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All Journal Pembangunan Pedesaan Biosaintifika: Journal of Biology & Biology Education Acta Biochimica Indonesiana BioEksakta : Jurnal Ilmiah Biologi Unsoed
Murni Dwiati
Fakultas Ekonomi Universitas Jenderal Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Immunology & microbiology Veterinary Other

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PERTUMBUHAN DAN HASIL TANAMAN CAISIN (Brassica chinenchis L.) PADA BERBAGAI JENIS PUPUK ORGANIK DAN PUPUK FOSFAT ALAM THE GROWTH AND PRODUCTION OF CAISIN (Brassica chinenchis L.) ON VARIETIES OF ORGANIC FERTILIZERS AND ROCK PHOSPHATE Kusumasari, Aryana Citra; Budisantoso, Iman; Dwiati, Murni
Pembangunan Pedesaan Vol 4, No 2 (2004)
Publisher : Pembangunan Pedesaan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Tujuan penelitian adalah untuk mengetahui pengaruh pemberian pupuk organik dan fosfat alam terhadap pertumbuhan dan hasil tanaman caisin, serta menentukan jenis pupuk organik dan dosis pupuk fosfat alam terbaik dalam memacu pertumbuhan dan hasil tanaman caisin. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok dengan pola perlakuan Petak Terpisah (Split Splot Design). Petak utama berupa jenis pupuk organic yaitu pupuk kandang kambing (A ), pupuk kandang sapi (A ), dan pupuk 1 2 limbah media jamur merang (A ) dengan dosis masing-masing 10 ton/ha. 3 Anak petak berupa dosis fosfat alam 0 (B ), 250 (B ), 350 (B ), dan 450 (B ) 0 1 2 3 kg/ha, sehingga terdapat 12 kombinasi perlakuan dan setiap perlakuan diulang 3 kali. Parameter yang diamati meliputi parameter pertumbuhan yaitu jumlah daun, luas daun, bobot kering, nisbah pupus akar dan parameter hasil yaitu bobot basah daun. Data yang diperoleh dianalisis dengan uji F, apabila berbeda nyata dilanjutkan dengan uji DMRT. Hasil penelitian menunjukkan bahwa pemberian pupuk organik dan fosfat alam berpengaruh terhadap pertumbuhan dan hasil tanaman caisin. Pupuk limbah media jamur merang dan fosfat alam dosis 450 kg/ha (A B ) merupakan kombinasi terbaik dalam 3 3 memacu pertumbuhan dan hasil tanaman caisin.
GENETIC DIFFERENCE BETWEEN TWO PHENOTYPICALLY SIMILAR MEMBERS OF ASTERACEAE BY THE USE OF INTERGENIC SPACER ATPB – RBCL Susanto, Agus Hery; Dwiati, Murni
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): December 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.637 KB) | DOI: 10.15294/biosaintifika.v11i3.22137

Abstract

Two Asteraceae species, i.e. Synedrella nodiflora (L.) Gaertn and Eleutheranthea ruderalis (Swartz) Sch.-Bpi. are phenotypically similar with each other, although some differences in morphological and anatomical traits are apparently observable. Molecular comparison using particular marker is required to support a phenotype-based study that previously reported. Chloroplast DNA marker, . atpB ? rbcL IGS, was used to identify genetic difference between both species. Six samples of the respective species were collected randomly from some places in Banyumas Regency, Central Java, Indonesia. Amplification of the marker was performed employing a pair of universal primers. Sequence alignment on the PCR products showed that no difference in atpB ? rbcL IGS sequences, either within S. nodiflora or E. ruderalis samples was observed. On the other hands, several deletions and base substitution in both S. nodiflora and E. ruderalis were detected when alignment was made between both species. This result suggests that they reveal a convincing genetic difference. Inspite of no direct correlation between this genetic and some visible phenotypic differences, this finding provides preliminary scientific background on the phenotypic traits of both species, which are often difficult to find at a rapid observation.
Induksi Kalus Nepenthes mirabilis(Lour.) Druce Menggunakan NAA danTDZ Nurussakinah, Nina; Dwiati, Murni; Budisantoso, Iman
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4242

