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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 334 Documents

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Lagenidium callinectes INFECTION ON ROTIFERS Brachionus sp. Des Roza; Fris Johnny
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Milkfish, Chanos chanos and humpback grouper, Cromileptes altivelis hatcheries have developed at Gondol, Bali since 1995 and until now still rely on rotifers, the main natural food, supply. Recent problem on mass culture of rotifer, Brachionus sp. is harvest failure caused by fungus infection. Under light microscope, infected eggs and bodies of the rotifers was filled with numerous aseptate hyphae. Two isolates of fungi were isolated from rotifer eggs and carcass on June 21st, 2004 and on June 25th, 2004 obtained from milkfish and humpback grouper hatcheries at Gondol. Based on its morphological characteristics, the pathogenic fungus was identified as Lagenidium callinectes which grows optimally at 25°C and survives in 1.0%, 2.5%, and 5.0% NaCl as well as in 1.0% and 2.5% KCl. Both of the present isolates utilize only 8 out of 26 carbohydrates and derivatives tested as carbon, nutrition and energy sources. This finding is the first report on rotifer, Brachionus sp. infected with L. callinectes causing up to 100% mortality.

IN VITRO ANTAGONISTIC ACTIVITIES OF INDONESIAN MARINE SPONGE AAPTOS AAPTOS AND CALLYSPONGIA PSEUDORETICULATA EXTRACTS AND THEIR TOXICITY AGAINST Vibrio spp. Rosmiati Rosmiati; Habsah Mohamad; Tengku Sifzizul Tengku Muhammad; Najiah Musa; Aziz Ahmad; Noraznawati Ismail; Farida Mohamad; Nurhidayah Nurhidayah
Indonesian Aquaculture Journal Vol 6, No 2 (2011): (December 2011)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Vibriosis is one of diseases which often results in mass mortality of Penaeus monodon larval rearing systems. It attacks shrimp of all stages in zoea, mysis and shrimp postlarva stage. This disease is caused by Vibrio spp, particularly Vibrio harveyi (a luminescent bacterium). Several kinds of antibiotics and chemical material have been used to overcome the disease but they have side effects to environment and human. The searching of bioactive compounds as an alternative treatment has been done for multi purposes. In this study diethyl eter, butanol and aqueous extract of Indonesian sponges Aaptos aaptos and Callyspongia pseudoreticulata were tested for in vitro activity against Vibrio spp. and Vibrio harveyi by using disc diffusion method. The result showed that all extracts of Aaptos aaptos gave a positive antibacterial activity towards those pathogenic bacteria. Meanwhile, only butanol extract of Callyspongia pseudoreticulata obtained to exhibit an antibacterial activity on those pathogenic bacteria. The strong anti-vibrio activity were shown by butanol and aqueous extract of Aaptos aaptos with the minimum inhibitory concentration (MIC) value of 0.313 and 0.625 mg/mL, respectively. Whilst, the butanol extract of Callyspongia pseudoreticulata indicated a low antibacterial activity with the MIC value of 10 mg/mL. Toxicity of those active extracts was evaluated by Brine Shrimp Lethality Test (BST). Interestingly, butanol and aqueous extracts of Aaptos aaptos did not show any toxic effect in Artemia salina larvae up to 8 x MIC (2.504 mg/mL and 5.000 mg/mL). It is the first report for the anti-vibr io activity of both Aaptos aaptos and Callyspongia pseudoreticulata. This results suggest that Aaptos aaptos has a potential to be used as a source of alternative compound to vibriosis prevention for mariculture.

FATTY ACID COMPOSITION AND PROSTAGLANDIN CONTENT OF THE RED SEAWEED Gracilaria sp. FROM INDONESIA Muhammad Ikbal Illijas; Arifuddin Arifuddin; Luqman Saleh; Yutaka Itabashi
Indonesian Aquaculture Journal Vol 7, No 1 (2012): (June 2012)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

High content of polyunsaturated fatty acids (PUFAs) such as arachidonic and eicosapentaenoic acids are typical for the red alga. Analysis of fatty acid composition and prostaglandin content was conducted in the red alga Gracilaria sp. from Indonesia. Total lipid of the alga was extracted with CHCl3-MeOH (2:1, v/v). Analysis of the fatty acids composition was performed on gas chromatography (GC) equipped with omega wax column (30 m x 0,32 mm i.d., Supelco, PA, USA) and analysis of prostaglandins were carried out by HPLC on ODS column (Mightysil RP-18 GP, 250 mm x 4.6 mm, 5 μm). The content of fatty acids high for were palmitic acid (50%) and arachidonic acid (26.9%), whereas prostaglandin E2 was identified and found lower concentration (44.2 μg/gram total lipid).

