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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Ekayanti Mulyawati Kaiin; Wahono Esthi Prasetyaningtyas; Kusdiantoro Mohamad; Ita Djuwita; Tuty Laswardi Yusuf; Mohamad Agus Setiadi
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.602 KB) | DOI: 10.14203/ann.bogor.2018.v22.n1.20-26

Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.  LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%  LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with  LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM  increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
Chemical Compositions and Antibacterial Effect of Essential Oil of Key Lime Leaves (Citrus Aurantifolia Swingle: Rutaceae) Yuliasri Jamal; Aris Medta Pariwidjayanti; Andria Agusta
ANNALES BOGORIENSES Vol 16, No 2 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3848.842 KB) | DOI: 10.14203/ann.bogor.2012.v16.n2.23-28

Abstract

Steam distillation of essential oil from fresh leaves of Key Lime (Citrus aurantifolia Swingle) gave 0.4% yellow essential oil with distinctive fragrance. Five out of 40 components of the essential oil were identified as the major components with content above 5%, they were geranial (10.3%), limonene (10.2%), neral (8.94%), caryophyllene (5.72%), and citronellal (5.41%). The essential oil was active against four bacterial test, Bacillus subtilis NBRC 3134, Staphylococcus aureus NBRC 14276, Micrococcus luteus NBRC 14218, and Escherecia coli NBRC 14237. B. Subtilis was the most sensitive bacterium with the widest inhibition area at 50% concentration of essential oil. Minimum Inhibition Concentration (MIC) value of essential oil against B. Subtilis was 0.125%.
The Emergence of Biosimilars in Indonesia: Guidelines, Challenges and Prospects Andri Wardiana; Ratih Asmana Ningrum
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (259.258 KB) | DOI: 10.14203/ann.bogor.2016.v20.n2.37-46

Abstract

According to the Food and Drug Administration (FDA), biosimilar is defined as a product which is highly similar to the reference product without clinically meaningful differences in safety, purity and potency. Indonesia is a developing country which has more than 250 million people. The domestic need of Biosmilar in Indonesia is about 10-20%.  Even though the need is very high, Indonesia still has not been able to produce Biosimilar independently. To stimulate the domestic production on biosimilar, National Agency for Drug and Food Control (NADFC) Republic of Indonesia has assigned Regulation of Biosimilar as Peraturan Kepala Badan Pengawas Obat Dan Makanan Republik Indonesia Nomor 17 Tahun 2015 Tentang Pedoman Penilaian Produk Biosimilar. The guidance covers the quality requirement and evaluation of Biosimilar products. The Ministry of Health of Indonesia has a strategic plan in biopharmaceutical covering biosimilar which is going to develop in 2015-2025. The strategy is expected to initiate biosimilar production in Indonesia. This review focuses on the guidelines, challenges and prospects biosimilars in Indonesia comparing to other international regulatory bodies.Keywords: Biosimilar, Indonesia, Guidelines, Challenges and Prospects
Gene flow from genetically modified rice to their weedy and wild relatives and its environmental consequences Amy Estiati; Satya Nugroho
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.249 KB) | DOI: 10.14203/ann.bogor.2009.v13.n1.37-45

Abstract

Rice is one of the most important food crops in the world.  Nearly half of the world population consume rice as their staple food (FAO, 2004).  With the increasing of world population, the need to provide more food supplies including rice is obvious.  Biotechnology is expected to play major roles in the improvement of crop productivity and quality. Unlike transgenic maize, eventhough many research have been done to improve rice performance by genetic engineering, transgenic rice has not been released or commercialized.  Among those research to improve rice performance are the atempt to introduce biotic and abiotic stress tolerant traits, herbicide tolerant trait and beta-carotin biosynthetic pathway.  Currently, several genetically modified rice cultivars have been and being tested on limited field trials.Keywords : Rice, stress tolerant
Editor's Preface AB Vol 18 No 2 (2014) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.34 KB) | DOI: 10.14203/ann.bogor.2014.v18.n2.%p

Abstract

Front Cover AB Vol 15 No 2 (2011) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2011.v15.n2.%p

Abstract

Back Cover AB Vol 23 No 1 (2019) ario tutuko
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.108 KB) | DOI: 10.14203/ann.bogor.2019.v23.n1.%p

Abstract

Site-Directed Mutagenesis of Glu-269 L-Arabinose Isomerase from Geobacillus stearothermophilus Isolated from Tanjung Api Poso, Indonesia Dewi Fitriani Fitriani; Puspita Suci Wulandari; Budi Saksono
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.974 KB) | DOI: 10.14203/ann.bogor.2010.v14.n2.33-37

Abstract

Industrializing of tagatose requires enzymes that meet to industrial need such as thermophile, slightly acidic and metal independent.  Previously,  we  cloned,  sequenced  and  expressed  L-arabinose  isomerase  from  Geobacillus stearothermophilus  isolated  from Tanjung  Api, Poso, Indonesia. Based on DNA alignment analysis, the gene had  high  homology  with  those  of  G.  stearothermophilus  T6  (Gene  Bank  Acc  No:  AAD45718)  which  has optimum  activity  at  high  temperature  and  alkaline  condition.  In  this  p aper,  we  described  site-directed mutagenesis approach to mutate Glu-269 (Q269) to Lys-269 (K269) to decrease the optimum pH of the strain. Sequencing  result  showed  that  mutagenesis  had  been  successful  to  mutate  amino  acid  at  position  269  from glutamine (Q) into lysine (K). Expression of mutant Q269 showed protein with molecular mass ~56 kDa. Keywords: site-directed mutagenesis, Q269K, L-arabinose isomerase, Geobacillus stearothermophilus
Back Cover AB Vol 19 No 1 (2015) Muhamad Dzikri
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.521 KB) | DOI: 10.14203/ann.bogor.2015.v19.n1.%p

Abstract

Construction of Binary Vector With Wound Inducible Promoter for Hbsag Expression: Development of Plant-Based Edible Hepatitis B Vaccine from Indonesian Isolate Sony Suhandono; Ernawati A.Giri Rachman; Ima M. Zainuddin; Putri Dwi Utari; Apsari Supraba; Amy Estiati
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3283.881 KB) | DOI: 10.14203/ann.bogor.2007.v11.n1.7-14

Abstract

Hepatitis B is a serious infectious disease In the third world countries including Indonesia . Vaccination is  the most effective way to prevent the spread of the disease;  therefore the demand for HBV vaccine is high.  In order to produce more vaccine at lower cost, transgenic plant can be chosen to express the vaccine with the above criteria. Several researches were successfully producing transgenic plants expressing HBsAg that formed virus-like particles and  induced  immune response  in  human.  However. HBsAg expression  in  transgenic plant needs to be  improved especially on gene  expression control system. Here, we describe the construction of HBsAg .  structural gene under the control of wound  inducible promoter, MeEFl promoter from  manihot esculenta Crantz. The HBsAg gene was amplified using PCR from HBV genome isolated from an  Indonesian patient. The gene was subsequently fused with VSPaS signal peptide, which targeted the reticulum endoplasm of plant cell . The construct was cloned into binary expression vector for Agrobacterium plant Transformation  in  near future.Keywords: HBsAg. VSPaS signal peptide and MeEFI promoter

Page 35 of 54 | Total Record : 540

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