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All Journal Bioscience Biota Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati Molecular and Cellular Biomedical Sciences (MCBS) Animal Production : Indonesian Journal of Animal Production Biota Journal of Aquaculture and Fish Health Jurnal Inovasi Pembelajaran Karakter Berkala Penelitian Hayati Prosiding Konferensi Nasional PKM-CSR Jurnal Pengabdian Masyarakat Charitas Rumphius Pattimura Biological Journal Berkala Ilmiah Biologi

Slamet Widiyanto

Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 415791

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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STRESS ESTIMATION OF PRE-SLAUGHTER AND SLAUGHTERED BY MEANS OF FOURIER TRANSFORM INFRARED SPECTROSCOPY ANALYSIS THROUGH MEASUREMENT OF CORTISOL AND CATECHOLAMINE LEVEL IN FEMALE CATTLE URINE Widiyanto, Slamet; Widiyono, Irkham; Putro, Prabowo Purwono; Astuti, Pudji
ANIMAL PRODUCTION Vol 16, No 3 (2014): September
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (836.056 KB)

Abstract. This study was aimed to analyze the levels of catecholamine and cortisol as stress indicator by invasive and non-invasive methods. Twelve female cattle PO were used in this study and were collected from slaughter house in Yogyakarta. Catecholamine and cortisol level of urine were measured by Enzyme linked Immunoassay (EIA) method and Fourier Transform Infrared (FTIR), were statistically analyzed to determine the difference between pre slaughter and slaughter conditions. FTIR spectra were analyzed using chemo-metrics software. These results showed that the concentration of urinary cortisol were 2.12±1.68 ng/dl of pre-slaughter and 7.58±3.89 ng/dl of slaughtered respectively. The levels of urinary catecholamine in pre slaughter and slaughter were 3.07±2.05 ng/dl and 4.15±2.68 ng/dl respectively. In FTIR analysis showed the spectral separation between the different quadrants before and during slaughter. The result suggested the correlation between the results of the analysis using the EIA and FTIR spectra. It is assumed that the separation of the FTIR spectrum in line with the increased levels of cortisol catecholamine and samples. It can be concluded that FTIR can be used to analyze the status of stress in animals, especially in cattle. Key words: catecholamine, cortisol, non-invasive, FTIR Abstrak. Penelitian ini bertujuan untuk menganalisis kadar katekolamin dan kortisol sebagai indicator stress secara invasif maupun non invasif dan mengembangkan metode deteksi stress pada sapi. Penelitian menggunakan sampel urin sapi Peranakan Ongole (PO) yang diambil dari rumah potong hewan di Yogyakarta. Analisis kadar katekolamin (CA) dan kortisol (CO) dilakukan dengan menggunakan metode EIA dan FTIR. Data konsentrasi kortisol maupun katekolamin hasil pengukuran menggunakan metode EIA dilakukan analisis statistik untuk mengetahui pengaruh stres terhadap konsentrasi CA dan CO urine. Hasil penelitian ini menunjukan rata-rata konsentrasi kortisol urin adalah 2.12±1.68 ng/dl dalam kondisi prapenyembelihan dan 7,58±3,89 ng/dl pada saat penyembelihan. Sedangkan konsentrasi katekolamin urin pada saat prapenyembelihan dan penyembelihan secara berurutan adalah 3,07±2,05 ng/dl dan 4,15±2,68 ng/dl. Analisis FTIR menggambarkan pemisahan spektral pada quadran berbeda antara sebelum dan saat penyembelihan. Berdasarkan hasil ini menunjukkan korelasi antara hasil analisis dengan menggunakan EIA dan FTIR, baik konsentrasi katekolamin dan kortisol dengan spektra FTIR dari sampel. Perbedaan konsentrasi CA dan CO urin prapenyembelihan dan saat penyembelihan sejelan dengan pemisahan spektra FTIR. Hal ini diyakini bahwa pemisahan spektrum FTIR disebabkan adanya peningkatan kadar katekolamin dan kortisol dari sampel. Dapat disimpulkan bahwa FTIR dapat untuk menganalisis status stres pada hewan khususnya pada sapi. Kata kunci : katekolamin, kortisol, non invasif, FTIR

PENINGKATAN KETERAMPILAN MENGAJAR MELALUI TEKNIK SUPERVISI INDIVIDUAL JENIS OBSERVASI KELAS PADA GURU Widiyanto, Slamet
Jurnal Inovasi Pembelajaran Karakter Vol 4, No 2 (2019): Edisi Desember 2019
Publisher : Jurnal Inovasi Pembelajaran Karakter

