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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 334 Documents

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SINGLE-O-SHELTER HUNA (Cherax albertisi) AND REDCLAW (C. quadricarinatus) CULTURE Taufik Ahmad; Lilis Sofiarsih; Nuryadi Nuryadi; G. Apriyana
Indonesian Aquaculture Journal Vol 2, No 2 (2007): (December 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Many hatcheries successfully produced and sold cherax as ornamental crayfish. The attempt to culture cherax in earthen pond to produce consumable size yabbies facing the fact that cherax is a good hole digger and usually escapes through the hole in dyke. Single-o-shelter meant to provide shelter for every single spawner as well as hideout for the juvenile produced. The shelter for spawner was a 25 inches long and 2.0 inches diameter PVC pipe randomly spread on pond bottom. Aquatic weed (Vallisneria torta) grew in the shallow part of pond to provide hiding place for juvenile. The species stocked is huna and redclaw, each at density of 2 and 6 sets of spawner. One set of spawner consists of 3 males and 5 females weighing averagely around 20 g each. The experimental units are randomly selected to facilitate random block design in 2 rearing period as replicate. The pond dimension is 10x10 m, divide into 3 compartments i.e. feeding, ground, nursery ground and harvest ditch. Water depth at nursery ground was 30 cm and at the other compartments at 60 cm. Follow gravity force, the water in ponds flows at 50—100 L minute-1. Self-made diet distributed into pond twice a day to meet 3% daily feeding ration. Survival rate and specific growth rate of spawner as well as juvenile produced and number of gravid female checked at the end of each rearing period or every 3 months. After 6 months, average weight of redclaw and huna reaching 146.12 ± 34.47 g and 103.7 ± 29.83 g, respectively. Redclaw produced progeny of 5 size groups and huna produced only 2 groups. Respective to the species, average weight of the first offspring batch was 39.03 ± 5.33 and 26.83 ± 2.09 g. Redclaw at 2 sets of spawner and male grow faster than of 6 sets of spawner and female. No survival rate significant difference among ponds indicates that single–o-shelter technique provides sufficient shelter for spawner to grow and reproduce. Male monosex redclaw culture in earthen pond seems to be more promising than mixed-sex and female monosex culture for consumable size production of either huna or redclaw.

REMOTE SENSING AND GIS APPROACHES TOWARD SUSTAINABLE MANAGEMENT OF MARINE AQUACULTURE IN INDONESIA I Nyoman Radiarta
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The sustainable marine aquaculture (mariculture) has become increasingly important with the dramatic growth of the sector. To ensure long-term sustainability, planning is an important process that will stimulate and guide the evaluation of the sector. Many of the mariculture issues are entirely spatial in nature (e.g. siting, zoning), or have important spatial elements (potential mariculture areas, impact of mariculture, competition for space with other users). Satellite remote sensing integrated with geographic information systems (GIS) can play a major role in all geographic and spatial aspects of the development and management of mariculture. The potential capabilities of evolving GIS and remote sensing for the sustainability of mariculture provide a powerful tool for the efficient and cost effective management. These technologies are also useful for facilitating the decision making process in relation to mariculture. In this paper attempt to simply describe a number of ways in which GIS and remote sensing could be usefully employed as an aid to support sustainable mariculture development.

