cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
 36   37   38   39   40 
An Application of Reverse Transcriptase Polymerase Chain Reaction in A Relative Quantification of Gene Expression Adi Santoso
ANNALES BOGORIENSES Vol 9, No 1 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4656.928 KB) | DOI: 10.14203/ann.bogor.2004.v9.n1.9-19

Abstract

The ability to quantify steady state levels of individual mes enger-RNA (mRNA) transcripts has been the key issue for study on the control of gene expression. Although two available techniques, Northern blot and nuclease protection assays (NI)A) have been widely used tor detecting mRNA, these techniques have critical limitations. The most obvious limitation of these two techn iques is the required number of target mRNAs to be detected. Reverse transcription-polymerase chain reaction (RT-P R), which has been accepted as a highly sensitive and specific method, provides a means for detecting and quantifying gene expression using, theoretically only a single molecule of mRNA. The ensitivity and reliability ofRT-PCR i dependent upon both the RT and PCR steps. The PCR step ha been problematic because of the exponential nature of this reaction where small variation can lead Lo dramatic changes in final result. here fore, the use of RT-P R for quanti fication of gene expres ion requires pre-experimental planntng and de ign. In thiS experiment, the procedure fOT pre-experimental planning, linear range determination and subsequent relative quantification of gene expression are described in detail. study of ornithine decarboxyJase gene, a gene involved in the polyamine biosynthesis and temporally expressed, dunng embryogenesis of Musca domestica (housefly) was used as the model. The re ults show that during early embryogenesis (t-l to t-4) the expression lev I was very low. The increase In expression profile was observed started at t-5, peaked at t-9, and followed by substantial decrease from t-l 0 to t- 12.  
Front Cover AB Vol 16 No 1 (2012) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2012.v16.n1.%p

Abstract

Pectinase Production and Clarification Treatments of Apple (Malus Domestica) Juice Cocok Ana Maryani Berutu; Fahrurrozi Fahrurrozi; Anja Meryandini
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (351.911 KB) | DOI: 10.14203/ann.bogor.2017.v21.n2.63-68

Abstract

Pectinases are a group of an enzyme that break down pectin, a polysaccharide that is found in plant cell walls. Today, the application of pectinolytic enzymes plays an important role in food technology for the maceration of fruits and vegetables, including for the extraction and clarification of juice. This research aimed to produce pectinase enzyme for clarifying of apple juice. A microbial culture was selected from cocoa bean fermentation samples and identified as Bacillus sp.. The highest enzyme activity was investigated after 48 hours of incubation. Citrus pectin as the carbon source and peptone as the nitrogen source was found as the best component for pectinase production. The optimum condition of pectinase activity was observed at pH 5, temperature 40 °C and the crude enzyme had the higher activity at one hour storage. Apple juice was treated with the enzyme at different concentrations (0%, 0.5%, 1%, 2%, 4%). Apple juice clarification was evaluated for its percent clarity and viscosity. The result showed that enzyme treatment at 4% in apple juice promoted juice clarification and decreased pH and viscosity. In conclusion, the quality of apple juice can be improved by enzymatic treatment using pectinase.
Construction of an EPO (Human-Erythropoietin) Synthetic Gene Through a Recurvise-PCR Method Asrul Muhamad Fuad; Tutus Gusdinar; Debbie Sofie Retnoningrum; Dessy Natalia
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7152.292 KB) | DOI: 10.14203/ann.bogor.2008.v12.n1.25-38

