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Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi
Published by Universitas Islam Negeri Sumatera Utara
ISSN : 26213702     EISSN : 26217538     DOI : 10.30821
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 152 Documents
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Petrosavia sakuraii (Makino) J.J.Sm. ex Steenis on Mount Sibuatan, North Sumatra Ritonga, Yusran Efendi; Tambunan, Muhammad Iqbal H
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 7, No 2 (2024): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v7i2.2483

Abstract

This study presents new findings on Petrosavia sakuraii (Makino) J.J.Sm. ex Steenis, collected from Mount Sibuatan, North Sumatra. P. sakuraii (Makino) J.J.Sm. ex Steenis is a rare mycoheterotrophic plant in Indonesia, and its existance has not been scientifically reported until now. The research was conducted from July to November 2022 in the Gunung Sibuatan Protected Forest, Karo, North Sumatra. Data collection was carried out through exploratory methods. The samples found were documented and identified using various references. The results of the study include descriptions, illustrations, flowering and fruiting periods, habitat, distribution, and conservation status of P. sakuraii (Makino) J.J.Sm. ex Steenis. According to the IUCN Red List, this plant is classified as Data Deficient (DD) due to limited global data. Therefore, this finding contributes to the knowledge of the distribution of P. sakuraii (Makino) J.J.Sm. ex Steenis. This report marks the first recorded presence of this species in North Sumatra, specifically on Mount Sibuatan.
Species composition and abundance of mangrove gastropods in Desa Sutera, Kayong Utara, West Kalimantan Safitri, Ikha; Ayzah, Dzul Khoidzah All; Nurdiansyah, Syarif Irwan; Nguyen, Duc-Hung
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 7, No 2 (2024): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v7i2.3950

Abstract

The mangrove ecosystem in Desa Sutera, Kayong Utara Regency, West Kalimantan, Indonesia, serves as a habitat for various species of gastropods, many of which hold significant ecological and economic value. Data collection on gastropod species in this region is vital for establishing a baseline understanding of coastal and marine biodiversity, which is essential for the development of integrated and sustainable coastal management strategies. This study aimed to assess the species composition and abundance of mangrove gastropods in Desa Sutera. Sampling was conducted in May 2024 during low tide across four stations using quadrat transects. Thirteen species of mangrove gastropods were identified, representing nine genera and seven families, including Chicoreus capucinus, Clypeomorus bifasciata, Cassidula nucleus, Littoraria carinifera, L. melanostoma, L. scabra, Onchidium sp., Nerita balteata, Neripteron violaceum, Pirenella alata, P. cingulata, Cerithidea quoyii, and C. obtusa. Among these, P. cingulata and L. carinifera were the most abundant, whereas L. melanostoma and C. obtusa were the least prevalent. The gastropods were found inhabiting sandy and rocky substrates, as well as attached to the roots, trunks, and leaves of mangrove trees. This finding offers essential insights into the biodiversity and community structure of mangrove gastropods in the region, forming a basis for conservation and management strategies to protect this vital ecosystem.
Exploration of characteristics of trichomes, chlorophyl content, and lead content in Fabaceae leaves in the Malabar Urban Forest, Malang Latifa, Roimil; Hadi, Samsun; Nurrohman, Endrik
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 7, No 2 (2024): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v7i2.3762

Abstract

This research aimed to analyze the characteristics of trichomes, chlorophyll and lead content of various leaves in the Fabaceae family in the Malabar Urban Forest, Malang. The research was carried out using direct exploration methods (for sampling the leaves of each plant), and laboratory observations. The research will start from July 2023 to May 2024. The research was carried out in the Malabar Urban Forest, Malang, and the Biology Laboratory of UMM. Observation of anatomical structures using a binocular microscope, leaf chlorophyll content using a spectrophotometer, and lead content using the AAS method. The results of the research show that [1] there are variations in the characteristics of leaf trichomes in terms of type and other characteristics and there are variations in the number or average of trichomes; [2] Chlorophyll content in various types of leaves of the Fabaceae family in the Malabar Urban Forest. The highest chlorophyll a and b content is found in the Samanea saman plant and the lowest in Erythrina crista-galli; and [3] leaf lead content shows variation. The highest lead content is in the leaves of the Samanea saman plant and the lowest is Gliricidia sepium and Senna siamea. This research provides implications as baseline data for monitoring changes in trichome characteristics, chlorophyll content and lead in the leaves of Fabaceae family plants in the Malabar Urban Forest in the future as indicators of environmental dynamics. Information regarding variations in Fabaceae leaf characteristics can be used to develop bioindicators of environmental pollution, especially related to heavy metal pollution, as well as provide input for the management and conservation of urban forests.
Production of tempe koro benguk using local starter and its implementation as a biotechnology module for high school students Septiani, Aulia; Mamangkey, Jendri; Adinugraha, Fajar; Mendes, Lucas William
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 7, No 2 (2024): December
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v7i2.3748

