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Indonesian Aquaculture Journal

iaj
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Published by Kementerian Kelautan dan Perikanan RI

ISSN : 02150883 EISSN : 25026577 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Indonesian Aquaculture Journal is a peer-reviewed and open access journal based in Indonesia that globally/internationally accepts and publishes scientific articles in the field of aquaculture. The journal is hosted and managed by the Center for Fisheries Research, Indonesian Ministry of Marine Affairs and Fisheries and serving as a scientific platform to share research information in and contribute to the development of various disciplines of aquaculture including genetics, reproduction, nutrition and feed, fish health and diseases, engineering, and environmental assessment.

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Articles 334 Documents

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GENETIC IDENTIFICATION FOR TUNA AND RAINBOW RUNNER CAPTURE IN NORTH BALI WATERS Gusti Ngurah Permana; Jhon Harianto Hutapea; Haryanti Haryanti; Sari Budi Moria Sembiring; Akio Nakazawa
Indonesian Aquaculture Journal Vol 1, No 1 (2006): (June 2006)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Gondol Research Institute for Mariculture identification of tuna and rainbow runner was an objective in this current study. Samples of five species were collected from territorial water of North Bali. The method used in this study was allozyme electrophoresis. The results showed that buffer of CAPM-6 (citric acid aminoprophylmorpholine) resulted in a sharp and clear banding pattern. The species could be differentiated in six diagnostic isozyme patterns Idh* (isocitrate dehydrogenase), 6Pgd* (6 phosphogluconate dehydrogenase), Gpi* (glucose phosphate isomerase), Mdh* (malate dehydrogenase), Est* (esterase), and Sp* (sarcoplasmic protein). All species were in Hardy-weinberg equilibrium. Heterozygosities of species were ranged from 0.00 to 0.099. Yellowfin tuna has the highest heterozigosity compared with the other species. Clustering samples according to pairs revealed that genetic distance of Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) had small value (0.001). By contrast, the largest value was observed between yellowfin tuna, T. albacares and rainbow runner, E. bipunnulata (0.007). This value indicated that Bullet tuna (A. rochei) and Eastern little tuna (E. affinis) closed relation, while among yellowfin tuna, skipjack tuna, and rainbow runner, were separated phylogenically.

EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio koi Sekar Ayu Chairunnisa; Sri Nuryati; Alimuddin Alimuddin; Sri Murtini; Ayi Santika; Dwi Hany Yanti
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Koi herpesvirus (KHV) is one of the major pathogen for koi and common carp which cause high mortality and economic losses for the farmer. The purpose of this study was to determine the efficacy of glycoprotein-11 (GP-11) KHV DNA vaccine and compared to GP-25 KHV DNA vaccine. The vaccine in the form of naked DNA plasmidwas delivered by intramuscularly injection to the 3-month-old koi. The fish were divided into six groups, i.e. unvaccinated group (negative control C- and positive control C+), and vaccinated group (2.5 μg/100 μl of GP-11 (group 1), 7.5 μg/100 μl of GP-11 (group 2), 12.5 μg/100 μl of GP-11 (group 3), and 12.5 μg/100 μl of GP-25 (group 4)). At day 42 post vaccination, all fish of each groups were challenged by injecting KHV titre 10-3 FID50. Number of dead fish was counted everyday after the challenge until 30 days. The results showed that vaccinated fish were had survival rate of 83.33-93.33% (group 2, 3 and 4). It’s show that GP-11 KHV DNA vaccine has high efficacy. As conclusion, GP-11 DNA vaccine could be an alternative DNA vaccine for preventing KHV infection.

SYSTEMATIC REVISION OF THE GENERA OF PANGASIIDAE (SILURIFORMES, OSTARIOPHYSI) Rudhy Gustiano; Laurent Pouyaud
Indonesian Aquaculture Journal Vol 3, No 1 (2008): (June 2008)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The family Pangasiidae belongs to the suborder Siluroidei, order Siluriformes, and suborder Ostariophysi. Since the groups were established as Pangasiini Bleeker, 1858; its content and classification have been greatly changed. Judging from the literature, the main constraint to cultivate wild species and to optimize the production of cultured species is needed to the poorly documented systematics of this family. Therefore the objective of the present study is to provide the diagnostic characters and the keys for identification the genera of Pangasiidae. The results clearly demonstrate that biometrically four natural groups can be distinguished. They are the genus Helicophagus, Pangasianodon, Pteropangasius, and Pangasius. The diagnosis of the family, the identification key of the genera and the description are given.

