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INDONESIA
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Published by Pusat Litbang Daya Saing Produk dan Bioteknologi Kelautan dan Perikanan
ISSN : 20895690     EISSN : 24069272     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Squalen publishes original and innovative research to provide readers with the latest research, knowledge, emerging technologies, postharvest, processing and preservation, food safety and environment, biotechnology and bio-discovery of marine and fisheries. The key focus of the research should be on marine and fishery and the manuscript should include a fundamental discussion of the research findings and their significance. Manuscripts that simply report data without providing a detailed interpretation of the results are unlikely to be accepted for publication in the journal.
Arjuna Subject : -
Articles 357 Documents
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Antioxidant and Ace Inhibitor Potential of Stripe Trevally Fish (Selaroides leptolepis) Hydrolysate Reinal Putalan; Ifah Munifah; Tati Nurhayati; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.319

Abstract

This study aimed to investigate the potency of fish protein hydrolysates (FPH) of stripe trevally fish (Selaroides leptolepis) as antioxidant and ACE inhibitor. The FPH was produced through enzymatically hydrolysis using protease produced by Bacillus licheniformis, a collection of Research and Development Center for Marine and Fisheries Product Processing and Biotechnology (RDCMFPPB). The FPH was fractionated using ultrafiltration membranes with molecular weight cut off (MWCO) of 10, 5 and 3 kDa. The hydrolysis degree, protein content, peptide content, antioxidant activity and Angiotensin-converting enzyme (ACE) inhibitor were observed. The result showed that FPH had maximum hydrolysis degree of 63.91% reached after 6 hours hydrolysis with protein content of 27.43 mg/mL and peptide content of 223.32 mg/mL. That FPH showed antioxidant activity (IC50) of 1941.06 ppm and ACE inhibitor of 87.82% at test concentration of 10 mg/mL. In the ultrafiltration step, the higher molecular cut off used, the higher protein content and peptide content. The lower molecular weight of the hydrolysate, the better antioxidant  and ACE inhibitor activity. The best fractionation that produce potential product to be used as anti-hypertension was in mixture peptides between 3-5 kDa. The IC50 antioxidant activity was 1336.96 ppm and percentage of ACE inhibitor was 97.15% % (with a concentration of 10 mg/mL). From the results, protein hydrolysate of stripe trevally fish produced by enzymatic hydrolysis using local protease was potential as a functional ingredient, particularly as antihypertensive agent.
Liquid smoke and its applications for fisheries products Diah Lestari Ayudiarti; Rodiah Nurbaya Sari
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 3 (2010): December 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v5i3.53

Abstract

Liquid smoke is a volatile compound that simultaneously evaporates from heat reactor throughpyrolization and condense in cooler. Liquid smoke was produced in several steps that arepyrolization, condensation and redistillation. Quality, composition and material in liquid smoke isinfluenced by wood type as raw material. The major component of liquid smoke are acid, fenolderivate and carbonil. Those components can be used as flavoring, coloring, antibacterial andantioxidant agents. Liquid smoke can be used as preservative because of its antibacterial andantioxidant activity. Fenol and acetic acid compounds in liquid smoke can inhibit bacterial growthsuch as Pseudomonas fluorescence, Bacillus subtilis, Escherichia coli and Staphylococcusaureus. Fenol also can be used as antioxidant by stabilizing the free radicals. Liquid smoke cangive specific flavor and better color in smoke products. Liquid smoke can be applicated in eel, fishor products diversification such as fish steak. Liquid smoke also can be used in agricultural andtimber industry.
Preface Squalen Bulletin Vol. 12 No. 2 Tahun 2017 Squalen Squalen
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i2.309

Abstract

Performance of bioreactor and pH meter instrument in bioethanol producing process from brown seaweed(Sargassum duplicatum) Rodiah Nurbaya Sari; Diah Lestari Ayudiarti; Diini Fithriani
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 6, No 2 (2011): August 2011
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v6i2.64

