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All Journal PROSIDING SEMINAR NASIONAL JURNAL KIMIA SAINS DAN APLIKASI Majalah Obat Tradisional Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Indonesian Journal of Chemistry Jurnal Biomedika Journal of Multidisciplinary Applied Natural Science

Respati Tri Swasono

Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281

Author-ID : 511339

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Articles

Konversi Limbah Cangkang Kepiting Menjadi Chitosan Beads sebagai Matriks Pendukung pada Proses Imobilisasi Lipase Susan Primadevi; Tri Joko Raharjo; Respati Tri Swasono
Biomedika Vol 7 No 2 (2014): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Limbah cangkang kepiting dapat diproses menjadi kitosan melalui reaksi deproteinasi, demineralisasi dan deasetilasi. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan chitosan beads sebagai matriks pendukung pada proses imobilisasi lipase yang mampu meningkatkan stabilitas termal enzim lipase bebas. Kitosan yang diperoleh dianalisis dengan FTIR dan dikarakterisasi kadar air, kadar abu dan kadar nitrogen. Tahap selanjutnya, kitosan dimodifikasi bentuknya menjadi chitosan beads melalui proses penggembungan kemudian diikat silang dengan glutaraldehid. Chitosan beads yang sudah diikat silang diimobilisasikan ke dalam larutan lipase 1% yang dilarutkan dalam bufer fosfat pH 6. Enzim lipase terimobilisasi diuji stabilitas termalnya melalui reaksi transesterifikasi antara minyak kelapa dengan metanol serta diamati aktivitas transesterase enzim lipase bebas dan enzim lipase terimobilisasi. Hasil penelitian menunjukkan bahwa kitosan memiliki derajat deasetilasi 70,53% berdasarkan baseline-a dan 82,84% berdasarkan baseline-b. Kadar air, kadar abu dan kadar nitrogen kitosan berturut-turut yaitu 14,13Â±0,01%, 0,75Â±0, 13% dan 7,27Â±0,09%. Stabilitas termal enzim lipase terimobilisasi mengalami penurunan sebesar 20-40%, sedangkan enzim lipase bebas mengalami penurunan sebesar 30-45% pada temperatur pemanasan awal 35-45 Â°C. Penurunan aktivitas terbesar terjadi pada saat temperatur pemanasan awal 45-50 oC yaitu 51,81% untuk enzim lipase terimobilisasi dan 97,29% untuk enzim lipase bebas.

UJI STABILITAS ENZIM LIPASE TERIMOBILISASI PADA KITOSAN SERBUK MELALUI TEKNIK TAUT SILANG Fandhi Adi Wardoyo; Tri Joko Raharjo; Respati Tri Swasono
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.718 KB)

Lipase has a great catalytic power, but easily influenced by environments and difficult to separate at the ends of the reaction, so it can not be re-used. Based on that, one solution to overcome that problem is through the enzyme immobilization. Immobilization of lipase on chitosan powder was performed via cross linking technique. Chitosan was prepared from crab shell with deacetylation degree 82,34% and crosslinked with glutaraldehide. The aims of this research is to determine the thermal stability and multiple-use stability through the hydrolysis reaction of palm oil. Immobilized lipase on chitosan powder was able to maintain its activity at 50 oC, and still can produce free fatty acid as much as 48,55%. The immobilized lipase was also able to maintain its activity after multiple-uses up to five reaction cycles, and still can produce free fatty acid as much as 40,82%.Keywords: lipase, immobilization, chitosan, cross linking

1H NMR Fingerprinting of Medicinal Herbs Contain Chemical Drug Material Allopurinol Adita Yuniati Puspitasari; Harno Dwi Pranowo; Respati Tri Swasono; Tri Rini Nuringtyas
Majalah Obat Tradisional Vol 23, No 3 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

A study to differentiate the pure medicinal herbs from the mix medicinal herbs with chemical drug material has been done. For this purpose, we conducted fingerprinting of commercial medicinal herbs and chemical drug material allopurinol using 1H-NMR followed with chemometrics analysis. Nine commercial traditional herbal medicines claimed for rheumatic were used as samples as well as allopurinol as the chemical drug standard. Extraction of samples was done by ultrasonicator for 15 min in methanol-d4 containing 0.01% TMSP as an internal standard. Each type of herbal medicine was prepared in three replicates. The phytochemical analysis was done by 500 MHz JEOL NMR. The chemometrics analysis was done using SIMCA software following the 1H NMR spectra processing with MNOVA software. All spectra showed no contamination with allopurinol. The specific signals of allopurinol at aromatic regions were confirmed not present when the spectra were stacked together. Hence, the result of OPLS-DA analysis convinced that the herbs were clearly separated the medicinal herbs into 3 classes. Jamu 1 is separated from others showed very high intensity of several signals which may indicate an addition of chemical medicines but not allopurinol. The clear separation of other two groups may corresponds to the similarity of ingredients. These results also showed that most of traditional medicines which produced by small industries, the traditional medicines contain no active pharmaceutical ingredients (allopurinol) indicating a high safety of Indonesia traditional medicines.