MORPHOLOGICAL DEVELOPMENT OF Napoleon WRASSE, Cheilinus undulatus LARVAE Jhon Harianto Hutapea; Bejo Slamet
Indonesian Aquaculture Journal Vol 1, No 2 (2006): (December 2006)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

To improve the survival rate, napoleon wrasse larval rearing trial was conducted at Research Institute for Mariculture, Gondol-Bali in 2003. The trial aims at assessing initial feed for larvae, food habit, and morphological development from early larval stage to juvenile. The results showed that chicken egg yolk could be applied as initial feed and followed by rotifer, Artemia and mysid (Mesophodopsis sp.). Three swimming behavior of larvae were observed, drifting, free swimming and hiding on the substrate as larvae develop. Digestive system development, simple tube like, transition stage and coiled where digestive system could be distinguished between stomach, intestine and rectum.

INFLUENCE OF FEEDING TIME ON FEED UTILIZATION BY SIAMESE CATFISH, Pangasius hypophthalmus JUVENILE Nina Meilisza; Yann Moreau; Ettiene Baras; Rina Hirnawati
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Siamese catfish (Pangasius hypophthalmus) at juvenile stage is used as ornamental fish by fish hobbies because of their beautiful shape and movement. Feeding time is a part of feeding management to get the best and effective strategy on feed input. This study aimed to determine the growth of Siamese catfish juvenile by looking at the influence of feeding time on feed utilization. The average individual weight of juveniles used was ± 3.2 g stocked 15 fish per aquarium equipped with recirculation system. Two level factors in the factorial design consisted of 4 feeding times (at 8:00, at 12:00, at 16:00, and as control (8:00, 12:00, 16:00) and feeding level (45 g feed kg-1 fish, 90 g feed kg-1 fish). Interaction between feeding time and feeding level resulting eight types of treatments research, namely A (8/45), B (12/45), C (16/45), D (8:12:16/45), E (8:12:16/90), F (8/90), G (12/90), and H (16/90). The results showed that feeding time had significant effect on the specific growth rate, feed conversion, and protein efficiency ratio and were significantly different among the treatments (P<0.05). Besides feeding time control (treatments D and E), treatment C also showed the highest of specific growth rate (2.03%), protein efficiency ratio (1.46), and the lowest feed conversion ratio (1.85).

CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798) Andi Parenrengi; Alimuddin Alimuddin; Andi Tenriulo
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

White spot syndrome virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon) culture and adversely affects prawn industry worldwide including Indonesia. It is well known that the protein structure of WSSV plays an important role in the virus infection and morphogenesis process. A viral protein structure called VP-15 is located in the nucleocapsid of virion virus. The protein structure involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could be involved in constructing the RNA interference (RNAi), so it is needed to isolate and characterize for RNAi technology purpose. The study was aimed to isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The characterization of VP-15 was undertaken through assessment of nucleotide sequence, amino acid deduction, alignment nucleotide/protein searches using Genetyx and BLAST program, and dendrogram construction analysis. The results showed that VP-15 was successfully isolated in form of ORFDNA with a fragment size of 243 bp. The phylogenetic tree analysis revealed three clusters corresponding to the time (year) of isolates collection. The VP-15 consisted of 80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%), followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine (24.6%). The successful isolation of VP-15 is a very important step in providing a basic yet suitable material in constructing the dsRNA vaccine to control shrimp diseases in aquaculture.

MICROBIAL COMMUNITY OF BLACK BAND DISEASE ON INFECTION, HEALTHY, AND DEAD PART OF SCLERACTINIAN Montipora sp. COLONY AT SERIBU ISLANDS, INDONESIA Ofri Johan; Dietriech Geoffrey Bengen; Neviaty Putri Zamani; Suharsono Suharsono; David Smith; Angela Mariana Lusiastuti; Michael Sweet
Indonesian Aquaculture Journal Vol 9, No 2 (2014): (December 2014)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

It is crucial to understand the microbial community associated with the host when attempting to discern the pathogen responsible for disease outbreaks in scleractinian corals. This study determines changes in the bacterial community associated with Montipora sp. in response to black band disease in Indonesian waters. Healthy, diseased, and dead Montipora sp. (n = 3 for each sample type per location) were collected from three different locations (Pari Island, Pramuka Island, and Peteloran Island). DGGE (Denaturing Gradient Gel Electrophoresis) was carried out to identify the bacterial community associated with each sample type and histological analysis was conducted to identify pathogens associated with specific tissues. Various Desulfovibrio species were found as novelty to be associated with infection samples, including Desulfovibrio desulfuricans, Desulfovibrio magneticus, and Desulfovibrio gigas, Bacillus benzoevorans, Bacillus farraginis in genus which previously associated with pathogenicity in corals. Various bacterial species associated with uninfected corals were lost in diseased and dead samples. Unlike healthy samples, coral tissues such as the epidermis, endodermis, zooxanthellae were not present on dead samples under histological observation. Liberated zooxanthellae and cyanobacteria were found in black band diseased Montipora sp. samples.

