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INDONESIA
JOURNAL OF COASTAL DEVELOPMENT
Published by Universitas Diponegoro
ISSN : 14105217     EISSN : -     DOI : -
Core Subject : Education,
The Journal of Coastal Development (ISSN 1410-5217) is dedicated to all aspects of the increasingly important fields of coastal and marine development, including but not limited to biological, chemical, cultural, economic, social, medical, and physical development. The journal is jointly published by the Research Institute of Diponegoro University, Semarang, Indonesia and Indonesian Assosiation of Oceanologist (ISOI).
Arjuna Subject : -
Articles 328 Documents
STUDY ON THE SIZE STRUCTURE AND POPULATION PARAMETERS OF MUD CRAB SCYLLA SERRATA IN LAWELE BAY, SOUTHEAST SULAWESI, INDONESIA La Sara
JOURNAL OF COASTAL DEVELOPMENT Vol 13, No 2 (2010): Volume 13, number 2, Year 2010
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Abstract

Population dynamics of mud crab Scylla serrata were studied in Lawele Bay.  The objectives were to determine population size structure and population parameters. Samples were obtained monthly using gillnets and baited traps.  Less than 70% of gillnet catches during flood tide consisted of adults. Subadults and juveniles were 28.0% and 4.5%, respectively.  Similarly, during ebb tide consisted of 51.7%, 24.2% and 24.1%, respectively.  It suggests that gillnets are not size selective gear. In contrast, baited traps only caught adults and subadults with percentage of 90.5% and 9.5%, respectively.  The CW¥ and K of males were slightly higher than females namely 21.147 and 1.38 for males and 21.023 and 0.83 for females.  The mortality estimates are as follows: natural mortality (M) male = 2.48 and fishing mortality (F) male = 1.2, while M female = 1.78 and F female = 0.75.  The difference was due to CW¥, K and Z.  The results show that S. serrata population is still under exploited.    
A PILOT STUDY FOR THE ESTIMATION OF ABOVE GROUND BIOMASS AND LITTER PRODUCTION IN Rhizophora mucronata DOMINATED MANGROVE ECOSYSTEMS IN THE ISLAND Of MAURITIUS Shafiiq Abib; Chandani Appadoo
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 1 (2012): Volume 16, Number 1, Year 2012
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Abstract

Estimation of the above ground biomass of mangroves is an important issue because of its relevance to nutrient turnover and the potential to store carbon. Productivity, as indicated by litter fall rates is important to determine the rate at which mangroves produce new biomass. The aim of the current study was to estimate the above ground biomass and quantify litter fall in two Rhizophora mucronata dominated mangrove ecosystems of Mauritius located at Trou D’eau Douce (eastern side), and  at Petite Riviere Noire on the (western side) of the island. Field studies were conducted from September 2011 to January 2012. Quadrats of 5 × 5 metres were set up in each site and 200 mature trees were surveyed. Sixteen litter traps were constructed and installed at the two sites for litter collection. To estimate the above ground biomass, allometric equations were used. The total above ground biomass for Trou D’eau Douce and Petite Riviere Noire were 26.96 t ha-1 and 16.63 t ha-1, respectively. The mean rate of litter fall for Trou D’eau Douce and Petite Riviere Noire were 3.2 ± 0.44 g DW m-2 day-1 and 4.07± 0.95 g DW m-2 day-1, respectively. This study is among the first to provide information on the estimation of above ground biomass for mangroves of Mauritius. It is also the first to provide data on the litter production in mangroves at Petite Riviere Noire and Trou D’eau Douce.
CORALS AS SOURCE OF BACTERIA WITH ANTIMICROBIAL ACTIVITY ocky radjasa; Jutta Wiese; Agus Sabdono; Johannes F Imhoff
JOURNAL OF COASTAL DEVELOPMENT Vol 11, No 3 (2008): Volume 11, Number 1, Year 2008
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Abstract

In this study we examined marine bacteria associated with different corals (Porites lutea, Galaxea fascicularis, Acropora sp. and Pavona sp.) collected from vicinity of Panjang island, Jepara, North Java Sea, Indonesia for their antimicrobial activities against the bacteria Echerichia coli, Bacillus subtilis, Staphylococcus lentus and the yeast Candida glabrata. A total of 13 bacterial isolates belonged to the members of Bacillus, Vibrio, Micrococcus, Pseudoalteromonas, Arthrobacter and Pseudovibrio were found to inhibit the growth of at least one test strain. Further examinations among the biologically active strains by using PCR with specific primers of non-ribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) resulted in the presence of NRPS gene fragments in the 2 members of Bacillus and Micrococcus and PKS gene fragments in the 2 members of Bacillus and Vibrio. Following cloning and sequencing of the PCR products, the fragments from Bacillus BM1.5 and Micrococcus BJB showed sequence identity with peptide synthetase genes of Bacillus subtilis (61 %) and Actinoplanes teichomyceticus (62.4%). On the other hand, PKS-amplifying strains Bacillus BJ.7 and Vibrio MJ.5 showed closest sequence identity with polyketide synthase genes of Bacillus subtilis (73%) and Anabaena sp 90 (62%), respectively.
THE EFFECT OF CHITIN AND CHITOSAN OF CRAB SHELL ON WATER SORPTION OF ISOTHERM AND DENATURATION OF MYOFIBRILS DURING DEHYDRATION PROCESS Y. S. Darmanto
JOURNAL OF COASTAL DEVELOPMENT Vol 5, No 2 (2002): Volume 5, Number 2, Year 2002
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Abstract

