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Journal of Tropical Crop Science
Published by Institut Pertanian Bogor
ISSN : 23560169     EISSN : 23560177     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Tropical Crop Science is an international journal publishing research related to tropical crops. Articles in the journal focus on in-vivo and in-vitro production and production environment, breeding, seed technology, physiology, postharvest, pest and pathology of cereals, vegetables, fruits, ornamentals, medicinal and plantation crops; soil, landscape, turf and agro ecology management under tropical conditions.
Arjuna Subject : -
Articles 343 Documents
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Optimizing SCoT-PCR Parameters for Reproducible Genetic Diversity Analysis in Cassava, Rice, Maize, and Foxtail Millet Yudiansyah Yudiansyah; Ismiyanti Ismiyanti; Susilawati Susilawati; M Reza Pahlevi; Sintho Wahyuning Ardie
Journal of Tropical Crop Science Vol. 13 No. 02 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.02.494-503

Abstract

Technical optimization of PCR parameters is essential to ensure efficiency and accuracy in genetic analyses. The performance and reproducibility of Start Codon Targeted (SCoT) markers are strongly influenced by annealing temperature (Ta) and primer concentration. Raising Ta from 50 °C to 55 °C reduced both amplicon yield and reproducibility, whereas higher primer concentrations increased amplicon counts without substantially affecting reproducibility. Optimal amplification was achieved at Ta 50 °C with a primer concentration of 2.5 μM, where most primers produced high amplicon numbers with 100% reproducibility. Evaluation of 36 SCoT primers in foxtail millet identified 20 primers with full reproducibility, of which 10 were further tested across cassava, rice, and maize. Primer reproducibility varied among species, and only SCoT‑35 and SCoT-36 consistently achieved 100% reproducibility across all three crops. These results highlight that primer suitability is species‑dependent and emphasize the need for extensive primer screening in each plant species. Overall, optimizing Ta and primer concentrations, combined with careful primer selection, is essential for the reliable, efficient, and cost-effective application of SCoT markers in plant genetic diversity analysis.
The Effect of Light Intensity and Watering Intervals on the Growth and Flowering of Pentas (Pentas lanceolata) Adea Ananda Putri; Sitawati Sitawati
Journal of Tropical Crop Science Vol. 13 No. 02 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.02.457-466

Abstract

Pentas (Pentas lanceolata) is an African ornamental species widely cultivated in Indonesia for landscaping and potted plant production. However, the optimal combination of light intensity and watering interval for maximizing growth and flowering under potted conditions remains unclear. This study aimed to evaluate the interaction effects of light intensity and watering intervals on the growth and flowering of Pentas. The experiment was conducted from December 2024 to March 2025 in Sukun District, Malang, Indonesia (440 masl.), using a split-plot design with three replications. Light treatments consisted of full light (100% or ± 18,898 lux), moderate light (75% or ± 12,216 lux), and low light (50% or ± 6,122 lux), while watering intervals were applied every 3, 4, or 5 days. Data were analyzed using analysis of variance (ANOVA) followed by Tukey’s Honestly Significant Difference (HSD) test. The results showed that light availability and watering interval influenced plant growth and flowering, with responses differing between growth stages. Optimal vegetative growth, indicated by the highest shoot dry weight, was achieved under moderate light intensity (75%) combined with a 4-day watering interval. In contrast, the best flowering performance was observed under full light conditions (100%) with the same watering interval. Full light increased the chlorophyll index by 28.73% compared to low light intensity (Figure 3), while moderate light accelerated flower initiation by approximately 19 days (9.40%) (Figure 4). Although watering alone had a limited effect, its interaction with light availability was crucial in regulating overall plant performance. These findings indicate that stage-specific management of light and watering is essential for optimizing the growth and flowering of potted Pentas.
Effect of Mint and Rosemary Aromatic Oil Extracts on the Mortality of the Stored Date Pest Ephestia cautella (Walker) Saraha Ismail; Aseel Kemal Abdul Hussein
Journal of Tropical Crop Science Vol. 13 No. 02 (2026): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.13.02.574-585

Abstract

Ephestia cautella is one of the most common insect pests of stored dates, causing quantitative and qualitative losses. Therefore, this research aimed to evaluate the insecticidal effect of oil extracts from peppermint (Mentha piperita) and rosemary (Rosmarinus officinalis) against different larval instars of the date moth under laboratory conditions, to promote botanicals as a safe and environmentally friendly alternative for managing insect pests. This study was conducted in the Advanced Laboratory of Entomology at the Faculty of Education for Women, University of Kufa, during 2024-2025. The academic year showed the contact and digestive effects of both oil extracts at concentrations of 2.5%, 5%, 7.5%, and 10% on the mortality of second- and fourth instar larvae of E. cautella. The results showed high efficacy of the tested plant oil extracts in causing mortality across both larval instars. The contact effect of mint extract was slightly more effective against second-instar larvae than fourth-instar larvae across all treatments, with mortality reaching 100% in most treatments and concentrations, except at 2.5%, where it recorded 80% mortality for second-instar larvae. With rosemary extract, mortality percentages among fourth-instar larvae were higher under contact than under digestive treatment at all concentrations. Most contact treatments achieved 100% mortality, even at the 10% concentration. Digestive treatments resulted in an 80% mortality rate of second-instar larvae and a 50% mortality rate of fourth-instar larvae. These results demonstrate the potential of peppermint and rosemary oil extracts as effective botanical insecticides for controlling the date moth.

Page 35 of 35 | Total Record : 343

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