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Bioma : Berkala Ilmiah Biologi

bioma
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Published by Universitas Diponegoro

ISSN : - EISSN : 25982370 DOI : -

Core Subject : Education, Agriculture,

Agriculture, Biological Sciences & Forestry Education

BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.

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Articles 12 Documents

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Search results for , issue "Vol. 21, No 1, Tahun 2019" : 12 Documents clear

Phylogenetic Analysis Of Pigmented Marine Derived Yeast Associated With Sargassum sp. Based On Internal Transcribed Spacer (ITS) Tri Lunggani, Arina; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Karimunjawa Jepara is a region with high diversity, including the diversity of seaweed. Brown seaweed is potential marine organisms due to their ability to produce enzymes, pigments, and bioactive compounds. This ability makes brown seaweed one of the potential biological agents from the marine to be developed in the industrial field. However, most of these substances may not be produced by seaweed itself, but cooperation with microbes or even by bacteria or symbiotic fungi. Secondary metabolites which are pharmaceutical, enzyme, and cosmetic sources can be produced by microbial associan. The purpose of this study was to carry out phylogenetic analysis and morphological characterization of colonies from pigmented yeast associated with Sargassum sp. The results of the phylogenetic analysis indicate that isolates of KY 3 have 100% relative similarity with Cystobasidium oligophagum.

Variasi Warna Dan Ketinggian Sticky Trap Dengan Atraktan Methyl Eugenol Sebagai Pengikat Serangga Polinator Dan Serangga Lainnya Pada Musim Bunga Pohon Jambu Air Merah Delima Tarwotjo, Udi; Hadi, Mochamad; Rahadian, Rully
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The objective of this research was to determine the effectiveness of color and height variation of sticky trap with methyl eugenol on Insect visitting of flowers and other insect on apple water tree in Demak. Sampling of insects was conducting in one of apple water field in Demak using sticky trap with methyl eugenol attractant . There were three different colors of sticky trap and two height variation of trap placement were used. The data was analysed using descriptive analysis. Parameters observed included the number of fruit flies in each color, height of sticky trap, and the family of insects. The results showed that the insects found consist of 5 orders and 21 families. (Diptera, Hymenoptera, and Coleoptera, Lepidoptera, and Homoptera). . Most families are found in the order Diptera (8 families), Hymenoptera (6 families), Coleoptera (3 families), and Homoptera (4 families). The Diptera family consists of Tephritidae, Culicidae, Agromyzidae, Muscidae, Asillidae, Mycetophyllide, Drosophyllidae, Bombyllidae. The conclusion of this research is that the control of inseks visitting by using bright color sticky trap (yellow and white) with methyl eugenol attractant can be used as a trap of insect vistting of Diptera and other insects in the largest number of families

Isolasi dan Identifikasi Morfologi serta Biokimia Khamir Hasil Isolasi dari Buah Tomat (Lycopersicum esculentum) yang Berpotensi menghasilkan Bioetanol Anggrayeni, Yesti Tri; Wijanarka, W; Kusdiyantini, Endang
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Bioethanol can be obtained from the fermentation process by using microorganisms such as yeast. One of the factors that affect height low bioethanol is a kind of yeast, and therefore the isolation and identification of yeast need to be done in order to obtain isolates potentially producing bioetanol. Yeast can be found in various environments, especially rich sugar substrate. Yeast usually living in fruits like tomatoes. This research aims to isolation and identifies yeast from tomatoes and the growth of yeast isolates at 50% glucose concentration test. The method of isolation was performed by streak method with the four scratch quadrant technique on YGP solid media. Identification of macroscopic and microscopic morphology in colonies and cell of yeast. Biochemical identification of the growth in liquid media, the fermentation of sugars test (glucose, galactose, sucrose, maltose, lactose), as well as the growth of yeast,Â isolates in 50% glucose medium. Determination of bioethanol content is done by distillation process and the measured weight with a pycnometer. The result from isolation yeast on tomato fruits obtained nine isolates is Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9. Based on the identification of the morphology, biochemistry, as well as the growth of yeast isolates testing on 50% glucose concentrations of selected isolates Y2 alleged genus Debaryomyces sp. and is able producing ethanol of 8.7% v/v.

