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Jurnal Biolokus: Jurnal Penelitian Pendidikan Biologi dan Biologi
Published by Universitas Islam Negeri Sumatera Utara
ISSN : 26213702     EISSN : 26217538     DOI : 10.30821
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 139 Documents
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Scientific literacy-based encyclopedia on the antiaging properties of acehnese spices: A research and development approach Fiyantara, Aljun; Diningrat, Diky Setya; Hasairin, Ashar; Hartono, Adi; Salleh, Rabeta Mohd; Sari, Ayu Nirmala
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.3928

Abstract

The aim of this research is to produce an encyclopedia of Acehnese spice plants that serves as an anti-aging agent developed based on scientific literacy. The encyclopedia of Acehnese spice plants will be used as one of the learning media in the Ethnobotany course. The development procedure was carried out using the ADDIE method which includes analyzing student needs and characteristics, formulating learning objectives, formulating material items, preparing evaluation instruments, writing drafts of encyclopedia, expert validation, field trials, and printing production scripts. Ethnobotanical experts conducted expert validation. The results of the expert validation test stated that the encyclopedia of Acehnese spice plants with antiaging potential as a learning resource to improve student science literacy in ethnobotany courses followed the design and the material included in it was suitable for use as learning media. The field trial results showed that the science literacy-based encyclopedia was very attractive in design and effectively used as learning media.
Exploring the implementation of the merdeka curriculum in biology education: A qualitative study at an Indonesian upper secondary school Tarigan, Mhd. Rafi'i Ma'arif; Azis, Suhartini; Lase, Natalia Kristiani; Tarwiyani, Tarwiyani
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4490

Abstract

This study aims to describe the implementation of the Merdeka Curriculum in Biology learning at SMA Negeri 1 Sibolga and to analyze its supporting and inhibiting factors. The study was conducted in the odd semester of the 2024-2025 academic year using a descriptive qualitative approach. Data collection techniques included observation, interviews, and documentation. The results show that the implementation of the Merdeka Curriculum in Biology was carried out gradually. Teachers showed strong enthusiasm and initiative in understanding and applying curriculum principles such as differentiated learning and project-based learning. The school supported this effort by providing a Biology laboratory, digital learning resources, and internal training. However, several challenges emerged, including unequal teacher understanding of the curriculum, difficulties in preparing teaching materials aligned with the learning outcome, and limited practical training. Learning evaluation was conducted regularly through formative assessments and classroom observations. Routine reflection served as a crucial means of improving instruction. The study concludes that the success of curriculum implementation is strongly influenced by teacher readiness, school support, and active student engagement. Strengthening teacher capacity and inter-teacher collaboration is recommended as a strategic step to optimize curriculum implementation.
Characterization of marine bacteria isolates GaN 2 and RaN 2 from Papuma Coastal, Jember Wathon, Syubbanul; Senjarini, Kartika; Oktarianti, Rike; Hasanah, Fitrianti Milhatul; Oktavia, Melinda Dwi Putri; Puspitasari, Endah; Labes, Antje
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4289

Abstract

The exploration of secondary metabolites for various human needs has now extended to aquatic environments. The fluctuating and dynamic nature of aquatic environments can stimulate marine bacteria to adapt by producing secondary metabolites. The diversity of marine bacteria has become increasingly promising as a target for obtaining various potential secondary metabolites. This study aims to isolate potential marine bacteria that produce secondary metabolites from the Papuma Beach area in Jember. Marine bacterial characterization can be carried out through morphological and molecular approaches. In this study, bacterial isolates were collected from marine algae at aquatic sites. Bacteria were isolated from the algae using the spread plate and streak plate methods on nutrient agar dissolved in artificial seawater as the medium until single colonies were obtained. Morphological characterization of the single colonies was conducted by observing colony shape and color, as well as performing Gram staining. Molecular characterization of selected single colonies was performed by amplifying the 16S rDNA as a bacterial molecular marker. The results of this study showed that marine bacteria that grew well and remained stable on artificial media were successfully isolated from green and red algae samples, labeled GaN 2 and RaN 2, respectively. The GaN 2 bacterial isolate was identified as Fictibacillus barbaricus strain SQ5-4, while the RaN 2 bacterial isolate was identified as Bacillus altitudinis strain 41KF2bT.26. According to previous studies, Fictibacillus barbaricus is known to have potential as a bioremediation agent, while Bacillus altitudinis has potential applications as an antitumor, antimicrobial, and antioxidant-producing agent.
In vitro germination of lemon cui (Citrus microcarpa Bunge) Pagalla, Devi Bunga; Kandowangko, Novri Youla; Ahmad, Jusna; Febriyanti, Febriyanti
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.3952

