Garuda - Garba Rujukan Digital

All

ANNALES BOGORIENSES

annales
Website

Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 05178452 EISSN : 24077518 DOI : -

Core Subject : Health, Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.

Arjuna Subject : -

Articles 540 Documents

30 31 32 33 34

Guide for Authors AB Vol 20 No 2 (2016) Muhamad Dzikri
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Genetic transformation of rice cv. Ciherang using double T-DNA vector harboring cry1Ab gene Yuli Sulistyowati; Agus Rachmat; Fatimah Zahra; Syamsidah Rahmawati; Satya Nugroho
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Rice stem borer (Scirpophaga sp.) is considered as one of the primary pests causing yield decrease in Indonesia. Genetic engineering is considered as one of the most effective way to improve the resistance of rice plants to yellow stem borer since no resistance gene has been found in rice and its wild relatives. A double T-DNA binary vector p2TDNAcryIAb wich carry two independent T-DNA was used to transform Indonesian elite rice cv Ciherang using Agrobacterium-mediated transformation to generate transgenic rice that free from selectable marker genes. One T-DNA containing a selectable marker hygromycin phosphotransferase (hpt) gene and the other carrying the cry1Ab gene. As many as 37 putative transgenic plants from 14 embryos were generated. PCR analysis demonstrated that 22 out of 37 putative transgenic rice contain cry1Ab gene. All Ciherang rice carrying the cry1Ab gene expressing Cry1Ab protein as shown by the immunostrip assay. Further work will be carried out to determine the transgenes copy number, to select marker free transgenic rice, and to evaluate the resistance of transgenic rice against neonates larvae of yellow stem borer.Keyword: double T-DNA, transgenic rice, Cry1Ab, transformation

Optimizaton of Cationic Lipid Mediated Transfection of pEGFP-c1 and pJ-EPO Plasmids in Chinese Hamster Ovary (CHO) Cells Attached Culture for Transient and Stable Recombinant Human Erythropoietin (rhEPO) Expression Endah Puji Septisetyani; Arizah Kusumawati; Adi Santoso
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Cationic lipid is one of transfection agents which show high efficiency and low cytotoxicity. The transfection efficiencies may depend upon the type or amount of cationic lipids, the cell line or DNA plasmid being used for transfection. The purpose of this study was to find optimal condition for transfection of CHO-K1 and CHO-S cells with pJ-EPO plasmid (contain human epo gene) compared with pEGFP-c1 plasmid (contain gfp gene) by cationic lipid Lipofectamin 2000TM to generate stable transfectant expressing recombinant human erythropoietin (rhEPO). Optimization was carried out regarding the amount of lipofectamin, DNA concentration, and concentration of antibiotic Geneticin (G418) for selection of stable transfectants. Using standard amount of lipofectamin (10µl/well) in 6-well plate, highest expression level of green fluorescent protein (GFP) was shown after transfection of CHO-K1 cells with 4µg/well pEGFP-c1 while highest expression level of rhEPO was observed after transfection of CHO-K1 cells with 6, 8, and 10µg/well pJ-EPO plasmids. The data also indicated that optimal transfection conditions of CHO-K1 and CHO-S cells with pJ-EPO were shown with the use of 4µg/well DNA and 15µl lipofectamin, respectively. Concentration of G418 affected the expression where strongest rhEPO expression was shown at 1,000 ng/µl G418 concentration.

Guide for Authors AB Vol 15 No 2 (2011) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Back Cover AB Vol 21 No 2 (2017) Syamsidah Rahmawati
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Association between Polymorphism of β-Lactoglobulin Gene on Milk Yield and Quality in Local Sheep at Jonggol Animal Science Teaching and Research Unit (JASTRU) Cece Sumantri; Nurhayati D; Ahmad Farajallah; A Anggraeni
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The aims of this study were to identify the effects polymorphism of β-lactoglobulin gene on milk yield and percentage of milk protein and fat in local sheep. A total number of 83 heads of lactating ewes raised under an extensive management at Jonggol Animal Science Teaching and Research Unit (JASTRU) of the Faculty of Anima Science, Bogor Agricultural University were studied. Research activities were carried out through some step involving blood collection. DNA isolation, DNA amplification and separation of DNA fragments by electrophoresis with silver staining method. By using polymerase chain reaction (P R) then genotyped by single strand conformation polymorphism technique (SSCP), it was success fully amplified a fragment length at 420 bp in β-lactoglobulin gene located on the exon 7. The electrophoresis pattern revealed 5 types of β-lactoglobulin gene and designated as AB. C, D and E types. The proportion for those respective 5 type from the highest to the lowest were for A (27.7%)C (16.87%), D (12.05%), (10.84%) and B (9.64%) respectively. There were no significant effect polymorphism β-lactoglobulin gene on both milk yield and quality in I cal sheep at JASTRU. Keywords : polymorphism. β-lactoglobulin in gene, PCR-SSCP and local sheep

Production of Phytase, Amylase and Cellulase by Aspergillus niger, Neurospora crassa, and Rhizophus oryzae on Sargassum and Rice Bran Under Solid State Fermentation Atit Kanti
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The objective of study was to produce amylase, cellulase and phytase on sargassum and rice bran on solid state fermentation using Aspergillus niger, Neurospora crassa and Rhizophus oryzae. Media for solid state fermentations composed of dried sargassum and rice bran. The effect of particle of sargassum, initial moisture content on phytase, amilase, and cellulase were evaluated. Optimum enzyme activity of phytase, amylase and cellulase were obtained after 4 days fermentation at 30°C, and initial moisture content was adjusted to 60%. The optimum particle size of dried sargassum attaining the highest enzyme activity was 25 mesh. Best formula forenzymes production was at the ratio of 4:6 (w/w) of Sargassum spinosum (SS) and rice bran (RB) respectively. At this formula highest phytase activity was obtained by Aspergillus niger, cellulase by Rhizopus oryzae, and amylase by Neurospora crassa. Media composed of sargassum and rice bran can be used for phytase, amylase and cellulase production.

