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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan Matematika dan IPA AGRIVITA, Journal of Agricultural Science Sawwa: Jurnal Studi Gender ANNALES BOGORIENSES AGROTEK: Jurnal Ilmiah Ilmu Pertanian Jurnal Sains dan Teknologi Pertanian Makara Journal of Health Research Jurnal Ekonomi, Manajemen, Akuntansi Jurnal El-Thawalib Annales Bogorienses Journal of Islamic Banking, Economics and finances (JIBEF)

Desriani Desriani

Research Centre for Biotechnology, LIPI. Jl. Raya Bogor Km 46, Kec. Cibinong, Kab. Bogor, 16911. Indonesia. Telp: +62-21-8754587.

Author-ID : 251653

Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Social Sciences

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Editorial Boards AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Editors Preface AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Guide for Authors AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Improvement of HER2I655V TARMS-PCR Performance by DNA Quality Analysis Budiarto, Bugi Ratno; Azamris, Azamris; Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v21i2.308

Reliable TARMS-PCR is a prerequisite in constructing a solid conclusion in genetic diagnostics. The validity of data generated by this molecular technique is hampered by a false positive result. In attempt to develop aÂ TARMS-PCR for HER2I655V genotyping with no interfering of bias we used DNase I to eliminate DNA contaminant resided in PCR reagent. TARMS-PCR without enzyme treatment using recombinant plasmids that contained HER2I655V gene with represented its alleles was used to evaluate the presence of false positive Â result while DNase I treated-PCR reagent was used in TARMS-PCR to evaluate the effective dose of the enzyme and further to adjust the TARMS-PCR conditions.Â PCR master mix kit used in this study produced a false positive result on HER2I655V TARMS-PCR as proven by the presence of multiple PCR products in Non-Template Control (NTC) and 0.1 U of the enzyme could eliminate this DNA contaminant effectively, although this pretreatment altered the specificity of HER2I655V TARMS-PCR genotyping on certain genotype. Combination of touchdown TARMS-PCR with another allele-specific primer recovered specificity of detection on this model system. Interestingly, this optimized HER2I655V TARMS-PCR can only be used for genotyping the clinical samples if only further optimization was done using genomic DNA as template

Front Cover AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

ILE655VAL Genotyping Study of HER2 - Positive Breast Cancer of Patients from Padang - Indonesia With High Resolution Melting Technique Wulandari, Dwi; Azamris, Azamris; Nurhayati, Isna; Warisman, M Ali; Budiarto, Bugi Ratno; Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Transtuzumab has proven to be a great improvement in the treatment of HER2 + of breast cancer patients, but it is associated with relevant adverse cardiac events and with significantly elevated cost of treatment.Â One of the risk factors for cardiotoxicity due to transtuzumab is the I655V HER2 polymorphism (GTC> ATC mutation) in which the allele mutant (Ile val or val / val) has a higher risk than the wild type (Ile/Ile). The detection of specific alleles is very important for therapeutic decision-making. In this study, our group have developed a HRM with EvaGreen dye method to discriminate specific allele of I655V HER2 (wild type, heterozygote mutant or homozygote mutant) in 66 frozen section samples of HER2+ of breast cancer patients. Our group reported the wild type is the most prevalent allele (77,27%), whereas heterozygous mutation is significantly present in this research (21,21%) and only 1,52% of sample detected as minor allele. Â It showed that only one sample detected as a minor allele (val/val) and may have relatively low abundance in the population. This method has been compared with sanger sequencing and giving 100% of validity.

Back Cover AB Vol 21 No 1 (2017) Desriani, Desriani
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The Sex Education Method in Agrarian Communities Desriani, Desriani; Muthohar, Sofa; Filasofa, Lilif Muallifatul Khorida; Mursid, Mursid
Sawwa: Jurnal Studi Gender Vol 16, No 1 (2021): April
Publisher : Pusat Studi gender dan Anak (PSGA) Universitas Islam Negeri Walisongo Semarang

The purpose of this study was to describe how an agrarian society provides sex education. This research was conducted based on the assumption that parents need to teach sex education to early childhood, even though it is taboo, but in a good way. This research is qualitative research with an ethnographic research type. The results showed that 1) The community uses fiqh (rituals of worship) learning methods to cover genitalia and teach the culture of shame from an early age. 2) people are still taboo to mention genitals directly so that they use other terms that are considered not to stimulate crime and are more polite, namely to refer to the penis as sunik, gentog and anu, while for the vagina is replaced with the term memek, iwak kebo and anu. 3) people still use the circumcision method for women even though WHO has stated it is prohibited. This study recommends the government and community leaders to continue to carry out sex education for children with the correct method according to health, religious, and human rights laws.