GROWTH RESPONSE OF CLOWN LOACH (Chromobotia macracanthus Bleeker 1852) JUVENILES IMMERSED IN WATER CONTAINING RECOMBINANT GROWTH HORMONE Asep Permana; Alimuddin Alimuddin; Wartono Hadie; Agus Priyadi
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The main problem in the culture of clown loach (Chromobotia macracanthus) is the slow growth rate, which takes about six months to reach its market size (two inches total body length). Slow growth eventually cause a long production time and increase the production costs. An alternative solution can be proposed in order to enhance the growth is by using recombinant growth hormone. The aim of this study was to determine the immersion dose of recombinant Epinephelus lanceolatus growth hormone (rElGH) which can generate the highest growth in clown loach. Larvae at seven day after hatching were hyperosmotic treated with NaCl 2.0% for one minute, then immersed for one hour in water containing 0.3% NaCl, 0.01% bovine serum albumin (BSA), and different doses of rElGH, namely: 0.12 (treatment A), 1.2 (B), 12 (C), and 120 mg/L (D). As control, fish were immersed in water without rElGH and NaCl (control-1), water containing 0.3% NaCl and 0.01% BSA (control-2), and 0.3% NaCl water (control-3). Each treatment was replicated three times. The results showed that clown loach juveniles in treatment B, C, and D had longer total body length (P<0.05) than control-1, while fish treatment A was the same as controls. Survival and body weight were similar in all treatments and controls (P>0.05). In addition, the percentage of large size juveniles increased approximately 5% in treatment B, almost the same as in the medium size, while the small size were decrease compared to the control-1. Thus, the best immersion dose of rElGH was 1.2 mg/L water.

OVER-EXPRESSION OF GENE ENCODING FATTY ACID METABOLIC ENZYMES IN FISH Alimuddin Alimuddin; Goro Yoshizaki; Toshio Takeuchi; Odang Carman
Indonesian Aquaculture Journal Vol 3, No 2 (2008): (December 2008)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3) have important nutritional benefits in humans. EPA and DHA are mainly derived from fish, but the decline in the stocks of major marine capture fishes could result in these fatty acids being consumed less. Farmed fish could serve as promising sources of EPA and DHA, but they need these fatty acids in their diets. Generation of fish strains that are capable of synthesizing enough amounts of EPA/DHA from the conversion of α-linolenic acid (LNA, 18:3n-3) rich oils can supply a new EPA/DHA source. This may be achieved by over-expression of genes encoding enzymes involved in HUFA biosynthesis. In aquaculture, the successful of this technique would open the possibility to reduce the enrichment of live food with fish oils for marine fish larvae, and to completely substitute fish oils with plant oils without reducing the quality of flesh in terms of EPA and DHA contents. Here, three genes, i.e. Δ6-desaturase-like (OmΔ6FAD), Δ5-desaturase-like (OmΔ5FAD) and elongase-like (MELO) encoding EPA/DHA metabolic enzymes derived from masu salmon (Oncorhynchus masou) were individually transferred into zebrafish (Danio rerio) as a model to increase its ability for synthesizing EPA and DHA. Fatty acid analysis showed that EPA content in whole body of the second transgenic fish generation over-expressing OmΔ6FAD gene was 1.4 fold and that of DHA was 2.1 fold higher (P<0.05) than those in non-transgenic fish. The EPA content in whole body of transgenic fish over-expressing OmΔ5FAD gene was 1.21-fold, and that of DHA was 1.24-fold higher (P<0.05) than those in nontransgenic fish. The same patterns were obtained in transgenic fish over-expressing MELO gene. EPA content was increased by 1.30-fold and DHA content by 1.33-fold higher (P<0.05) than those in non-transgenic fish. The results of studies demonstrated that fatty acid content of fish can be enhanced by over-expressing gene encoding enzymes involved in fatty acid biosynthesis, and perhaps this could be applied to tailor farmed fish as even better sources of valuable human food.