Abstract

Lemon cui (Citrus microcarpa Bunge) is widely utilized by the people of Gorontalo as a cooking ingredient, traditional medicine, cleaning agent, and cosmetic ingredient. This diversity of uses indicates the potential for further development of lemon cui. The initial stage of development that can be undertaken is the mass propagation of high-quality lemon cui seedlings. This study aims to produce lemon cui seedlings using in vitro culture techniques through seed germination.The experimental design used in this study is a Completely Randomized Design (CRD) with four treatments, namely:  A: MS0 (without the addition of plant growth regulators and young coconut water); B: MS + 50 mL young coconut water + 0.5 ppm 2,4-D; C: MS + 50 mL young coconut water + 1 ppm 2,4-D; and D: MS + 50 mL young coconut water + 1.5 ppm 2,4-D. The culture medium used was Murashige and Skoog (MS) supplemented with young coconut water and 2,4-dichlorophenoxyacetic acid (2,4-D). The study's results showed that using young coconut water and 2,4-D significantly accelerated the germination of lemon cui seeds. The best result was observed in treatment D, where the citrus seeds exhibited a maximum germination rate of 100%. In addition, the combination of young coconut water and 2,4-D is capable of inducing callus formation during the germination process. The combination of 50 mL of young coconut water and 1.5 ppm 2,4-D is the most effective concentration for inducing germination of lemon cui seeds and callus formation.
Identification of quercetin compounds in cabbage (Brassica oleracea var. capitata L.) and mustard greens (Brassica juncea L.) from Malang Regency Permana, Fendy Hardian; Rakhma, Zulfaza A-Janah; Latifa, Roimil
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.3637

Abstract

Free radicals are chemical molecules with an unpaired electron in their outermost orbit. Free radicals are often referred to as Reactive Oxygen Species (ROS) which have reactive and labile properties so they tend to capture electrons from other molecules. Unhealthy lifestyles have a very high risk of increasing free radicals in the body. The imbalance of antioxidants with free radicals entering the body will cause oxidative stress. Consuming healthy foods, one of which is vegetables, is an effort that can be made to maintain a healthy body. The abundant production of cabbage and mustard greens in Malang Regency is expected to provide good benefits to help ward off excess free radicals. Quercetin compounds are one of the most common compounds found in plants and contain good antioxidants. The purpose of this study was to identify the content of quercetin compounds in cabbage (Brassica oleracea var. capitata L.) and mustard greens (Brassica juncea L.) in Malang Regency. The method used is the quercetin compound test, with measurement performed using a UV-Vis spectrophotometer to determine the levels of compounds in cabbage and mustard greens extracts. The results of the study showed that after adding HCl reagents and Mg powder to cabbage and mustard greens, a dark red color change occurred, indicating that the results were positive for quercetin compounds. Measurements using a UV-Vis spectrophotometer obtained quercetin compound levels in cabbage (Brassica oleracea var. capitata L.) of 358.34 ppm or 3.5% and mustard greens (Brassica juncea L.) of 360.87 ppm or 3.61%.
Bazzania genus of Marchantiophyta in the Batang Toru Forest, North Sumatra, Indonesia Damanik, Risjunardi; Pasaribu, Nursahara; Siregar, Etti Sartina; Syamsuardi, Syamsuardi
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4390

Abstract

This study aims to enhance the existing knowledge on the diversity of liverworts within the genus Bazzania in Indonesia, specifically in North Sumatra, where such research is relatively limited. North Sumatra offers a rich habitat for liverworts, particularly within the Batang Toru forest, which experiences extremely high rainfall, resulting in optimal humidity for liverwort growth, including Bazzania species. The goal of this study is to contribute to the literature regarding Bazzania found in the Batang Toru forest. Sample collection involved exploring the hiking trails of the Batang Toru Forest as part of an exploration survey. Bazzania specimens were collected directly from leaves and cut from bark, rocks, or soil using a knife. All collected Bazzania liverworts were placed in designated dry specimen envelopes. This research successfully identified 13 Bazzania species, three of which are new records for Sumatra: Bazzania parabidentula, Bazzania parisii, and Bazzania scalaris. The most frequently collected specimens were Bazzania caudistipula, Bazzania calcarata, and Bazzania densa. The presence of Bazzania in the Batang Toru forest indicates that the area remains in a natural state. Consequently, this research aimed to inventory Bazzania in the Batang Toru forest and to enhance the understanding of Indonesia's natural wealth, particularly concerning Marchantiophyta.
Specific primer design for microbiome profiling of the dengue vector Aedes aegypti based on 16S ribosomal DNA Ariesaka, Kiky Martha; Sishartami, Lintang Widya; Weningtyas, Anditri; Nuryady, Moh Mirza; Wiratama, Arif Ladika; Bakhtiar, Fannia Yosa
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.3975

