Garuda - Garba Rujukan Digital

p-Index From 2020 - 2025

2.042

P-Index

This Author published in this journals

All Journal Buletin Veteriner Udayana Jurnal Veteriner Bioscience Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Molecular and Cellular Biomedical Sciences (MCBS) Animal Production : Indonesian Journal of Animal Production Biota Jurnal Inovasi Pembelajaran Karakter Berkala Penelitian Hayati Prosiding Konferensi Nasional PKM-CSR Berkala Ilmiah Biologi

Slamet Widiyanto

Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 415791

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

Published : 16 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

STRESS ESTIMATION OF PRE-SLAUGHTER AND SLAUGHTERED BY MEANS OF FOURIER TRANSFORM INFRARED SPECTROSCOPY ANALYSIS THROUGH MEASUREMENT OF CORTISOL AND CATECHOLAMINE LEVEL IN FEMALE CATTLE URINE Widiyanto, Slamet; Widiyono, Irkham; Putro, Prabowo Purwono; Astuti, Pudji
ANIMAL PRODUCTION Vol 16, No 3 (2014): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (836.056 KB)

Abstract. This study was aimed to analyze the levels of catecholamine and cortisol as stress indicator by invasive and non-invasive methods. Twelve female cattle PO were used in this study and were collected from slaughter house in Yogyakarta. Catecholamine and cortisol level of urine were measured by Enzyme linked Immunoassay (EIA) method and Fourier Transform Infrared (FTIR), were statistically analyzed to determine the difference between pre slaughter and slaughter conditions. FTIR spectra were analyzed using chemo-metrics software. These results showed that the concentration of urinary cortisol were 2.12±1.68 ng/dl of pre-slaughter and 7.58±3.89 ng/dl of slaughtered respectively. The levels of urinary catecholamine in pre slaughter and slaughter were 3.07±2.05 ng/dl and 4.15±2.68 ng/dl respectively. In FTIR analysis showed the spectral separation between the different quadrants before and during slaughter. The result suggested the correlation between the results of the analysis using the EIA and FTIR spectra. It is assumed that the separation of the FTIR spectrum in line with the increased levels of cortisol catecholamine and samples. It can be concluded that FTIR can be used to analyze the status of stress in animals, especially in cattle. Key words: catecholamine, cortisol, non-invasive, FTIR Abstrak. Penelitian ini bertujuan untuk menganalisis kadar katekolamin dan kortisol sebagai indicator stress secara invasif maupun non invasif dan mengembangkan metode deteksi stress pada sapi. Penelitian menggunakan sampel urin sapi Peranakan Ongole (PO) yang diambil dari rumah potong hewan di Yogyakarta. Analisis kadar katekolamin (CA) dan kortisol (CO) dilakukan dengan menggunakan metode EIA dan FTIR. Data konsentrasi kortisol maupun katekolamin hasil pengukuran menggunakan metode EIA dilakukan analisis statistik untuk mengetahui pengaruh stres terhadap konsentrasi CA dan CO urine. Hasil penelitian ini menunjukan rata-rata konsentrasi kortisol urin adalah 2.12±1.68 ng/dl dalam kondisi prapenyembelihan dan 7,58±3,89 ng/dl pada saat penyembelihan. Sedangkan konsentrasi katekolamin urin pada saat prapenyembelihan dan penyembelihan secara berurutan adalah 3,07±2,05 ng/dl dan 4,15±2,68 ng/dl. Analisis FTIR menggambarkan pemisahan spektral pada quadran berbeda antara sebelum dan saat penyembelihan. Berdasarkan hasil ini menunjukkan korelasi antara hasil analisis dengan menggunakan EIA dan FTIR, baik konsentrasi katekolamin dan kortisol dengan spektra FTIR dari sampel. Perbedaan konsentrasi CA dan CO urin prapenyembelihan dan saat penyembelihan sejelan dengan pemisahan spektra FTIR. Hal ini diyakini bahwa pemisahan spektrum FTIR disebabkan adanya peningkatan kadar katekolamin dan kortisol dari sampel. Dapat disimpulkan bahwa FTIR dapat untuk menganalisis status stres pada hewan khususnya pada sapi. Kata kunci : katekolamin, kortisol, non invasif, FTIR

