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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 357 Documents
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Preface Squalen Bulletin Vol. 12 No. 1 Tahun 2017 Squalen Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.312

Abstract

Effect of Agitation Speed and Cultivation Time on the Production of the Emestrin Produced by Emericella nidulans Marine Fungal muhammad nursid nursid; Martua Manulang; Joko Samiadji; Endar Marraskuranto
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 2 (2015): August 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i2.122

Abstract

Emestrin, an epipolythidioxopiperazine (EPT), is bioactive secondary metabolite produced by the marine fungus Emericella nidulans. Emestrin is potential to be developed as anticancer agent. Our present study investigated the effect of the agitation speed and cultivation time on the production of the mycelial biomass and emestrin in E. nidulans. The fungal was cultivated in malt extract broth (MEB) medium with varying agitation speeds of 0, 50, 100,150 rpm during 1,2,3 and 4 weeks of incubation at the temperature of 28 oC. Concentration of emestrin was determined by using high performed liquid chromatography (HPLC). The highest concentration of emestrin was found at static condition (0 rpm) cultivated for 1 week.
Identification and Antimicrobial Activity of Marine Streptomyces from Geographically Different Regions of Indonesia Rofiq Sunaryanto; Anis Herliyani Mahsunah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i1.101

Abstract

Identification and antimicrobial assay of indegenous marine Streptomyces have been conducted. Samples were obtained from culture collection of Biotech Center, Agency for the Assessment and Application of Technology (BPPT). They were originated from several Indonesian seashores including Pelabuhan Ratu, Pangandaran, Manokwari, Pulau Seribu, Garut, Bangka, Banjarmasin, Belitung, Cirebon, and Palu. Isolates stored as glycerol stocks were inoculated onto HV (Humic acid vitamin) agar and incubated for 5 days at 30 °C. Each colony was cultivated using yeast-peptone medium and then extracted by butanol. Antimicrobial activities were monitored by the agar diffusion paper-disc method against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 66923, Pseudomonas aeruginosa ATCC 27853, Candida albicans BIOMCC 00122 and Aspergillus niger BIOMCC 0013. Molecular identification of Streptomyces was carried out based on 16S rRNA gene analysis. Our research results showed that 71 isolates obtained from several Indonesian seashore were identified as 57 different Streptomyces species. Fifty of them showed antimicrobial activity. Twenty three isolates inhibited B. subtilis ATCC 66923, 14 isolates inhibited S. aureus ATCC 25923, 24 isolates inhibited C. albicans BIOMCC 00122 and 26 isolates inhibited A. niger BIOMCC 0013 and there was no active isolates inhibited the growth of E. coli ATCC 25922 and P. aeruginosa ATCC 27853. Phylogenetic analysis showed that Streptomyces isolates originated from the same geographically region was not necessarily grouped into the same cluster. Likewise a phylogenetic cluster may contain isolates of the same Streptomyces species, but from geographically different locations.
Back Cover Squalen Bulletin Vol. 12 No. 3 Tahun 2017 Squalen Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 3 (2017): December 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i3.317

Abstract

Teknologi Penanganan dan Transportasi Lobster Air Tawar Theresia Dwi Suryaningrum; Syamdidi Syamdidi; Diah Ikasari
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 2, No 2 (2007): December 2007
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v2i2.135

Abstract

Lobster air tawar yang diperdagangkan dalam keadaan hidup, memerlukan serangkaian kegiatan yang meliputi penanganan pascapanen, penampungan dan pengemasan sebelum  dilakukan transportasi.  Penanganan pascapanen ditujukan untuk sortasi, dengan memisahkan lobster berdasarkan ukuran dan kebugarannya.  Penampungan dilakukan bila lobster yang akan dipasarkan jumlahnya tidak mencukupi atau lobster perlu dibugarkan terlebih dahulu sebelum dikirim. Penampungan dapat dilakukan dengan menggunakan rak bertingkat yang dilengkapi dengan sistem filtrasi dengan sistem aliran tertutup.  Lobster sebelum dikirim dilakukan imotilisasi dengan suhu dingin (12oC), kemudian dikemas dalam plastik mika yang pada bagian dasarnya diberi spon basah. Plastik mika kemudian dimasukkan ke dalam kotak styrofoam yang pada bagian dasar dan atasnya diberi es yang dibungkus dalam plastik.  Kotak styrofoam kemudian direkatkan dengan lakban dan dimasukkan ke dalam karton untuk dikirim. Dengan kemasan seperti ini transportasi lobster air tawar dapat dilakukan sampai dengan 6 hari dengan sintasan 97%. Ketahanan lobster yang cukup lama di luar media memungkinkan lobster dapat diekspor dalam keadaan hidup ke berbagai negara dimana peluang pasar lobster air tawar masih sangat terbuka. 
Back Cover Squalen Bulletin Vol. 11 No. 2 Tahun 2016 Squalen Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.325

