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INDONESIA
ANNALES BOGORIENSES
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 05178452     EISSN : 24077518     DOI : -
Core Subject : Health, Science, Agriculture, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Immunology & microbiology Medicine & Pharmacology
The Annales Bogorienses (ISSN: 0517-8452, E-ISSN: 2407-7518) is a peer-reviewed Journal that is published biannually. First published in 1955, it is now one of the oldest scientific journal in the nation. The Annales Bogorienses publishes original articles in basic and applied research as well as critical reviews and short communication in the fields of life sciences with the emphasis in biotechnology, molecular biology, and biochemistry.
Arjuna Subject : -
Articles 540 Documents
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Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity Wendry Setiyadi Putranto; Kusmajadi Suradi; Hartati Chairunnisa; Apon Zaenal Mustopa; Puspo Edi Giriwono; Harsi Dewantari Kusumaningrum; Maggy Thenawidjaja Suhartono
ANNALES BOGORIENSES Vol 21, No 1 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.913 KB) | DOI: 10.14203/ann.bogor.2017.v21.n1.9-14

Abstract

The Lactic Acid Bacteria with Milk Clotting Activity (MCA) were isolated from Bakasam, an Indonesian traditional fermented meat. The isolate screening was carried out using modified method of Skim Milk Agar and Milk Clotting Activity Test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/ml. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% (FR3-F primer) and 99% (FR3-R primer) identic with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.  
The Influence of Harvesting Period on Lipid Associated Antioxidant Activity of Semicontinuously Grown Chlorella vulgaris Tjandara Chrismadha; Diani Sartika; Iriani Setyaningsih; Uju Uju
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.466 KB) | DOI: 10.14203/ann.bogor.2010.v14.n1.25-30

Abstract

A green alga,  Chlorella vulgaris  was grown semi-continuosly at various harvesting periods, and the lipid content and its associated antioxidant activity was examined. The harvesting periods were 9, 18, and 27 days, while the algal culture was placed  in 10 L glass  bottles provided with aeration  for gas exchange and  mixing. Light  was  obtained  from  440  watt  cool  fluorescent  lamps  placed  at  one  side  of  the  culture.  Room temperature was  26-32C.  Algal  lipid  extraction  was  done  based  on  liquid  phase  separation  of  methanol :chloroform:water  and  the  antioxidant  activity  was  examined  by  means  of  oxidation  inhibitory  in ethanol emulsified  limnoleic  acid.  In  addition,  a  TLC  analysis  was  performed  to  identify  the  antioxidant compouns soluble  in  the  lipid.  The  results  showed  that  harvesting  per iod  had  a  significant  influence  on  the algal lipid  content,  which  were  11.94,  12.96,  and  16.51%  of  the  dry  weight  in  the  culture  with  harvesting periods of 9, 18, and 27 days, respectively. No remarkable effect of the  harvesting period on the  antioxidant activity, which were observed to inhibit oxidation of linoleic acid up to 67-71%. There were five compounds found can  be associated   with  the  algal  antioxidant  activity,  which  were  pheophorbide -a,  chlorophyll-b,chlorophyll-a, phaeophytin-a, -carotene, and an unidentified one. Key words: microalgae, Chlorella vulgaris, lipid, antioxidant, harvesting period 
Front Cover AB Vol 20 No 1 (2016) Muhamad Dzikri Anugerah
ANNALES BOGORIENSES Vol 20, No 1 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2016.v20.n1.%p

Abstract

Field Evaluation Of Conde Progenies With Early Heading Date And Resistance To Bacterial Leaf Blight Fatimah Fatimah; Joko Prasetiyono; Ahmad Dadang; Tasliah Tasliah; Masumah Masumah
ANNALES BOGORIENSES Vol 23, No 2 (2019): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.845 KB) | DOI: 10.14203/ann.bogor.2019.v23.n2.87-96

Abstract

Heading date is one of key traits in rice adaptation to different environments. Rice with early maturity is very useful for increasing rice production extensively especially in Indonesia. The improvement of rice variety Conde for heading date and resistance to bacterial leaf blight (BLB) was developed through Marker Assisted Backcrossing approach (MAB). Xa7 derived from Conde as recipient parents for resistance to BLB and Hd2 gene derived from Nipponbare as a donor parent for heading date. The aim of this research was to evaluate the performance of heading date and agronomic traits of 85 BC2F4 lines in two field stations, Sukamandi (West Java) and Maros (South Sulawesi). Molecular analysis of Xa7 on chromosome 6 and Hd2 gene on chromosome 7 and agronomic performance was evaluated. Foreground analysis resulted 41% for Xa7 and 66.7% for Hd2 gene in homozygous condition. The majority of lines have similar heading date and yield with Conde and more resistance to bacterial leaf blight (BLB) than Conde. Six lines flowered earlier 5 days than Conde in Sukamandi and three lines flowered earlier 3 days in Maros. Twelve lines with flowered earlier than Conde, resistance to BLB and similar yield with Conde were selected for background analysis resulted 93% genome recovery. These lines would be further evaluated in preliminary yield trail for their stability.
Editorial Boards AB Vol 11 No 1 (2007) Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.21 KB) | DOI: 10.14203/ann.bogor.2007.v11.n1.%p