Abstract

To meet the energy needs this time we are still dependent on energy derived from fossilresources that can not be recovered (fossil energy). The way to tackle the energy problem is toexplore other energy resources namely biofuels, one of its kind is bioethanol. Source of potentiallyraw material from the sea is brown seaweed which cellulose content and stored carbohydrate(mannitol) is quite high. The use of a batch bioreactor and the pH meter instrument assembliesResearch Center for Marine and Fisheries Products Processing and Biotechnology (RCMFPPB)has been supporting the production of bioethanol with raw material brown seaweed Sargassumduplicatum.
BACTERIAL DIVERSITY OF THE DEEP SEA OF SANGIHE TALAUD, SULAWESI Gintung Patantis; Ekowati Chasanah; Dewi Seswita Zilda; Ikhsan B. Waluyo
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 7, No 1 (2012): May 2012
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v7i1.12

Abstract

Deep sea is an extreme environment characterized by cold temperature, high pressure, lackof  light and nutrients. Microorganisms live in these habitat are unique microorganisms andknown to have tremendous source of potential agents for biotechnology processes. Indonesia asan archipelagic country has a vast deep ocean. This study aims to see the diversity of bacteria inSangihe Talaud Deep Sea, Sulawesi. Analysis of bacterial diversity was carried out by culturedand uncultured method. Terminal Restriction Fragment Length Polymorphism (T-RFLP) techniquewas used for uncultured analysis of the microorganisms biodiversity, while cultured one wasdone by plating the samples of water onto Zobell media. The results showed that, there were 21isolates obtained by cultured method. The identification which based on 16S rDNA by PCR methodshowed the genus of Pseudomonas, Pseudoalteromonas, Alteromonas, Vibrio, Shewanella andUncultured bacterium were identified. However, 14 classes of bacteria were obtained by usingTRFLP method i.e Acetobacteraceae class, Actinobacteria, α-proteobacteria, -proteobacteria, δ-proteobacteria, γ-proteobacteria, Bacili, Bacteroidetes, Chlorobi, Chroococcales, Clostridia,Erysipelotrichi, Synergistia, and Zetaproteobacteria. here were also  unclassified bacteria anduncultured bacterium found in the samples.
TEKNIK IDENTIFIKASI MIKROORGANISME SECARA MOLEKULER Gintung Patantis; Yusro Nuri Fawzya
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 4, No 2 (2009): August 2009
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v4i2.146

Abstract

Akhir-akhir ini bioprospeksi mikroorganisme laut semakin populer dan banyak  diminati karena potensinya yang menjanjikan sebagai sumber komponen bioaktif baru. Identifikasi mikroorganisme merupakan salah satu tahapan yang penting dalam bioprospeksi. Perkembangan identifikasi mikroba diawali dengan identifikasi melalui ciri-ciri morfologi, fisiologi, dan metabolisme. Namun adanya kekurangan-kekurangan metode ini yaitu berupa ketidakakuratan dan waktu identifikasi yang lama menjadikan metode secara molekuler lebih berkembang. Pada bakteri, 16S ribosom deoxyribonucleic acid (rDNA) mempunyai daerah sekuen yang konservatif sehingga dapat digunakan untuk menduga hubungan kekerabatan secara alami  antar  spesies. Sedangkan pada kapang digunakan 18S rDNA dan daerah internal transcribed spacer (ITS) untuk identifikasinya. Tahapan identifikasi dengan metode molekuler meliputi ekstraksi deoxyribonucleic acid (DNA), amplifikasi DNA, sekuensing, analisis hasil sekuen, dan pembuatan pohon filogenetik. Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan (BBRP2B-KP) memiliki  koleksi mikroba potensial penghasil enzim kitosanase, kitinase, dan protease dari berbagai sampel dari lingkungan laut. Berdasarkan pohon filogenetik  beberapa isolat koleksi memiliki kemiripan 87–96% dengan Staphylococcus caprae, Stenotrophomonas maltophilia, Acinetobacter sp., Bacillus licheniformis, Geobacillus stearothermophilus.
Back Cover Squalen Bulletin Vol. 10 No. 1 Tahun 2015 Squalen Bulletin
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 10, No 1 (2015): May 2015
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v10i1.338

Abstract

Chitosan Composite of Crab Shell and Hydroxyapatite of Tuna Fish Bone as Biomaterials for Guided Tissue Regeneration Thamrin Wikanta; Erizal Erizal; Sugiyono Sugiyono
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 3 (2013): December 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i3.85

