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FaST : Jurnal Sains dan Teknologi
Published by Universitas Pelita Harapan
ISSN : -     EISSN : 25989596     DOI : -
Core Subject : Science, Social,
Computer Science & IT Environmental Science
Started in 2017, FaST publishes articles in the areas of science and technology, particularly in food technology, biology, electrical engineering, industrial engineering, mathematics and civil engineering. Articles are either reviews and/or research results.
Arjuna Subject : -
Articles 384 Documents
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PEMANFAATAN SEMANGKA DAN OKRA DALAM PEMBUATAN JELI STIK DENGAN VARIASI JENIS HIDROKOLOID [USE OF WATERMELON AND OKRA IN MAKING JELLY STICKS WITH VARIOUS TYPES OF HYDROCOLLOIDS] Alfian, Nathania Putri; Mastuti, Titri Siratantri
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 7 No. 1 (2023): NOVEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v7i2.7601

Abstract

Jelly is food product in semi-solid form derived from fruit or vegetable juices with the addition of gelling agent. Jelly sticks are easy to eat, carry around and loved by the public. In this research, watermelon and okra were used as jelly’s ingredients because these two ingredients have many health benefits The use of watermelon can also mask the unpleasant aroma and taste of okra. The type of hydrocolloid as gelling agent is an important factor in making jelly stick because it can influence the texture of jelly. The aim of this research was to determine the organoleptic, physic and chemical characteristics of the best jelly stick based on selected ratio of watermelon:okra and type of gelling agent. The treatment factors of jelly included ratio of water melon and okra (60:40, 50:50, 40:60) and the types of gelling agent (carrageenan, carrageenan:konjac, carrageenan:pectin). The best jelly stick was jelly made from a ratio of watermelon and okra 60:40 with a carrageenan:konjac as gelling agent. This jelly had the closest texture to jelly stick commercial with springiness of 0,95 ± 0,01 and cohesiveness of 0,70 ± 0,02. It had °Hue value 22,34 ± 0,97 as a red color, chewy texture, slightly preferred color and neutral overall preference from the panelist. The best jelly stick also had IC50 value of 82226,96 ± 6297,9 ppm, total phenolics of 0,157 ± 0,01 mg GAE/ml, total flavonoids of 0,022 ± 000 mg QE /ml and food fiber content 2,885g/100g.Bahasa Indonesia Abstract:Jeli merupakan produk pangan dalam bentuk semi padat yang berasal dari sari buah atau sayur dengan penambahan bahan pembentuk gel. Jeli stik mudah untuk dikonsumsi, dibawa bepergian serta banyak disukai masyarakat. Pada penelitian ini digunakan semangka dan okra sebagai bahan baku karena kedua bahan ini memiliki banyak manfaat kesehatan. Penggunaan buah semangka juga dapat menutupi aroma dan rasa khas okra yang kurang disukai. Jenis hidrokoloid sebagai gelling agent merupakan faktor penting dalam pembuatan jeli karena mempengaruhi teksturnya. Tujuan penelitian ini adalah menentukan karakteristik organoleptik, fisik, kimia jeli stik terbaik berdasarkan rasio semangka:okra dan jenis gelling agent terpilih. Faktor perlakuan pada jeli adalah rasio semangka dan okra (60:40, 50:50, 40:60) serta jenis gelling agent (karagenan, karagenan:konjak, dan karagenan:pektin). Jeli stik terbaik adalah jeli dengan rasio semangka dan okra 60:40 dengan jenis gelling agent karagenan:konjak. Jeli ini memiliki tekstur paling mendekati jeli stik komersial dengan springiness sebesaar 0,95 ± 0,01 dan cohesiveness sebesar 0,70 ± 0,02. Jeli ini memiliki nilai °Hue sebesar 22,34 ± 0,97 sebagai warna merah, tekstur kenyal, warna yang agak disukai dan kesukaan keseluruhan yang netral dari panelis. Jeli stik terbaik juga memiliki nilai IC50 sebesar 82226,96 ± 6297,9 ppm, total fenolik sebesar 0,157 ± 0,01 mg GAE/ml, total flavonoid sebesar 0,022 ± 000 mg QE /ml dan kadar serat pangan 2,885g/100g.
OPTIMASI DAN FORMULASI EKSTRAK ETANOL UMBI WORTEL (Daucus carota L.) SEBAGAI FACE SERUM SEBAGAI FACE SERUM [OPTIMIZATION AND FORMULATION OF CARROT ETHANOL EXTRACT (Daucus carota L.) AS A FACE SERUM] Karnelasatri, Karnelasatri; Trisina, Jessica; Purba, Rossa Maranatha
FaST - Jurnal Sains dan Teknologi (Journal of Science and Technology) Vol. 7 No. 1 (2023): NOVEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jstfast.v7i2.7604