THE OPTIMAL RATIO OF NILE TILAPIA (Oreochromis niloticus) AND COMMON CARP (Cyprinus carpio) FOR IMPROVING PRODUCTIVITY ON DEEP WATER POND Imam Taufik; Sutrisno Sutrisno; Eri Setiadi
Indonesian Aquaculture Journal Vol 8, No 1 (2013): (June 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Pond productivity can be increased by applied polyculture system in the deep pond. The purpose of this experiment is to examine the optimal ratio between nile tilapia and common carp, in order to increase the productivity. Nine concrete tanks (15 m2) with water depth of 2.2 m and were completed by water inlet, water outlet, and aeration. Both of nile tilapia and common carp with size ranging of 5-8 cm in total length were used. Stock density was 150 ind./m2. The difference ratio of both fish tilapia and carp of fish stocked as a treatment. The fish ratio this experiment were as followed: A) 100%; B) 80%:20%; C) 60%:40%. Fish fed by pellet until at ad libitum. The duration of experiment was 100 days. Parameters such as survival, growth, and productivity were observed every ten days during the experiment period. Water quality parameters were also periodically observed. The results showed that survival of nile tilapia among the treatments were not significantly different (P>0.05) where survival of common carp at B treatment was better than C treatment (P<0.05). The highest of growth of absolute weight (94.86±2.85 g) and total length (14.71±1 cm) of nile tilapia at B treatment was found (P<0.05) where the best of growth of absolute weight (106.52±10.47 g) and total length (11.57±1.78 cm) of common carp was also found at B treatment (P<0.05). Biomass productivity at B treatment was the highest compared with A treatment (P<0.05). Combination between polyculture and the deep water pond technology could increase productivity. The polyculture system and the deep water pond technology would be able to keep constant water quality within in the threshold accordance with the regulation for fish culture.

MORPHOLOGICAL CHARACTERISTIC OF SPERMATOGONIA AND TESTES DISSOCIATION : A Preliminary Study for the Germ Cell Transplantation in Giant Gouramy (Osphronemus gouramy) Irma Andriani; Ita Djuwita; Komar Sumantadinata; Muhammad Zairin Junior; Harton Arfah; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 5, No 2 (2010): (December 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The recent study were attempting to develop spermatogonial germ cell transplantation as a tool to preserve and propagate male germ-plasm from endangered fish species, as well as to produce surrogate broodstock of commercially valuable fish. Spermatogonia identification and testes dissociation were the first necessary steps to obtain highly amount and viable population of spermatogonia as donor cells for transplantation. Using giant gouramy testes as a model, spermatogonia was histological characterized and two methods of testes dissociations were compared (i.e. medium A contained 0.5% trypsin in PBS and medium B contained 0.5% trypsin and DNase 10 IU/μL in PBS complemented with CaCl2, Hepes and FCS). Optimal incubation times (1, 2, 3, 4 and 5 hours) in dissociation medium were also determined. Freshly isolated testes of immature giant gouramy were minced in dissociation medium and then incubated to get monodisperce cell suspension. Parameters observed were number and viability of spermatogonia (ø > 10 μm). The viability was analyzed using trypan blue exclusion dye. The results showed that the average number of spermatogonia observed in medium B was higher than in medium A (P<0.05), meanwhile the viability of spermatogonia between medium A and B were not significantly different (P>0.05). The viability of spermatogonia decreased by the increasing duration time of dissociation. The viability of spermatogonia started to decrease significantly in 2 hours incubation time in medium A and 4 hours incubation time in medium B (P<0.05). In conclusion, application of dissociation medium B yielded higher number of viable spermatogonia than dissociation medium A.

THE EFFECTS OF WEANING TIME ON THE GROWTH AND SURVIVAL OF MUD CRAB (Scylla olivacea)THE EFFECTS OF WEANING TIME ON THE GROWTH AND SURVIVAL OF MUD CRAB (Scylla olivacea) Haryati Haryati; Yushinta Fujaya; Early Septiningsih
Indonesian Aquaculture Journal Vol 13, No 2 (2018): (December, 2018)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Live foods such as rotifers and Artemia are commonly used as foods in larval rearing of mud crab (S. olivacea). However, the continuous availability and nutritional consistency of live foods are difficult to control. Thus, the development of artificial diets to partially or fully replaced live foods is needed to overcome the limitations of live foods. The purpose of this research was to determine the best stage at which mud crab larvae can be weaned from live foods to artificial diets. The research experiment consisted of: treatment-1, the larvae were fed with live foods from zoea-1 to megalopa stages as the control treatment; treatment-2, the larvae were fed with artificial diet from zoea-2 to megalopa stages; and treatment-3, the larvae were fed with artificial diet from zoea-3 to megalopa stages. In treatment-4, artificial diet was given from zoea-4 to megalopa stages. The growth and survival rate of larvae in treatment-1, 3, and 4 were not significantly different (P>0.05) but significantly different with treatment-2. Based on the present results, this study suggests that artificial diet can be given to mud crab larvae (S. olivacea) from the third zoea stage.