Bioassay-Guided Isolation of an Antibacterial Compound from the Indonesian Soft Coral Masteria Yunovilsa Putra; Ahmad Saparhadi; Firmansyah Karim; Tutik Murniasih; Respati Tri Swasono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 2 (2016): August 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i2.246

With the aim of searching for new antibacterial compound from marine soft corals, the investigation had been conducted on antibacterial activity of an extract from soft corals Sarcophyton trocheliophorum in the n-hexane, ethyl acetate, n-butanol, and aqueous fractions. The antibacterial activity was tested against two Gram-positive bacteria, viz. Bacillus subtilis (ATCC 6633) and Staphylococcus aureus (ATCC 25923), and two Gram-negative bacteria, viz. Escherichia coli (ATCC 25922) and Vibrio cholerae (ATCC 14035) using the agar disc diffusion assay. Among them, the n-hexane fraction was the most active against three tested bacteria, viz. Bacillus subtilis, Staphylococcus aureus and Vibrio cholerae at the concentration 125 µg/ml, with inhibition zone 14.2, 18.2, 13.8 mm, respectively. Isolation and purification of the active component from the n-hexane fraction led to a known cembranoid-type diterpene, sarcophytoxide. The chemical structure of the isolated compound was determined by IR, MS and NMR, as well as compared to data from the literature. Sarcophytoxide showed moderate activity against B. subtilis, S. aureus and V. cholerae, with a minimum inhibitory concentration (MIC) of 125, 100, 125 mg/ml, respectively.

Characterization and Photocatalytic Activity of TiO2(rod)-SiO2-Polyaniline Nanocomposite Sri Wahyuni; Eko Sri Kunarti; Respati Tri Swasono; Indriana Kartini
Indonesian Journal of Chemistry Vol 18, No 2 (2018)
Publisher : Universitas Gadjah Mada

A study of TiO2(rod)-SiO2 composites coated with polyaniline (PANI) has been performed. PANI was synthesized through in-situ polymerization of aniline at various concentration (0.0137, 0.0274, and 0.0411 M) on the composite under acidic condition. PANI was confirmed by the appearance of C=N, C=C vibrations and the redshift of the band-gap from 3.14 eV for the TiO2(rod)-SiO2 into 3.0 eV for the TSP01 composite. It is also shown that the polymerization does not change the crystal structure of TiO2(rod)-SiO2 as confirmed by the XRD pattern. The TEM image shows a mixed structure of SiO2 coated by TiO2(rod)-PANI layers and the oxides coated by PANI layers. Therefore, the surface area of the resulted TiO2(rod) and the composites did not change significantly. The T TiO2(rod)-SiO2-PANI composite give small improvement under visible irradiation from 20.25 to 25.59% (around 5% from the bulk of TiO2(rod)) and from 25.03 to 25.59% (around 2% from TiO2(rod)-SiO2 composite). The mixed structure of the composites, as well as the formation of excessive layers of PANI, are possibly the case for the low photoactivity. Further improvement to obtain a core-shell structure with a thin layer of PANI is still sought.

Short Time Synthesis of Titania Nanotubes: Effect of Pre-Mixing Prior Hydrothermal Indriana Kartini; Ira Nur Arba’atul Jannah; Fitri Rizki Amalia; Salim Mustofa; Eko Sri Kunarti; Respati Tri Swasono
Indonesian Journal of Chemistry Vol 19, No 1 (2019)
Publisher : Universitas Gadjah Mada

The effect of pre-mixing by mechanical stirring before hydrothermal and hydrothermal time on the crystalline phases and morphology of titania has been studied. It was shown that nanotubes titania can be obtained after 5 h hydrothermal at 150 °C. The XRD patterns and Raman spectra of the produced powders showed the existence of anatase and titanate crystalline phases. At the longest stirring, TiO2 (B) was observed. High textural coefficient for [200] plane of anatase (TC200) confirmed oriented growth of one-dimensional anatase along [200]. All powders resulted at various stirring time were nanotubes, as confirmed by Transmission Electron Microscope (TEM). It was found that the longer the stirring, the higher the surface area of the nanotubes. All powders showed type-IV isotherm for nitrogen gas adsorption/desorption, indicating the existence of mesoporous materials. However, long hydrothermal induced the nanospheres formation, hence reducing the surface area. The band-gap of the resulted titania nanotubes were ranging from 3.11–3.16 eV. The photocatalytic performance toward the degradation of methylene blue of the titania nanotubes was higher (~50%) compared to the bulk TiO2 (~5%) under visible-light and was comparable under UV-light (~60%). These results pave a way of producing visible-sensitive TiO2 photocatalyst by altering the morphology.