Abstract

The needs of membrane biomaterials to prevent the migration process of epithelial cells into the bony area after tooth withdrawal process, for filler the holed tooth and to cure gingivitis have increased significantly. In this study, a biomembranes composed of chitosan (CTS) and hydroxyapatite (HA) were prepared. CTS was made of crab shell and HA was made of tuna fish bone. There were 4 groups of treatments, i.e.: K0: only 4 g CTS as a control; K1: combination of 4 g CTS and 2 g HA; K2: combination of 4 g CTS and 4 g HA; and K3: combination of 4 g CTS and 6 g HA. The dried and solid membrane was soaked in 5% Na2SO3 for 1 hour, and then dried in oven at 60 0C for 24 hours. The composite membrane properties, i.e.: water absorption, biodegradation, tensile strength, elongation at break, functional group (by FTIR), and microstructure (by SEM) were evaluated. Result showed that the increasing of HA content in membrane will decreasing the water absorption. The increasing of HA content in membrane and soaking time in Phosphate Buffered Saline (PBS), can increase in biodegradation rate. The biodegradation rate of membrane was about 4.44-6.01% during 27 days of soaking time. The increasing soaking time in PBS can decrease the tensile strength of membrane at about 18.33%-21.17% and decreased the elongation at break at about 26.64%-59.94%. FT-IR analysis indicated that there was a crosslinking between a CTS-HA composite membrane and Na2SO3. Among all the composites studied, CTS-HA (4/2 g/g) composite membrane is the best one for Guided Tissue Regeneration (GTR).
Cembranoids Content of Soft Coral Sarcophyton from Acidified Environment at Volcano Island, Indonesia Hedi Indra Januar; Neviaty Putri Zamani; Dedi Soedharma; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.276

Abstract

Cembranoid content in soft coral is known as a chemotype that relate with genotype and environment. This research aimed to characterize the cembranoid Sarcophyton soft coral from the reef that acidified by CO2 volcanic vents (pHT 7.8) at Volcano Islands waters, Banda-Neira (Indonesia), as a means of predicting the future impact of ocean acidification to the genetic diversity of Sarcophyton soft coral. 30 random colonies were taken, combined, and extracted with ethanol. Cembranoid isolation and identification had been done by high performance liquid chromatography and spectroscopic techniques. Results of the study found sarcophytol derivatives (sarcophytol A, 11,12-epoxy sarcophytol A, sarcophytol B, and sarcophytol M) as the only chemotype in the sample. This may suggest low genetic diversity in the observed Sarcophyton sample. Therefore, it may suggest that even soft coral is known to be resilient to future acidification pressures, the genetic diversity or the production of diverse cytotoxic metabolite may be hampered due to ocean acidification in future climate change adaptation.
Liquid organic fertilizer from seaweed (Sargassum sp.)and fish waste hydrolysate Jamal Basmal
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 5, No 2 (2010): August 2010
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v5i2.48

Abstract

The combination of seaweed Sargassum sp. and fish waste hydrolysate can be used as anorganic fertilizer, both in liquid or solid forms. The organic fertilizer has a complete macro andmicro nutrients. It contains growth hormone factor such as auxin, cytokinine (zeatin and kinetin)and giberelin. The function of growth hormone factor in plants is to enhance the roots, stems, andleaves expansion, as well as to increase fruit production. To produce liquid organic fertilizer,seaweed has to be hydrolyzed in alkaline condition, meanwhile solid waste of fish has to behydrolyzed in acid condition. The liquid organic fertilizer had protein value of 0.08% or equal to0.012% N, 0.13% P, 1.22% K, 0.06% Ca, 0.17% Mg, 55.04% Fe, 122.75 ppm Mn, 1.95 ppm Cu,24.59 ppm Zn, 13 ppm B, and 18% alginic acid. It had auxin (IAA) of 91.48 ppm, cytokinine of84.71 ppm kinetin and of 70.27 ppm zeatin and giberellin (GA3) of 107.72 ppm.

Page 7 of 36 | Total Record : 357

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