Abstract

Carrot bulbs are known to contain secondary metabolite compounds that can act as antibacterials due to their activity, which suppresses the synthesis of nucleic acid, cytoplasmic membrane function, and energy metabolism in bacteria. This research was carried out using laboratory experimental methods through the determination of the plant, production of simplicia, extraction by ethanol (96% as solvent) with the maceration method, phytochemical screening, antibacterial assay, optimization of the base formula, formulation of carrot extract, physical evaluation of the products, and hedonic testing. The yield of 96% ethanol extract of the carrot bulbs was 53.29%, and the water content of the extract was 16.14%. Furthermore, the antibacterial activity of the extract (500.000 ppm) against Staphylococcus aureus showed an inhibitory zone of 5.1 ± 3.42 mm. The carrot extract serum formulas F1, F2, and F3 meet the organoleptic and homogeneity criteria; however, the F2 formula does not meet the pH and viscosity criteria. Based on the hedonic statistical test, no significant difference was found in each formula; nevertheless, based on the average value of the respondents, it was known that the F1 formula had the highest preference.Bahasa Indonesia Abstract:Umbi wortel dapat berperan sebagai antibakteri karena mengandung senyawa metabolit sekunder yang dapat menekan sintesis asam nukleat, fungsi membran sitoplasma, dan metabolisme energi pada bakteri. Penelitian ini dilakukan dengan metode eksperimental laboratorium yang dilakukan dengan determinasi tumbuhan, pembuatan simplisia serta ekstraksi dengan pelarut etanol 96% dengan metode maserasi, skrining fitokimia, uji antibakteri ekstrak, optimasi basis sediaan, formulasi sediaan, evaluasi fisik sediaan, dan uji hedonik sediaan. Rendemen ekstrak etanol 96% umbi wortel yang diperoleh adalah sebesar 53,29% dan kadar air ekstrak adalah 16,14%. Aktivitas antibakteri dari ekstrak (500.000 ppm) terhadap Staphylococcus aureus menunjukan zona hambat sebesar 5,9 ± 0,49 mm. Sediaan serum ekstrak umbi wortel F1, F2, dan F3 memenuhi syarat organolepik dan homogenitas, namun sediaan F2 tidak memenuhi syarat pH dan viskositas sediaan. Berdasarkan uji statistik hedonik sediaan, tidak ditemukan perbedaan signifikan pada tiap sediaan, namun berdasarkan nilai rata-rata responden diketahui bahwa sedian F1 menjadi preferensi tertinggi.
Formulasi dan Uji Iritasi Gel Antibakteri Dari Ekstrak Etanol Daun Belimbing Wuluh (Averrhoa bilimbi Linn) Terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa [Formulation and Irritation Test of Antibacterial Gel from Star Furit Leaves (Averrhoa bilimbi Linn.) Ethanol Extract Againts Bacteria Staphylococcus aureus and Pseudomonas aeruginosa] Sutriningsih Sutriningsih; Zuraida Sagala; Marhamah Marhamah
FaST : Jurnal Sains dan Teknologi Vol. 2 No. 1 (2018): MAY
Publisher : Universitas Pelita Harapan