Abstract

Human  erythropoietin (hEPO)  is an  important glycoprotein  in human  that is coded by a single gene named EPO  (eryhtopoietin). EPO  is  a  glycoprotein  hormone  that  promotes  erythropoiesis,  which  is  the  formation process of mature  red  blood  cell  (erythrocytes)  in  human  bodies.  It  is  widely used  for  treatment  of anemia  in patient  With  chronic  renal failure.  Therefore  EPO has  been classified  as hematopoietic cytokine. Recombinant hEPO  (rhEPO)  has  been  commercially  available,  such  a  Epogen.  It  is  produced  in  mammalian  cell, such  as CHO  (Chine  e hamster ovary) cells  for  the  reason of  its complex structure as a glyco-protein. In  an effort  to  use and optimize heterolgous EPO gene expression  in  an  alternative eukaryotic  host  cells  such  as  yeast, an  EPO­synthetic  gene  (EPOsyn)  was  constructed.  The  synthetic  gene  had  been designed to contain  optimaI Pichiapastoris codon usage . It had  been constructed by a  recursive-PCR method  in  two-step PCR reactions. The gene was assembled  from  8 single strands synthetic  oligonuclotides having an average  length of 90 nt with 20  to 30 overlap  region  between  two  adjacent  oligos. The  synthetic  gene  has  less  GC  content  (4-.3 1%)  compared  its native (human) gene (59.08%). The synthetic gene has  been cloned  in  pCR2.1  cloning plasmid and sequenced. From  8  independent clones,  it was revealed  that  the error  rate  was  1.59%,  in which  1.42% was due  to deletions and 0.17% due  to substitutions. Design of  the gene sequences, construction method and DNA sequence analysis of  the gene will  be discussed  in  this  paper.   Keywords: Human erythropoietin (hEPO), erythropoiesis  EPO­synthetic  gene, recursive-PCR, Pichiapastoris, hematopoietic cytokine.
Isolation and Identification of Antiplasmodial Compound from Methanol Extract of Calophyllum bicolor P. F. Steven Jamilah Abbas; Muhammad Hanafi; Nina Artanti; Andini Sundowo; Minarti Minarti; Puji Budi Setia Asih; Din Syafrudin
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (742.591 KB) | DOI: 10.14203/ann.bogor.2015.v19.n2.49-56

Abstract

Calophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Central Kalimantan. Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharmaceuticals. The aim of this research was to explore the antiplasmodial activity of methanol extract of Calophyllum bicolor P.F. Steven against Plasmodium falciparum. The methanol extract was purified by colomn chromatography system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1H-&13C-NMR and 2D-NMR, [COSY, HMBC and HMQC] data and the isolated compound was identified as xanthone. Methanol extract showed antiplasmodial activity growth inhibition against P. falciparum with IC50 5.2 ppm and the new 5-methoxy trapezifolixanthone compound have maximum inhibition at concentration 0.11 nMol.
Propagation of Sukun (Artocarpus altilis (Parkinson) Fosberg) through In Vitro Shoot Proliferation Maria Imelda; Aida Wulansari; Laela Sari
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.265 KB) | DOI: 10.14203/ann.bogor.2009.v13.n1.21-28

Abstract

Sukun (Artocarpus altilis (Parkinson) Fosberg) of the Moraceaae is a big tree which can grow to 15-20 m in height and native to  the Asia-Pasific region.  Beside its delicious fruit, sukun is also known as a  traditional herbal medicinal plant in the region, including Indonesia.  Nearly all parts of the  plant, such as roots, stems and leaves are believed by local communities to be capable of curing liver disease, hypertension, cardiac arrest, toothache, renal problem and even skin itchiness. The collaborative research between LIPI and PR China, on developing herbal medicines indicated that sukun has a great potential for treating cardiovascular disease.  However, the availability of raw materials still poses a big constraint for the industry of herbal medicines. Generally, sukun  is propagated by root or stem cuttings, since in Indonesia  sukun does not produce any seeds. However such  method only produces limited planting materials. In general tissue culture propagated plants have many advantages, namely being clonal,   free from pest and diseases, more uniform,  and  allowing a high rate of  plant multiplication. Therefore, the technique for sukun propagation has been developed by the LIPI Research Centre for Biotechnology. In this research the effects of  1-5 mg/l benzyl amino purine (BAP) and 20-40 mg/l adenine sulphate (AS) on shoot bud proliferation were investigated using lateral shoot buds on a modified Murashige and Skoog (MS) medium with addition of 150 ml/l coconut water(CW). Shoots were rooted on MS medium without plant growth regulators (PGRs). The results showed that the best medium for  in vitro shoot proliferation was a modified MS medium containing 2 mg/l BAP, 40 mg/l AS and 150 ml/l CW. The best medium for rooting is MS medium  containing 1 mg indole butyric acid (IBA), producing roots within 3 weeks. Keywords : Sukun (Artocarpus altilis),  in vitro shoot proliferation, Benzyl amino purine (BAP), Adenine sulphate (AS), coconut water (CW), Indole butyric acid (IBA)
Editorial Boards AB Vol 18 No 1 (2014) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.069 KB) | DOI: 10.14203/ann.bogor.2014.v18.n1.%p