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Penelitian ini dilatarbelakangi oleh kompetensi kemampuan guru kelas di SD Negeri Kertasari 04 dalam mengajar masih rendah. Dapat disimpulkan bahwa sebagian besar guru kelas dalam pembelajaran kurang layak, sehingga diperlukan kegiatan supervisi individual. Tujuan penelitian ini adalah untuk meningkatkan kemampuan mengajar guru kelas. Penelitian ini menggunakan metode observasi kelas dan study dokumen. Sedangkan metode analisis data menggunakan data kuantitatif dan kualitatif. Lokasi penelitian di SD Negeri Kertasari 04 UPTD Dikbud Kecamatan Surdadi Kabupaten Tegal. Obyek tindakan dalam penelitian ini adalah kemampuan mengajar pada guru kelas. Penelitian ini berlangsung selama 4 bulan mulai bulan Januari sampai dengan bulan April 2018. Subjek penelitian ini adalah guru kelas I s.d VI sebanyak 6 orang. Hasil penelitian menunjukkan bahwa melalui Supervisi Individual dapat meningkatkan kemampuan guru kelas dalam kegiatan pembelajaran. Kemampuan guru dalam siklus I mencapai 50/%, pada akhir siklus II mencapai 100% terjadi peningkatan 50%. Pemahaman guru terhadap kemampuan mengajar siklus I mencapai nilai rata-rata 69,8 pada akhir siklus II mencapai nilai rata-rata 78,8 terjadi peningkatan sebesar 12,89 poin. Melalui Supervisi Individual dapat meningkatkan kemampuan guru dalam kegiatan pembelajaran.

Effect of SNEDDS (self-nanoemulsifying drug delivery system) kawista leaf aqueous extract (Limonia acidissima L.) on body and organ weight of Rats Giriani Ayu Sabilla; Slamet Widiyanto
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152113065-0-00

SNEDDS (Self-Nanoemulsifying Drug Delivery System) is a pharmacological method that can increase the bioavailability of a drug. Kawista leaf aqueous extract contains secondary metabolites in the form of flavonoids, tannins, and saponins. SNEDDS preparation of kawista leaf aqueous extract has not been reported on its effect on body weight and organs of rats. The purpose of this study was to determine the effect of SNEDDS preparation of kawista leaf aqueous extract on body weight, liver and kidney weight of Rattus norvegicus in acute toxicity testing using the OECD 423 method. Data on body weight and organ weight of the liver and kidneys of female white rats of the Wistar strain were obtained during toxicity testing by acute OECD method 423. In this test, rats were divided into 3 treatment groups,namely control, SNEDDS, and SNEDDS of kawista leaf aqueous extract, with a total of 5 rats per treatment. The test was carried out in 2 terms of dosing according to OECD 423, wherein the second term the amount of the dose given was determined from the data on the response of the test animals to the dosage of the preparations in the first term. The results were analyzed using One Way ANOVA followed with Duncan. The results showed that the SNEDDS preparation of kawista leaf aqueous extract did not affect the body weight or the weight of the liver and kidneys of rats which was indicated from the statistical test results that there was no significant difference between treatments. This study conclude that SNEDDS preparation of kawista leaf aqueous extract did not affect the body weight or liver and kidney organs of rats.SNEDDS (Self-Nano Emulsifying Drug Delivery System) merupakan metode yang dapat meningkatkan bioavailabilitas suatu obat. Ekstrak air daun kawista mengandung metabolit sekunder berupa flavonoid, tanin, dan saponin. Sediaan SNEDDS ekstrak air daun kawista belum dilaporkan pengaruhnya terhadap berat badan dan organ tubuh tikus. Tujuan penelitian ini adalah untuk mengetahui pengaruh sediaan SNEDDS ekstrak air daun kawista terhadap bobot badan, bobot hati dan bobot ginjal Rattus norvegicus pada uji toksisitas akut menggunakan metode OECD 423. Data bobot badan dan bobot organ hati dan ginjal tikus putih betina galur Wistar diperoleh selama pengujian toksisitas dengan metode OECD akut 423. Pada pengujian ini tikus dibagi menjadi 3 kelompok perlakuan yaitu kontrol, SNEDDS, dan SNEDDS ekstrak air daun kawista, dengan jumlah tikus per perlakuan sebanyak 5 ekor. Pengujian dilakukan dalam 2 dosis menurut OECD 423, dimana jumlah dosis kedua yang diberikan ditentukan dari data respon hewan uji terhadap dosis pertama. Hasil dianalisis menggunakan One Way ANOVA dilanjutkan dengan Duncan. Hasil penelitian menunjukkan bahwa sediaan SNEDDS ekstrak air daun kawista tidak berpengaruh terhadap bobot badan atau bobot hati dan ginjal tikus yang ditunjukkan dari hasil uji statistik tidak terdapat perbedaan bermakna antar perlakuan. Penelitian ini menyimpulkan bahwa sediaan SNEDDS ekstrak air daun kawista tidak berpengaruh terhadap bobot badan, hati dan ginjal tikus.