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Abstract

Starfruit (Averrhoa bilimbi Linn.) is containing several chemical contents including tannins, alkaloids, flavonoids, polyphenols and saponins, which can be efficacious as an antibacterial. The purpose of this study is determine stability of  gel and the effect of gel formulation ethanol extracted from leaves starfruit (Averrhoa bilimbi Linn.) against the antibacterial activity of Staphylococcus aureus and Pseudomonas aeruginosa. This is an experimental laboratory research where the formulation of ethanol extracted from the leaves of starfruit (Averrhoa bilimbi Linn.) which  obtained from the process of maceration with 96% of ethanol solvent formulated in a gel dosage form. Gel formulations of the ethanol extracted from leaves starfruit was made with various concentration of the extract including 35%, 50% and 65% with hidroksi propil metil cellulose ( HPMC) as the base. We used without-extract as the negative control and  0.1% of gentamicin ointment as the positive control. The result gel from antibacterial activity was then processed using the agar diffusion method .The use of a gel formulation  ethanol extracted from leaves starfruit affected the antibacterial activity of Staphylococcus aureus and Pseudomonas aeruginosa . The results showed that the ethanol gel extracted from leaves starfruit (Averrhoa bilimbi Linn.) had the best antibacterial activity, indicated by 65% gel concentration containing the ethanol extracted from leaves starfruit being able to inhibit the growth of Staphylococcus aureus that for 28.62 mm with greater barrier diameter and Pseudomonas aeruginosa for 19 mm. Irritation test to 20 female with age 20 - 30 years old man and woman with put it on the back of hands for 2 hours in 3 days and the result is no irritation indication happend . Gel stability was had test for 4 weeks and nothing change happend 
Kajian Prediksi 3-Dimensi Biomarker Kanker Payudara Dari Jalur Ekspresi LincRNA-ROR/MIR-145/ARF6 [3D Prediction of Breast Cancer Biomarker from The Expression Pathway of LincRNA-ROR/MIR-145/ARF6] Arli Aditya Parikesit; Dito Anurogo
FaST : Jurnal Sains dan Teknologi Vol. 2 No. 1 (2018): MAY
Publisher : Universitas Pelita Harapan

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Abstract

According to WHO, breast cancer is one of the main causes of mortality in women. To overcome this malady, molecular biomedical research is carried out intensively. Anomalies in the lincRNA-RoR/miR-145/ARF6 expression pathway were found to play a very important role in breast cancer, especially in the type of Triple-Negative Breast Cancer (TNBC), which is the most dangerous variant of the deadly disease. Bioinformatics research has found the existence of non-coding RNA (ncRNA) in these expression pathways, whose interactions are worth studying with 3-dimensional prediction methods. The 3-D prediction method for biomolecules has been widely developed and has been successfully applied to DNA and protein. However, for the structure of RNA, it has just been developed, due to its low stability and very high dynamics on the biomolecule. Our aim is to apply the latest computational method for predicting the 3-dimensional structure of ncRNA, which can be applied as key information in biomedical application research. Extrapolation of kinetics and thermodynamic indicators of ncRNA ultimately yields the siRNA Linc-ROR 3-Dimensional structure and siRNA mRNA-ARF6, each having 13 and 8 hydrogen bonds. The existence of these hydrogen bonds is very important in maintaining the stability of the compounds and shows its efficacy as drug candidates. It is expected that preliminary information from the predicted 3-dimensional structure of ncRNA is useful for optimization of laboratory experiments in the field of crystallographic biomolecules.
Karakterisasi Protein Sorgum dan Upaya Peningkatan Jejaring Protein Sorgum Dengan Penambahan Glukosa-Oksidase [Characterization of Sorghum Protein and Improvement of Sorghum Protein Net-Working with Addition of Glucose-oxidase] Endah Wulandari
FaST : Jurnal Sains dan Teknologi Vol. 2 No. 1 (2018): MAY
Publisher : Universitas Pelita Harapan

