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Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi
Published by Universitas Islam Negeri Sumatera Utara
ISSN : 26213702     EISSN : 26217538     DOI : 10.30821
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 152 Documents
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Technology readiness predictors of AI integration: SEM-PLS evidence from pre-service biology teachers in Indonesia Triandro, Agil Zuhal; Raharjo, Raharjo; Asri, Mahanani Tri
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4992

Abstract

The rapid advancement of Artificial Intelligence (AI) requires educators to possess adequate technological readiness. However, current literature predominantly focuses on in-service teachers using general acceptance models, often overlooking whether traditional psychological inhibitors remain relevant for 'digital native' students in developing contexts. This study aims to analyze the technology readiness profile of pre-service biology teachers and investigate the predictive effect of Technology Readiness Index (TRI) dimensions Optimism, Innovativeness, Discomfort, and Insecurity on their readiness to integrate AI in biology teaching. Employing a cross-sectional survey design, data were collected from 200 active undergraduate students at Universitas Negeri Surabaya and analyzed using Structural Equation Modeling-Partial Least Squares (SEM-PLS). The model explains a substantial variance in AI readiness (R2 = 0.538). The results revealed that Optimism acts as the most dominant significant driver, followed by Innovativeness. Crucially, this study offers new evidence that psychological inhibitors (Discomfort and Insecurity) no longer significantly affect AI integration readiness among mature users, challenging common assumptions in early adoption literature. The findings suggest a paradigm shift where pre-service teachers are pragmatic users driven by perceived utility rather than fear. Therefore, curriculum developers and policymakers must shift strategies from anxiety mitigation to the creation of biology-specific "embedded AI" tools that demonstrate tangible pedagogical benefits.
In silico and in vitro analysis primers for antibiotic resistance gene of bacteria Ayuntya, Eva; Ariesaka, Kiky Martha; Prihanta, Wahyu; Husamah, Husamah; Permana, Fendy Hardian; Nuryady, Moh Mirza
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4313

Abstract

The prevalence of Extended-Spectrum Beta-Lactamase (ESBL) occurs due to excessive use of antibiotics, resulting in antibiotic resistance. Research on ESBL has been conducted in several Indonesian cities, such as Surabaya, Malang, and Jakarta which identified the presence of ESBL bacterial isolates. Limited information about blaCTX-M and blaVEB primer data in in silico analysis such as primer length, GC base content, melting temperature, and organisms attached to the gene, is an important thing that needs further research, because in silico data will affect the annealing temperature in the in vitro process using Polymerase Chain Reaction (PCR). The purpose of this study was to determine that blaCTX-M and blaVEB primers can be used to detect the presence of ESBL genes from amplification results in PCR. In silico primer analysis was carried out using BLAST primers from the NCBI website which would then be continued through in vitro primer analysis using PCR to see the presence of DNA bands. The results of in silico research showed that there were 27 organisms detected in the blaCTX-M gene and 37 organisms in the blaVEB gene. The results of in vitro analysis on blaCTX-M primers can be amplified at ± 400 bp, while blaVEB cannot be amplified. 
Response of Dayak onion (Eleutherine bulbosa Merr.) plant growth after application of biosaka from the Asteraceae family Asriati, Siti; Elimasni, Elimasni; Nurwahyuni, Isnaini
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4597

Abstract

Public interest in the use of medicinal plants continues to grow. One of the medicinal plants that has been developed is dayak union (Eleutherine bulboba Merr.), which is believed to be able to cure various diseases. This research aims to determine the best growth response of Dayak onion after the application of biosaka from the Asteraceae family. The main implications of biosaka for agriculture are significant cost efficiency in production, improved plant and soil health, and the promotion of sustainable and environmentally friendly farming by improving land ecosystems. Biosaka is made by taking 60 g of each plant and then grinding it until it is dark brown and slightly foamy. Biosaka extraction produces approximately 20 ml. The experiment was conducted at the Faculty of Mathematics and Natural Sciences, University of North Sumatra from December to March 2025. The design used in this experiment is a Completely Randomized Design (CRD) consisting of 5 treatment levels of biosaka concentration, namely 0 ml/L, 3.5 ml/L, 4.5 ml/L, 5.5 ml/L, and 6.5 ml/L with eight replications. The data obtained were analyzed using SPSS version 22 software with ANOVA (Analysis of Variance) test. If the treatment has a significant effect, it is followed by DMRT (Duncan Multiple Range Test). The research results show that the application of a biosaka concentration of 6.5 ml/L yielded the highest results and had a significant effect on plant height, number of leaves, tuber diameter and number of shoots and tubers per clump.
Solo Black Garlic fermented whey kefir improves sperm quality of male mice (Mus musculus) induced by lead acetate Ulfa, Risda Arba; Nursaidah, Dina; Umami, Muhimatul
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4708