Pembangunan Infrastruktur Pertanian dan Sosial Dalam Rangka Mempersiapkan Selopamioro Mandiri Sejahtera Berbasis Potensi Lokal Sarmin Sarmin; Irkham Widiyono; Slamet Widiyanto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 1 (2016): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Tis program aims to develop agricultural infrastructure and social culture for Selopamioro empowering and prosperous based on local potential. Te priority of frst year programs were development of water reservoir in Kalidadap 2 and Srunggo, smart home fnishing in Srunggo 1, processed cashew as jam and shredded, promotion assistance in Bantul Expo event, branding and packaging innovation for cashew, to initiate government licensing, and develop standar operating procedure in processed cashew. All of programs as baseline to holistic development in Selopamioro based on local potential.

The Level of Community Participation in Supporting Community-Based Tourism on Mount Merapi Slope Muhamad Muhamad; Syamsul Maarif; Slamet Widiyanto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 7, No 2 (2021): June
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

This community service was carried out on the Mount Merapi slope, located around the 8-kilometer area, which falls in category III disaster-prone area. The main aim of this activity was to increase the community participation in developing their area through tourism. The method used here was Focused Group Discussion (FGD) that aimed at inviting and accommodating community participation, as well as a structured interview. The analysis employed 0-5 Likert scale containing score weight to identify the factors influencing participation forms and participation benefits. This research used the quantitative descriptive approach to describe the community level of participation. The result shows that the majority of residents (60%) participated in tourist village management, around 25% participated due to their awareness, around 12% participated without serious intention, and 3% did not participate. It can be concluded that they show high level of participation in supporting tourist village development in disaster-prone area.

Kidney Function Test of Female Wistar Rat (Rattus norvegicus Berkenhout, 1769) of Subchronic Toxicity Test of Arthrospira maxima and Chlorella vulgaris Mulyati Mulyati; Anita Yuliana; Slamet Widiyanto
Journal of Tropical Biodiversity and Biotechnology Vol 4, No 3 (2019): Desember
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.42306

Arthrospira maxima and Chlorella vulgaris are contained a high protein and antioxidant levels that can be used as functional foods or supplements to improve health. Furthermore, this material needs to be monitored for safety. The aims of this research were determined the subchronic toxicity test of microalgae A. maxima and C. vulgaris on kidney function. Parameters of this research were creatinine levels, urea levels, kidney index, and histological structure of the kidney glomerular. Besides that, the progression of body weight was observed. Five teen female Wistar rats (Rattus norvegicus Berkenhout,1769) divided into three groups randomly were one control group and two treatment groups using A. maxima and C. vulgaris in the same dose were 2500 mg/kg of body weight. Subchronic toxicity test was conducted by oral gavage every day during 90 days — the measurement of creatinine levels and urea levels on the 30th, 60th, and 90th day. Kidney index and glomerular histology of rat’s kidney was prepared after necropsy at the end of this research. Base on the results, it can be concluded that consume of Arthrospira maxima, and Chlorella vulgaris at 2500 mg/kg of body weight increased creatinine and urea levels. Bodyweight, kidney index, and pathological glomerular cells of histological kidney were still in normal value.

Effect of Arthrospira maxima and Chlorella vulgaris to Lipid Profile and Visceral Fat Index Alteration in Streptozotocin-Induced Hyperglycemia Rats Mulyati Mulyati; Aprilia Rahmawati; Slamet Widiyanto
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 1 (2020): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.49937