Abstract

Front Cover AB Vol 22 No 1 (2018) Syamsidah Rahmawati
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.942 KB) | DOI: 10.14203/ann.bogor.2018.v22.n1.%p

Abstract

Polyaromatic Hydrocarbon Degradation and Dioxygenase Gene Detection from Alteromonas alvinellae Bt05 Ahmad Thontowi; Nanik Rahmani; Yopi Yopi
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15718.693 KB) | DOI: 10.14203/ann.bogor.2013.v17.n1.33-42

Abstract

Bt05 is marine bacterium which was isolated from the Jakarta Bay, Indonesia. The aim of this study was to characterize PAHs-degrading property, molecular identification by partial analysis of 16S rRNA gene and to partially analyze dioxygenase gene of Bt05 isolate. Our further study on this isolate revealed that it could degrade three PAHs (phenanthrene, dibenzothiophene, fluorene) between 60%–90% within 11 days at 100 ppm level. This finding indicated the potential of the isolate for bioremediation of PAHs. The isolate was identified as Alteromonas alvinellae by phylogenetic analysis of 16S rRNA gene sequence. Sequence analysis of the PCR product of PAH dioxygenase genes amplified using two primer set (iiDA and ppAH) of the isolate were identified 97% as naphthalene dioxygenase gene (phaAc) and 58% as 1,2-dioxygenase.
Back Cover AB Vol 20 No 2 (2016) Muhamad Dzikri
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2016.v20.n2.%p

Abstract

Tissue Culture Propagation of “Hausa potato” (Solenostemon rotundifolius (Poir) JK Morton) Witjaksono Witjaksono; Aryani Leksonowati
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.973 KB) | DOI: 10.14203/ann.bogor.2012.v16.n1.1-8

Abstract

We have developed an effective plant propagation method of “hausa potato‟ through shoot proliferation  in vitro, acclimatization in the greenhouse and transplantation of the plantlet on the individual media container for planting material production. The shoot cultures were initiated from shoots that grew from tuber collected from the field, disinfested using standard method and transferred to MS medium with 1 mg/l  of  BA for shoot proliferation. The proliferating shoots were then subcultured to MS medium with no growth regulators to induce rooting and enlarge the shoot. The plantlets were acclimatized in a plastic box containing medium of a mixture of cocopeat, sands, rice husk charcoal and top soil covered with plastic film to maintain humidity. The plantlets survived at 85% and almost 100% grew into planting materials ready for transplanting in the soil.  The planting materials survived in the field and produced tuber normally. Keywords: Solanostemon rotundifolius, hausa potato, kentang hitam, in vitro propagation, acclimatization, planting materials.
Optimization Expression and Stability Test of Recombinant Human Interferon Alfa 2a Fusion Protein in Escherichia coli BL21 (DE3) Adi Santoso; Arizah Kusumawati
ANNALES BOGORIENSES Vol 19, No 1 (2015): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.849 KB) | DOI: 10.14203/ann.bogor.2015.v19.n1.1-8

Abstract

The rhIFN α2a is expressed as a fusion protein containing thioredoxine and polyhistidine sites at its N terminal. Our previous research has obtained recombinant human IFN α2a (rhIFN α2a) protein that expressed predominantly as a soluble form in E. coli BL21(DE3). Through systematic approach of various culture conditions, the aim of current this research is to acquire the best condition and its stability of recombinant rhIFN α2a fusion protein in a culture under study. Expression optimization performed by using three parameters, i.e.: temperature, induction time and inducer concentration. Various IPTG concentrations are 0.25 mM, 0.5 mM, 0.75 mM and 1.0 mM. The incubation time of bacterial cell culture carried out in 3, 4, and 5 hours at temperature 28, 30, and 37°C. The best condition was used to analyze the stability of rhIFN α2a protein expression up to ten generation. The expressed protein was analyzed using SDS PAGE and CBB staining. The optimal culture condition was found to be 37°C temperature with 4 hours time of induction and 1 mM IPTG concentration. Stability analysis revealed that the rhFN α2a protein expression remained stable until the tenth generation with molecular weight, approximately, 36 kDa.

Page 37 of 54 | Total Record : 540

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