PENINGKATAN KETERAMPILAN MENGAJAR MELALUI TEKNIK SUPERVISI INDIVIDUAL JENIS OBSERVASI KELAS PADA GURU Widiyanto, Slamet
Jurnal Inovasi Pembelajaran Karakter Vol 4, No 2 (2019): Edisi Desember 2019
Publisher : Jurnal Inovasi Pembelajaran Karakter

Show Abstract | Download Original | Original Source | Check in Google Scholar

Penelitian ini dilatarbelakangi oleh kompetensi kemampuan guru kelas di SD Negeri Kertasari 04 dalam mengajar masih rendah. Dapat disimpulkan bahwa sebagian besar guru kelas dalam pembelajaran kurang layak, sehingga diperlukan kegiatan supervisi individual. Tujuan penelitian ini adalah untuk meningkatkan kemampuan mengajar guru kelas. Penelitian ini menggunakan metode observasi kelas dan study dokumen. Sedangkan metode analisis data menggunakan data kuantitatif dan kualitatif. Lokasi penelitian di SD Negeri Kertasari 04 UPTD Dikbud Kecamatan Surdadi Kabupaten Tegal. Obyek tindakan dalam penelitian ini adalah kemampuan mengajar pada guru kelas. Penelitian ini berlangsung selama 4 bulan mulai bulan Januari sampai dengan bulan April 2018. Subjek penelitian ini adalah guru kelas I s.d VI sebanyak 6 orang. Hasil penelitian menunjukkan bahwa melalui Supervisi Individual dapat meningkatkan kemampuan guru kelas dalam kegiatan pembelajaran. Kemampuan guru dalam siklus I mencapai 50/%, pada akhir siklus II mencapai 100% terjadi peningkatan 50%. Pemahaman guru terhadap kemampuan mengajar siklus I mencapai nilai rata-rata 69,8 pada akhir siklus II mencapai nilai rata-rata 78,8 terjadi peningkatan sebesar 12,89 poin. Melalui Supervisi Individual dapat meningkatkan kemampuan guru dalam kegiatan pembelajaran.

Effect of SNEDDS (self-nanoemulsifying drug delivery system) kawista leaf aqueous extract (Limonia acidissima L.) on body and organ weight of Rats Giriani Ayu Sabilla; Slamet Widiyanto
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152113065-0-00

SNEDDS (Self-Nanoemulsifying Drug Delivery System) is a pharmacological method that can increase the bioavailability of a drug. Kawista leaf aqueous extract contains secondary metabolites in the form of flavonoids, tannins, and saponins. SNEDDS preparation of kawista leaf aqueous extract has not been reported on its effect on body weight and organs of rats. The purpose of this study was to determine the effect of SNEDDS preparation of kawista leaf aqueous extract on body weight, liver and kidney weight of Rattus norvegicus in acute toxicity testing using the OECD 423 method. Data on body weight and organ weight of the liver and kidneys of female white rats of the Wistar strain were obtained during toxicity testing by acute OECD method 423. In this test, rats were divided into 3 treatment groups,namely control, SNEDDS, and SNEDDS of kawista leaf aqueous extract, with a total of 5 rats per treatment. The test was carried out in 2 terms of dosing according to OECD 423, wherein the second term the amount of the dose given was determined from the data on the response of the test animals to the dosage of the preparations in the first term. The results were analyzed using One Way ANOVA followed with Duncan. The results showed that the SNEDDS preparation of kawista leaf aqueous extract did not affect the body weight or the weight of the liver and kidneys of rats which was indicated from the statistical test results that there was no significant difference between treatments. This study conclude that SNEDDS preparation of kawista leaf aqueous extract did not affect the body weight or liver and kidney organs of rats.SNEDDS (Self-Nano Emulsifying Drug Delivery System) merupakan metode yang dapat meningkatkan bioavailabilitas suatu obat. Ekstrak air daun kawista mengandung metabolit sekunder berupa flavonoid, tanin, dan saponin. Sediaan SNEDDS ekstrak air daun kawista belum dilaporkan pengaruhnya terhadap berat badan dan organ tubuh tikus. Tujuan penelitian ini adalah untuk mengetahui pengaruh sediaan SNEDDS ekstrak air daun kawista terhadap bobot badan, bobot hati dan bobot ginjal Rattus norvegicus pada uji toksisitas akut menggunakan metode OECD 423. Data bobot badan dan bobot organ hati dan ginjal tikus putih betina galur Wistar diperoleh selama pengujian toksisitas dengan metode OECD akut 423. Pada pengujian ini tikus dibagi menjadi 3 kelompok perlakuan yaitu kontrol, SNEDDS, dan SNEDDS ekstrak air daun kawista, dengan jumlah tikus per perlakuan sebanyak 5 ekor. Pengujian dilakukan dalam 2 dosis menurut OECD 423, dimana jumlah dosis kedua yang diberikan ditentukan dari data respon hewan uji terhadap dosis pertama. Hasil dianalisis menggunakan One Way ANOVA dilanjutkan dengan Duncan. Hasil penelitian menunjukkan bahwa sediaan SNEDDS ekstrak air daun kawista tidak berpengaruh terhadap bobot badan atau bobot hati dan ginjal tikus yang ditunjukkan dari hasil uji statistik tidak terdapat perbedaan bermakna antar perlakuan. Penelitian ini menyimpulkan bahwa sediaan SNEDDS ekstrak air daun kawista tidak berpengaruh terhadap bobot badan, hati dan ginjal tikus.

