Biosaintifika: Journal of Biology & Biology Education
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
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<![CDATA[IDENTIFYING THE POTENTIAL YIELD OF SOYBEAN GENOTYPE IN TIDAL LAND TYPE C ]]>
<![CDATA[Susanto, Gatut Wahyu Anggoro]]>;
<![CDATA[Putri, Pratanti Haksiwi]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.21395
<![CDATA[Identification of soybean genotypes including potential yield character is a base to understand soybean growth, development, and productivity on potential lands. The purpose of this research is to determine the potential yield of soybean genotype in tidal land type C and their supporting components. Four soybean genotypes and two comparative varieties Anjasmoro and Lawit (adaptive tidal land) were tested on tidal land type C at four locations (three locations in Jambi Province and one location in South Kalimantan Province), July 2017. The research was conducted using a randomized design for each location and repeated four times. The plot size was 12 m2, spacing row 40 cm x 15 cm, two plants for each hole. GH_KR_13, GH_GR_1 and GH_GR_5 productivity equal with Lawit, the adaptive variety for tidal land type C. While, Anjasmoro showed more than 2.20 t/ha in seeds productivity, 54 filled pod for each plant, with large size of seeds (15.84 gram/100 seeds). Anjasmoro had higher seed productivity than all of genotypes including Lawit. Anjasmoro with it?s potential yield and productivity, can become farmer preferences and deserve to be developed in tidal land type C. ]]>
<![CDATA[GENETIC DIFFERENCE BETWEEN TWO PHENOTYPICALLY SIMILAR MEMBERS OF ASTERACEAE BY THE USE OF INTERGENIC SPACER ATPB – RBCL ]]>
<![CDATA[Susanto, Agus Hery]]>;
<![CDATA[Dwiati, Murni]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.22137
<![CDATA[Two Asteraceae species, i.e. Synedrella nodiflora (L.) Gaertn and Eleutheranthea ruderalis (Swartz) Sch.-Bpi. are phenotypically similar with each other, although some differences in morphological and anatomical traits are apparently observable. Molecular comparison using particular marker is required to support a phenotype-based study that previously reported. Chloroplast DNA marker, . atpB ? rbcL IGS, was used to identify genetic difference between both species. Six samples of the respective species were collected randomly from some places in Banyumas Regency, Central Java, Indonesia. Amplification of the marker was performed employing a pair of universal primers. Sequence alignment on the PCR products showed that no difference in atpB ? rbcL IGS sequences, either within S. nodiflora or E. ruderalis samples was observed. On the other hands, several deletions and base substitution in both S. nodiflora and E. ruderalis were detected when alignment was made between both species. This result suggests that they reveal a convincing genetic difference. Inspite of no direct correlation between this genetic and some visible phenotypic differences, this finding provides preliminary scientific background on the phenotypic traits of both species, which are often difficult to find at a rapid observation.]]>
<![CDATA[Digestive Enzyme Activities of Osteochilus vittatus with Spirulina platensis Feed Supplementation in Biofloc System ]]>
<![CDATA[Rizka Yunida]]>;
<![CDATA[Purnama Sukardi]]>;
<![CDATA[Sorta Basar Ida Simanjuntak]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.19108
<![CDATA[Osteochilus vittatus is a freshwater fish that needs to be developed because it tastes savory and can be used as a biocleaning agent. Research on the effect of supplementation of Spirulina platensis in nilem fish that is maintained in a biofloc system on digestive enzyme activity has never been done before. The objectives of this study were to determine the effect of S. platensis supplementation on Osteochilus vittatus digestive enzyme activity maintained in the biofloc system and to obtain the most optimum level of S. platensis supplementation in improving the  digestive enzymes activity. The study was conducted experimentally with Completely Randomized Design with four treatments and five replications. Osteochilus vittatus were fed with Spirulina platensis level of 0, 2, 4, and 6 g kg-1 for 