Agrobacterium-mediated transformation of banana Musa acuminata AA cv "“Mas Lampung” with hpt gene using sterile corm as target tissue Ade Nena; Witjaksono Witjaksono; Amy Estiati
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The protocol for Agrobacterium-mediated transformation of local banana plants cv “Mas Lampung” (AA) has been established. A selectable marker gene (hpt) has been used to study the transformation using in vitro corm slices as target tissues. Banana in vitro corm slices were co-cultivated with the EHA105 strain of Agrobacterium tumefaciens harbouring binary vector pCAMBIA 1301 containing hygromycin resistance gene (hpt) as a selectable marker and intron-containing β-Glucuronidase (gus-intron) gene as a reporter gene driven by CaMV 35S promoter. Polymerase Chain Reaction (PCR) were used to examine the existence of hpt gene in plants resulted from the transformation. Using primer pairs specific for hpt gene, our PCR analysis on leaves showed the presence of the hpt transgene in banana transgenic plants at first generation (T0) of transformation. To prove the existence of hpt gene in the fruits of transgenic banana plants, PCR analysis were also carried out. The data showed that the hpt gene could be amplified from banana fruits of tested samples. These result demonstrates that the Agrobacterium-mediated transformation method used in this experiment has been successful to transfer gene into banana plants. Thus, the transformation method reported here could be used as a standard protocol to transfer another useful genes into local banana plants cv. “Mas Lampung”. Furthermore, the presence of transgene in fruits of banana transgenic plants is important achievement especially for transgene that is expected to be expressed in the fruit including to introduce vaccine genes into banana fruits for edible vaccine.Key words: Agrobacterium, hpt gene, transgenic banana Mas Lampung, Musa acuminate corm slices, PCR

Editor's Preface AB Vol 18 No 1 (2014) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Front Cover AB Vol 15 No 1 (2011) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2011.v15.n1.%p

Page 32 of 54 | Total Record : 540

30 31 32 33 34

Filter by Year

2004 2021

Filter By Issues

All Issue Vol 25, No 1 (2021): ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses Vol 24, No 1 (2020): Annales Bogorienses Vol 23, No 2 (2019): Annales Bogorienses Vol 23, No 1 (2019): Annales Bogorienses Vol 22, No 2 (2018): Annales Bogorienses Vol 22, No 2 (2018): Annales Bogorienses Vol 22, No 1 (2018): Annales Bogorienses Vol 22, No 1 (2018): Annales Bogorienses Vol 21, No 2 (2017): Annales Bogorienses Vol 21, No 2 (2017): Annales Bogorienses Vol 21, No 1 (2017): Annales Bogorienses Vol 21, No 1 (2017): Annales Bogorienses Vol 20, No 2 (2016): Annales Bogorienses Vol 20, No 2 (2016): Annales Bogorienses Vol 20, No 1 (2016): Annales Bogorienses Vol 20, No 1 (2016): Annales Bogorienses Vol 19, No 2 (2015): Annales Bogorienses Vol 19, No 2 (2015): Annales Bogorienses Vol 19, No 1 (2015): Annales Bogorienses Vol 19, No 1 (2015): Annales Bogorienses Vol 18, No 2 (2014): Annales Bogorienses Vol 18, No 2 (2014): Annales Bogorienses Vol 18, No 1 (2014): Annales Bogorienses Vol 18, No 1 (2014): Annales Bogorienses Vol 17, No 2 (2013): Annales Bogorienses Vol 17, No 2 (2013): Annales Bogorienses Vol 17, No 1 (2013): Annales Bogorienses Vol 17, No 1 (2013): Annales Bogorienses Vol 16, No 2 (2012): Annales Bogorienses Vol 16, No 2 (2012): Annales Bogorienses Vol 16, No 1 (2012): Annales Bogorienses Vol 16, No 1 (2012): Annales Bogorienses Vol 15, No 2 (2011): Annales Bogorienses Vol 15, No 2 (2011): Annales Bogorienses Vol 15, No 1 (2011): Annales Bogorienses Vol 15, No 1 (2011): Annales Bogorienses Vol 14, No 2 (2010): Annales Bogorienses Vol 14, No 2 (2010): Annales Bogorienses Vol 14, No 1 (2010): Annales Bogorienses Vol 14, No 1 (2010): Annales Bogorienses Vol 13, No 1 (2009): Annales Bogorienses Vol 13, No 1 (2009): Annales Bogorienses Vol 12, No 1 (2008): Annales Bogorienses Vol 12, No 1 (2008): Annales Bogorienses Vol 11, No 1 (2007): Annales Bogorienses Vol 11, No 1 (2007): Annales Bogorienses Vol 10, No 1 (2005): Annales Bogorienses Vol 10, No 1 (2005): Annales Bogorienses Vol 9, No 2 (2004): Annales Bogorienses Vol 9, No 2 (2004): Annales Bogorienses Vol 9, No 1 (2004): Annales Bogorienses Vol 9, No 1 (2004): Annales Bogorienses More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota adm. jakarta selatan,

Dki jakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota adm. jakarta selatan, Dki jakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota adm. jakarta selatan,

Dki jakarta

INDONESIA