Penentuan Kualitas Telur Ayam F1 dan F2 Anakan Biak Selektif Broiler Cobb 500 x Pelung I Wayan Swarautama Mahardhika; Hendry T.S.S.G. Saragih; Slamet Widiyanto; Budi Setiadi Daryono
Buletin Veteriner Udayana Vol. 14 No. 2 April 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Egg quality can determine the fertility and reproductive efficiency of chickens. Selective breeding of Kambro chickens might have influenced the egg quality. This study aims to determine the influence of a selective breeding program on the egg quality of two Kambro chicken generations, F1 Kambro (F2K)-crossbred and F2 Kambro (F2K)-inbred. Inbreeding coefficient (F) and rate of inbreeding (Fx) of F2K chickens were calculated. The egg collection record was analyzed based on its productivity, phenotype, weight, index, and exterior egg quality parameters. Exterior egg quality parameters were analyzed with mathematical formulae. Egg productivity in Hen Day Production (HDP) of F1K and F2K chickens for 270-days, respectively, was 24% and 16.5%. The heterosis value of F2K chicken was -31.25% indicating an inbreeding depression in egg productivity. The egg phenotype of F2K was classified into light-brown, cream, brown, and white. The mean egg weight (EW) of F1K and F2K, respectively, was 53.34 ± 2.34-grams and 54.92 ± 9.25-grams, thus were classified as small eggs. The eggshape index of F1K and F2K, respectively, were dominated by sharp eggs and round eggs. Exterior egg quality parameters of F1K and F2K based on GMD could be used as a predictor variable, meanwhile Sp (r = 1, p<0.001) could not be used to describe the disposition of the regression curve. The Fx and F values of F2K, respectively, were 4.925% and 25% close to the tolerance level of inbred-line inbreeding depression. Inbreeding depression was correlated with productivity and egg characteristics depression between F1K and F2K. Parental selection in a selective breeding program must be based on several factors, including inbreeding depression, inbreeding coefficient, inbreeding rate, and egg quality. The implementation of genomic selection may provide a solution to minimalize the effect and fluctuations of Fx and F.

Fourier Transform Infrared Sebagai Metode Alternatif Penetapan Tingkat Stres pada Sapi (FOURIER TRANSFORM INFRARED AS AN ALTERNATIVE TOOL FOR DETERMINING OF STRESS IN COW) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Amelia Hana; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.145 KB)