Abstract

Back Cover AB Vol 15 No 1 (2011) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2011.v15.n1.%p

Abstract

Front Cover AB Vol 23 No 1 (2019) ario tutuko
ANNALES BOGORIENSES Vol 23, No 1 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (227.105 KB) | DOI: 10.14203/ann.bogor.2019.v23.n1.%p

Abstract

Editor's Preface Vol 9 No 2 (2004) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.838 KB) | DOI: 10.14203/ann.bogor.2004.v9.n2.%p

Abstract

Page 38 of 54 | Total Record : 540

 36   37   38   39   40 

Filter by Year

2004 2021


Reset
Filter By Issues
All Issue Vol 25, No 1 (2021): ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses Vol 24, No 1 (2020): Annales Bogorienses Vol 23, No 2 (2019): Annales Bogorienses Vol 23, No 1 (2019): Annales Bogorienses Vol 22, No 2 (2018): Annales Bogorienses Vol 22, No 2 (2018): Annales Bogorienses Vol 22, No 1 (2018): Annales Bogorienses Vol 22, No 1 (2018): Annales Bogorienses Vol 21, No 2 (2017): Annales Bogorienses Vol 21, No 2 (2017): Annales Bogorienses Vol 21, No 1 (2017): Annales Bogorienses Vol 21, No 1 (2017): Annales Bogorienses Vol 20, No 2 (2016): Annales Bogorienses Vol 20, No 2 (2016): Annales Bogorienses Vol 20, No 1 (2016): Annales Bogorienses Vol 20, No 1 (2016): Annales Bogorienses Vol 19, No 2 (2015): Annales Bogorienses Vol 19, No 2 (2015): Annales Bogorienses Vol 19, No 1 (2015): Annales Bogorienses Vol 19, No 1 (2015): Annales Bogorienses Vol 18, No 2 (2014): Annales Bogorienses Vol 18, No 2 (2014): Annales Bogorienses Vol 18, No 1 (2014): Annales Bogorienses Vol 18, No 1 (2014): Annales Bogorienses Vol 17, No 2 (2013): Annales Bogorienses Vol 17, No 2 (2013): Annales Bogorienses Vol 17, No 1 (2013): Annales Bogorienses Vol 17, No 1 (2013): Annales Bogorienses Vol 16, No 2 (2012): Annales Bogorienses Vol 16, No 2 (2012): Annales Bogorienses Vol 16, No 1 (2012): Annales Bogorienses Vol 16, No 1 (2012): Annales Bogorienses Vol 15, No 2 (2011): Annales Bogorienses Vol 15, No 2 (2011): Annales Bogorienses Vol 15, No 1 (2011): Annales Bogorienses Vol 15, No 1 (2011): Annales Bogorienses Vol 14, No 2 (2010): Annales Bogorienses Vol 14, No 2 (2010): Annales Bogorienses Vol 14, No 1 (2010): Annales Bogorienses Vol 14, No 1 (2010): Annales Bogorienses Vol 13, No 1 (2009): Annales Bogorienses Vol 13, No 1 (2009): Annales Bogorienses Vol 12, No 1 (2008): Annales Bogorienses Vol 12, No 1 (2008): Annales Bogorienses Vol 11, No 1 (2007): Annales Bogorienses Vol 11, No 1 (2007): Annales Bogorienses Vol 10, No 1 (2005): Annales Bogorienses Vol 10, No 1 (2005): Annales Bogorienses Vol 9, No 2 (2004): Annales Bogorienses Vol 9, No 2 (2004): Annales Bogorienses Vol 9, No 1 (2004): Annales Bogorienses Vol 9, No 1 (2004): Annales Bogorienses More Issue