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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Comparison of the Reduction Effect of Sucrose and Table Sugar Concentration on Growth Characteristics of Red Ginger (Zingiber officinale Rocs.) Cultured in Liquid Medium Hapsari, Betalini Widhi; Ermayanti, Tri Muji; Rantau, Deritha E.; Rudiyanto, Rudiyanto
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/34

Abstract

The aim of this research was to compare the reduction effect of sucrose or table sugar concentration on growth characteristics of red ginger cultured in MS liquid medium. Shoots of red ginger was cultured on MS liquid medium without addition of plant growth regulators, supplemented with 5, 10, and 20 g/l of sucrose or table sugar for 8 weeks. Resulted plantlets were acclimatized in a greenhouse to investigate their growth and survival rate. Numbers of stomata, chlorophyll concentration as well as cross section of leaves from plantlets grown in vitro were compared to those of transplants grown in the greenhouse. The results showed that the use of table sugar at concentration of 20 g/l gave the best growth of red ginger. Meanwhile, the reduction of table sugar from 20 to 10 g/l reduced growth and survival rate of in vitro shoots as well as that of transplants in the greenhouse. Only few shoots formed roots when they were grown on the medium containing 5 g/l of table sugar, and transplants failed to grow in the greenhouse. It found that the chlorophyll content of in vitro plantlets was lower than those of transplants grown in the glasshouse. However, the number of stomata of the in vitro plantlets was higher than that of transplants grown in the glasshouse. There was no anatomical abnormalities found on the cross section of leaves between in vitro plantlets and transplants grown in the greenhouse. The replacement of sucrose with table sugar may reduce the production cost of plantlets.Keywords: red ginger (Zingiber officinale Rocs.), reduction of sugar, survival rate, chlorophyll, stomata.
Editors Preface AB Vol 20 No 1 (2016) Anugerah, Muhamad Dzikri
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i1.262

Abstract

Isolation of Endophytic Microbes from Gunung Halimun National Park, West Java, Indonesia and Bioassay Their Potency for Eradicating Microbial Crops Pathogen Sukara, Endang; Melliawati, Ruth
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/73

Abstract

Gunung Halimun National Park (GHNP) – West Java, Indonesia is the largest preserved primary forest in West Java. Diversity of plants and animals of this park have been studied intensively during the 15 years years. Diversity of endophytic microorganisms, however, has never been reported. Endophytic microbes are those who reside in the interspatial tissues of plants, have a capacity to produce array of secondary metabolites. This paper illustrates the occurrence of endophyte microbes in diverse flowering plants of GHNP. Total of 160 bacteria and 337 fungus were isolated from 86 plants species in the area. Out of that, 159 bacterial isolates and 320 fungus isolates survived in our collection and tested against four major microbial crops pathogen namely Xanthomonas campestris, Pseudomonas solanacearum, Colletotrichum glocosporoides and Fusarium oxysporum cubense. Plate Agar Test Assay method reveals that 51 among 159 bacterial isolates and 62 among 320 endophytic fungal isolates have an ability to inhibit the growth of microbial crop pathogens. Endophytic bacteria can inhibit more microbial crops pathogen but the inhibition ability is less compare to that of endophytic fungi. From our preliminary study clearly shows that endophytic microbes of GHNP should have potential value in developing biological control agent to combat microbial crop pathogens and eventually reduce the use of synthetic chemicals.
Identification and Activity of The Retrotranposon Tos17 in Indonesian Javanica Rice CV.Rojolele And Japonica Rice CV. Gajahmungkur Nugroho, Satya; Loedin, Inez HS; Ouwerkerk, Pieter BF
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2649.41 KB) | DOI: 10.1234/20

