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ANNALES BOGORIENSES

annales
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 05178452 EISSN : 24077518 DOI : -

Core Subject : Health, Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.

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Articles 540 Documents

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Guide for Authors AB Vol 16 No 1 (2012) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Guide for Authors AB Vol 23 No 2 (2019) syamsidah rahmawati
ANNALES BOGORIENSES Vol 23, No 2 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Back Cover AB Vol 12 No 1 (2008) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2008.v12.n1.%p

Back Cover AB Vol 22 No 1 (2018) Syamsidah Rahmawati
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Potency of Endophyte Bacterium Isolated from Shorea selanica on Producing IAA Hormone and Supporting the Growth of Soybean Tiwit Widowati; Nuriyanah Nuriyanah; Harmastini Sukiman
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Growth of soybean plants was supported by the presence of nitrogen fixing bacteria. Besides nitrogen, other elements such as phosphate, potassium and growth hormones are also required. Endophytic bacteria associated with Shorea selanica were isolated and tested for their ability to produce indole acetic acid (IAA) hormone and exhibit stress tolerance. Colorimetric analysis showed that isolate SSBt2 produced the highest IAA (43.01 µg/ml) in culture supplemented with L-tryptophan. Isolate SSBt2 grew well in the some stress tests, except on heat and oxidative stress. The highest IAA producing strain was selected for determining its capability and compatibility to support the growth of soybean plants in glass house experiment. The results indicated that the endophytic bacteria isolated from S. selanica are compatible to support the growth of soybean. SSBt2 was identified as Enterobacter hormaechei based on 16S rRNA gene analysis.

Back Cover AB Vol 19 No 2 (2015) puspita lisdiyanti
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2015.v19.n2.%p

Segregation Analysis of Transgenic Rice Plants CV Rojolele Harboring Cry1B Gene and Plant Selection for Potential to Yellow Stem Borer Amy Estiati; Yuli Sulistyowati; Fatimah Zahra; Ade Nena Nurhasanah; Syamsidah Rahmawati; Inez H S-Loedin
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Transgenic rice plants harboring resistant gene to yellow stem borer, cry1B gene had been obtained. However, the cry1B gene in second generation of transgenic rice lines still segregating following Mendelian ratio 3:1. For further use in the breeding programs, it is important to ensure that the gene is dominant gene and the plants are homozygous for cry1B gene. Selection of homozygous transgenic rice lines containing cry1B gene at third, fourth and fifth generation had been conducted by PCR. The presence of cry1B gene was determined by showing the PCR product of 1.9 kb. Segregation analysis proved that six transgenic rice lines i.e. 3R7.8.15.1, 3R7.8.15.9, 3R7.8.15.10, 3R7.8.15.15, 3R7.8.15.21, 3R7.8.15.29 are homozygous lines for cry1B gene. Moreover, bioassay studies at vegetative stage on six homozygous transgenic rice lines showed that these transgenic rice lines are potential resistant to yellow stem borer comparing with non-transgenic plants, with the score of 0 (indicated no symptom) for six transgenic lines and score of9 (more than 60% damaged tillers ) for non-transgenic plants. However, to confirm the efficacy of cry 1B gene to yellow stem borer in naturalcondition, confined field trial in endemic area of yellow stem borer should be conducted. Keywords: bioassay, cry1B gene, transgenic rice, yellow stem borer

In-Silico Cloning and Analysis of Divalent Subunit OMP31-SODc Proteins As A Prophylaxis Vaccine Against Brucella melitensis Infection Sri Kartika Wijaya; Arizah Kusumawati; Andri Wardiana; Yana Rubiana; Ulfatul Husnaa; Adi Santoso
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The urgency to develop a new protein based subunit vaccine candidate against Brucella was provoked by its frequent infection to human and lives stock. Since Brucella melitensis is found as the most species isolated from human, thus the outer membrane of the Brucella melitensis become prominent subcellular location for searching promising antigen to be developed as vaccine target due to its interaction with cell host. Among other proteins suggested by Vaxign program, the OMP31 is found as a promising candidate. Moreover, analysis on other subcellular location leads our interest to SODc protein, which is expected to support the OMP31 in triggering immune response. The OMP31-SODc divalent vaccine candidate was analysed in-silico to predict its stable three-dimensional structure, cloning process and expectation on the ease during expression, purification and the protein vaccine delivery to develop expected immune response.

Modification of Plasmids and Cry Genes of Bacillus Thuringiensis subsp. kurstaki Lid-I After Treatment With Ethymethanosulfonate (EMS) and UV Light Eddy Jusuf
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Bacillus thllringiensis subsp. kllrstaki HD-J is a potential insecticidal bacterium producing five type of a -endotoxin crystal proteins. This bacterium is widely commercialized due to its wide spectrum toxicity against both Lepidopteran and Dipteran larvae . The objective of this work was to create autolysin deficient mutant causing cell fails to lyse. This mutant yield intact cell s within spore and a -endotoxin crystal protein protected inside, from which an undamaged active bio-insecticide wou ld be obtained . Two methods of mutagenesis, 2ry( of ethylmethanosulfonate and 10, 25, and 50 second of UV light expo ure, resu lted in mor loss of motility) mutation. Observation showed that 14 of 20 survived mutants have lost some of its plasmids (varied from one to five). wh ile the other six maintained their plasmids. By employing the polymerase chain reaction (PCR) the change on the cry genes was studied.

Editorial Boards AB Vol 10 No 1 (2005) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

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