Abstract

Teknik Pemekatan, Purifikasi dan Karakterisasi Protein Rekombinan Dedi Noviendri; Sugiyono SG
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 1, No 1 (2006): December 2006
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v1i1.74

Abstract

Teknik Pemekatan, Purifikasi dan Karakterisasi Protein Rekombinan
Bioassay-Guided Isolation of an Antibacterial Compound from the Indonesian Soft Coral Masteria Yunovilsa Putra; Ahmad Saparhadi; Firmansyah Karim; Tutik Murniasih; Respati Tri Swasono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.246

Abstract

With the aim of searching for new antibacterial compound from marine soft corals, the investigation had been conducted on antibacterial activity of an extract from soft corals Sarcophyton trocheliophorum in the n-hexane, ethyl acetate, n-butanol, and aqueous fractions. The antibacterial activity was tested against two Gram-positive bacteria, viz. Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923), and two Gram-negative bacteria, viz. Escherichia coli (ATCC 25922) and Vibrio cholerae (ATCC 14035) using the agar disc diffusion assay. Among them, the n-hexane fraction was the most active against three tested bacteria, viz. Bacillus subtilis, Staphylococcus aureus and Vibrio cholerae at the concentration 125 µg/ml, with inhibition zone 14.2, 18.2, 13.8 mm, respectively. Isolation and purification of the active component from the n-hexane fraction led to a known cembranoid-type diterpene, sarcophytoxide. The chemical structure of the isolated compound was determined by IR, MS and NMR, as well as compared to data from the literature. Sarcophytoxide showed moderate activity against B. subtilis, S. aureus and V. cholerae, with a minimum inhibitory concentration (MIC) of 125, 100, 125 mg/ml, respectively.
MICROBIOLOGICAL RISK ASSESSMENT OF FISHERY PRODUCT IN INDONESIA: A PROPOSED MODEL FOR THE RISK OF Vibrio parahaemolyticus IN SHRIMP Novalia Rachmawati; Radestya Triwibowo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 2 (2012): August 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i2.20

Abstract

Increasing fish consumption value should be supported by enhancing the safety and quality offish products. Microbiological level is most of importance since every food contains microorganismswhich could multiplicate due to temperature abuse and time delay during handling and processing.Risk assessment is a structurally and scientifically based approach aimed to protect consumerfrom risk (hazard) particularly microbiological hazard when consuming certain food. Microbiologicalrisk assessment of fishery products have not been structurally developed in Indonesia, eventhoughseveral initial data on hazard identification have been available. As an attempt to build an integratedrisk assessment, a model for microbiological risk of pathogenic  Vibrio parahaemolyticus inshrimp will be proposed in this article. The available data could be used as a starting point whileother required data could be collected in collaboration with other related institutions.
TEKNIK ZYMOGRAM DAN APLIKASINYA DALAM RISET ENZIM Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 1 (2009): May 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i1.151

Abstract

Teknik zymogram merupakan pengembangan dari teknik elektroforesis yang di dalam gel elektroforesis disisipkan substrat sehingga protein/enzim yang telah dipisahkan sekaligus dapat dipelajari kemampuannya dalam mendegradasi substrat tersebut. Teknik elektroforesis sendiri merupakan teknik yang biasa digunakan dalam bidang biokimia untuk memisahkan campuran yang berisi biomolekul seperti enzim sehingga enzim yang ada akan terpisah berdasarkan muatan dan berat molekulnya. Teknik zymogram telah luas digunakan, diantaranya  untuk deteksi jenis-jenis enzim yang disekresikan oleh mikroba dan mempelajari karakteristiknya. Dengan semakin berkembangannya peran protein/enzim dalam berbagai bidang kesehatan, teknik zymogram semakin populer dan diperluas aplikasinya.

Page 6 of 36 | Total Record : 357

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