ONTOGENY OF THE BALASHARK Balantiocheilos melanopterus BLEEKER, 1851 (CYPRINIDAE) Etienne Baras; Agus Priyadi; Marc Legendre
Indonesian Aquaculture Journal Vol 2, No 1 (2007): (June 2007)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The Balashark is a highly praised ornamental species that has been overfished to the point that it has become endangered in most of its distribution area, especially in Indonesian waters. Captive propagation has reduced the threats upon wild populations of balashark. Nevertheless, knowledge on the biology of this species is still scarce, in particular as regards to its ontogeny. This information is crucial for the identification of larvae and small juveniles in the wild, as well as for improving its culture. Balashark eggs average 1.2-1.3 mm in diameter and hatch after 13 hours (26-28°C), giving birth to 4.5-mm embryos, with a yolk sac of circa 1.1 mm3. At the end of the endogenous feeding period (day 4 after hatching [AH]), larvae attain 6.3 mm TL. The mouth opens on day 2 AH (5.8 mm total length, TL). At the time of first feeding (day 4 or 5 AH, 6.3 mm TL), their mouth and gape already average 0.5 mm and 0.27 mm, respectively, and they grow up to 10.2 and 6.3% TL, respectively, on day 8 AH. The swim bladder starts forming as early as day 2 AH and is filled by day 3 AH (5.9 mm TL), but the separation between the anterior and posterior chambers does not take place before 9.5 mm TL (day 9 or 10 AH). The fin development sequence is typical of cyprinids, and follows a caudal-to-cranial pattern (i.e.; caudal, dorsal and anal, pelvic then pectorals), except for the anlagen of pectoral fins, which are present in 1-day old fish (5.3 mm TL). Based on the vanishing of the abdominal and caudal fin fold, and on the lengths of the fins relative to fish size, the transition between the larval and juvenile stage occurs at circa 17 mm TL (corresponding standard length: 13.7 mm). At this stage, the scale cover is not developed yet, but juveniles already exhibit the typical pigmentation pattern of adults on their dorsal, anal, pelvic and caudal fins, while pigmentation on the pelvic fins is still in its very early stages.

EFFECTIVITY OF INACTIVE GSDIV (GROUPER SLEEPY DISEASE IRIDOVIRUS) VACCINE IN GROUPER FISH (Cromileptes altivelis and Epinephelus fuscoguttatus) AGAINST GSDIV INFECTION Ketut Mahardika; Indah Mastuti; Haryanti Haryanti
Indonesian Aquaculture Journal Vol 8, No 2 (2013): (December 2013)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Grouper sleepy disease iridovirus (GSDIV) has been known as viral agent causing mass mortality and significant economic losses in Indonesian aquaculture industry. The aim of this research was to know the effectivity of formalin-inactivated GSDIV vaccine to prevent GSDIV infection in humpback grouper (Cromileptes altivelis) and tiger grouper (Epinephelus fuscoguttatus). The vaccine was derived from GF cells infected-GSDIV which activated using formalin. Used vaccine was contained titer virus of 102,80-105,80 TCID50/ mL. Result of vaccination test indicated that a vaccinated grouper fish with formalininactive GSDIV vaccine should decreased mortality rate of 5%–85.4% in humpback grouper and tiger grouper that infected with GSDIV. Histopathologically, infected fish from vaccinated and control groups showed formation of enlarged cells as well as necrotized cells especially in spleen and kidney tissues. On the other hand, surviving fish from both vaccinated and control groups did not contained formation of enlarged cells in spleen, kidney, liver, and stomach.

RANDOMLY AMPLIFIED POLYMORPHIC DNA (RAPD) FINGERPRINTING OF SIX INDONESIAN POPULATIONS OF GIANT FRESHWATER PRAWN, Macrobrachium rosenbergii Imron Imron; Dadan Sunandar; Rini Susilowati; Rommy Suprapto; Ikhsan Khasani
Indonesian Aquaculture Journal Vol 4, No 2 (2009): (December 2009)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Indonesia is rich of giant fresh water prawn (GFP) germ plasms. Best utilization of these resources for the purpose of either aquaculture development or conservation of genetic resources requires some information on the structure and levels of their genetic diversity. This study was aimed to characterize those GFP genetic resources by applying RAPD genetic markers. Six Indonesian populations of GFP from Asahan, Barito, Ciasem, Ogan, GImacro and Papua were collected and analyzed for their genetic variation using five RAPD primers. The results showed the diversity within the populations, as revealed by the level of polymorphism, ranged from 29% to 76% while genetic divergence between populations as shown by genetic distance ranged from 0.04 to 0.50. In terms of genetic divergence, two genetically distinct groups of GFP, namely the Papua GFP in one group and the remaining five GFP populations in the other, were identified. The results also showed the presence of specific population markers that are useful for genetic identification of GFP populations. Implication of these finding with regard to breed development is discussed.

Page 6 of 34 | Total Record : 334

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Ofan Bosman

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