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INDONESIA
Molecular and Cellular Biomedical Sciences (MCBS)
Published by Cell and Biopharmaceutical Institute
ISSN : 25274384     EISSN : 25273442     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Medicine & Pharmacology Neuroscience
Molecular and Cellular Biomedical Sciences (MCBS) has been published by Cell and BioPharmaceutical Institute (CBPI), a biannually published scientific journal, is an open access, peer-reviewed journal that supports all topics in Biology, Pathology, Pharmacology, Biochemistry, Histology and Biomedicine in the aspect of molecular and cellular.
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Articles 7 Documents
 1 
Search results for , issue "Vol 9, No 2 (2025)" : 7 Documents clear
Hypoxia-Induced Mesenchymal Stem Cell Exosomes Modulate Protein Kinase A and VEGFR Expression in Ultraviolet B-Induced Hyperpigmentation in Mice Andavania, Sheila Jessica; Syamsunarno, Mas Rizky; Putra, Agung; Setiawan, Eko
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.594

Abstract

Background: Hyperpigmentation is often exacerbated by ultraviolet-B (UVB) exposure through oxidative stress and activation of pathways like mitogen-activated protein kinase (MAPK) and vascular endothelial growth factor receptor (VEGFR). Current treatments carry risks and necessitate safer alternatives. This study investigated the therapeutic potential of hypoxia-induced mesenchymal stem cell (MSC) exosomes in reducing protein kinase-A (PKA) and VEGFR expression in UVB-induced hyperpigmentation.Materials and methods: A post-test-only control group design was used with 30 male C57BL/6 mice divided into five groups: Healthy group, 0,9% NaCl-treated group, retinol-treated group, and two treatment groups (200 µL Exosomes-treated group and 300 µL Exosomes-treated group. UVB-induced hyperpigmentation was established with 180 mJ/cm² exposures over two weeks. Treatment was administered via subcutaneous injections for seven days. PKA and VEGFR mRNA levels were analyzed using qRT-PCR.Results: PKA expression was significantly lower in the 200 µL Exosomes-treated group (0.34±0.05) and 300 µL Exosomes-treated group (0.21±0.04) groups compared with the 0,9% NaCl-treated group (1.12±0.08) (p<0.001). VEGFR expression similarly decreased in 200 µL Exosomes-treated group (0.32±0.05) and 300 µL Exosomes-treated group (0.18±0.04) versus the 0,9% NaCl-treated group (1.48±0.09) (p<0.001). Both exosome doses achieved reductions comparable to baseline levels observed in the Healthy group.Conclusion: Hypoxia-induced MSC exosomes reduced PKA and VEGFR expression in UVB-induced hyperpigmentation, with the 300 µL dose showing greater efficacy. These findings suggested exosome therapy as a promising alternative for hyperpigmentation treatment. Keywords: hyperpigmentation, MSC, PKA, VEGFR, melanin
Slow 0.9% NaCl Bolus Administration Reduces ANP, MMP-2, and Syndecan-1 Shedding in Septic Shock Rabbit Models Hartawan, I Nyoman Budi; Wiryana, Made; Jawi, I Made; Astawa, I Nyoman Mantik; Bakta, I Made; Subanada, Ida Bagus; Suparyatha, Ida Bagus; Wati, Dyah Kanya
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.590

Abstract

Background: The optimal rate for fluid bolus administration in septic shock remains a critical and unresolved question. Rapid bolus administration is commonly practiced but has been linked to elevated levels of atrial natriuretic peptide (ANP), matrix metalloproteinase-2 (MMP-2), and syndecan-1 shedding, potentially exacerbating endothelial glycocalyx damage and increasing vascular permeability. However, the physiological and clinical implications of slower bolus rates have not been thoroughly investigated. This study was conducted to identify safer fluid management practices and improve patient outcomes in septic shock.Materials and methods: A randomized post-test-only control group design was employed, involving 36 male New Zealand rabbits with lipopolysaccharide-induced septic shock. The treatment group received 0.9% NaCl boluses (20 mL/kg body weight) over 20 minutes per bolus (slow bolus), while the control group received the same volume over 5 minutes per bolus (rapid bolus). ANP, MMP-2, and syndecan-1 levels were measured using ELISA 10-15 minutes post-intervention.Results: The median ANP levels in the treatment group (92.86 ng/mL) were significantly lower (p<0.05) than those in the control group (367.32 ng/mL). The mean MMP-2 levels in the treatment group (10.26 ng/dL) were lower than those in the control group (11.43 ng/dL). The median levels of syndecan-1 were also lower in the treatment group (4.31 ng/mL) compared to the control group (5.94 ng/mL).Conclusion: Slow fluid boluses appear to mitigate endothelial damage by reducing ANP, MMP-2, and syndecan-1 shedding. These findings suggest that slower infusion rates may offer a protective advantage in fluid resuscitation, paving the way for updated clinical guidelines.Keywords: fluid bolus, ANP, MMP-2, syndecan-1
1’- Acetoxychavicol acetate Suppresses Osteosarcoma Cell Proliferation through the PI3K Pathway: A Molecular Docking and Cytotoxicity Study Safitri, Putri Gita Ayu; Da’i, Muhammad; Wulandari, Febri
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.640

