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INDONESIA
Jurnal Perikanan Universitas Gadjah Mada
Published by Universitas Gadjah Mada
ISSN : 08536384     EISSN : 25025066     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Jurnal Perikanan Universitas Gadjah Mada are published to promote a critical review of the various investigative issues of interest in the field of fisheries between the researchers, academics, students and the general public, as a medium for communication, dissemination, and utilization of wider scientific activities.
Arjuna Subject : -
Articles 20 Documents
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Search results for , issue "Vol 9, No 1 (2007)" : 20 Documents clear
Protein Aeromonas Hydrophila Sebagai Vaksin untuk Pengendalian Mas (Motile Aeromonas Septicemia) pada Jambal Siam (Pangasius hypophthalamus) Olga Olga; Ririen Kartika Rini; Junius Akbar; Alim Isnansetyo; Langkah Sembiring
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.59

Abstract

Objectives of this study were to find out the protein of Aeromonas hydrophila for vaccine, and to evaluate the efficacy of the protein to control Motile Aeromonas Septicemia (MAS) in catfish (Pangasius hypophthalamus). A. hydrophila was isolated from the kidney of diseased catfish. The isolate was cultured, sonicated and sentrifuged into cell-free extract and debris. The protein in the cell-free extract was precipitated with ammonium sulphate, dialized, then fractionated by gel filtration column chromatography on Sephadex G150.The concentration of protein were estimated by spectrophotometer at 280 nm. Fractions obtained from the gel filtration with high concentration of protein were analized by sodium dodocyl sulphate-polyachrylamide gel electrophoresis (SDS-PAGE). The protein bands obtained from SDS-PAGE with molecular weight more than 10 kDa were tested for immunogenicity to mice. Futhermore, the most immunogenic protein was used for fish vaccination. The vaccinated and unvaccinated fishes were chalenged with A. hydrophila.The results indicated that vaccination with the protein significantly increased (P<0.01) the antibody titer either in mice or catfish. Relative Percent Survival of catfish vaccinated with the protein at 5; 7.5; and 10 µg/fish were 61.54%, 80.77% and 76.92%, respectively. The optimum dose of vaccine was 8.33 µg/fish with maximum RPS of 82.05%.
Sintasan dan Pertumbuhan Benih Pasca Larva Beberapa Subspesies Gurami (Osphronemus goramy) Dewi N. Setyowati; Ign. Hardaningsih; Susilo B. Priyono
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.75

Abstract

The objective of this research was to know the survival and growth of post-larvae of several giant gouramy (Osphronemus goramy) subspecies. Experiment in this study used Completely Randomized Design (CRD). The treatment consisted of three strains of giant gouramy, bastar, bluesafir, and bule. Giant gouramy post-larvae were reared for 45 days. The results showed that there were no significantly different among those subspesies of giant gouramy (P>0,05) on survival rate (bastar=86,5%; bluesafir=61,5%; bule=87,0%), weight and length (bastar=0.946 g, 2.975 cm; bluesafir=0.585 g, 2.860 cm; bule=0.826 g, 3.280 cm), also on the specific growth rate of length (bastar=3,070%/day, bluesafir=2,891%/day, bule=3,308%/day). However there was significantly different on specific growth rate of weight (P<0,05) (bastar=7,356%/day, bluesafir=9,464%/day; bule=11,167%/day).
Pengaruh L-Karnitin terhadap Kadar Lemak Daging dan Komposisi Tubuh Ikan Patin (Pangasius hypopthalmus) Suwarsito Suwarsito
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.65

Abstract

This study was conducted to investigate the effect of dietary L-carnitine level on muscular lipid and body composition of asian catfish (Pangasius hypopthalmus). Four isoprotein and isocaloric diets with different levels of L-carnitine, 0.00% (diet K), 0.10% (diet A), 0.18% (diet B), and 0.29% (diet C) were applied in this experiment. Ten fishes with initial body weight of 35.86 ± 1.54 g were cultivated in each aquarium (water volume 60 l) for 50 days. Fishes were fed three times daily at satiation. The results showed that L-carnitine in the liver increased by increasing dietary L-carnitine level. While increasing dietary L-carnitine until 0.18% decreased content of lipid in the liver, but excessive dose of dietary L-carnitine increased content of lipid in the liver. It was further observed that L-carnitine in the diets reduced the muscular lipid content from 32.35 to 7.51% dry weight, but had no effect on body composition.
Studi Penurunan Glukosa Darah Diabet dengan Konsumsi Rumput Laut Eucheuma cottonii Hardoko Hardoko
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.70