Abstract

Retrotransposons arc  mobile  genetic  elements  that  transpose  via  an  RNA intermediate  that  is  reverse transcribed  before  integration  into  a  new  location  within  the  host  genome. They  are  ubiquitous  in  eukaryotic organism.  and constitute a major portion of the nuclear genome (often more than half of the total DNA) in plants .Tos17 is a  rice  endogenous  retrotransposon  on  that  has  been  studied  thoroughly. Tos17 has  been  shown  to  be  an efficient insertional mutagen and saturation mutagenesis tool for gene tagging and functional genomics in Japonicarice cv  Nipponbare.  In  lavanica rice however, the presence and activity of  Tos17 has not been described thus far. while in some Indica rice Tos17 has  been found  to be  inactive. Javanica rice, also known as tropical  Japonica rice,has many cultivars which may serve as pontetial genetic resources of great interest for breeding programmes. Here  , the presence and activities of retrotransposon Tos17 in  Javanica rice  cv Rojolele was described and compared  to those of Japonica rice and Japonica rice cv Gajahmungkur We  identified  five and theree copies of  Tos17 in Rojolele and Gajahmungkur, respeclively, with different activities.Keywords: Oryza  sativa, rice, javanica, retrotransposon, Tos17
Front Cover AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (774.26 KB) | DOI: 10.14203/ab.v21i1.303

Abstract

Site-Directed Mutagenesis of Glu-269 L-Arabinose Isomerase from Geobacillus stearothermophilus Isolated from Tanjung Api Poso, Indonesia Fitriani, Dewi Fitriani; Wulandari, Puspita Suci; Saksono, Budi
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.974 KB) | DOI: 10.1234/55

Abstract

Industrializing of tagatose requires enzymes that meet to industrial need such as thermophile, slightly acidic and metal independent.  Previously,  we  cloned,  sequenced  and  expressed  L-arabinose  isomerase  from  Geobacillus stearothermophilus  isolated  from Tanjung  Api, Poso, Indonesia. Based on DNA alignment analysis, the gene had  high  homology  with  those  of  G.  stearothermophilus  T6  (Gene  Bank  Acc  No:  AAD45718)  which  has optimum  activity  at  high  temperature  and  alkaline  condition.  In  this  p aper,  we  described  site-directed mutagenesis approach to mutate Glu-269 (Q269) to Lys-269 (K269) to decrease the optimum pH of the strain. Sequencing  result  showed  that  mutagenesis  had  been  successful  to  mutate  amino  acid  at  position  269  from glutamine (Q) into lysine (K). Expression of mutant Q269 showed protein with molecular mass ~56 kDa. Keywords: site-directed mutagenesis, Q269K, L-arabinose isomerase, Geobacillus stearothermophilus
Development of Somatic Embryo in Lithospermum erythrorhizon Siebb. et Zucc and The Study on The Effect of Methyl Jasmonate on its Maturation Mariani, Totik Sri; Ramayanti, Octavia; Yazaki, Kazufumi; Miyake, Hiroshi
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3351.663 KB) | DOI: 10.1234/10

Abstract

A research on the somatic embryogenesis in Lithospermum erythrorhizol1 has been conducted. Embryogenic callus was inoculated in EIM9 liquid medium (modification ofLS medium), i.e. L2PVP (initiation medium) and the development of somatic embryo was observed. Ten J.1M and 100).lM methyl jasmonate was added into L3PVP medium (differentiation medium) for somatic emb yo maLUration. The purposes of this research were to observe tlle development of somatic embryo and to observe the effect of methyl jasmonate on maturation of the somatic embryo in L erylhrorhizol/ . The results showed that somatic embryogenesis in L erythrorhizon derived from single cells differentiated further forming proembryo, globular. heart, torpedo and cotyledon stage. Treatment with 10 f.1M and 100).lM methyl jasmonate induced maturation of somatic embryos (cotyledon stage) after transferring them to embryo development medium (L4PVP).
Identification of a New Compound as α-Glucosidase Inhibitor from Aspergillus aculeatus Dewi, Rizna Triana; Suparman, Asep; Mulyani, Hanny; Darmawan, Akhmad; Lotulung, Puspa Dewi Narrij
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i1.213