Abstract

The Kurikulum Merdeka emphasizes integrating local wisdom into education to develop students’ soft skills and character. Traditional foods like koro benguk tempe (TKB), a fermented product made from Mucuna pruriens (L.) DC. from Dimoro Village, Central Java, offer an excellent avenue for such integration in biotechnology education. A survey at Yadika 4 High School revealed a lack of local wisdom-based practical guides, demanding the need for resources that could enhance student engagement in biotechnology knowledge. This study developed practical guidelines for TKB production, focusing on its organoleptic properties, integration feasibility, and feedback from teachers and students. Laboratory experiments and module development were combined to collect data. Organoleptic tests identified 0.3% starter concentration as optimal, producing tempe with a white color, soft texture, and pleasant taste. The practical guidelines achieved a validation score of 89% from language, material, and media experts, while teachers rated them at 90%, and students responded positively with a 73% score. Further research is needed to refine the fermentation process of TKB beyond 36 hours and analyze its pH content. Expanding the study to include other fermented local products could further enhance biotechnology learning. Integrating local wisdom through practical guides not only promotes cultural preservation but also improves students' memory, manipulative skills, creativity, communication, and responsibility.
Insect pollinators on Ananas comosus Linn. in pineapple cultivation hub at Tangkit Baru Village, Jambi Province: A preliminary study Ratna, Yuni; Ristyadi, Dwi; Arifah, Windyna
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.3732

Abstract

The flowers of Ananas comosus, pineapple, are generally self-sterile which means seeds will only develop if cross-pollination occurs. Therefore, insect pollinators play a crucial role in fostering and preserving genetic diversity in pineapple plants. Our research aimed as a preliminary study to gain insight into the key pollinators that play a significant role in the pollination of A. comosus in Tangkit Baru village, a prominent pineapple cultivation hub in Jambi Province. Sampling was done in 20 sampling points, five times at five-day intervals. Sampling was carried out using yellow sticky traps, sweep net, and direct observation. All the collected insects were identified to species level, then species diversity and evenness were assessed using Shannon’s diversity index (H’) and Pielou’s evenness index (J’). A total of 1167 individuals, representing 31 distinct species of insect pollinators belonging to 14 families were found in our sampling sites. We found four dominant species that could potentially serve as the key pollinators of A. comosus in our sampling site, i.e. Dolichoderus thoracicus, Pygophora enigma, Pygophora respondens, and Anoplolepis gracilipes. The diversity and evenness of insect pollinators in the sampling site were at a moderate level, with Shannon’s diversity index (H’) value of 2.0785 and pielou’s evenness index (J’) value of 0.6053. Habitat enhancement measures are needed to preserve and improve the existing pollinator diversity and evenness in the agroecosystem.
Formulation of Jakaba fertilizer from banana peel, pineapple peel, tea dregs for enhanced pak choi (Brassica rapa subsp. Chinensis) growth Muttaqin, Salwa Zainum; Mamangkey, Jendri; Silalahi, Marina; Mendes, Lucas William
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4470

Abstract

Excessive use of chemical fertilizers can lead to environmental degradation and increase the financial burden on farmers. As a sustainable alternative, liquid organic fertilizer (LOF) derived from Jakaba (also known as jamur keberuntungan abadi) was fermented using common organic wastes. This study aimed to evaluate the effects of different organic waste substrates on the characteristics of Jakaba-based LOF (JLOF) and the growth performance of pak choi (Brassica rapa subsp. Chinensis). A completely randomized design (CRD) was used, consisting of nine treatments with three replications each. The concentration variations of banana peel waste, pineapple peel, and tea grounds used in fermentation include 100 g, 200 g, and 300 g. Observations were conducted in three time intervals on days 0, 14, and 30. Parameters measured included the physical and biological characteristics of the LOF (i.e., pH and fungal growth), as well as plant height, leaf number, and fresh weight of pak choi. The data obtained were analyzed statistically using ANOVA analysis. The results showed that tea dregs provided the most favorable conditions for Jakaba growth and maintained the most stable pH. In contrast, pineapple and banana peels inhibited fungal growth, likely due to the presence of inhibitory compounds such as tannins and bromelain. Field application on pak choi indicated that JLOF derived from tea dregs promoted the best plant growth among all groups. These findings suggest that Jakaba-based LOF formulated with tea dregs holds promise as an effective organic fertilizer and may be further developed for commercial use.
The effect of guided inquiry with differentiated learning to enhance students' critical thinking skills Ningsih, Widia; Sipayung, Dwi Yanti Cornelia; Simatupang, Halim
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4196