PATHOGENECITY OF GROUPER SLEEPY DISEASE IRIDOVIRUS (GSDIV: Megalocytivirus, FAMILY Iridoviridae) TO CORAL TROUT GROUPER Plectrophomus leopardus Ketut Mahardika; Ahmad Muzaki; Ketut Suwirya
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Grouper sleepy disease iridovirus (GSDIV), a member of the genus Megalocytivirus in the family Iridoviridae, has been known to cause large scale mortalities resulting in severe economic losses in grouper industries in south-east Asia including Indonesia. In this study, experimental infection of coral trout grouper Plectrophomus indicus with GSDIV was performed to evaluate the viral pathogenecity to this fish species. After virus exposure, the mortalities of coral trout grouper injected with primary and 10-1 dilution of spleen homogenates derived from tiger grouper Epinephelus fuscoguttatus were 100% and 90%, respectively. Histopathology revealed that moribund fish receiving GSDIV inoculum displayed massive formation of enlarged cells in the spleen and hematopoitic tissues. Under electron microscopy, the enlarged cells were observed as inclusion body bearing cells (IBCs) and necrotic cells allowing virus propagation within an intracytoplasmic virus assembly site (VAS). GSDIV virions were 167-200 nm in size. These findings confirmed that GSDIV has severe pathogenicity to coral trout grouper and IBCs as well as necrotic cells were determined to be the pathognomonic sign of megalocytivirus-infected coral trout grouper.

Substitution of fishmeal with soybean meal in humpback Grouper, Cromileptes altivelis juvenile diets supplemented with phytase Rachman Syah; Usman Usman; Makmur Makmur; Taufik Ahmad
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Feeding experiments were conducted to evaluate the effects of replacing fishmeal with soybean meal in diet on growth of humpback grouper. Fifteen cages of 1 x 1 x 1.2 m3 each stocked with 16 humpback grouper juveniles (61.3 ± 0.4 g/pc) were set up randomly in seawater. Fish were fed to satiation twice daily for 112 days. The control diet contained 61.9% fishmeal (63.34% crude protein). Four isonitrogenous (48% crude protein) and isocaloric (4.7 kcal/g feed) diets supplemented with commercial phytase “Rhonozyme-P” at 0.075% were formulated to contain different levels (8%, 16%, 24%, and 32%) soybean meal (43.65% crude protein) as a partial replacement for fishmeal. These diets contained total phosphorus levels between 3.6—4.5 (±0.4) % and 0.7—1.5 (±0.04) % available phosphorus. Replacement of fishmeal with soybean meal (8 to 32% replacement) was not significantly different (P>0.05) to the control diet on daily growth rate (DGR), food conversion ratio (FCR), protein efficiency ratio (PER), and daily food consumption (DFC). However, the dietary levels of soybean meal significantly affected (P<0.05) whole body protein and phosphorus retention (Table 1). These data suggest that addition of phytase in diets could improve protein and phosphorus availability and reduce nitrogen and phosphorus loading in the environment. Phytase can therefore play an important role in formulating eco-friendly feed for humpback grouper. Based on P loading, supplementation of phytase enable up to 24% fishmeal replacement with soybean meal.

THE EFFECT OF VITAMIN C (L-ASCORBYL MONOPHOSPHATE-Mg) ON THE DEFORMITY PERFORMANCE OF HUMPBACK GROUPER (Cromileptes altivelis) LARVAE Sophia Lasma Sagala; Suko Ismi; I Nyoman Adiasmara Giri
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

This study was aimed to get a standard protocol for vitamin C application in enrichment of Artemia nauplii and to examine the impact of vitamin C on humpback grouper larval deformity performance. Vitamin C in the form of L-ascorbyl-2-monophosphate-Mg (AMP-Mg) was used for the enrichment and incorporated into Artemia nauplii at a given level of 0-1.5 g/L with enrichment period of 4, 6, and 24 hours for each level. The effect of AMP on larval deformity was tested using the following treatments: unenriched both Artemia nauplii and commercial diet, un-enriched Artemia nauplii and experimental diet containing 2.5 g AMP-Mg/kg diet, enriched Artemia nauplii obtained from the protocol and commercial larval diet. Analysis of vitamin C content in Artemia nauplii was carried out using HPLC and larval deformities were observed using staining method, visual examination, or X-ray depending on larval age. The results showed that the best enrichment method of AMP in Artemia nauplii is achieved at the dose of 0.9 g/L for 6 h. The lowest percentage of abnormalities was found in larvae fed with enriched Artemia nauplii and commercial diets, suggesting that vitamin C has an important role in controlling larval deformities.