Identification α-Amylase Inhibitors of Vernonia amygdalina Leaves Extract Using Metabolite Profiling Combined with Molecular Docking Norainny Yunitasari; Tri Joko Raharjo; Respati Tri Swasono; Harno Dwi Pranowo
Indonesian Journal of Chemistry Vol 22, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.71499

Vernonia amygdalina was reported to be used as a therapy for Diabetes Mellitus (DM). One of the mechanisms of therapy DM was to inhibit the action of the α-amylase enzyme. This study aimed to prove the presence of compounds that could inhibit the action of α-amylase. Vernonia amygdalina leaves were macerated with methanol and partitioned into n-hexane, dichloromethane (DCM), and ethyl acetate (EtOAc). Furthermore, they were tested for α-amylase inhibitory activity and analyzed using liquid chromatography-high resolutions mass spectrometry (LC-HRMS). Molecular docking and molecular dynamics simulation (MD simulation) examined unique compounds in the extract with good activity and chromatogram results. The EtOAc extracts showed potential as α-amylase inhibitors indicated by their IC50 values, namely 3.0 μg/mL. There are five unique compounds in the EtOAc extract predicted as 3-[(2Z)-3,7-dimethylocta-2,6-dien-1-yl]-2,4-dihydroxy-6-(2-phenylethyl)benzoic acid (compound 1), 2-hexylpentanedioic acid (compound 2), (2E,4E)-5-[1-hydroxy-2,6-dimethyl-4-oxo-6-({3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl] oxy}methyl)cyclohex-2-en-1-yl]-3-methylpenta-2,4-dienoic acid (compound 3), 3,5,5-trimethyl-4-(3-{[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-1-yl)oxy}butyl)cyclohex-2-en-1-one (compound 4), and 2-{[(6E)-2,10-dihydroxy-2,6,10-trimethyldodeca-6,11-dien-3-yl]oxy}-6-(hydroxymethyl)oxane-3, 4,5-triol (compound 5). The molecular docking analysis showed that compound 3 had better interaction energy (Ei) (-8.59 kcal/mol) and inhibition constant (Ki) values (0.503 μM) than acarbose. These data were supported by MD simulations based on the parameters of RMSD value, the radius of gyration, and protein-ligand interaction energy.

Antimalarial Activity of Sea Sponge Extract of Stylissa massa originating from waters of Rote Island Jefry Presson; Respati Tri Swasono; Sabirin Matsjeh; Meta Permata Putri; Zulfah Az Zahra; Lukas Pardosi
Jurnal Kimia Sains dan Aplikasi Vol 24, No 4 (2021): Volume 24 Issue 4 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Research on the isolation, toxicity test, antimalarial test, and identification of the active compound from the ethyl acetate fraction of Stylissa massa sponge from Oenggae waters, Rote Island, has been conducted. This study aimed to investigate the antimalarial activity of the ethyl acetate fraction of the Stylissa massa sponge. Isolation was carried out by the extraction method using a mixed solvent of methanol: dichloromethane of 3: 2 (v/v), then the extract was partitioned in a solvent mixture of ethyl acetate: water of 1: 2 (v/v). The ethyl acetate extract obtained was separated by column chromatography using the gradient polarity system method. The toxicity test of each fraction was carried out by the Brine Shrimp Lethality Test (BSLT) method, and the antimalarial test was carried out by the haematin polymerization inhibition method. Identification of compounds from the active fraction in the antimalarial test was carried out using Liquid Chromatography-Mass Spectrometry (LC-MS). The extraction yield was 1.14 g (0.23%) of the ethyl acetate extract in the form of a dark brownish-yellow oily solid. Separation by column chromatography resulted in 15 fractions. Toxicity test results showed the four most active fractions with LC50 values, which are very promising for new drug discovery. The IC50 value in the antimalarial activity test of the four fractions indicated that the Stylissa massa sponge ethyl acetate extract was more active than the standard chloroquine compound (115 μg/mL). The LC-MS analysis indicates that fraction 11 contains two compounds that have been reported, and 1 compound is unknown. In contrast, fraction 14 indicates that it contains three compounds that have been reported and one unknown compound.