Abstract

Globally, men experience a decline in total fertility, characterized by a significant decline in sperm quality in terms of concentration, motility, and morphology. Environmental factors can modulate this through exposure to toxic elements, such as the heavy metal lead, in the workplace. Lead can accumulate in nature and contaminate drinking water supply systems. A series of negative responses can occur and disrupt spermatozoa production and quality. Natural ingredients, such as solo black garlic (SBG), have the potential to maintain conditions in reproductive function against the adverse effects of lead. This study utilized SBG fermented with whey kefir to investigate its effect and optimal dosage on improving spermatozoa quality in male mice induced by lead acetate. The test animals were 25 Swiss Webster male mice aged 12-14 weeks, divided into five treatment groups (n = 5 per group), namely negative control (KN), distilled water; positive control (KP), lead acetate 75 mg/kg BW; and three SBG treatment groups given lead acetate (SBG-60, SBG-120, and SBG-180). SBG was given together with lead acetate at doses of 60, 120, and 180 mg/kg BW for 30 days. The results showed SBG-120 restored concentration (31.00 × 106/ml) and motility (80.83%) toward negative-control levels; morphology abnormalities decreased by 30.50%, which were not significantly different (p > 0.05) from the negative control. Therefore, SBG with a dose of 120 mg/kg BW is optimum for maintaining spermatozoa quality in male mice exposed to lead acetate.
Secondary metabolites from Lansium domesticum as potential anti-inflammatory iNOS inhibitors: An in silico study Oktariansyah, Yadi; Hanum, Laila; Habiburrahman, Muhammad
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4731

Abstract

Inflammation is a crucial mechanism by which the immune system combats disease. However, when it becomes uncontrolled, it can result in acute inflammatory disorders. Although non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for treatment, their associated side effects have prompted the search for safer, natural alternatives. One of the enzymes central to the progression of acute inflammation is inducible nitric oxide synthase (iNOS), particularly its active site, plays a key role in the progression of acute inflammation. Lansium domesticum Corrêa, 1807 have been reported to contain secondary metabolites with anti-inflammatory potential, but their interaction with iNOS has not been systematically explored. This study is the first to comprehensively evaluate these secondary metabolites against iNOS using an in silico approach combing biological activity prediction, physicochemical and pharmacokinetic profiling, and molecular docking simulations. From 53 screened metabolites, nine compounds met drug-likeness and pharmacokinetic criteria and were further analyzed through molecular docking.  Ellagic Acid, (+)-Spathulenol, α-Cadinene, and τ-Muurolol showed substantial binding affinities at the iNOS active site, which suggests that they could be good anti-inflammatory agents. These results establish a basis for subsequent in vitro and in vivo validation aimed at creating safe, natural iNOS inhibitors as herbal-based for the alternative anti-inflammatory medicines.
Enhancing students Agustina, Tri Wahyu; Solikha, Maratus; Rizky, Umar; Yuliawati, Astri
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4777

Abstract

This study aims to increase students
Quantitative analysis of nuclear morphological alterations in oral mucosal epithelial cells exposed to electromagnetic radiation from mobile devices Pakpahan, Enni Halimatussadiyah; Hartono, Adi
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4763

Abstract

Electromagnetic wave (EMW) radiation from mobile devices has raised public health concerns due to its potential biological effects on rapidly regenerating tissues such as the oral mucosa. This study quantitatively analyzed nuclear morphological alterations in oral mucosal epithelial cells following controlled EMW exposure. A quasi-experimental pretest–posttest control group design was conducted with 30 healthy participants aged 18–25 years, randomly assigned to control (n = 15) and treatment (n = 15) groups. The treatment group was exposed to EMW at 1800 MHz for 60 minutes per day over 14 consecutive days, using an active smartphone placed 1 cm from the right cheek during a simulated voice-call condition. The control group received no direct exposure. Buccal epithelial cells were collected via oral swab, fixed in Carnoy’s solution, stained with hematoxylin–eosin, and observed under a light microscope at 400× magnification. Quantitative assessment focused on three types of nuclear abnormalities: pyknosis, karyorrhexis, and karyolysis. The Independent Samples t-test showed a significantly higher mean number of nuclear abnormalities in the exposed group than in the control group (12.47 ± 3.15 vs. 4.86 ± 1.92 cells/field; p < 0.001). Pyknosis was the most frequent alteration (58.3%), followed by karyorrhexis (26.7%) and karyolysis (15.0%). All procedures were approved by the institutional ethics committee. In conclusion, short-term exposure to 1800 MHz EMW from mobile devices under controlled conditions can induce measurable degenerative nuclear changes in oral epithelial cells, suggesting cellular susceptibility to non-ionizing radiation at the cytomorphological level.
Morphology and habitat suitability of Rhacophorus barisani (Harvey, Pemberton & Smith, 2002) in Karo Regency, North Sumatra Tambunan, Muhammad Iqbal H.; Ritonga, Yusran Efendi
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4495