Abstract

Nepenthes sp. has a characteristic in the form of a modified leaf tip into a pitcher. Nepenthes mirabilis (Lour.) Druce in its original habitat is widely hunted for sale. Propagation of N. mirabilis in nature has very little success rate, to overcome this it is necessary to propagate it by means of in vitro culture and use young leaf explants to form callus. Growth regulators such as Auxins (NAA) and Cytokinins (TDZ) can stimulate callus growth. Auxins can stimulate callus formation by stimulating cell expansion and cytokinins can stimulate the cell division process. The purpose of this study was to determine the effect of NAA and TDZ in inducing N. mirabilis callus and to determine the interaction between NAA and TDZ in inducing N. mirabilis callus. The experimental design used was a factorial CRD pattern, with two factors, namely NAA and TDZ. NAA consists of four levels, namely, 0 mg / L (N0); 0.5 mg / L (N1), 1 mg / L (N2) and 1.5 mg / L (N3). TDZ also consists of four levels, namely 0 mg / L (T0); 1 mg / L (T1); 2 mg / L (T2) and 3 mg / L (T3). Each treatment was repeated three times. The results showed that the addition of NAA and TDZ had an effect on callus induction of N. mirabilis. The TDZ concentration of 1 mg / L (T1) stimulated callus induction, especially the percentage of callus formation and callus diameter. Meanwhile, NAA 1 mg / L (N2) boosted the percentage of N. mirabilis callus formation.
APLIKASI NAA PADA PROPAGASI KANTONG SEMAR Nepenthes adrianii Batoro, Wartono & Jebb Wahdani, Rosa Amalia; Dwiati, Murni; Kamsinah, Kamsinah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.5481

Abstract

Nepenthes adrianii is an endemic species of Mount Slamet. The existence of this species has begun to be threatened. This situation encourages efforts to conserve N. adrianii, which is through propagation using stem cuttings of N. adrianii. In order for the propagation of stem cuttings to grow properly, it is necessary to add growth hormone (ZPT) from the auxin group such as NAA (Napthalene Acetic Acid). This research has been conducted to study the effect of NAA and its effective consentration for the growth of N. adrianiistem cuttings. This research was conducted using experimental methods. The experimental design used was a Randomized Completely Block Design (RCBD). The block in this study is total leaves per cuttings. Block 1 is the leafless cuttings, block 2 is cuttings with one leaf, block 3 is cuttings with two leaves, and block 4 is cuttings with three leaves. The NAA concentrations in this study are 0, 5, 10, 15, 20, and 25 ppm. There are a total of 24 experimental units. The variable observed was the growth of N. adrianii stem cuttings. Parameters observed in this study are days taken for sprouting, number of shoots, number of leaves, leaf length, and leaf width. The results were analyzed using Variety Analysis and then analyzed with Tukey's test at the test level of 0.05 at the time of significant treatment. The results showed that the application of NAA did not affect the growth of the stem cuttings of N. adrianii. The best concentration of NAA could not be determined because there was no effect of NAA on the growth of N. adrianii stem cuttings.
Pertumbuhan Anggrek Vanda limbata Blume ‘Jawa’ pada Aplikasi Flurprimidol dan GA3 Rahmawati, Aprilia; Budisantoso, Iman; Dwiati, Murni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 2 (2024): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.2.11978