Abstract

Identification of the microbiome in the dengue vector Aedes aegypti is crucial for developing effective vector control strategies. The 16S ribosomal DNA (rDNA) gene is frequently used as a genetic marker in microbiome analysis. This study aimed to design and validate specific primers to identify Serratia and Wolbachia in Aedes aegypti. The study employed Primer-BLAST using DNA sequence data from NCBI (accession numbers OR801069.1 for Serratia and MN046789.1 for Wolbachia). The designed primers included: for Serratia—Forward 5’-GTCGACTTTGATCCTGGCTCAG-3’ and Reverse 5’-TCAGCCTGTTTCCAATGACC-3’; and for Wolbachia—Forward 5’-TGATGAAGTTAGCTTGCTAACG-3’ and Reverse 5’-ACGGCTGAGTGAACGGGTG-3’. Validation was conducted through PCR, followed by visualization of the amplification products using 1.5% agarose gel electrophoresis. PCR amplification revealed distinct DNA bands of 766 bp for Serratia and 556 bp for Wolbachia, confirming primer specificity. The Sanger sequencing results were validated using BLAST, showing high alignment with reference strains in the GenBank database. The specific primers based on the 16S rDNA gene are effective for detecting Serratia and Wolbachia in Aedes aegypti.
Knowledge ecoliteracy of junior high school students towards Toba Caldera UNESCO Global Geopark Rosdiana, Erika; Suyanti, Retno Dwi; Manurung, Binari
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.4469

Abstract

This study aimed to determine the level of knowledge ecoliteracy of junior high school students towards Toba Caldera UNESCO Global Geopark (TCUGGp) was conducted. The research respondent was 78 people, including 28 students from Toba regency and 25 students each from North Tapanuli and Samosir regencies. The research instrument comprised 30 questions and was developed from five indicators, namely factual issues of environmental problems, the impacts, the factors causing environmental problems, efforts that can be made to minimize the impact of environmental degradation, flora-fauna biodiversity and the name of geosites. Data analysis used descriptive and inferential methods. The research results indicated that the knowledge ecoliteracy of junior high school students remains relatively low (X̅ = 58.28). ANOVA test results showed a significant difference in students'knowledge ecoliteracy among the three regencies (F = 3.24; P = 0.04). Further findings revealed that junior high school students have limited understanding in the factors causing environmental problems, efforts to minimize the impacts of environmental degradation, and more specifically in the diversity of rare and endemic of flora and fauna, as well as in geosite names. This research finding recommends that IPA-biology learning in junior high schools especially at the topic biodiversity, interactions between living organisms and the environment, and environmental changes should be conducted by using more innovative learning models, as well as its media, learning resources, and assessments that refers to local social scientific issues so that ensuring the creation of meaningful learning.
The effect of genitri (Elaeocarpus ganitrus) in leukocytes profile of wistar rats as a prospective immunomodulatory agent Primiani, Cicilia Novi; Pujiati, Pujiati; Bhagawan, Weka Sidha; Waris, Waris; Krisnamurti, Gabriella Chandrakirana
Jurnal Biolokus : Jurnal Penelitian Pendidikan Biologi dan Biologi Vol 8, No 1 (2025): June
Publisher : Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/biolokus.v8i1.3987

Abstract

Immunomodulator is an agent to activate and modulate the immune response. It is one of the approaches to prevent chronic disease and maintain homeostasis. Recently,  plant phytochemicals have been developed as prospective immunomodulatory agents. Genitri (Elaeocarpus ganitrus), a local plant cultivated in Indonesia, exhibited various biological properties and potential as an immunomodulatory agent. This study aims to identify the potency of genitri as immunomodulatory agent through leukocyte profiling. Genitri leaf (P1), seed (P2), and fruit (P3) were prepared as powder and administered to male wistar rats weighted 250-300 g twith six replications for 28 days. The total leukocytes were  counted and leukocytes were being  profiled following the treatment with genitri powder then  compared with control treatment (P0). The immunomodulatory activity of genitri was shown by the increased leukocytes count following treatment. Based on data from the treatment group (P2), genitri seeds were able to increase leukocytes by 10595.83 cells/µl. The highest total leukocytes were found in treatment P2 with total leukocytes 10595.83 cells/µl. Based on the leukocyte profile, lymphocytes and monocytes were found to be the highest in all treatments. Treatment with genitri was able to stimulate an increase in lymphocytes and monocytes. An increase in total leukocytes given genitri is an indication of an increased immune response.

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