Abstract

Background: This study aims to investigate anticancer properties of 1'-Acetoxychavicol acetate (ACA), a phenylpropanoid substance obtained from the rhizomes of the Alpinia genus, which has been extensively studied. However research on its cytotoxic effects, particularly against osteosarcoma cells, has never been donenot been conducted. The purpose of the research is to investigate the anticancer potential of ACA to support the its development as a novel therapeutic candidate.Materials and methods: This study assessed ACA’s initial anticancer potential through in vitro cytotoxic tests on normal human osteoblast cells (hFOB) and osteosarcoma cells (MG-63) using the MTT assay. Additionally, bioinformatics analyses, including target prediction, gene ontology, hub gene identification, protein-protein interactions (PPI) network construction, Kyoto encyclopedia of genes and genomes (KEGG) pathways analysis, disease association analysis, and molecular docking, were performed. Results: The cytotoxicity test on normal hFOB showed an IC50 of 45.05 µM, while in MG-63 osteosarcoma cells, the IC50 was 20.41 µM. In the bioinformatics test, top five target genes identified were SRC, GNAI1, PIK3CD, PIK3CB, PIK3R3. Molecular docking analysis showed that, the native PI3KD ligand showed a strong binding affinity of -10.99 kcal/mol and interacted with more amino acid residues.Conclusion: Overall, ACA exhibits promise as a treatment option to inhibit osteosarcoma cell proliferation by targeting the PI3K pathway. To develop ACA as a potential osteosarcoma therapeutic candidate, extensive in vitro research is needed.   Keywords: 1-Acetoxychavicol acetate, bioinformatics, cytotoxicity, osteosarcoma, PI3K pathway
Increased expression of pap and sfa Genes in Biofilm-Forming Uropathogenic Escherichia coli Associated with Urinary Tract Infections Purbowati, Rini; Utami, Sri Lestari
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.589

Abstract

Background: Urinary tract infections cover a broad spectrum of infectious syndromes and affect the urinary tract from the urethra to the kidneys. Generally caused by uropathogenic Escherichia coli (UPEC), and their pathogenesis is greatly influenced by biofilm formation, which results in persistent and recurrent infections. UPEC uses filamentous adhesive structures such as pili or fimbriae, pyelonephritis-associated pili, and S fimbriae, which are regulated by the pap and sfa operons, respectively. The purpose of the study was to detect the effects of two virulence genes, (pap 7 and sfa 9) on biofilm-forming UPEC associated with urinary tract infections.Materials and methods: A total of 123 UPEC isolates were collected from clinical microbiology laboratory section of a general hospital in Surabaya, Indonesia. Urine samples yielded UPEC with significant counts (≥105 CFU/ml), and the biofilm development was analyzed using the Congo red agar method. The presence of pap 7 and sfa 9 genes in the isolates was determined using PCR assay. Results: Among the 123 UPEC isolates, 66 isolates were able to form biofilms, as determined using the Congo red agar (CRA) method. Biofilm-forming UPEC isolates exhibited a high positivity frequency for the pap 7 gene (65.85%), while the positivity frequency for the sfa 9 gene was significantly lower (14.63%).  Conclusion: An increase in th expression of pap 7 and sfa 9 are is associated with the ability to form biofilms, which could serve as a diagnostic marker for biofilm formation potential vaccine target.Keywords: biofilm, pap, sfa, uropathogenic, Escherichia coli, UTI
Mesenchymal Stem Cell-Derived Exosomes Enhance FGF-1 and SDF-1 Expression in Rats with Second Degree Burns Hariani, Nova Putri; Putra, Agung; Subchan, Prasetyowati; Setiawan, Eko
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.635