Abstract

The purpose of this study was to observe the effect of Eucheuma cottonii consumption to the diabetic blood glucose level. Study on reducing blood glucose by consuming seaweed E. cottonii was conducted using male wistar rat (Ratus norvegicus). Rats were made to a hyperglycemic condition by aloxan injection before given ransom contains of 5, 10, 15, 20% (w/w) E. cottonii, standard ransom (negative control), and parental glibenklamid (positive control). The result showed that standard ransom was not able to reduce blood glucose from hyperglycemic to normal level, while ransoms with E. cottonii were able to do so. The higher E. cottonii seaweed level in the ransom had the higher capacity to decrease blood glucose level. The ransom with 20 and 15% E. cottonii were able to reduce blood glucose in 18 and 21 days, respectively.This treatment has the same ability as glibenklamid medicine which also reduced blood glucose to normal level in 18 days. Ransom with 5 and 10% E. cottonii were also able to reduce blood glucose level but the normal blood glucose level could not be achieved until 21st day.
STUDI HISTOKIMIA PENYEBARAN LEKTIN MUCUS PADA BERBAGAI JARINGAN TILAPIA (Oreochromis mossambicus) Cahyono Purbomartono; Akihiro Takemura; Kazunori Takano
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.58

Abstract

The aim of this research was to know the distribution pattern of mucus lectin from the different tissue of tilapia (Oreochromis mossambicus). The samples were taken from the epithelium of buccal cavity, gills, esophagus and skin of tilapia with standard body length (SL) 15.2-25.0 cm and body weight (BW) 145-250 g. Histochemical slides were observed under fluorescent microscope. The results showed that tilapia had four kinds of different lectin, such as wheat germ agglutinin (WGA), peanut aglutinin (PNA) and dolichos biflorus (DBA). Wheat germ agglutinin lectin was present in the epithelium of buccal cavity, gill, esophagus and skin. Staining intensity of WGA mucus lectin was strong in the epithelium of buccal cavity, gill, esophagus and skin. While the PNA was present in the epithelium of buccal cavity and esophagus, and DBA lectin was only present in the esophagus. The intensity of staining of PNA was weak, both in the epithelium of buccal cavity and esophagus, same with DBA lectin which was found in the esophagus.
Pertumbuhan, Sintasan dan Keragaan Zoea Sampai Megalopa Rajungan (Portunus pelagicus) Melalui Penurunan Salinitas Bambang Susanto
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.76

Abstract

The purpose of this experiment was to know the influence of lowering salinity to growth, survival rate and performance zoea-megalopa of blue swimming crab. The experiment was done in concrete tank of 3,000 liters water provided with aeration system and initial zoea-1 density of 75 ind/l. Different lowering salinity for larva rearing were A: salinity for zoea-1 to megalopa stages of 32-34 ppt; B: salinity for zoea-1 to zoea-3 stage of 32-34 ppt and zoea-4 to megalopa stage of 28-30 ppt; and C: salinity zoea-1 to zoea-3 stage of 28-30 ppt and zoea-4 to megalopa of 24-26 ppt. The treatment A resulted highest survival rates, and growth rate of megalopa. The survival rate of treatmet A, B, and C were 23.08±4.01%; 17.23±3.41% and 11.54±1.41%, respectively
Studi Kelayakan Lokasi Budidaya Kerapu (Epinephelus sp.) di Perairan Pulau-Pulau Sembilan, Kabupaten Sinjai, Sulawesi Selatan Utojo Utojo; Abdul Mansyur
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.66

Abstract

This study was conducted to find out location which was suitable for culture of grouper (Epinephelus sp.) in Pulau-Pulau Sembilan waters, Sinjai Regency, South Sulawesi. The primary data were collected from each study areas as grouper culture requirement i.e: coastal topography, from the high wave and current, water quality and biological parameters. Secondary data such as wheather, coastal fisheries production, tide probability, Indonesia earth surface map of Sinjai area scale of 1: 75,000, landsat imagery-7ETM digital product and navigation map scale 1: 200,000 were collected before the study. Thematic map of area, suitability as the main expected output of the study was made through spatial analysis and Geographical Information System (GIS). The site suitability for grouper culture in floating net cage was evaluated based on the physical oceanography as limited factors, where factors which influences to the grouper growth and survival were given highest value.The range of values was 0-1. The level of suitability at the value of 1-4, where 4 (100%) = S1 (most suitable), 3 (75-99%) = S2 (suitable), 2(50-74%) = S3 (fair suitable) and 1 (< 50%) = N (less suitable). The potential areas which were suitable for grouper culture in floating net cage development was 303.4 hectares.
Penggunaan Glyroxyl untuk Menghambat Penurunan Mutu Ikan Mas (Cyprinus carpio) Segar Farida Ariyani; Jovita Tri Murtini; Ninoek Indriati; Dwiyitno Dwiyitno; Yusma Yenni
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.71