Abstract

Research on Rhacophorus barisani (Harvey, Pemberton & Smith, 2002) has been limited since its formal description in 2002. Since 2017, the IUCN has classified the Barisan Tree Frog as Least Concern, and this species is endemic to the mountain forests of the Bukit Barisan Mountains in Sumatra. Published records are limited to distribution notes and photographs, with no previous reports from Karo Regency, North Sumatra. This study was conducted between September and November 2023 in the Bukit Barisan Grand Forest Park (Tahura) and Gunung Sibuatan Protected Forest. The methods used were Visual Encounter Survey-Night Stream (VES-NS) and purposive sampling. This species was described morphologically and morphometrically, and habitat suitability data were recorded based on abiotic parameters. R. barisani was found in flowing water habitats and still water pools within the forest. R. barisani has three distinct dorsal color patterns: a light brown base color with a yellowish-brown color on the back that does not reach the sides of the body; a uniform yellowish-brown base color with yellow edges and a distinct dorsal pattern and black bands on the limbs; a green base color with scattered brown or black protuberances on the back, and a snout-vent length ranging from 41.9 to 65.7 mm. The habitat suitability of this species occupies riparian and lotic areas. Critically, we confirm that this species is the first record in Karo Regency, representing a new local discovery.
Vegetation diversity of mangrove forest in Percut Village, Percut Sei Tuan District, North Sumatra Azwar, Edi; Feviani, Yusri; Haloho, Bertaulina; Tarigan, Mhd. Rafi'i Ma'arif
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4808

Abstract

This study aimed to analyze the vegetation diversity of mangrove forest in Percut Village, Percut Sei Tuan District, North Sumatra. A quantitative descriptive method with a field survey approach was employed, using purposively selected stations representing natural mangroves (Station I), mangroves adjacent to fishponds (Station II), and mangroves near an ecotourism area (Station III). Data were collected using 5 × 5 m² plots with 10 m spacing, including species identification, abundance measurements, and physicochemical parameter assessments (temperature, salinity, soil pH, and substrate texture). Vegetation characteristics were analyzed using the Shannon–Wiener diversity index (H’), evenness index (E), and Simpson’s dominance index (D). Results showed that environmental parameters across stations remained within the optimal range for mangrove growth (temperature 18.6–19.7°C, salinity 16.5–19‰, and pH 7–8.5), though variations indicated differing levels of anthropogenic pressure. Eight true mangrove species were identified: Sonneratia alba, Bruguiera cylindrica, Nypa fruticans, Acanthus ilicifolius, Rhizophora apiculata, Avicennia marina, Rhizophora mucronata, and Xylocarpus granatum. Vegetation analysis revealed a strong dominance of Rhizophora apiculata and Bruguiera cylindrica, while Xylocarpus granatum and Rhizophora mucronata occurred in limited numbers. The Shannon–Wiener diversity index (H’ = 0.8442) indicated low diversity and uneven species distribution, suggesting community instability linked to anthropogenic disturbances. These findings highlight the ecological impacts of land conversion and tourism activities on mangrove ecosystems and emphasize the need for conservation and rehabilitation efforts to maintain biodiversity and ecosystem services in the Percut Mangrove Forest.
Community perception and participation in Silo Laut regarding mangrove management and conservation in Asahan Regency Rumondang, Rumondang; Harsono, Tri; Batubara, Juliwati Putri; Laila, Khairani; Ansoruddin, Ansoruddin; Astuti, Dewi; Tambunan, Syafrida Br; Fransiska, Adelia; Putri, Zeini Ardianti
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 2 (2025): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i2.4691

Abstract

The mangrove ecosystem plays a vital role in maintaining the balance of coastal environments; however, pressures from human activities such as land conversion and overexploitation have led to a decline in its quality and area. This condition necessitates management and conservation efforts that actively involve coastal communities as key stakeholders. This study aims to determine the perception and participation of coastal communities in Silo Laut District, Asahan Regency, regarding the management and conservation of mangrove ecosystems. The research employed a survey method by distributing questionnaires to 276 respondents across three villages: Bangun Sari, Silo Baru, and Lubuk Palas. Data were collected through field observations, structured interviews, and questionnaires containing statements based on the Likert scale. The data were analyzed using the SMART method and Likert scale, and statistically tested with ANOVA analysis. The results showed that most community members have a good perception of the ecological functions of mangrove forests; however, the level of participation and active engagement in conservation activities remains relatively low. The ANOVA analysis revealed that education level, length of residence, and income significantly influence community perception and participation in mangrove ecosystem management, while age and occupation do not show a significant effect. This study provides empirical evidence that community-based socio-economic empowerment programs are a key strategy to support sustainable mangrove conservation in the coastal areas of North Sumatra.

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