Development of Sybr Green I-Based Melting Curve Method for HER2I655V Polymorphism Detection in Breast Cancer Budiarto, Bugi R; Harahap, Wirsma A; Desriani, Desriani
Makara Journal of Health Research Vol. 20, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Currently available molecular method to detect HER2I655V polymorphism such as PCR-RFLP is hampered by the costly experimental method, and post-PCR treatment requirement that makes this technique is not meeting for high-throughput analysis purpose. In this study, we developed an accurate, simple, low cost and rapid test to detect polymorphism at HER2 gene using SBR Green I based-melting curve method. Methods: Two forward allele-specific primers and one common reverse primer were used then these primers were tested to discriminate known genotypes of genomic templates (GG type or AA type) and genomic samples retrieved from breast cancer patients. Results: Melting curve analysis derived from SYBR Green I-based allele-specific PCR with defined primers concentration and annealing temperature at 54.3 °C showed good discrimination level of Tm peaks in which GG genotype melted at 89 °C slightly higher than AA genotype which melted at 86 °C, while AG genotype harbored both of homozygous Tm characteristics. Conclusions: This preliminary result will be as basic for further large-scale typing of HER2I655V polymorphism.

TA CLONING PARTIAL ALBUMIN GENE AND ITS TEST FOR SUPPORTING PHARMACOGENOMIC DETECTION DEVELOPMENT Effendi, Rinal; Putri Kumala, Ajeng; Marti Abna, Inherni; Yurika, Oktri; Desriani, Desriani
Jurnal Pendidikan Matematika dan IPA Vol 15, No 3 (2024): September 2024
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jpmipa.v15i3.85093

DNA recombinant technology is a tool that could support thedevelopment of pharmacogenomic detection such as for CYP2D6 copy number detection application. The housekeeping gene was identified as an internal control alternative for the copy number of the CYP2D6 detection component. There are many kinds of housekeeping genes, including albumin. In this report, we have successfully TA-cloned partial albumin to support the development of CYP2D6 copy number detection. TA-cloning was successfully confirmed with white-blue methods in media LB + ampicillin + IPTG + X-Gal and with colony PCR methods. Further, we made a standard curve with this recombinant plasmid to evaluate albumin qPCR efficiency and showed high-efficiency qPCR 0,9914 (99%). Based on the CYP2D6 copy number developed, the test in this study showed low concordance with high reproducibility Long PCR, indicating that the nucleotide base area of albuminand CYP2D6 in this studywas not recommended for CYP2D6 gene multiplication detection.

Co-Authors Abna, Inherni Marti Adi Santoso Agustiyanti, Dian Fitria Andri Wardiana, Andri Atikana, Akhirta Azamris, Azamris Azamris, Azamris Azzania Fibriani Baehaqi Budiarto, Bugi R BUGI RATNO BUDIARTO Chaeriyah, Naeilul Dwi Endah Kusumawati Dwi Wulandari Dwiana Indah Lestara Edi Haryono ERNAWATI ARIFIN GIRI-RACHMAN Ferry Iskandar Filasofa, Lilif Muallifatul Khorida Fitri Fitri Hapsari, Betalini Widhi Harahap, Wirsma A Hariyatun, Hariyatun Hasibuan, zulfan Efendi Haz, Aufa Rizqia Isna Nurhayati, Isna Jumardi Jumardi Kumala, Ajeng Putri Kusharyoto, Wien LINDA SUKMARINI MARIA BINTANG Marissa Angelina Muhammad Adriani, Muhammad Munawarah Munthe, Marabona Mursid Mursid Neneng Hasanah Neng Herawati, Neng Noorohmah, Siti Okasa, Andi Muliarni Permatasari, Fitri Aulia Popi Hadi Wisnuwardhani, Popi Hadi Prasetyoputri, Anggia PUSPITA LISDIYANTI Putra, Masteria Yunovilsa Putri Kumala, Ajeng Rahayu, Resa Sri Raihan, Eldrian Daffa Rantau, Deritha Ellfy RATIH ASMANA NINGRUM Rinal Effendi Risalan Basri Harahap Riyona Desvy Pratiwi, Riyona Desvy Siti Zubaidah Sitti Fatimah Syahid Sofa Muthohar Stephanie, Rebecca Sulaiman Sulaiman Syamsul Falah Taharuddin, Audrey Angelina Putri Wahyudi, Denny Warisman, M Ali Warisman, M. Ali Wiwit Amrinola Wulandari, Dyah Retno Yamahoki, Nicholas Yana Rubiyana, Yana Yuliawati Yurika, Oktri

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