PHYLOGENETIC TREE ON LERNAEOSIS IN ARWANA FISH (Scleropages jardinii) Dikry Novel Shatrie; Kurniasih Kurniasih; Wisnu Nurcahyo; Triyanto Triyanto
Indonesian Aquaculture Journal Vol 6, No 1 (2011): (June 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Arwana Irian fish is one of the endangered species. Some studies on arwana Irian fish found that Lernaeosis attacked arwana Irian fish. Lernaeosis is one of the diseases that cause the high mortality in juvenile fish. The objectives of this research was to find out the species of Lernaea (Copepoda) often attacked arwana Irian fish. Lernaea sp. was collected from Papua and Jakarta (Java). They were fixed in the ethanol absolute solution for DNA sequencing in 28S DNA region with primer 28SF (5’–ACA ACT GTG ATG CCC TTA G–3’); 28SR (5’ TGG TCC GTG TTT CAA GAC G–3’). It was found five different species of Lernaea and one of them was thought as a new species, based on the morphology. However, based on the phylogenetic analysis, they showed three different groups. Lernaea cyprinacea G., L. papuensis, L. devastatrix, and L. lophiara were in one group; L. cyprinacaea and L. oryzophila were in one groups; and the new Lernaea sp. was in the different group.

INDUCED FUCTIONAL MALE OF CORAL TROUT GROUPER (Plectropomus leopardus) USING 17α-METHYLTESTOSTERONE HORMONE Sar Budi Moria Sembiring; Jhon Harianto Hutapea; Ida Komang Wardana; Ahmad Muzaki; Indah Mastuti
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The success of grouper seeds production is depends on the availability of qualified broodstock. The nature of grouper is protogynous hermaphrodite, causing difficulties to mantain female and a bit difficult to get male broodstock, one possibility to accelerate sex revers is by hormone manipulation. The aim of this experiment was to find effectiveness of 17α-methyl testosterone hormone to produce coral trout grouper (Plectropomus leopardus) functional male. The experiment was conducted in floating net cage by using 6 net cages with size of 2 m x 2 m x 2 m at density of 25 fish/cage, size of fish were 377.27±21.49 g. The fishes were treated by hormone implantation at concentration of 50 μg/kg body weight and without hormone implantation as a control with 3 replicates. The results showed that the highest concentration of testosteron in fish blood (1.144±0.135 pg/mL) was detected after four months of hormone treatment, but the concentration of testosteron in fish blood declined after 8th months of treatment. The treated fish with hormone grew faster than control. Based on histological analysis of gonad, female gonado somatic index was higher for treated fish compare to control. Its seems that hormone tratment lead to promote development of female maturity and than sex reverse into male become faster.

Streptococcus agalactiae INFECTION ON TILAPIA (Oreochromis niloticus) IN CIRATA RESERVOIR, WEST JAVA Angela Mariana Lusiastuti; Lila Gardenia; Tatik Mufidah; Yani Aryati
Indonesian Aquaculture Journal Vol 4, No 1 (2009): (June 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Streptococcosis is one of bacterial diseases in the culture of Tilapia, Oreochromis niloticus and has caused significant economic losses. Streptococcus iniae, is known as pathogen to marine and freshwater fishes whereas Streptococcus agalactiae is known as pathogen to Tilapia. The isolation and characterization of four isolates of S. agalactiae, were described from an infected Tilapia from Cirata Reservoir, West Java, in July 2008. Conventional and rapid identification systems were used to determine isolates of S. agalactiae from brain and kidney tissues. In this paper, we have characterized S. agalactiae and this was the first isolation of this bacteria from fish. The isolates were gram positive, catalase-negative, oxidase-negative,  haemolytic cocci colonies on blood agar. All of the of isolates were biochemically characterized with the API 20 Strep System (bioMerieux). Bacterial chromosomal DNA used in PCR assay was extracted by heating method. The forward primer is Sdi 61: 5’-AGGAAACCTGCCATTTGCG-3’ and the reverse primer is Sdi 252: 5’-CAATCTATTTCTAGATCGTGG-3’ with gene target 16S intergenic spacer and it has 192 bp in length. These primers were designed by Alpha DNA (Montreal, Quebec). The biochemical patterns of four isolates were rather different although almost all traits were similar with the exception of pyroglutamic acid (pyra) and L-arginin (ADH), for which we observed negative and positive reaction in this study. Therefore, some of the biochemical characteristics of the four isolates did not fit 100% with the typical patterns of S. agalactiae. However, the PCR result showed that this PCR assay is an effective tool for rapid and specific detection of S. agalactiae, the main pathogens involved in warm-water streptococcosis, obtained from pure culture of naturally infected fish. Therefore, it could be a useful alternative for culture-based routine diagnosis of warm-water streptococcal infections in fish.