Indonesian shrimp production is estimated at approximately  342,000 tons per year, followed by crab production at the level of more than 200,000 tons annually. Apparently, 50 – 60% out of the total production consists  of waste in the form of crab shell.  Crab shell is  rich in chitin, chitosan, and cellulose. The United States, Japan and other  developed industrial  countries have used chitin, chitosan, and cellulose as  raw material for various purposes, such as toxic waste processing, water purification, enzyme immobilization, skin and hair cosmetics, bone connecting, biomedicine, paper and textile industry, pharmacology, film, food industry, feed and others Chitin (C8H13NO5) is a Poly-b-N-Acetyl-D-GlucoSamine standing for a natural biopolymer, which constructs the shells of crab species. Chitin cannot be examined as a pure essence, since it is melt with rich texture of protein, CaCO3, fat pigmen, and small amount of metals. In order to fabricate Chitosan, one should demolish the Acetyl cluster of Chitin by employing strong alkalis. Chitin makes up the combination between Poly (N-acetyl-2-amino-2-deoxy-b-D-gluco-piranosa) and N-acetyl-2-amino-2-deoxy-D-glucopiranosa. To find out the effect of chitin and chitosan from crab shell on the water sorption isotherm of myofibrils protein during dehydration process, chitin and chitosan from crab shell were added to myofibrils protein at the ratio of ratio 2,5 – 7,5 g / 100 g,  homogenized, and afterwards dried in a dessicator. After some time, moisture content, water activity (Aw), Ca-ATPase activity, and proximate were analyzed. Mono layer water was analyzed according to Brunauer’s method (1968), multi-layer water was analyzed according to Bull’s method (1944), whereas Ca-ATPase activity was analyzed using the formula introduced by Katoh et. al. (1977). The result of the analyses shows that a higher concentration of chitin and chitosan on myofibrils resulted in higher amount of mono-layer and multi-layer water. The presence of different amount of mono-layer and multi-layer water indicates that the state of water changing occurs on myofibrils protein which in turn affects its quality. Likewise, the  increase of chitin and chitosan concentration  suppresses the  decrease of  acceleration of Ca-ATPase activity.
THE INITIAL ADSORPTION OF Pb2+ TO Dunaliella salina Moh. Muhaemin
JOURNAL OF COASTAL DEVELOPMENT Vol 9, No 2 (2006): Volume 9, Number 2, Year 2006
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Abstract

The adsorption process of metal ion to algal sudace groups and complexes to become organic material at all growth phases could be ajecting their behaviour in seawater. The dwerential pulse anodic stripping voltammetry (DPASIO method was used to determine the adsorption and interaction between the Dunaliella salina and Pbz`. The rate of adsorption was found in two steps; first a relative fast adsorption step (I0 min) and second slower or aw'usion—controlled uptake into the cells. The parabolic equation could be performed ([Pb2+]mr,a = L3 I x I0'7 + 0.062 x 107 V0 to describe the relationship between metal ion adsorption and uptake by the algae as a function of time. The high ajfinity constant is similar to the complexing capacity by the alga and no signylcant effect of cell density on binding capacity. This shows that ligands produced by the algae play an important part in buffering concentration of free metal concentration
MARINE BIODISCOVERY RESEARCH IN INDONESIA : CHALLENGES AND REWARDS Ekowati Chasanah
JOURNAL OF COASTAL DEVELOPMENT Vol 12, No 1 (2008): Volume 12, Number 1, Year 2008
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Abstract

Marine biodiscovery or bioprospecting activity is a search for marine products derived from marine biodiversity that can be developed for various industrial needs. Including in this activity is the process of identifying chemical compounds made by biological organisms which is often called natural product discovery. Indonesia, well known as a mega-diversity country, is one of the world hot sport of marine biodiversity. The richness of biodiversity is claimed as mirror of the richness of the chemical compounds, therefore, Indonesian waters might be rewarded with variety of chemical compounds thought to be an endless source of novel drugs and drug leads for pharmaceutical use. Up to 2007, at least 77 new compounds from 14 sponges and 19 new compounds from non-sponge organisms with pharmacological potential have been identified from Indonesian waters. To make this richness potentials becoming real in economic value, many factors should be considered. The bioactive is produced in small quantity, and the lengthy process from discovery step of a novel compound to the preclinical and clinical trials step is usually becoming a problem. Mari culture might be one among methods that can be developed in Indonesia to overcome the degradation hazard of marine resources. Conducive environment for investments, and improvement of technology on marine bioactive production through mariculture are factors to be improved to initiate and develop a sustainable biotechnology industries in Indonesia. 
NOVEL MOLECULAR METHODS FOR DISCOVERY AND ENGINEERING OF BIOCATALYSTS FROM UNCULTURED MARINE MICROORGANISMS Agustinus Robert Uria; Yusro Nuri Fawzya; Ekowati Chasanah
JOURNAL OF COASTAL DEVELOPMENT Vol 8, No 2 (2005): Volume 8, Number 2, Year 2005
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Abstract