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Abstract

Sorghum (Sorghum bicolor (L.) Moench) contains about 10% protein so it can act as a source of protein. Kafirin is a protein in sorghum distinguished on α, β, and γ-kafirin based on molecular weight differences, ease of extracting, and molecular structure. Characteristics of the sorghum protein and the ability of glucose-oxidase will form a protein network naturally between proteins or peptides resulting in polymerization. The objectives of the research were to fractionate protein of the flour of Zh-30 sorghum strain using five types of solvents. The protein fractions were analyzed for protein content and molecular weight by SDS-PAGE and evaluated for protein-network formation by glucose-oxidase, the result showed that flour protein of the sorghum Zh-30 strain consisted of five classes of proteins with a ratio of respectively albumin, globuline, kafirin 1, gluteline and kafirin 2 or cross-linked kafirin 11:11:42:7:29. Kafirin 1 content was 36.01%, kafirin 2 24.79%, gluteline 22.15%, globuline 12.81% and albumin 4.24% of total protein. Molecular weight of each protein fraction of the sorghum flour Zh-30 strain was respectively albumin 69.9 kDa, kafirin 1 which consisted α1-kafirin 22.4 kDa and α2-kafirin 23.5 kDa, globuline 121 kDa, gluteline 15.5 kDa and kafirin 2 which consisted of α1-kafirin 16.2 kDa, α2-kafirin 23.5 kDa, β-kafirin 24.6 kDa and γ-kafirin 53.6 kDa. Protein net-working was established when the glutelin fraction was treated with glucose-oxidase (MW 69 kDa from 15.5 kDa).
Kajian Pustaka : Pemanfaatan Mikroba Yang Berpotensi Sebagai Agen Bioremediasi Limbah Pewarna Tekstil [A Review : Utilization of Potential Microbes as Bioremediation Agent for Textile Dyes Waste water] Valerie Valerie; Reinhard Pinontoan
FaST : Jurnal Sains dan Teknologi Vol. 2 No. 1 (2018): MAY
Publisher : Universitas Pelita Harapan

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Abstract

The rapid expansion of textile industry in Indonesia raises substantial concerns with regard to the disposal of excess dyes into the environment. The deleterious consequences of such issue can be alleviated through wastewater management by combining physical, chemical and biological approach. Biological treatment through bioremediation needs to be thoroughly considered and developed as it offers favorable attributes by being sustainable, inexpensive and environmentally friendly. In Indonesia, the employment of bioremediation technique, to treat textile dye effluent, has been extensively applied through the use of activated sludge. Apparently, studies describing the potential of various indigenous microorganisms to degrade textile dyes have continuously been reported. This paper proposed practical methods to isolate and select for potential indigenous microbes as well as their employment as bioremediation agent on dye contamination. Selections can be enhanced through the incorporation of bioinformatics analysis on the approach of microbiology. Appropriate systems are required to be carefully designed to optimized application on-site.
Analisis Resiko Gempa Bendungan Leuwikeris, Provinsi Jawa Barat [Seismic Hazard Analysis Leuwikeris Dam, West Java] Fioliza Ariyandi; Muhammad Riza Harahap
FaST : Jurnal Sains dan Teknologi Vol. 2 No. 1 (2018): MAY
Publisher : Universitas Pelita Harapan

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Abstract

One of the dams in West Java is Leuwikeris Dam is being planned. It required an assessment in the form of seismic hazard analysis on Leuwikeris Dam. In this paper seismic hazard analysis was performed using probabilistic and deterministic methods. Where seismic events in the repeat periods of 145, 475 and 10000 years are considered in seismic hazard analysis by probabilistic methods. While the spectra target selection is based on CMS (Conditional Mean Spectrum). The results of this study show the design criteria for seismic risk Leuwikeris Dam on condition Operating Basis Earthquake (OBE) and Safety Evaluation Earthquake (SEE) in the form of acceleration time history. In short spectra t = 0.2 seconds value peak ground accelerations (PGA), respectively for 0,23g, 0,30g and 0,54g, while in the long spectra period t = 1.0 seconds value of peak ground accelerations (PGA) respectively of 0.16g, 0.19g and 0.26g, which will be used as input in conducting a dynamic analysis of Leuwikeris Dam.
Pemanfaatan Buah Leunca (Solanum nigrum L.) Dalam Pembuatan Minuman Sari Buah (Utilization of Black Nightshade (Solanum nigrum L.) in the Making of Fruit Juice) Yuniwaty Halim; Diana Effendi; Caecilia C. Nurwitri
FaST : Jurnal Sains dan Teknologi Vol. 2 No. 1 (2018): MAY
Publisher : Universitas Pelita Harapan