Arthrospira maxima and Chlorella vulgaris contain protein, carbohydrates, antioxidants, omega-3 fatty acids, and many micronutrients. Those compounds have potency of antidiabetic and hypolipidemic activity. This study aimed to evaluate the effect of A. maxima and C. vulgaris powder administration on alteration of body weight, lipid profile, glucose levels, and visceral fat index of hyperglycemia rats. Twenty male rats were divided into 5 groups i.e. negative control (NC), hyperglycemia control (HC), metformin (M), A. maxima (AR), and C. vulgaris (CH). Body weight and visceral fat index were measured and calculated by semianalytic and analytical scales. Serum glucose levels were measured by Easy Touch GCU (Glucose, Cholesterol, Uric acid). Lipid profile levels were measured using the photometric enzymatic method. The results showed no differences in body weight between groups, except in AR group was found significantly decreased in body weight on the 20th day. Glucose serum, total cholesterol, HDL and triglyceride levels in microalgae treatment groups were not significantly different be compare to control group. LDL levels of D30 significantly different from D0, but neither between groups. The visceral fat index of a control group was higher compared to that of a microalgae group and significantly different. In conclusion, the administration of microalgae A. maxima and C. vulgaris for 30 days are effective to reduce visceral fat index but not effective to maintain body weight, glucose level, as well as not effective to improve lipid profile.

Biochemical compounds and sub-chronic toxicity test of Chlorella sp. and Spirulina sp. isolated from Glagah Coastal Water Slamet Widiyanto; Mulyati Sarto; Laksmindra Fitria; Rahadian Yudo; Eko Agus Suyono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Microalgae are microscopic photosynthetic organisms found in marine and freshwater environments. This organism is a eukaryotic group of polyphyletic and very diverse. This study investigated the biochemical composition and sub-chronic test of Chlorella Sp. and Spirulina Sp. isolated from Glagah coastal water (Yogyakarta, Indonesia) as a preclinical study to create potential nutritional supplement for human health. Microalgae culture was analyzed for biochemical composition using several methods: AAS for metal analysis (Pb, Cu, Fe, Mg, Zn, and Hg), GC-MS and UV spectrophotometer for fatty acid profiles analysis (lenolenic acid, eicosatrienoic acid, arachidonic acid, linoleic acid, oleic acid, eicosenoic acid, EPA, DHA, omega 6 and 9), Alkaloids, Steroids, Tanin and Saponin. The next step is to do a sub-chronic test of microalgae biomass using experimental animals. The result shows that Spirulina sp. and Chlorella sp. contain 16 and 10 different biochemical compounds respectively. Furthermore, organic nutritional properties in both of microalgae are relatively high with a rich biochemical profile of Fe and nutritious poly-unsaturated fats (such as linolenic acid, arachidonic acid , omega 6, and 9). Regarding sub-chronic toxicity test, it was clear that Spirulina and Chlorella did not affect the blood profile. These findings suggest that both of Glagah strain microalgae are considered to have great potential as a multi-nutritional human health supplement.

Aseet Sosial Pengembangan Pariwisata Pedesaan Tangguh Bencana Berkelanjutan Muhammad Muhammad; Slamet Widiyanto
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 3 (2020): Peran Perguruan Tinggi dan Dunia Usaha Dalam Pemberdayaan Masyarakat Untuk Menyongsong
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Kegiatan Pengabdian Kepada Masyarakat (PKM) yang berlokasi di Desa Kalibening, Kecamatan Dukun, Kabupaten Magelang yang dilaksanakan sejak tahun 2013-2018 telah banyak pengetahuan yang didapatkan dari proses belajar bersama masyarakat yang ditinjau dari berbagai bidang keilmuan. Fakta yang melatarbelakangi pemilihan lokasi kelompok sasaran merupakan kawasan rawan bencana erupsi Gunung Merapi, tingkat pendidikan yang masih rendah, kemiskinan, masyarakat tidak berdaya dan lain-lain. Tujuan utama dalam kajian pelaksanaan Pengabdian Kepada Masyarakat adalah sebagai upaya membangun kesepahaman terhadap bentuk pembangunan melalui pemberdayaan masyarakat yang diharapkan dapat meningkatkan kapasitas masyarakat (capacity building) sehingga mampu memenuhi harapan dan kebutuhan masyarakat. Asset social (social mapping) pada kelompok sasaran dapat mengetahui rendahnya tingkat pengetahuan, kemiskinan dan keterampilan yang dimiliki masyarakat dan kelompok sasaran mengakibatkan mereka tidak mampu mengenali apa yang dibutuhkan dalam meningkatkan kapasitasnya beserta potensi besar yang dimilikinya. Metode dan analisis yang dilakukan dalam kajian ini lebih terfokus pada Focus Group Disscussion (FGD), wawancara mendalam, quisioner dalam upaya menjaring dan menemukenali tingkat partisipatif masyarakat dan wawancara secara terstruktur. Penelitian menggunakan rentang waktu (masa pelaksanaa) pada periode tahun 2014-2018 melalui skema Pengembangan Desa Binaan (Desbin), skema yang dikaji berdasarkan urutan waktu secara berurutan. Hasil penelitian menunjukan tingkat partisipatif yang tinggi dan mengetahui modal sosial berupa: modal ekonomi, modal alam, modal fisik, modal sumber daya manusia modal pengetahuan dan berkontribusi terhadap pengembangan potensi desa berupa pengembangan pariwisata berbasis masyarakat tangguh bencana yang berkelanjutan. Kesimpulan dan hasil perhitungan modal sosial yang didapatkan dengan nilai tertinggi sebesar modal sumber daya alam sebesar (3,9), modal sosial (3,0), modal finansial (3,2), modal fisik merupakan modal yang terendah dengan nilai (2,6).