Metagenomics is a powerful cultivation-independent approach, which can be applied to gain access to the biocatalysts from uncultured marine microorganisms. Discovery of marine biocatalysts by this approach, in general, involves four main steps. First, a metagenomic library containing a pool of biocatalyst-encoding genes is constructed from a marine environment, which can be done by various methods, including cloning of enzymatically-digested DNA, uncut DNA, and PCR-amplified products. Second, the metagenomic library is screened for the genes of interest by employing the activity assay of expression product, in situ  hybridization, or Polymerase Chain Reaction (PCR). Third, the obtained target genes, both functional and phylogenetic genes, are sequenced and analysed by using bioinformatic tools in order to gain information on the functional and structural properties as well as the microbial sources of the encoded biocatalysts. Finally, the target genes are expressed in suitable microbial hosts, thereby producing the corresponding recombinant biocatalysts. All existing methods in engineering of marine biocatalysts for the performance improvement can be classified into two main strategies: (i) rational design and (ii) directed evolution. Rational design, which may include the use of resctriction enzyme(s) and splicing by overlap extension (SOE), requires information on the biocatalyst`s structural and functional properties to alter specific amino acid(s). Whereas directed evolution, including error-prone PCR technique and gene shuffling, needs no such information.
SCREENING OF SECONDARY METABOLITE-PRODUCING BACTERIA ASSOCIATED WITH CORALS USING 16S rDNA-BASED APPROACH Ocky Karna Radjasa; Agus Sabdono
JOURNAL OF COASTAL DEVELOPMENT Vol 7, No 1 (2003): Volume 7, Number 1, Year 2003
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Abstract

A total of 42 coral-associated bacteria were screened for their ability to produce secondary metabolites by using 16S rDNA-based approach. A specific oligonucleotides primer was used to amplify DNA non-ribosomal peptide synthetase (NRPS). Five strains were found to carry the NRPS gene and successfully inhibit the growth of other coral-associated and pathogenic bacteria. The phylogenetic characterization revealed that four strains belonged to currently known species Pseudoalteromonas luteoviolacea, Vibrio tubiashi, Vibrio carchiariae and Bacillus iodinum. On the other hand, one strain was apparently closely related with an unknown group of the gamma Proteobacteria, which was only recognized as uncultured clone 141H2.
PROSPECT OF SEA CUCUMBER CULTURE IN INDONESIA AS POTENTIAL FOOD SOURCES Henneke Pangkey; Sartje Lantu; Lusia Manuand; JeffrieFredrik Mokolensang
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 2 (2012): Volume 15, Number 2, Year 2012
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Abstract

Sea cucumber is one of the sea treasures which has been used not only as luxury food for certain countries but also as medicines. Sea cucumber has become one of the most important products and it has high price in international market.For this reason, the exploitation of sea cucumber turns out to be excessive and disturbs its sustainability. Aquaculture is the best way to prevent this problem. The success of sea cucumber culture is very depend on seed availability and suitable food for larvae as well as juvenile along with growing out stadia for market requirement.
THE GROWTH OF TWO MARINE-MICROALGAE (Chaetoceros gracilis and Tetraselmis chuii ) IN SEMI-CON TINUOUS CULTURE METHOD Subandiyono .
JOURNAL OF COASTAL DEVELOPMENT Vol 1, No 2 (1998): Volume 1, Number 2, Year 1998
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Abstract

The growth performance of two marine-microalgal species, i.e. a centric diatom- Chaetoceros gracilis and a green algae- Tetraelmis chui, were investigated. Semi continuous- 200 litre plastic bag facilitated with a 0.2 mm hydrophobic filter membrane for each were required to culture each species of the microalgae. Aseptical method was employed during algal culture, harvesting, and refilling. The algal density in the bag culture was monitored every 2 days. Results indicated that the average value of mean generation time for C. gracilis and T. chui were 18.83 and 25.14 hours, respectively. The average value of bacterial counts taken from inoculated C. gracilis and T. chui culture samples were 9.9 x 10E6 and          7.8 x 10E4 bacteria ml media. The importance of bacterial monitoring in relation to recommended culture method is discusses briefly.

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