Stress in animal is a condition of nonspesific discomfort which cause of non-specific immune defects,failure of reproduction, and decreased of meat carcass until the death of animals. To determine stress ofcattle, it will be invented stress detector using a non-invasive method based on the spectroscopy FourierTransform Infrared (FTIR). Basically, FTIR will detect component in compound of cathecolamine andcortisol as ketone (= O) and methyl (= CH 3). Furthermore, each group of components will be detected indifferent of absorbant and wavelength. The results showed that average level of cortisol in female beefcattle durimg resting eriod was 38,48±21,53 ng/dL, on time of slaughtering were 116,88±112,59 ng/dL. Forbull, which were resting 20,42±9,25 ng/dL; when animal was slaughtered level of cortisol was 67,61±41,62ng/dL. Using FTIR, it was showed that compound of metil was absorbed well. Animals with udder stresscondition have been recorded on the wave lenght of 2777-3456 nm. It has been concluded level of cortisolon cattle which were resting is significantly different from animal which were slaughtering P(< 0.05),where cortisol would increase drastically. Using Calibration of FTIR indicated resting animals only havefewer value of absorbance than animals which slaughtered. FTIR is a very prospect method for makingstress indicator.

Pemanfaatan Electronic Nose sebagai Sensor Kimiawi Urin Guna Melacak Birahi Sapi (ELECTRONIC NOSE AS URINARY CHEMICAL SENSOR FOR DETERMINING ESTROUS PHASE IN CATTLE) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Luthfiralda Sjahfirdi; Hera Maheshwari
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.873 KB)

The timing of artificial insemination relies largely on behavioral observation of estrus. The problemfaced is that not all cattle shows signs of estrus significantly, which affect the accuracy of insemination,and therefore,the success rate of Artificial Insemination is less than 50%. Recently, the determination ofestradiol levels as an indicator of estrus is done by observation of physical signs and doing ELISA test,which is expensive and provide longer time. In order to solve these problems, a tool estrus detector is madenamely Electronic Nose (EN). Determination of estrus with EN is cheaper because it does not need to usecomplexmaterials, just only use the samples. Mechanism of action of EN is using a sensor that is vaporized,while animals estrus will emit pheromones that are vaporized. Theaim of this study was to determinewhether the stage of estrus can be detected by using EN. Urine of female Ongole Crossbred which maintainedin Kuwang, district of Cangkringan, Yogyakarta, with BCS of 3 was used in this research. The sample wascollected shortly before injection of dinoprost as estrus synchronization then it repeated when cattle got estrus phase. The urinary sample of the estrus cattle was sensitive to methane, propane, butane, whereasin non-estrus cattle, besides the three of these component (methane, propane, butane), sensor was alsocaptured hydrogen sulfide. Furthermore, our electronic nose had been able to distinguish estrus phase andnon-estrus based on domain area.Thus, the Electronic Nose is very prospect used as a detector of estrus incattle. Hydrogen disulfide could possibly be used as an indicator comparison between cow estrus and nonestrus.

Pembangunan Infrastruktur Pertanian dan Sosial Dalam Rangka Mempersiapkan Selopamioro Mandiri Sejahtera Berbasis Potensi Lokal Sarmin Sarmin; Irkham Widiyono; Slamet Widiyanto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 1 (2016): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Tis program aims to develop agricultural infrastructure and social culture for Selopamioro empowering and prosperous based on local potential. Te priority of frst year programs were development of water reservoir in Kalidadap 2 and Srunggo, smart home fnishing in Srunggo 1, processed cashew as jam and shredded, promotion assistance in Bantul Expo event, branding and packaging innovation for cashew, to initiate government licensing, and develop standar operating procedure in processed cashew. All of programs as baseline to holistic development in Selopamioro based on local potential.

The Level of Community Participation in Supporting Community-Based Tourism on Mount Merapi Slope Muhamad Muhamad; Syamsul Maarif; Slamet Widiyanto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 7, No 2 (2021): June
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

This community service was carried out on the Mount Merapi slope, located around the 8-kilometer area, which falls in category III disaster-prone area. The main aim of this activity was to increase the community participation in developing their area through tourism. The method used here was Focused Group Discussion (FGD) that aimed at inviting and accommodating community participation, as well as a structured interview. The analysis employed 0-5 Likert scale containing score weight to identify the factors influencing participation forms and participation benefits. This research used the quantitative descriptive approach to describe the community level of participation. The result shows that the majority of residents (60%) participated in tourist village management, around 25% participated due to their awareness, around 12% participated without serious intention, and 3% did not participate. It can be concluded that they show high level of participation in supporting tourist village development in disaster-prone area.