Anticancer Activity of Venom Protein Hydrolysis Fraction of Equatorial Spitting Cobra (Naja sumatrana) Naseer Ahmed; Garnis Putri Erlista; Tri Joko Raharjo; Respati Tri Swasono; Slamet Raharjo
Indonesian Journal of Chemistry Vol 23, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.80720

Bioactive peptides play an important role in targeting cancer cells. Venom protein from Naja sumatrana can be explored as a source of bioactive peptides. This research aims to identify and study the molecular docking of bioactive peptides (BPs) from trypsin hydrolysate of N. sumatrana venom protein which was fractionated using an SPE C18 column. The venom of N. sumatrana was hydrolyzed with trypsin enzyme. The protein hydrolysate was then fractionated using an RP-SPE HyperSep Retain PEP column, and the peptide fractions were tested for their anticancer activity against MCF-7 breast cancer cells using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Identification of peptides in the active fraction was carried out through high-resolution mass spectrometry. The identified peptides were molecularly docked with the EGFR receptor using AutoDock Vina. The results showed that the degree of hydrolysis was 74.7%. The 75% methanol fraction is the active fraction against MCF-7 cells, with an IC50 value of 4.80 μg/mL and a selectivity index of 5.00. Peptide-active anticancer fractions with the sequence of NSLLVK, SSLLVK and TVPVKR were successfully identified and exhibited high binding affinity values, good RMSD values, and the most suitable model for the epidermal growth factor receptor.

Antiplasmodial and Metabolite Profiling of Hyrtios sp. Sponge Extract from Southeast Sulawesi Marine Using LC-HRMS, Molecular Docking, Pharmacokinetic, Drug-likeness, Toxicity, and Molecular Dynamics Simulation Pulung, Maria Ludya; Swasono, Respati Tri; Sholikhah, Eti Nurwening; Yogaswara, Radite; Primahana, Gian; Raharjo, Tri Joko
Journal of Multidisciplinary Applied Natural Science Vol. 5 No. 2 (2025): Journal of Multidisciplinary Applied Natural Science
Publisher : Pandawa Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47352/jmans.2774-3047.259

Hyrtios sponge is known to possess alkaloid compounds that may exhibit in vitro activity against Plasmodium falciparum. The aim of this study was therefore to isolate and characterise the antiplasmodial active compounds of Hyrtios sp. Sponges collected from the island of Podang-Podang, South Sulawesi, Indonesia. In addition, the LC-HRMS analysis was performed on the active fractions of methanol and ethyl acetate extract to evaluate their antiplasmodial activity. We also validated the in silico antiplasmodial activity of PfDHFR-TS with molecular docking, pharmacokinetics, drug likeness, toxicity, and molecular dynamics analysis. The molecular docking studies showed that the synthesized extremes would have high binding affinity to PfDHFR-TS, thus confirming their potential as powerful enzyme inhibitors. Moreover, the pharmacokinetic and drug-likeness calculations showed that all compounds met the requirements for sufficient resistance and bioavailability, indicating potential as therapeutic candidates. The results of the toxicity analysis indicated that the compounds had a relatively good safety profile, but some potential adverse reactions in the renal and cardiac vasculature could not be excluded. Molecular dynamics simulations confirmed that the complexes formed between the ligand and the target were stable, and the low RMSD value indicated that the active site interactions were also quite stable. These observations reinforce the notion that the extract from Hyrtios sp. not only shows remarkable antimalarial activity but also exhibits pharmacological properties of a prospective drug candidate, which encourages further work in the development of malaria combination therapy both in clinical assessment and comprehensive mechanism of action investigation.

Co-Authors Adita Yuniati Puspitasari Ahmad Saparhadi Eko Sri Kunarti Eko Sri Kunarti Erwin Nur Indiarto Eti Nurwening Sholikhah Fandhi Adi Wardoyo Firmansyah Karim Fitri Rizki Amalia Garnis Putri Erlista Harno Dwi Pranowo Harno Dwi Pranowo Indriana Kartini Indriana Kartini Ira Nur Arba’atul Jannah Jefry Presson Lukas Pardosi Masteria Yunovilsa Putra Meta Permata Putri Naseer Ahmed Norainny Yunitasari Primahana, Gian Pulung, Maria Ludya Sabirin Matsjeh Salim Mustofa Slamet Raharjo Susan Primadevi Syamsul Arifin Tri Joko Raharjo Tri Joko Raharjo Tri Joko Raharjo Tri Rini Nuringtyas Tutik Murniasih Yekti Asih Purwestri Yogaswara, Radite Yosi Bayu Murti Zulfah Az Zahra

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