Abstract

Background: Second-degree burns cause extensive damage to the skin and pose significant health challenges, with current treatments facing limitations such as donor skin shortages and complications. Fibroblast growth factor 1 (FGF-1) and stromal-derived growth factor 1 (SDF-1) are critical for tissue repair. Emerging evidence suggests that mesenchymal stem cell-derived exosomes (E-MSCs) are a promising cell-free therapeutic option for enhancing wound healing through the modulation of FGF-1 and SDF-1. This study investigated the effect of E-MSCs on the expression of FGF-1 and SDF-1 genes in rats with second-degree burns.Materials and methods:  This experimental study used a second-degree burn model in Wistar rats, treated with subcutaneous injections of E-MSCs at doses of 100 µL and 200 µL. Gene expression of FGF-1 and SDF-1 was quantified using qRT-PCR. Histological validation confirmed burn severity, and flow cytometry was used to characterize E-MSCs and exosomes.Results: An increase in FGF-1 and SDF-1 expression was observed in exosome-treated groups compared to the NaCL-treated group. The 200 µL E-MSCs-treated group showed the most significant enhancement in both growth factors, with statistically significant differences (p<0.05). These findings underline the efficacy of E-MSCs in modulating critical genes involved in wound healing.Conclusion: E-MSCs significantly upregulate FGF-1 and SDF-1 expression, promoting tissue repair and regeneration in second-degree burn models. This study highlights the potential of E-MSCs as a non-invasive therapeutic approach.  Keywords: exosomes, FGF-1, mesenchymal stem cells, SDF-1
n-Hexane Fraction of Cucumis melo L. Cultivar Gama Melon Parfum: An in vitro Study in MCF7 and T47D Cells Line Salamah, Rohmi; Wijayanti, Nastiti; Widiyanto, Slamet
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.558

Abstract

Background: Cucumis melo a melon species, typically has a sweet taste. Some cultivars are known for their distinctive bitter flesh due to its higher levels of cucurbitacin. Cucurbitacin is semipolar compound that has anticancer properties. However, the anticancer effects of cucurbitacin from gama melon parfum (GMP) have not been widely studied. The use of n-Hexane as a non-polar solvent in GMP melon fractionation is to dissolve the non-polar parts of the plant. However, Cucurbitacin was found in the n-hexane fraction of Cucurbita pepo L. Therefore, this study will investigate the presence of Cucurbitacin in the n-Hexane fraction and its effects on breast cancer cells T47D and MCF7.Materials and methods: Dry simplicia of GMP melon fruit were macerated using methanol and fractionated using n-hexane. The presence of cucurbitacin was detected using the high-performance liquid chromatography (HPLC) method. Cell cytotoxicity tests were assessed using the MTT assay, with concentrations of 7.8125, 15.625, 31.25, 62.5, and 125 µg/mLResults: Cucurbitacin compounds were detected in the n-hexane fraction at a concentration of 7.6 µg/mL per 10 mg of n-hexane fraction. MCF7 cell viability was lower than that of T47D cells across all concentrations tested. MCF7 cell viability was below 50% at a concentration of 62.5 µg/mL. In contrast, T47D cell viability remained at 100% even at the highest concentration of 125 µg/mL. The IC50 value of MCF7 cells was 43.5 µg/mL.Conclusion: The cucurbitacin content in the n-Hexane fraction was 7.6 µg/mL per 10mg fraction. At this concentration, it moderately inhibits the proliferation of MCF7 cells.Keywords: gama melon parfum, cucurbitacin, HPLC, T47D, MCF7
Endophytic Bacteria in Acalypha indica L. Leaves and Their Antimicrobial Activity Against Staphylococcus aureus and Candida albicans Syafitri, Aini; Fitri, Lenni; Suhartono, Suhartono
Molecular and Cellular Biomedical Sciences Vol 9, No 2 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i2.609

Abstract

Background: The anting-anting plant (Acalypha indica L.) is used in herbal medicine in the treatment of various diseases. The leaf extract of this plant is known for its antimicrobial activity, but the antimicrobial properties of the endophytic bacteria within its leaves have never been reported. This research aims to determine the antimicrobial activity of endophytic bacteria from the leaves of the anting-anting plant. Materials and methods: The isolation of endophytic bacteria was performed using the spread plate method on nutrient agar (NA) media. Following isolation, the bacterial isolates were characterized through macroscopic and microscopic examination, as well as biochemical tests, which included indole production, hydrogen sulfide (H2S) production, motility, Simmons citrate utilization, methyl red-Voges-Proskauer (MR-VP) test, catalase test, and triple sugar iron agar (TSIA) test. Identification of the bacterial isolates was conducted according to Bergey's Manual of Systematic Bacteriology. Additionally, the antimicrobial activity of the isolates was assessed using the diffusion methodResults: Fourteen isolates of anting-anting leaf endophytic bacteria were obtained (coded as BEDA 1 to BEDA 14). The BEDA 5 isolate exhibited the largest inhibitory zone diameter against Staphylococcus aureus (31.48 mm), while BEDA 9 showed a significant inhibitory zone diameter against Candida albicans (17.84 mm). Conclusion: The two isolates (BEDA 5 and BEDA 9) exhibited significant antimicrobial activity, indicating their potential as promising candidates for alternative antimicrobial agents. These results suggest that endophytic bacteria from Acalypha indica may play an essential role in combating antibiotic resistance and in the development of new therapeutic strategies.Keywords: endophytic bacteria, characterization, antimicrobial activity, Acalypha indica

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