Kidney Function Test of Female Wistar Rat (Rattus norvegicus Berkenhout, 1769) of Subchronic Toxicity Test of Arthrospira maxima and Chlorella vulgaris Mulyati Mulyati; Anita Yuliana; Slamet Widiyanto
Journal of Tropical Biodiversity and Biotechnology Vol 4, No 3 (2019): Desember
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.42306

Arthrospira maxima and Chlorella vulgaris are contained a high protein and antioxidant levels that can be used as functional foods or supplements to improve health. Furthermore, this material needs to be monitored for safety. The aims of this research were determined the subchronic toxicity test of microalgae A. maxima and C. vulgaris on kidney function. Parameters of this research were creatinine levels, urea levels, kidney index, and histological structure of the kidney glomerular. Besides that, the progression of body weight was observed. Five teen female Wistar rats (Rattus norvegicus Berkenhout,1769) divided into three groups randomly were one control group and two treatment groups using A. maxima and C. vulgaris in the same dose were 2500 mg/kg of body weight. Subchronic toxicity test was conducted by oral gavage every day during 90 days — the measurement of creatinine levels and urea levels on the 30th, 60th, and 90th day. Kidney index and glomerular histology of rat’s kidney was prepared after necropsy at the end of this research. Base on the results, it can be concluded that consume of Arthrospira maxima, and Chlorella vulgaris at 2500 mg/kg of body weight increased creatinine and urea levels. Bodyweight, kidney index, and pathological glomerular cells of histological kidney were still in normal value.

Effect of Arthrospira maxima and Chlorella vulgaris to Lipid Profile and Visceral Fat Index Alteration in Streptozotocin-Induced Hyperglycemia Rats Mulyati Mulyati; Aprilia Rahmawati; Slamet Widiyanto
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 1 (2020): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.49937

Arthrospira maxima and Chlorella vulgaris contain protein, carbohydrates, antioxidants, omega-3 fatty acids, and many micronutrients. Those compounds have potency of antidiabetic and hypolipidemic activity. This study aimed to evaluate the effect of A. maxima and C. vulgaris powder administration on alteration of body weight, lipid profile, glucose levels, and visceral fat index of hyperglycemia rats. Twenty male rats were divided into 5 groups i.e. negative control (NC), hyperglycemia control (HC), metformin (M), A. maxima (AR), and C. vulgaris (CH). Body weight and visceral fat index were measured and calculated by semianalytic and analytical scales. Serum glucose levels were measured by Easy Touch GCU (Glucose, Cholesterol, Uric acid). Lipid profile levels were measured using the photometric enzymatic method. The results showed no differences in body weight between groups, except in AR group was found significantly decreased in body weight on the 20th day. Glucose serum, total cholesterol, HDL and triglyceride levels in microalgae treatment groups were not significantly different be compare to control group. LDL levels of D30 significantly different from D0, but neither between groups. The visceral fat index of a control group was higher compared to that of a microalgae group and significantly different. In conclusion, the administration of microalgae A. maxima and C. vulgaris for 30 days are effective to reduce visceral fat index but not effective to maintain body weight, glucose level, as well as not effective to improve lipid profile.

Co-Authors Aeniah, Siti Amelia Hana Anita Yuliana Aprilia Rahmawati Ardaning Nuriliani ARI SUSILOWATI Ariesti, Wiwin Bambang Retnoaji BUDI SETIADI DARYONO Claude Mona Airin Dwi Liliek Kusindarta Eko Agus Suyono Empra, Desi Eka Putri Fajar Sofyantoro Giriani Ayu Sabilla Halim, Shuha Ma’muriyah Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih HERA MAHESHWARI I Wayan Swarautama Mahardhika Irkham Widiyono Laksmindra Fitria LUTHFIRALDA SJAHFIRDI Muhamad Muhammad Muhammad Mulyati Mulyati Mulyati Mulyati Mulyati Sarto Nastiti Wijayanti Prabowo Purwono Putro Pudji Astuti PUDJI ASTUTI Pusparini, Nur Ainun Oktavia Rahadian Yudo Rey, Juniman Rohmah, Zuliyati Salamah, Rohmi Sarmin Sarmin Septriani, Nur Indah Syamsul Maarif

Title

Found 16 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 16 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 16 Documents
Search