Abstract

This study was designed to determine the effect of guided inquiry model integrated with differentiated learning on students' critical thinking skills on respiratory system material in class VII junior high school. The background of this research is based on the low critical thinking skills of students in Indonesia, as well as the need for a learning approach that is able to respond to the diversity of learning styles, interests, and readiness of students in the classroom. The guided inquiry with differentiated learning model, employs a method of instruction that encourages students to solve problems through the application of analytical and critical thinking skills. The present study employs a quasi-experimental design, specifically pretest-postest control group design. The present study utilizes a critical thinking ability test instrument according to Ennis, comprising 10 essay questions. Hypothesis testing was carried out with a significance level of 0.05 with the help of SPSS version 26. The research results demonstrated a significant influence of the guided inquiry learning mode with differentiated learning on students' critical thinking abilities, as measured by the Mann-Whitney U test with Asymp scores. Sig (2-tailed) 0.000, indicating a significance value <0.05. In conclusion, guided inquiry with differentiated learning is effective to enhance students' critical thinking skills and can be an alternative adaptive and responsive science learning strategy in the Merdeka Curriculum.
The potential of pineapple peel waste fermentation as antibacterial and antioxidant agents Mawli, Rizka Efi; Putri, Devi Anggraini; Dewi, Maharani Putri; Alfaini, Galuh Nuri; Febriani, Afaf; Anggraini, Dwi Aprilia; Sutanto, Andri Kurniawan
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.3887

Abstract

The fermentation of pineapple peel waste can yield a product in bioactive compounds, including an eco-enzyme that serves as both an antibacterial and antioxidant agent. By utilizing the fermentation process, the extraction of phenolic compounds is enhanced through the action of degrading enzymes produced by microorganisms. This study aims to explore the potential of fermenting pineapple peel waste from with varying fermentation durations of one month (designated as EE-1) and three months (designated as EE-3) to assess their antibacterial and antioxidant properties. The method research is purely experimental methodology with several testing phases: the preparation of the eco-enzyme, a disc diffusion test for antibacterial activity, the determination of the minimum inhibitory concentration (MIC) using the resazurin microtiter assay (REMA), and the assessment of antioxidant activity through the DPPH test. The result showed that the eco-enzyme fermentation has a dark brown color for EE-1 and a light brown color for EE-3, with a pH range of 3 to 3.2 and a sour pineapple aroma. EE-3 exhibited the highest antibacterial and antioxidant activity. It demonstrated strong inhibition against Propionibacterium acnes at concentrations of 100and 102, with MIC values of 3.13 mg/mL for Staphylococcus aureus, 12.5 mg/mL for Staphylococcus epidermidis, and 6.25 mg/mL for P. acnes. The minimum bactericidal concentration (MBC) was found to be 25 mg/mL for all tested bacteria, while the antioxidant activity of EE-3 was indicated by an IC50 value of 1.95 mg/mL.
The superiority of Zymomonas mobilis in producing bioethanol from Coffea arabica peel using cellulolytic enzyme treatment Widyaningrum, Trianik; Utami, Listiatie Budi
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4482

Abstract

As petroleum supplies are being depleted, there is a growing need for bioethanol as an alternative energy source. With the aid of crude cellulose enzymes from Bacillus subtilis IFO 13719, coffee arabica peels can be used as one of the waste materials to manufacture bioethanol. Microorganisms that can convert sugar into bioethanol, specifically Saccharomyces cerevisiae and Zymomonas mobilis, are also necessary for the synthesis of bioethanol. The purpose of this study is to demonstrate Zymomonas mobilis's superiority in generating bioethanol from fermented Arabica coffee peels treated with the crude cellulose enzyme B. subtilis. The concentrations of the B. subtilis crude cellulase enzyme at 0%, 2.5%, 5%, 7.5%, 10%, 12.5%, 15%, and 17.5% were all independent variables in the fully randomized experimental design. The amount of sugar and ethanol is the dependent variable. The DNS method was used to measure the sugar concentration, and an alcohol meter was used to determine the ethanol content. Data analysis using the SPSS 16 software to examine DMRT and one-way ANOVA. The results showed that the maximum sugar content (1.0 g/mL) was obtained with the cellulase enzyme B.subtilis 2.5 % and the highest ethanol content (4.46 %) was obtained with the crude enzyme B. subtilis concentration 10% and fermented using Z. mobilis.
Environmental literacy profile of Muhammadiyah junior high school students in Malang city: Intervention without having to consider factors! Husamah, H.; Rahardjanto, Abdulkadir; Hadi, Samsun; Lestari, Nurdiyah; Permana, Tutut Indria
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4262

Abstract

Students of Muhammadiyah Junior High Schools and Muhammadiyah Islamic Junior High Schools need to have environmental literacy to practice environmentally friendly attitudes, become nature conservationists, and fulfill the school's role in serving education and the environment. However, there has been no mapping of EL profiles in Muhammadiyah Junior High Schools and Muhammadiyah Islamic Junior High Schools in Malang City. This study aims to analyze the EL profile of Muhammadiyah Junior High Schools and Muhammadiyah Islamic Junior High Schools in Malang City. Using a cross-sectional survey approach, EL data from 412 students in grades VII, VII, and IX in six Muhammadiyah schools were collected and analyzed based on gender, class, and parental education. The results showed that there were no significant differences in EL between male and female students, as well as between classes, and parental education. This study concludes that interventions or policies to improve environmental literacy can be applied universally, regardless of gender, grade level, or parental education.

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