Abstract

Terestrial fungi are of great importance as potential sources of pharmaceutical agent. Aspergillus aculeatus, a fungus isolated from soil sample collected in Indonesia, was cultured in liquid media to investigate a novel compound as inhibitor α-glucosidase. The mycelium extract of A. aculeatus shows potential activity against Saccharomyces cereviseae α-glucosidase and mild activity against mammalian α-glucosidase with IC50 values of 9.57 µg/mL and 470.76 mg/mL, respectively. Enzyme assay-guided fractionation of this extract afforded rubrofusarin (1). Rubrofusarin, a linear naphtho-γ-pyrone, is a natural pigment from Aspergillus sp. Interestingly, compound 1 shows potential inhibitory activity against mammalian α-glucosidase (IC50 of 92.7 µg/mL), but no to S. cereviseae α-glucosidase. The results suggest that A. aculeatus is a promising natural source as a lead compound in the discovery of antidiabetic drug.
ILE655VAL Genotyping Study of HER2 - Positive Breast Cancer of Patients from Padang - Indonesia With High Resolution Melting Technique Wulandari, Dwi; Azamris, Azamris; Nurhayati, Isna; Warisman, M Ali; Budiarto, Bugi Ratno; Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.104 KB) | DOI: 10.14203/ab.v21i2.310

Abstract

Transtuzumab has proven to be a great improvement in the treatment of HER2 + of breast cancer patients, but it is associated with relevant adverse cardiac events and with significantly elevated cost of treatment.  One of the risk factors for cardiotoxicity due to transtuzumab is the I655V HER2 polymorphism (GTC> ATC mutation) in which the allele mutant (Ile val or val / val) has a higher risk than the wild type (Ile/Ile). The detection of specific alleles is very important for therapeutic decision-making. In this study, our group have developed a HRM with EvaGreen dye method to discriminate specific allele of I655V HER2 (wild type, heterozygote mutant or homozygote mutant) in 66 frozen section samples of HER2+ of breast cancer patients. Our group reported the wild type is the most prevalent allele (77,27%), whereas heterozygous mutation is significantly present in this research (21,21%) and only 1,52% of sample detected as minor allele.  It showed that only one sample detected as a minor allele (val/val) and may have relatively low abundance in the population. This method has been compared with sanger sequencing and giving 100% of validity.
Generation of mCherryBody: an Anti-Transferrin Receptor Antibody Variable Fragment Linked by The Fluorescent Protein mCherry Kusharyoto, Wien; Andriani, Dian; Handayani, Ira
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v20i2.233

Abstract

A facile generation of a recombinant antibody fragment with intrinsic fluorescent properties of the monomeric fluorescent protein mCherry is described. The so-called mCherryBody was designed based on the structure model of the variable fragment of anti-transferrin receptor antibody LUCA31 and the X-ray crystallographic structure of the protein mCherry. mCherryBody was constructed to retain optimal spatial geometry between the C- and N-termini of the antibody light-chain (VL) and heavy-chain (VH) by mimicking the domains interface pairing in antibody Fab fragments and incorporation of the fluorescent protein mCherry as a bridging scaffold. The gene encoding the chimeric protein was cloned into the pJExpress414 expression vector, expressed and secreted into the periplasm of Escherichia coli NiCo21(DE3) for assembly and disulphide bond formation. Based on its amino acid sequence, mCherryBody was predicted to have a molecular weight of 51.46 kDa. The modular assembly used in the generation of mCherryBody may permit the interchange of binding sites and of fluorescent proteins to create robust panels of coloured antibody fragments. Thus, the mCherryBody platform facilitates rapid generation of colored single-chain variable fragment (scFv) chimeras that could be used for screening of antibodies against cell surface markers or receptors.

Page 11 of 54 | Total Record : 540

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