Garuda - Garba Rujukan Digital

All

ANNALES BOGORIENSES

annales
Website

Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : 05178452 EISSN : 24077518 DOI : -

Core Subject : Health, Science, Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology

The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.

Arjuna Subject : -

Articles 540 Documents

38 39 40 41 42

Editorial Boards AB Vol 12 No 1 (2008) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Partial Purification, Characterization, and Application of Extracellular Aspartic Protease from Lactobacillus casei WSP in Producing the Bioactive Peptides with Antibacterial and Antioxidant Activity Akhmad Solikhin; Apon Zaenal Mustopa; Suharsono Suharsono; Wendry Setiyadi Putranto
ANNALES BOGORIENSES Vol 22, No 2 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Lactobacillus casei WSP-derived an aspartic protease was sequentially purified by using chromatography gel filtration sephadex G-50. It resulted in a 22.81-fold increase of specific activity (51.5 U/mg) with a final yield of 1.9%. The estimated molecular weight of the purified enzyme was 37 kDa and showed gelatinolytic activity in zymogram assay. The enzyme exhibited optimum activity at 40ºC and pH 6 with casein as the substrate. Enzyme activity was significantly inhibited by pepstatin A (0.5 mM and 1 mM), confirming that this enzyme is a group of aspartic proteases, while other inhibitors such as EDTA, PMSF and iodoacetic acid showed no inhibition effect on the activity of enzyme. The addition of metal ion to the enzyme decreased enzyme activity, indicating the proteolytic enzyme was metal ion- dependent. Denaturant such as DDT tended to increase caseinolytic activity. Furthermore, this enzyme was capable of generating the new peptides from skimmed milk with the size 8 kDa, 10 kDa and 15 kDa. These peptides have potential as antibacterial and antioxidant agents.

Isolation and Screening of Surfactant-producing Bacteria from Indonesian Marine Environments and Its Application on Bioremediation Dwi Susilaningsih; Fumiyoshi Okazaki; Yopi Yopi; Yantyati Widyastuti; Shigeaki Harayama
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Isolation and screening have been undertaken on oil-degrading microbes from Indonesian marine environments. During screening process it has been found many bacterial isolates capable of degrading crude oil. Hence, study has been focused on the biodiversity of biosurfactant-producing bacterial species in Indonesian marine environment and its function for remedial the pollutant in marine and soil areas. A total of 103 out of 463 isolates showed positive surfactant-degrading properties. By means of partial 16S rRNA gene analyses, it has been found that the majority of taxa are related to Alcanivorax, Pseudomonas, Bacillus, Bortetela, Brucella, Acenitobacter, Staphia, Lysobacter, and Talasosophira. Biosurfactant properties assay showed that they were capable of lowering the surface- and interfacial water tension from 74 mN/m to 40-65 mN/m and from 24 mN/m to 6-10 mN/m, respectively. In addition, most of the surfactants were capable of emulsifying hydrocarbon (crude oil) of 0.01 to 0.15 units, comparable to 0.08 units of synthetic surfactant (20% Tween). Further observation showed that the majority of the surfactants were able to degrade a long chain of alkane, but not branched alkane, with a recovering rate of 20-80%. The application of the surfactant towards oil polluted model beach was done in laboratory scale and showing the surfactant obtained from microbial broth cultures capable for recovering the oil pollutant significantly, compared to the control (without addition microbial broth).

Editorial Boards AB Vol 19 No 2 (2015) puspita lisdiyanti
ANNALES BOGORIENSES Vol 19, No 2 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Screening for Natural Producers Capable of Producing 1,3-Propanediol from Glycerol Dian Andriani; Wien Kusharyoto; Bambang Prasetya; Thomas Wilke; Klaus Dieter Vorlop
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Glycerol is a renewable resource found as the main by-product in the transesterification of triglycerides and fat saponification. Due to the increased production of plant oils, especially palm oil in developing countries, and their larger use by the oleochemical industry, glycerol surpluses are on the world market and this may result in a desrease in glycerol price. As a consequence, biotechnological processes have been developed to convert this substrate into value-added products, such as 1,3-propanediol (1,3-PD). The microbial production of 1,3-PD could be competitive to chemical routes assuming that it is based on cheap raw material and an optimized process. In the screening for 1,3 PD–producing bacteria, raw glycerol as by-product from rapeseed oil processing unit was used as a carbon source compared with commercial glycerol. By using increasing concentration of both glycerols from 50 to 150 g/l, two potential bacteria were obtained from soil samples.BMP 1 was obtained from an enrichment culture using 50 g/l commercial glycerol, while BMR-1 was obtained from an enrichment culture using 100 g/l raw glycerol. The highest conversion yield obtained using the isolateBMP-1 was around 0.62 g 1,3-PD formed per mol glycerol consumed, and 0.73 mol 1,3-PD formed per molgycerol using the isolate BMR-1. No bacteria were obtained from cultures using 150 g/l commercial and rawgycerol, respectively, which indicated that higher concentration of glycerol has inhibition effect. Keywords: 1,3-propanediol, enrichment culture, glycerol, palm oil, screening

Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Nina Artanti; Faiza Maryani; Hani Mulyani; Rizna Triana Dewi; Vienna Saraswati; Tutik Murniasih
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The marine bacteria were cultured in liquid medium under shaking condition were extracted with ethyl acetate. Antidiabetes was measured using inhibition of α-glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method.S creening results showed that at sample concentration of 200 µg/ml, there was significant α-glucosidase inhibitory activity was detected in the extracts of strain sp 7.9 (84% inhibition) and 8.10 (75% inhibition),however the antioxidant activity of these two strains were low only around 30% inhibition, antioxidant activities of other strains were very low.Screening for antibacterial activities using 10µl samples showed that extract of strain Sp 8.5was best for Staphylococcusaureus (14 mm inhibition); Sp 7.9 and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetes and antibacterial.Results of molecular identification conducted by INACC showed that identity of both strain based on BLAST Homology using NCBI database were Bacillus thuringiensis strain Ou2.

Cytological Analysis of Root Cultures of Artemisia Cina Tri Muji Ermayanti; Oktavia Yanti; Erwin Al Hafiizh
ANNALES BOGORIENSES Vol 9, No 2 (2004): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Artemisia cina is a medicinal plant species producing bioactive compounds which are potential as antitumor, antifungal and antibacterial. The aim of this study was to analyze the stabililY of chromosome number in root cultures of A. cina. Transformed root culture was established by infection of leaves of A. cina with Agrobacterium rhizogenes strains 07-20001 . ATCC-15834. A4 and A. tumefaciens strain RlOOO . Roots isolated from glasshouse plants, plantlets grown in solid and liquid MS medium were utilized for investigation of chromosome examination of untransformed roots. Chromosome examination was conducted by squashing method and chromosome numbers were calculated under microscope. The .results showed that both untransformed and transforme root had instability in the chromosome number, but had the modal number of chromosome x=8 with the diploid number of 2n = 4x = 32. Roots isolated from glasshouse plants of A. Cina had 53.7% of cell with the diploid numbers of 2n = 32 and 46.3% of cells had chromosome numbers ranged from 2n = 12 to 2n = 64. Untransformed roots isolated from plantlets cultured in solid medw had only 36.1 % or cells with chromo orne number of 2n = 32, and unlran fomled ro t5 grown in liquid medium had 49.4% of cells with 2n = 32. The chromosome numbers of A. Cina transformed roots was affected by trains of Agrobacterium. Root transformed with the bacterium Strain 07 -20001 showed lhe highest in normal chromosome number of 2n = 32 (62.4%) followed by roots lransformed wiLll strains ATCC-15834 (61.9 %). R1000 (43.6%) and A4 (43.0%). The range of the chromosome number untransformed roots was from 2n= 17 to 2n=64 whilst that of transformed roots was from 2n= 1 l to 20=66

Editor's Preface AB Vol 10 No 1 (2005) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Medium Optimization for Antimicrobial Production By Newly Screened Lactic Acid Bacteria Rohmatussolihat Rohmatussolihat; Puspita Lisdiyanti; Yopi Yopi; Yantyati Widyastuti; Endang Sukara
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Lactic acid bacteria (LAB) are important for prevention of spoilage and pathogenic bacterial growth in foods due to their ability to generate antimicrobial substances. The objective of this study was to screen LAB for antimicrobial activity and to optimize culture medium for antimicrobial production using Response Surface Methodology (RSM) with Central Composite Design (CCD). Optimization of antimicrobial production of selected LAB was conducted with different combinations of glucose, NaCl, inoculum, and temperature. Our experimental results showed that from 129 LAB isolates, 55 showed significant inhibition against Bacillus subtilis, Escherichia coli, Micrococcus luteus, Staphylococcus aureus, Aspergillus niger, and Candida albicans. No isolates inhibited the growth of Aspergillus flavus. Lactobacillus plantarum LIPI13-2-LAB011 was selected for further study on culture medium optimization to inhibit the growth of C. albicans. From statistical analysis, the production of antimicrobial substances was significantly influenced by temperature, NaCl, and concentration of glucose. Furthermore, the optimum concentrations of glucose, concentration of inoculum, temperature, and NaCl were 1.63 %, 3.03%, 33.74°C, and 3.4%, respectively, with a maximum predicted inhibition index of 1.916, which increased 3.56-fold compared to that obtained in medium before optimization processes. The result was confirmed as when the optimum concentration of nutritions used, the inhibition index increased 3.12-fold.