Maturation of Female Yellow Rasbora (Rasbora Lateristriata Bleeker, 1854) Using Oodev at Different Doses in Feed Rey, Juniman; Widiyanto, Slamet; Retnoaji, Bambang
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.75916

The current high demand for Yellow rasbora (Rasbora lateristriata) is not supported by the availability of captured Yellow rasbora in nature. Aquaculture is the most rational way of utilizing biological natural resources. In intensive aquaculture, it is necessary to optimize all processes that occur in aquaculture, including hatchery. However, the common problem that often happens in hatchery activities is spawning which depends on the season. The hormonal manipulation technique is an appropriate way to stimulate gonadal maturation. Oodev is a hormonal combination of pregnant mare serum gonadotropin and anti-dopamine to stimulate gonadal maturation. The purpose of this study was to determine the effectiveness of using the Oodev with different doses in feed to accelerate gonad maturation of female Yellow rasbora. The study was carried out with four treatments and three replications in 21 days with different doses of Oodev, such as; A (Feed without Oodev), B (0.5 mL/kg feed), C (1 mL/kg feed) and D (2 mL/kg feed). The parameters observed in this study were gonad maturity level, histological structure of ovary, gonadosomatic index, fecundity, and diameter of eggs. The results showed that the dose of Oodev at 1.0 mL/kg feed was an effective dose to optimize the gonad maturity of female Yellow rasbora. This is proven by the highest results shown on all parameters, such as; the maturity level in the IV phase, histological structure of the ovary which showed the dominance of the oocyte maturation phase, gonadosomatic index of 14.014%, the fecundity of 721 eggs, and egg diameter of 0.865 mm. In conclusion, using Oodev in feed at a dose of 1.0 ml/kg of feed for 21 days is an effective dose to optimize the maturation of female Yellow rasbora.

Co-Authors Abdul Kholil Adelfiani, Adelfiani Aeniah, Siti Amelia Hana Anita Yuliana Aprilia Rahmawati Ardaning Nuriliani ARI SUSILOWATI Ariesti, Wiwin Bambang Retnoaji BUDI SETIADI DARYONO Claude Mona Airin Dafa, Maula Haqul Dwi Liliek Kusindarta Eko Agus Suyono Empra, Desi Eka Putri Fajar Sofyantoro Giriani Ayu Sabilla Halim, Shuha Ma’muriyah Handayani, Lisa Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih HERA MAHESHWARI Hurul Ein I Wayan Swarautama Mahardhika Illiyin, Sirayya Irkham Widiyono Kartika Wulandari Khoirul Huda Laksmindra Fitria LUTHFIRALDA SJAHFIRDI Mayani, Olvita Muhamad Muhammad Muhammad Mulyati Mulyati Mulyati Mulyati Mulyati Sarto Nastiti Wijayanti Oktaviana, Shintia Pertiwi, Anggita Endar Podhi, Felisitas Moli Prabowo Purwono Putro Primahesa, Alfian Prissandi, Anthera Al Firdaus Pudji Astuti PUDJI ASTUTI Pusparini, Nur Ainun Oktavia Rahadian Yudo Rahayu, Sekar Rey, Juniman Rohmah, Zuliyati Rumansara, Papuani Samparisna Sadewo, Imran Salamah, Rohmi Saragih, Hendry T. S. S. G. Sari, Annisa Lintang Sarmin Sarmin Septriani, Nur Indah Septriani, Nur Indah Simanungkalit, Eben Ezer Siswara, Hamzah Nata Sofyantoro, Fajar Sularsah, Sais Syamsul Maarif

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