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Abstract

Black nightshade (Solanum nigrum L.) fruit contains many antioxidant compounds, however, utilization of black nightshade as food or beverage product has not been much studied. The research was aimed to determine sugar concentration and acid source used in black nightshade juice making to obtain a product that is acceptable for the panelists and to observe the black nightshade juice quality changes during storage. Sugar concentration used were 10%, 12.5%, and 15%. Acids used were from citric acid, belimbing wuluh (Averrhoa bilimbi) and lime (Citrus aurantifolia). It was found that the selected formulation was black nightshade juice added with 15% of sugar concentration and acid added from belimbing wuluh (Averrhoa bilimbi). This formula has acceptance value of 4.41± 1.357  and the IC50 value of 2118.0582 ± 96.3983 mg/L. Storage was done for 6 weeks in refrigerated temperature. During storage, quality of black nightshade juice decreased after 2 weeks, observed from decrease in its antioxidant activity, total phenolic and flavonoid compound.  
KARAKTERISASI FISIKO KIMIA DAN INHIBISI α-GLUKOSIDASE BERAS ANALOG DARI BUAH Rhizophora mucronata [CHARACTERIZATION OF PHYSICO-CHEMICAL AND α-GLUKOSIDASE INHIBITION OF ANALOG RICE FROM Rhizophora mucronata FRUIT] Hardoko Hardoko; Devy Alfiana; Yunita Eka Puspitasari
FaST : Jurnal Sains dan Teknologi Vol. 2 No. 2 (2018): NOVEMBER
Publisher : Universitas Pelita Harapan

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Abstract

ABSTRAKPenelitian ini untuk mengetahui karakteristik fisiko kimia dan inhibisi α-glukosidase  beras analog dari tepung buah R. mucronata, tepung singkong, dan tepung E. cottonii. Penelitian ini dilaksanakan pada bulan April - Oktober 2016. Metode eksperimen melalui perlakuan rasio tepung buah R. mucronata dengan Tepung singkong (60:40, 70:30, 80:20) dan penambahan tepung E. cottonii (0, 3, 5, 7%).  Pembuatan beras analog menggunakan extruder. Hasilnya menunjukkan bahwa perlakuan rasio tepung buah R. mucronata dengan tepung singkong dan penambahan tepung E. cottonii berpengaruh posistif nyata terhadap inhibisi Î±-glukosidase dan sifat fisiko kimia beras.  Semakin tinggi jumlah tepung  buah R. mucronata yang digunakan makin tinggi aktivitas inhibisi Î±-glukosidase dan menurunkan nilai IC50. Beras analog terbaik adalah yang terbuat dari tepung buah R. mucronata 60%, tepung singkong 40%, dan tepung rumput laut E. cottonii 5%. Beras ini mempunyai nilai IC50 33,42 ppm, kadar air 8,46%, kadar serat pangan 38,96%, berwarna merah (nilai oHue 44,86), cooking time 11,35 menit, volume pengembangan 135,09%, dan kadar pati 51,44%.
PEMBUATAN TELUR PINDANG DENGAN PENAMBAHAN DAUN JATI (Tectona grandis L. f.) DAN DAUN JAMBU BIJI (Psidium guajava L.) Ratna Handayani
FaST : Jurnal Sains dan Teknologi Vol. 2 No. 2 (2018): NOVEMBER
Publisher : Universitas Pelita Harapan

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Abstract

Pemindangan telur dapat menjadi upaya untuk meningkatkan daya simpan telur rebus dan keragaman makanan di Indonesia. Telur pindang merupakan produk pangan olahan tradisional  dengan kombinasi penggaraman dan perebusan dengan menggunakan bahan penyamakan protein. Telur pindang dibuat dengan lima rasio penambahan daun jati dan daun jambu biji (100: 0, 75:25, 50:50, 25:75, dan 0: 100). Sampel kemudian dianalisis fisikokimia yang meliputi kandungan tanin, warna, kadar protein, dan kadar lemak, jumlah lempeng total selama penyimpanan, dan uji organoleptik. Hasil penelitian menunjukkan bahwa perbedaan rasio daun jati dan daun jambu biji mempengaruhi total mikroorganisme dalam telur selama 24 jam penyimpanan, dan tingkat kecerahan telur pindang. Formulasi terbaik berdasarkan jumlah total lempeng dan uji organoleptik adalah telur pindang yang telah direbus dengan 2% daun yang terdiri dari 50: 50 daun jati: rasio daun jambu biji dan direndam pada air rebusan selama 12 jam. 

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