Abstract

One of the preservative agents that was claimed having bactericidal effect is glyroxyl. This study was aimed to observe the effect of glyroxyl on carp’s (Cyprinus carpio) shelf life. Factorial design was used in this experiment with 9 treatments in triplicates. The fish was immersed in glyroxyl solution at concentrations of 0 (control), 0.1, 0.3 and 0.5% (w/v) for 5 min. Following immersion, fish was drained and kept in plastic bucket at ambient temperature. Sampling was done at 0, 12 and 24 h storage and analysis was carried out organoleptically, chemically (TVB) and microbiologically (TPC, Enterobacter). Results showed that organoleptically, chemically and microbiologically, there were no significant different on spoilage inhibition of glyroxyl compared to control. Similar to control, glyroxyl was able to prevent spoilage of carp fish only up to 12 h. The fish was rejected by panelists after 12 h of storage. The TVB value and bacterial counts, including Enterobacter, also increased sharply after that time.
Isolasi, Purifikasi dan Immunogenitas Protein Outer Membran Vibrio Alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) Desrina Desrina; Arief Taslihan; Ambariyanto Ambariyanto; Ervia Yudiati; Yulius Docang Casessar; Raden Bagus Sugio Sumanta; Triyanto Triyanto; Hotnida Junita Situmeang; Langkah Sembiring
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.57

Abstract

The main objectives of this research were to isolate, purify and determine the immunogenicity of the outer membrane protein of V. alginolyticus. The outer membrane protein was isolated by sonication, sodium dodecyl sulphate-polyacrylamide agarose gel electrophoresis (SDS-PAGE) and purified by electroelusion method. Four outer membrane proteins were obtained: namely 32.0; 37.83; 64.13 and 73.43 kDa. Its immunogenicity in grouper Epinephelus fuscoguttatus was compared to that of bacterin of the same isolate. The immunogenicity test was conducted by intra peritoneal injection method. Each protein was dissolved in sterile Phospate Buffer Saline (PBS) and Freund’s Complete Adjuvant (FCA) (1:1) and injected 5 µg/ fish (fish weight 10-15 g). Bacterin (106, 107 and 108 cells/ml) was prepared in the same manner and injected at dosage of 0,2 ml/fish. Control fish were injected with 0.2 ml sterile PBS (pH 7.2). Booster was done a week later by injecting protein or bacterin with the Freund’s Incomplete Adjuvant (FIA). The agglutination test of antibody produced recognized cell surface protein of the whole cell of V. alginolyticus. Outer membrane protein 73.43 kDa was more immunogenic than the rest of proteins and bacterin, based on agglutinating antibodi titer.
Variasi Genetik Ikan Hias Clown, Amphiprion ocellaris Sari Budi Moria; Ida Komang Wardana; Gusti Ngurah Permana; Ahmad Muzaki; Ketut Maha Setiawati
Jurnal Perikanan Universitas Gadjah Mada Vol 9, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (419.522 KB) | DOI: 10.22146/jfs.62

Abstract

Evaluation of genetic variation of wild clown fish, Amphiprion ocellaris was conducted to collect basic knowledge in order to develop sustainable ornamental fish breeding under controled condition. Clown fish samples were collected from three locations, i.e. Bali, Madura, and South Sulawesi waters. PCR amplification of mt-DNA by using universal primer obtained single band with molecule weight of 461 bp. RFLP of mt-DNA with Hinf I, Mbo I and Nla III restriction enzymes showed that the highest genetic variation as 0.581 was found from South Sulawesi population and the lowest as 0.382 from Bali, while Madura population has genetic variation of 0.456. Genetic distance of clown fish from South Sulawesi and Madura was closer (0.107) comparing to clown fish from Bali population (0.270).

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