CORRELATION OF MICROSATELLITE DNA MARKERS WITH GROWTH TRAITS IN STRIPED CATFISH (Pangasianodon hypophthalmus) Huria Marnis; Evi Tahapari; Jadmiko Darmawan
Indonesian Aquaculture Journal Vol 13, No 2 (2018): (December, 2018)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Marker-assisted selection in genetic improvement of striped catfish is useful in the breeding program of the fish. Five microsatellite markers were characterized in the largest (4.03 kg ± 1.67 kg) and smallest (1.41 kg ± 0.22 kg) individuals. Five polymorphic loci were then used to genotype 160 individuals and the associations between their genotypes and growth traits were examined. The result showed that twenty-seven alleles were detected in striped catfish. The number of alleles per locus (NA) ranged from 4 to 7, with an average of 5.4 alleles per locus. The effective number of alleles per locus (NE) ranged from 3.940 to 6.939, with an average of 5.32 alleles per locus. HO and HE ranged from 0.125 to 0.944 (mean value of 0.472) and from 0.564 to 0.775 (mean value of 0.697), respectively. PIC ranged from 0.573 to 0.799 (mean value of 0.706), showing that they were highly polymorphic loci. Only one microsatellites loci (Pg13) that showed significant differences (P<0.01) in the associations between their genotypes and growth traits, while Pg3 and Pg14 were significantly associated with the standard length (P<0.01) and body weight (P<0.05). However, the Pg1 and Pg2 were not significantly associated with the body weight and standard length. Four genotypes of three loci were positively correlated with the growth traits (body weight and standard length) i.e. genotypes 194/194 for Pg3, 227/227 and 229/229 for Pg13, 279/279 for Pg14. These four genotypes can be used to identify growth traits in the molecular marker-based selection of a breeding program.

ISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF PROTEOLYTIC BACTERIA TO IMPROVE PROTEIN DIGESTIBILITY OF FISH FEED INGREDIENTSISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF PROTEOLYTIC BACTERIA TO IMPROVE PROTEIN DIGESTIBILITY OF FISH FEED INGREDIENTS Muyasari Mulyasari; Mas Tri Djoko Sunarno; Lusi Herawati Suryaningrum
Indonesian Aquaculture Journal Vol 13, No 2 (2018): (December, 2018)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Protease is one of the potential enzymes used to improve the quality of local raw materials as fish feedstuff. The aim of this research was to find high protease activity-bacteria isolates in improving protein digestibility of fish feedstuff. Isolation was conducted on various growth substrates such as water and sediment of hot springs, mangrove sediment, and shrimp head waste. The parameters used in the selection and characterization of potential proteolytic bacteria were qualitative and quantitative tests of proteolytic activity. Microbes that have the highest enzyme activity were then selected and characterized based on their growth curve, production time of protease, and determination of optimum condition (temperature and pH) of protease activity. The result showed that there are only 18 isolates from 44 isolates positively had protease activity (qualitatively). Three isolates with the highest activity (RGL1.1, PL1.1, and PL1.2) qualitatively and quantitatively were then selected to be tested for their pathogenecity on tilapia. The results showed that only RGL1.1 was not pathogenic and therefore, potentially used as a protease producer candidate. The characterization of RGL1.1 isolates showed that the exponential phase of RGL1.1 was thirty-sixth hour in line with the optimum activity of enzyme production. The optimum pH and temperature of RGL1.1 isolate were 6 and 50°C, respectively. RGL1.1 isolate is a Gram-positive rod-shaped bacteria that has 99.53% of resemblance to Bacillus cereus.

Page 22 of 34 | Total Record : 334

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Contact Name
Ofan Bosman

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Ofan Bosman

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Journal Mail Official
iaj.puslitbangkan@gmail.com

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Kota adm. jakarta pusat,

Dki jakarta

INDONESIA