis Alba Wijaya Maharani; Restiani, Ratih; Aniek Prasetyaningsih
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49410

Abstract

Tomato (Solanum lycopersicum L.) is a widely cultivated horticultural crop valued for its phytochemical and nutritional content. The increasing demand for high-quality seedlings has promoted the use of in vitro propagation, which offers greater efficiency in labor and land use, uniform seedling production, and independence from climate compared to conventional methods. This study aimed to investigate the effect of different concentrations of plant growth regulators (PGRs) and coconut water as an alternative or supplement on the organogenesis of tomato hypocotyl explants. Tomato hypocotyl explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP (0.5, 1.0, and 1.5 ppm), IAA (0.1, 0.3, and 0.5 ppm), and coconut water (10%, 20%, and 30%). The experiment was arranged in a Completely Randomized Design (CRD) with four replications, consisting of a control, single-factor treatments, and combination treatments of BAP, IAA, and coconut water. Observations included the percentage and intensity of root, shoot, and callus formation, as well as shoot height. Data were analyzed using the non-parametric Kruskal–Wallis test, followed by Dunn’s test. The results showed that roots, shoots, and callus successfully developed in treatments supplemented with 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA), and coconut water. The highest shoot growth, with a percentage of 87.7% and an average height of 5.75 cm, was observed in treatment K20, while the highest callus formation occurred in treatment K30. The best shoot and callus intensities were resulted in media with coconut water alone at concentrations of 10%, 20%, and 30%, whereas the highest root intensity was obtained in the BAP+IAA treatment without coconut water. The results indicate that coconut water is effective in supporting organogenesis, both directly through the formation of shoots and roots, and indirectly through callus formation that can subsequently develop into shoots and roots.
Etnobotanical Study of Medicinal Plants by the Community Pucung Village, Kismantoro District, Wonogiri Regency, Central Java Nur Kholis Majid, Muhammad; Agustina, Ana; Lasekti Wicaksono, Rezky
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49761

Abstract

Indonesia is known as a living laboratory because its forests host approximately 30,000 plant species, of which around 9,600 are medicinal. The benefits of natural medicines derived from plants have been empirically proven, encouraging communities to preserve them. The use of medicinal plants by communities reflects the relationship between humans and plants, known as ethnobotany. This relationship can show how the community of Pucung Village uses medicinal plants, the efforts and conservation status of the medicinal plant species found, and the community's perceptions of medicinal plants and the sustainability of local knowledge about their use for future generations. Using an ethnobotanical approach, this study focuses on local wisdom as the foundation for preserving knowledge of medicinal plants in Pucung Village. The data collection methods used were purposive sampling and snowball sampling. Using these techniques, 18 informants were identified. Based on the interview results, 56 plant types from 30 families were identified, with turmeric (100%) being the most frequently used. The plant part with the highest percentage was leaves (45.16%). The most dominant processing method was boiling (37.70%). The primary source of plant acquisition was cultivation activities (49.30%). The most common plant habit was herbaceous (37%). There are 24 medicinal plant species classified as least concern, 9 as data deficient, and 23 as not evaluated. Efforts to sustain local knowledge include direct practice and the transmission of knowledge through oral communication. Nine informants tend to use medicinal plants to maintain physical health, while nine others tend to prefer using medicinal plants first.
Detection of Porcine Contamination in Beef Meatballs from Gedangan District Using PCR–RFLP Suryadi, Gian Firdaus Putra; Astuti, Rahma Windi; Fitriani, Lilis Nur; Lisdiana, Lisa
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.49813

Abstract

Meatball is one of the popular meat dishes in Indonesia, especially in Sidoarjo. The sale of meatballs in Sidoarjo is carried out by Micro, Small, and Medium Enterprises (MSMEs), with 330 of the total 11,114 micro-traders selling meatballs. However, there is a risk of pork being adulterated into meatballs. This is a serious problem because Indonesia, especially Sidoarjo, is a Muslim-majority city with 95.5% of the total population of 2 million. Therefore, the purpose of this article is to conduct field checks to determine whether or not pork contamination is present in meatballs in Sidoarjo. Sampling locations were determined using a stratified random sampling method, which found that eight of the 15 villages, including Karangbong, Tebel, Sruni, Keboananom, Keboansikep, Gedangan, Ketajen, and Sawotratap Villages, have a significance value greater than the alpha value (0.05). Therefore, eight of them were used as sampling locations. One sample was purchased from each of the eight chosen villages, named S1, S2, S3, S4, S5, S6, S7, and S8. Meanwhile, the possibility of porcine contamination in beef meatball samples was analyzed using the Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method with the restriction enzyme BsaJI. This study found that all eight samples and the negative control DNA amplicons were not cleaved by the BsaJI enzyme. Meanwhile, the positive control DNA amplicon was cleaved into 3 fragments. It was indicated that all samples and the negative control DNA amplicons were free of pork DNA contamination. Despite the anomaly, it can be concluded that all meatball samples bought from Gedangan District are not contaminated with pork. By demonstrating that meatballs from Sidoarjo are free from pork DNA, this study serves as an additional halal food monitoring in Sidoarjo. It reinforces public trust in local MSME food products.

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