Abstract

Anggrek merupakan anggota keluarga Orchidaceae dengan bunga yang indah. Salah satu jenis anggrek yang menarik adalah anggrek Vanda limbata 'Jawa'. Anggrek asli Indonesia ini banyak ditemukan di Pulau Jawa. Anggrek ini terdaftar di CITES dengan status appendix II. Pengawetan dapat dilakukan dengan metode kultur in vitro , yaitu teknik perbanyakan tanaman dalam lingkungan terkendali dan steril. Beberapa senyawa dapat ditambahkan ke dalam media kultur untuk menjamin pertumbuhan yang baik, salah satunya adalah flurprimidiol. Namun flurprimidol dapat menghambat biosintesis asam giberelat sehingga menurunkan tinggi tanaman, oleh karena itu pada penelitian ini juga ditambahkan GA 3 yang berperan dalam proses pemanjangan sel pada daerah sub apikal. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian flurprimidol dan GA 3 pada konsentrasi berbeda terhadap pertumbuhan planlet Vanda limbata 'Jawa' secara in vitro. Penentuan konsentrasi flurprimidol dan GA 3 yang tepat untuk mempengaruhi pertumbuhan planlet anggrek Vanda limbata 'Jawa' secara in vitro. Penelitian ini menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL) dengan pola faktorial dua faktor. Faktor pertama adalah flurprimidol (0; 1; 2 mgL-1), dan faktor kedua adalah GA 3 (0; 1; 2; 3 mgL-1). Parameter yang diamati adalah klorofil a, klorofil b, kandungan klorofil total, tinggi tanaman, jumlah daun, panjang dan lebar daun, jumlah akar dan panjang akar, serta kondisi lingkungan. Analisis data menggunakan analisis varian (uji F pada taraf uji 0,05 dan 0,01, dilanjutkan dengan uji DMRT (Duncan Multiple Range Test) menggunakan software SPSS pada taraf uji 0,05. Pemberian flurprimidol dan GA 3 dapat mempengaruhi pertumbuhan pantlet anggrek V .limbata secara in vitro.Penggunaan flurprimidol dan GA 3 meningkatkan kandungan klorofil a, klorofil b, dan klorofil total, serta panjang akar Perlakuan Flurprimidol 2 mg/L dan GA 3 2 mg/L merupakan perlakuan terbaik dalam mempengaruhi pertumbuhan V. limbata 'Java '.
Oryzalin-induced polyploidy in Vanda limbata (Blume): Phenotypic assessment Dwiati, Murni; Hasam, Wahyu Nur; Susanto, Agus Hery
Acta Biochimica Indonesiana Vol. 8 No. 1 (2025): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.197

Abstract

Background: Vanda limbata is a natural orchid species found on Java Island, commonly known as V. limbata 'Jawa.' Enhancing plant vigor is essential to increase its potential as an ornamental plant, with one promising approach being induced polyploidy using chemical mutagens such as oryzalin. Objective: This study aimed to evaluate the effect of oryzalin on inducing polyploidy in V. limbata cultured in vitro. Method: Oryzalin was applied at concentrations of 0, 25, 50, 75, and 100 µM, with five replications for each treatment, resulting in 25 experimental units. Several morpho-physiological and anatomical traits were measured as indicators of polyploidy. Results: The results demonstrated that oryzalin at 100 µM was the most effective concentration for inducing polyploidy in V. limbata cultured in vitro. This was particularly evident in traits such as reduced leaf length, increased leaf width, enhanced adventitious shoot formation, and enlarged stomatal width. Conclusion: Oryzalin, when applied at appropriate concentrations, can be effectively used to induce polyploidy in V. limbate.
Diversitas Morfologi Nepenthes spp. Dataran Rendah Hasil Budidaya Maryanti Sitio, Risma Leonny; Dwiati, Murni; Yuniaty, Alice; Ramadani, Didi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 2 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.2.15870

Abstract

Nepenthes merupakan tanaman karnivora khas dengan diversitas morfologi tinggi yang menarik untuk diteliti, khususnya dalam mendukung upaya konservasi ex situ. Studi ini bertujuan untuk mengkarakterisasi sifat morfologi kualitatif beberapa spesies Nepenthes dataran rendah yang telah dibudidayakan selama 3-4 tahun di Purwokerto, Banyumas, Jawa Tengah. Karakterisasi dilakukan secara deskriptif terhadap karakter visual utama. Hasil studi menunjukkan diversitas morfologi yang khas, khususnya dalam hal struktur dan morfologi kantong dengan setiap spesies menampilkan bentuk dan karakteristik kantong yang berbeda. Kata kunci: Budidaya, Dataran Rendah, Diversitas, Morfologi, Nepenthes
Co-Authors AGUS HERY SUSANTO Alice Yuniaty Aryana Citra Kusumasari Hasam, Wahyu Nur Iman Budisantoso Iman Budisantoso Kamsinah Kamsinah Maryanti Sitio, Risma Leonny Nurussakinah, Nina Rahmawati, Aprilia Ramadani, Didi Wahdani, Rosa Amalia