EFFECTS OF CURRENT VELOCITY ON FILTRATION AND INGESTION RATE OF PEARL OYSTER (Pinctada maxima) Apri I. Supii; Dewi Puji Lestari; Sudewi Sudewi
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Pinctada maxima is a filter feeder organism obtaining food particles by filtration from water suspension. Filtration and ingestion rate of a large number of benthic filter feeders in a variety of coastal environment have been known influenced by current velocity. The purposes of this study were to examine the effects of current velocity on filtration and ingestion rate of pearl oyster P. maxima and to determine the best current velocity. Randomized Block Design was used in this experiment with four current treatments of 8, 16, 24, and 32 cm s-1. The results of this experiment demonstrated that current velocity has a marked effect on filtration rate of P. maxima and that this species follows the general pattern of response observed for other bivalves. Different velocity affected filtration and ingestion rate in pearl oyster P. maxima. It was gradual decline in the suspension feeding with increasing velocity. Filtration rate of bivalves with siphons facing into the current, were directly inhibited by hydrodynamic effects on the functioning of the bivalves pump. At increasing current velocity, pressures applied to the inhalant and exhalant aperture facing into the flow would result in higher pressures than that of produced by the ciliary pump, resulting in reduced filtration rate. The highest filtration and ingestion rate in this experiment were obtained at velocity of 8 cm s-1 that achieved 3.409±0.485 l/h and 37.91±1.27 (x107) cells/h, respectively.

THE EFFECT OF MARINE SPONGE Aaptos aaptos EXTRACT IN VIBRIOSIS TREATMENT OF BLACK TIGER SHRIMP Penaeus monodon LARVAE Rosmiati Rosmiati; Najiah Musa; Habsah Mohammad; Tengku Sifzizul Tengku Muhammad; Andi Parenrengi; Wahyuni Wahyuni
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Black tiger shrimp Penaeus monodon post larvae were challenged with Vibrio harveyi and butanol extract of selected marine sponge Aaptos aaptos to determine its antibacterial bioactive potential in vibriosis treatment. Based on the preliminary toxicity study, the A. aaptos butanol extract with concentrations of 31.25, 62.5, and 125 mg/L were selected in the study. Black tiger shrimp post larvae were challenged with V. harveyi at 107 cfu/mL and immersed A. aaptos butanol extract with the concentration of 125 mg/L showed significantly in (P<0.05) decrease mortality of the post larvae treated. Besides at this concentration, V. harveyi population in the rearing water and the post larvae treated decreased compared to control (untreated post larvae). Histological observation indicated that there was no changing on hepatopancreas of the black tiger shrimp post larvae. Based on this result, it is suggested that the butanol extract of A. aaptos is a potential bioactive compounds source in the treatment of vibriosis which may replaced the current antibiotics application.

Page 21 of 34 | Total Record : 334

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Ofan Bosman

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