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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Rapid Purification and Partial Characterization of an Extracellular Enantioselective Lipase from Aspergillus niger Dominggus Malle; Gina Garingan; Milagros Peralta; Maria Jamela Revilleza
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3937.958 KB) | DOI: 10.14203/ann.bogor.2008.v12.n1.1-8

Abstract

Lipases  [EC.  3.1.1 .3]  are  capable  of hydrolyzing  ester  bonds  of triglycerides  and  have  received  much attention  from  protein  researchers  after  these  enzymes  have  shown  potential  applications  such  us,  in  the manufacture of commercially important chiral drugs due to their enantioselectivity properties. A novel  extracellular lipase  produced  by Aspergillus  niger was  purified  and  partially  characterized.  Using  the  culture medium  as crude extract, the Lipase was purified  to 25 .7  folds after Ultrafiltration and DEAE ion exchange chromatography. The enzyme was characterized to have optimum activity at neutral pH and at 30-35°C, while its kinetic parameters, Vmax and Km, were determined  to  be  L80 U/mL/min  and 0.2 mL , respectively. SDS-PAGE analysis  revealed  the presence of at  least two  bands  (±87  and ±72  kDa), while  native  PAGE  showed  a  single  band.  The  two  bands could represent subunits  in  a complex or isoforms of the enzyme. The use of  the enzyme  in  th  hydrolysis of a racemic  mixture  of 2-arylpropionic  butyl  ester  analyzed  after HPLC demonstrated  an  apparent predominant enantioselectivity for the S (+) enantiomer. Thus, this lipase is a promising enzyme for the chiral drug preparation of  single enantiomer of 2-arylpropionic acid (ibuprofen) .Keywords: Iipase, Aspergillus niger, purification, enantioselectivity, chiral  drug, ibuprofen
Guide for Authors AB Vol 19 No 1 (2015) Muhamad Dzikri
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.403 KB) | DOI: 10.14203/ann.bogor.2015.v19.n1.%p

Abstract

Present Status and Challenges on Agricultural Microbe Bio-Prospecting in Indonesia Endang Sukara
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4484.648 KB) | DOI: 10.14203/ann.bogor.2007.v11.n1.31-39

Abstract

food security is  one of the essential needs which can never be  ignored by any society,  including in particular Indonesia. as the world population is expected to rise to around  10 billion marked by 2025. The problem  is that the agriculture in developed and developing countries that  follow the path of developed countries experiences create · a  range of  serious environmental degradation. An  intensive agricultural production through the use of pesticide and herbicides, forest clearing and continued use of land resources  for the agricultural purposes are not sustainable practice  .  In  consequences,  degradation  of ecosystem,  oil  erosion.  and  low  productivity  of land,  poor water quality and lost of biological  diversity  (including microbial  and  genetic  resources) occurred  at alarming  stale. Meanwhile,  the efforts  in  addressing  the above  situation  is  traditionally  focused on plant phenotypes (breeding. plant genetic engineering etc .) but the important role of microbial communities that interact with plants to influence plant health and productivity has been  largely ignored. The  interaction of microbes with plants and  its  relevant to soil  fertility and productivity, the bio-control properties of microbes and challenges toward sustainable agriculture is highlighted.
Editor's Preface AB Vol 17 No 2 (2013) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.274 KB) | DOI: 10.14203/ann.bogor.2013.v17.n2.%p

Abstract

Front Cover AB Vol 14 No 2 (2010) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2010.v14.n2.%p

Abstract

Diversity and Antimicrobial Activity of Lichens-Associated Actinomycetes in Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) Indonesia Agustina Eko Susanti; Shanti Ratnakomala; Wibowo Mangunwardoyo; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (945.612 KB) | DOI: 10.14203/ann.bogor.2019.v23.n1.1-12

Abstract

    Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552.
Back Cover Vol 9 No 1 (2004) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 9, No 1 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2004.v9.n1.%p

Abstract

Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1-0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1-0031 Elvi Yetti; Ahmad Thontowi; Yopi Yopi
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.12 KB) | DOI: 10.14203/ann.bogor.2017.v21.n2.38-44

Abstract

Dibenzothiophene (DBT) and its derivatives have been widely used as model organic sulfur compounds in petroleum, included their biodegradation process. The abilities of microorganisms to degrade pollutants are significantly influenced by various factors such as microbial species, nutrients and environmental parameters. In this research, we carried out further study to determine optimal condition for DBT biodegradation regarding with substrate and strains cell concentration by several indigenous marine bacteria from Indonesia. These three isolates were belong to Mauricauda olearia, Alcanivorax xenomutants, and Stakelama pacifica, with homology result 99% each. Optimal dibenzothiophene as substrate reached by all isolates is 100 ppm, while cell concentration or microbial numbers that gave highest growth for all isolates is 20 based on conversion of OD600 nm measurement.  
Pcr Amplification of Ornithine Decarboxylase (ODC) Gene Fragment from Tobacco (Nicotiana tabacum L.) cv. Temanggung Ira Djajanegara; Sabar Pambudi; Retno Lestari; Nina Artanti
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2606.14 KB) | DOI: 10.14203/ann.bogor.2004.v9.n2.80-86

Abstract

In order Lo create an antisense construct of the gene encoding Ornithine Decarboxylase (ODC) from tobacco (Nicotiana tabacum L.) cv. Temanggung, the target gene must be isolated. In this paper. we present the PCR amplification of a fragment from putative gene encoding ODC from tobacco cv. Temanggung. Leaf genomic DNA was i olated and used as the template for PCR. PCR optimization was done by adjusting the annealing temperature and the cycle number. Verification of the fragment obtained was also done using the second primer pairs .  
Editorial Boards AB Vol 16 No 2 (2012) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 16, No 2 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (430.723 KB) | DOI: 10.14203/ann.bogor.2012.v16.n2.%p

Abstract

Page 30 of 54 | Total Record : 540

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