56 days. Enzyme activity was measured on days 0 and 56 with three kinds of buffer solutions. Amylase and protease activity was analyzed using ANOVA with a confidence level of 95%. The results showed that the highest amylase activity in the liver was 4.764 ± 1.705 U mg-1 protein and proximal intestine was 2.328 ± 0.838 U mg-1 protein. The highest protease activity was found in the liver and proximal intestine with activity of 6.536 ± 0.911 U mg-1 protein and 6.207 ± 2.195 U mg-1protein. Spirulina platensis supplementation level of 6 g kg-1 feed is the most optimum level (p0.05). Cultivation of fish with S. platensis supplementation in feed can increase enzyme activity and the fish maintenance in biofloc systems can improve water quality.]]>
<![CDATA[Phytochemical and Cytotoxic Evaluation of Krangean Fruits Extracts Against HeLa, MCF-7, and HepG2 Cancer Cell Line ]]>
<![CDATA[Yuli Widiyastuti]]>;
<![CDATA[Ika Yanti Marfuatush Sholikhah]]>;
<![CDATA[Sari Haryanti]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.18073
<![CDATA[Krangean [Litsea cubeba (Lour.) Pers.] is one of ancient aromatic plants in Indonesia which is used as traditional medicines such as for carminative, stimulant, stomach ache and expectorant. Otherwise, the anticancer activity of this plant has not been explored extensively. This research aimed to investigate phytochemical content and cytotoxic activity of krangean fruits extract on human cancer cell line in vitro. The research was an in vitro experimental design and the cytotoxic activity was carried out with MMT assay. The phytochemical compounds were characterized by TLC (Thin Layer Chromatography). MTT assay was done to observe morphology and viability of HeLa cervical cancer, MCF-7 breast cancer, and HepG2 liver cancer cell line. The results showed that TLC characterization of chloroform and methanolic extracts of Litsea cubeba revealed similar profile, with the major compound found were terpenoid and alkaloid. The MTT assay found that both extracts had strong inhibition on HeLa cell line. Chloroform extract exhibited stronger cytotoxic activities compared to methanol, with the IC50 values of 37.3 and 64.7 μg/mL respectively. While, the both extract have moderate cytotoxic activities to HepG2 and MCF-7 cancer cell line indicated by IC50value more than 100 mg/mL. The benefit of this study is to provide the scientific information regarding the potency of krangean fruit as herbal natural medicine for cervical cancer therapy.]]>
<![CDATA[Application of Bio P60 and Bio T10 in Combination Against Phytophthora Wilt of Papaya ]]>
<![CDATA[Loekas Soesanto]]>;
<![CDATA[Kustam Kustam]]>;
<![CDATA[Endang Mugiastuti]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.20389
<![CDATA[Papaya is one of the most widely cultivated horticultural plants. Phytophthora wilt is an important papaya disease which results in production losses. This research aimed to determine the effect of Bio P60 (raw secondary metabolites of Pseudomonas fluorescens P60) and Bio T10 (raw secondary metabolites of Trichoderma harzianum T10) application in combination on Phytophthora wilt and on growth of pepaya. The research was conducted on Californian pepaya farm, Linggasari Village, Kembaran District, Banyumas Regency. Randomized block design was used with six replicates and five treatments consisted of control (Mancozeb 80%), Bio T10 flush + Bio T10 spray, Bio T10 flush + Bio P60 spray, Bio P60 flush + Bio T10 spray, and Bio P60 flush + Bio P60 spray. Variables observed were percentage of healthy leaves, infection rate, number of healthy leaves, and number of healthy pepaya fruit. Result of the research showed that application of Bio P60 and Bio T10 effectively cured Phytophthora wilt with the percentage of healthy leaves as 69.19% compared to control. The combination was able to increase the number of healthy leaves the number of healthy papaya fruit. The novelty of this research is that raw secondary metabolites of biological agents proven to be able to overcome the papaya diseases, which so far cannot be solved. The implication for the development of science is one step ahead in overcoming plant diseases biologically by utilizing raw secondary metabolites. The benefits for the community can overcome papaya plant diseases organically, safely, and environmentally friendly, and inexpensive.]]>