Growth and Phycocyanin Productitivity of Spirulina fusiformis under Various Light Regimes Tjandra Chrismadha; Rizky Agus Waluya
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

A blue green alga Spirulina fusiformis was grown under various light regimes in a laboratory scale experiment. A 500 watt halogen lamp was employed as the light source, while the light variation of 2,000 lux, 4,000 lux, 6,000 lux, 8,000 lux, and 10,000 lux was obtained by placing a series of 2 L experimental bottles at various distance. The growth medium used was modified Zarrouk medium with initial pH of 8.72, and room temperature was 28-30oC. After inoculation the alga was let to grow for 30 days, and observation on the biomass, chlorophyll, crude protein, and phycocyanin content were carried out every 10 days. The algal biomass was determined gravimetrically, the chlorophyll, phycocyanin, and protein content were measured using spectrophotometer after extraction in 90% aceton, pH 7.0 buffered water, and folin-phenol dye binding, respectively. The result shows a remarkable effect of light intensity to the algal biomass as well as the biochemical content. The specific growth rate increased from 0.08 doubling/day at light intensity 2,000 lux to 0.14 doubling/dat at 10,000 lux, which was equivalent to an increase in the biomass productivity of more than 3 times. The highest algal chlorophyll content was observed at light intensity between 4,000-8,000 lux, indicating the optimum light condition at that irradiance range. The protein content was consistently lower with light intensity, from 42.96-52.91% DW at 2,000 lux to 33.71-41.08 % at 10,000 lux. A consistent drop in the protein content also observed with the culture growth phase, from 39.82-52.91% DW in the early growth stage down to 33.71-42.96% at day 30. Light intensity in concomitance with the growth phase remarkably increase the algal phycocyanin content. In the early growth stage the phycocyanin content was ranged from 0.16% DW at 2,000 lux up to 1.229% DW at 10,000 lux, whereas at the end of the experiment the algal phycocyanin content were 1.04% DW and 2.436% DW, at 2,000 lux and 10,000 lux, respectively. It gave a consequence of more than 7 times higher phycocyanin productivity, which was from 0.09 mg/L/day at 2,000 lux to 0.62 mg/L/day at 10,000 lux. This result shows the importance of light factor in producing phycocyanin from the blue green alga Spirulina fusiformis.

Page 40 of 54 | Total Record : 540

38 39 40 41 42

Filter by Year

2004 2021

Filter By Issues

All Issue Vol 25, No 1 (2021): ANNALES BOGORIENSES Vol 24, No 2 (2020): Annales Bogorienses Vol 24, No 1 (2020): Annales Bogorienses Vol 23, No 2 (2019): Annales Bogorienses Vol 23, No 1 (2019): Annales Bogorienses Vol 22, No 2 (2018): Annales Bogorienses Vol 22, No 2 (2018): Annales Bogorienses Vol 22, No 1 (2018): Annales Bogorienses Vol 22, No 1 (2018): Annales Bogorienses Vol 21, No 2 (2017): Annales Bogorienses Vol 21, No 2 (2017): Annales Bogorienses Vol 21, No 1 (2017): Annales Bogorienses Vol 21, No 1 (2017): Annales Bogorienses Vol 20, No 2 (2016): Annales Bogorienses Vol 20, No 2 (2016): Annales Bogorienses Vol 20, No 1 (2016): Annales Bogorienses Vol 20, No 1 (2016): Annales Bogorienses Vol 19, No 2 (2015): Annales Bogorienses Vol 19, No 2 (2015): Annales Bogorienses Vol 19, No 1 (2015): Annales Bogorienses Vol 19, No 1 (2015): Annales Bogorienses Vol 18, No 2 (2014): Annales Bogorienses Vol 18, No 2 (2014): Annales Bogorienses Vol 18, No 1 (2014): Annales Bogorienses Vol 18, No 1 (2014): Annales Bogorienses Vol 17, No 2 (2013): Annales Bogorienses Vol 17, No 2 (2013): Annales Bogorienses Vol 17, No 1 (2013): Annales Bogorienses Vol 17, No 1 (2013): Annales Bogorienses Vol 16, No 2 (2012): Annales Bogorienses Vol 16, No 2 (2012): Annales Bogorienses Vol 16, No 1 (2012): Annales Bogorienses Vol 16, No 1 (2012): Annales Bogorienses Vol 15, No 2 (2011): Annales Bogorienses Vol 15, No 2 (2011): Annales Bogorienses Vol 15, No 1 (2011): Annales Bogorienses Vol 15, No 1 (2011): Annales Bogorienses Vol 14, No 2 (2010): Annales Bogorienses Vol 14, No 2 (2010): Annales Bogorienses Vol 14, No 1 (2010): Annales Bogorienses Vol 14, No 1 (2010): Annales Bogorienses Vol 13, No 1 (2009): Annales Bogorienses Vol 13, No 1 (2009): Annales Bogorienses Vol 12, No 1 (2008): Annales Bogorienses Vol 12, No 1 (2008): Annales Bogorienses Vol 11, No 1 (2007): Annales Bogorienses Vol 11, No 1 (2007): Annales Bogorienses Vol 10, No 1 (2005): Annales Bogorienses Vol 10, No 1 (2005): Annales Bogorienses Vol 9, No 2 (2004): Annales Bogorienses Vol 9, No 2 (2004): Annales Bogorienses Vol 9, No 1 (2004): Annales Bogorienses Vol 9, No 1 (2004): Annales Bogorienses More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota adm. jakarta selatan,

Dki jakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name -

Contact Email -

Phone -

Journal Mail Official -

Editorial Address -

Location Kota adm. jakarta selatan, Dki jakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
-

Contact Email
-

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota adm. jakarta selatan,

Dki jakarta

INDONESIA