<![CDATA[The Dominance of Tramps Ants in The Settlement Area of Semarang, Central Java ]]>
<![CDATA[Ivan Mahadika Putra]]>;
<![CDATA[Mochammad Hadi]]>;
<![CDATA[Rully Rahadian]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.21026
<![CDATA[The high rate of urbanization in Semarang have caused the land conversion from forest into various human need spaces. The land conversion is not only affect the life of humans but also the animals, including ants. The objectives of this study were to examine the dominance of tramp ants in the settlement area of Semarang City. The method used was the bait trap method using chicken intestine as a bait. Samples of ants were collected from four habitats, i.e., waste disposal area, traditional markets, house area, and urban parks. This study found four dominant ants, i.e., Anoplolepis gracilipes, Paratrechina longicornis, Solenopsis geminata and Monomorium pharaonis. Two of them were categorized as tramp ants (Anoplolepis gracillipes and Paratrechina longicornis). The highest diversity of ants was found in house (H'= 2.72). The waste area tends to be an ideal habitat for particular species, which was proved by the low value of Simpson's dominance index (iD = 0.06) and a high value of evenness index (E=0 0.89). This study provides new information about tramp ants population as pest in urban area. The findings would be beneficial for controlling the population of tramp ants, which are disturbing humans in the settlement area.]]>
<![CDATA[The Effect of Aloe Vera Peel Extract on Histopathology of Rat Pancreas Induced by Alloxan ]]>
<![CDATA[R Susanti]]>;
<![CDATA[Eka Setiadi]]>;
<![CDATA[Endah Peniati]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.20896
<![CDATA[The Extract of Aloe Vera Peel (EAVP) contains bioactive compounds (flavonoids, alkaloids, tannins, saponins, and phenolics) that it thought to improve pancreas histopatology on rat with diabetes mellitus, therefore it has potential for diabetes mellitus treatment. This research aimed to figure out the effect of EAVP on improving the histopathology of rat pancreas induced by alloxan. This research was an experimental study using a completely randomized design with a randomized post-test design. A total of 25 rats were divided into 5 groups: C(-) was a normal group, fed and drinking standard; C(+) was positive control group, induced by alloxan 120 mg/kgBW; PI, PII and PIII were groups that were induced by alloxan 120 mg/kgBW and were given a full-dose of EAVP of 87.5, 175 and 350 mg/kgBW respectively. The data was assessed using the Kruskal-Wallis and Mann-Whitney statistical analysis. The results of the statistical test showed that the histopathology of rat pancreas of the C(+) group were significantly different compared with the treatment group. Meanwhile, the representation of histopathology of pancreas between PIII and C(-) were not significantly different. It can be concluded that treating hyperglycemia rats with the EAVP for 28 days can improve the representation of histopathology of rat pancreas. At the laboratory level, EAVP has been shown to repair rat pancreatic damage. With this result, Aloe vera has the potential to be developed as a phytopharmaca for the prevention or treatment of diabetes mellitus.]]>
<![CDATA[Effects of Kersen Juice and Lakum Leaf Extract on Lipid Profile of White Rats With Hyperlipidemia ]]>
<![CDATA[Didin Sopandi]]>;
<![CDATA[Tyas Rini Saraswati]]>;
<![CDATA[Enny Yusuf Wachidah Yuniwarti]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.20813
<![CDATA[Kersen fruit and Lakum leaf contain substances that are potential as an antihyperlipidemic. The aim of this research was to analyze the effects of Kersen juice (Mutingia calabura L.) with dosage 0.2 ml/200 g BW and lakum leaf extract (Cayratia trifolia L.) with dosage of 40 mg/200 g BW on lipid profile of white rats (Rattus norvegicus L.) with hyperlipidemia. This study used a Complete Randomized Design (CRD). This study used twenty adult male Wistar rats which were divided into five groups : P0 as a control group, P1 as a High Feed Diet (HFD) control group, P2 as a HFD group treated with Kersen juice with dosage of 0.2 ml/200 g BW, P3 as a HFD group treated with Lakum leaf extract with dosage of 40 mg/200 g BW and P4 as a HFD group treated with simvastatin. The treatments were given orally for 28 days. The parameters of this study were the level of cholesterol, HDL, LDL and triglyceride, as well as body weight, and food consumption. One-way ANOVA test was performed to determine the significant differences p0.05 between groups followed by Duncan test with a 95% significant level. The results showed that compared to Kersen juice with dosage of 0.2 ml/200 g BW, administration of Lakum leaf extract with dosage of 40 mg/200 g BW was more effective in lowering cholesterol level, triglyceride and increasing HDL level, however, they were not effective in lowering LDL in rats with hyperlipidemia. This result shows that kersen and lakum leaf could be an alternative to prevent and avoid further effects of high cholesterol in people with hyperlipidemia.]]>
<![CDATA[Identifying the Potential Yield of Soybean Genotype in Tidal Land Type C ]]>
<![CDATA[Gatut Wahyu Anggoro Susanto]]>;
<![CDATA[Pratanti Haksiwi Putri]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.21395
<![CDATA[Identification of soybean genotypes including potential yield character is a base to understand soybean growth, development, and productivity on potential lands. The purpose of this research is to determine the potential yield of soybean genotype in tidal land type C and their supporting components. Four soybean genotypes and two comparative varieties Anjasmoro and Lawit (adaptive tidal land) were tested on tidal land type C at four locations (three locations in Jambi Province and one location in South Kalimantan Province), July 2017. The research was conducted using a randomized design for each location and repeated four times. The plot size was 12 m2, spacing row 40 cm x 15 cm, two plants for each hole. GH_KR_13, GH_GR_1 and GH_GR_5 productivity equal with Lawit, the adaptive variety for tidal land type C. While, Anjasmoro showed more than 2.20 t/ha in seeds productivity, 54 filled pod for each plant, with large size of seeds (15.84 gram/100 seeds). Anjasmoro had higher seed productivity than all of genotypes including Lawit. Anjasmoro with it’s potential yield and productivity, can become farmer preferences and deserve to be developed in tidal land type C. ]]>
<![CDATA[Ethnobotany of Balimo (Zanthoxylum nitidum) in the Kanayatn Dayak Community in Tapakng, West Kalimantan ]]>
<![CDATA[Letus Sepsamli]]>;
<![CDATA[Jumari Jumari]]>;
<![CDATA[Erma Prihastanti]]>
<![CDATA[ Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019 ]]>
Publisher : <![CDATA[Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro ]]>
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DOI: 10.15294/biosaintifika.v11i3.20688
<![CDATA[The Kanayatn Dayak in Tapakng Village has a local culture of using balimo plants for traditional medicine. Balimo is a plant, it has an important meaning in traditional medicine by the Kanayatn Dayak community in Tapakng Village. This study aimed to examine the ethnobotanical knowledge of balimo plants and their advantage among the Kanayatn Dayak  community in Tapakng Village. The research method with observation and deep interview and also semi-structured interview. Deep interviews was conducted with four key informants and the semi-structured interview was conducted with 60 respondents. The results showed that, the local people had a traditional treatment system to solve the disease problems. Balimo plants are used as a prevention of alcohol intoxication due to drinking arak and treatment of coughs (dry cough and bloody cough or (hemoptysis). The use of balimo as a prevention of intoxication has an important role because it is related to the Dayak Indigenous rituals. The level of ethnobotanical knowledge of local people showed that, 100% have heard about the balimo, 92% have seen balimo, and 55% have used it. This research provided information to the public about balimo that can be used in medication. Information on the utilization of balimo gives awareness to the people, the population of balimo really needs to be maintained in nature from extinction.]]>