Produksi Protease Alkalis Termostabil Dari Aspergillus flavus DUCC- K225 Dengan Ammonium Sulfat Sebagai Sumber Nitrogen Putra, Mohammad Affan Dwica; Rukmi, MG Isworo; Pujiyanto, Sri; Mulyani, Nies S
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

ProteaseÂ is a protein hydrolytic enzyme whichÂ can be generated by a variety of microorganisms, includingÂ mold. Aspergillus flavus K225, DUCC is indigenous mold isolated from lime soil of Madura which isÂ have potential as a alkaline proteaseÂ producer. This research aims was to know the effectÂ of ammonium sulfate as nitrogen source for the production of protease enzymes by Aspergillus flavus DUCC-K225. The production of alkalineÂ protease were conducted inÂ submerged culture medium with agitation. Fermentation medium used was modification Czapeks Dox Broth containing 2% casein. Incubation is carried out for 7 days.Â The results showed that ammonium sulfate is a good source of nitrogen for growth and production of aklaine protease enzyme, which is demonstrated byÂ higher dry weight, the protease activity, the protein content and the specific activities, comparing thoseÂ on standard medium using sodium nitrate as N source.Â

Karakterisasi Senyawa Flavonoid Ekstrak Polar Daun Gamal Kultivar Lampung Utara Dan Uji Aktivitasnya Terhadap Kutu Putih Kakao (Planococcus minor, Hemiptera: Pseudococcidae) Nukmal, Nismah; Pasutri, Agata Yelin; Pratami, Gina Dania
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Cocoa is one of the export commodities that have great prospects. The quality of cocoa in Indonesia are not satisfactory, this is caused by cocoa mealybug (Planococcus minor). Gamal (Gliricidia maculata) contains flavonoid compounds which can be used as bioinsecticides. The aims of research to determine the characteristic of flavonoid compounds in gamal leaf powder which is effective for kill of cocoa mealybug P. minor. Extraction and spectroscopy analysis (UV-Vis and FTIR) were done in the Integrated Laboratory of Technology Innovation Center (ILTIC) and the bioassay was done at Zoology Laboratory of Unila. The results shows that the crude water extract of gamal leaf powder North Lampung Cultivar (NLC) more effective killed lower than purified water extract. It was indicated by value of LC50.72hours the crude water extract than purified water extract (0.11%: 0.27%). The flavonoid compounds of gamal leaf powder NLC have the characteristics of blue fluorescence in UV light Î» 254 and 366 nm. The flavonoid with the maximum peak of wavelength 310 could be grouped to flavonone with structural frame O-H, C=O carbonyl, aromatic C=C, and C-O.

Struktur Komunitas Hutan Mangrove Di Sungai Donan Kabupaten Cilacap, Jawa Tengah Ashari, Desy Puspita; Muhammad, Fuad; Utami, Sri
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Mangrove forests are plant communities in coastal areas that have high productivity. The development of the Cilacap coastal area poses a threat to the degradation of the Cilacap coastal environment. The Cilacap Coast has mangrove ecosystems that grow along the Donan River but its sustainability is threatened due to mangrove forest degradation. Mangrove forest monitoring is very important to determine the current situation due to the degradation and conversion of mangrove forests. This study aims to determine the community structure of Sungai Donan mangrove forest. Determination of research location with purposive sampling method. Mangrove vegetation sampling technique uses square plot method. Mangrove vegetation analysis uses important value index of mangrove species, diversity index, dominance index and evenness index. The results of the study there were 10 species of mangroves including Aegiceras corniculatum, Avicennia alba, Bruguierra cylindrica, B. gymnorrhiza, Nypa fruticans, Rhizophora apiculata, R. mucronata, Sonneratia alba, Hibiscus tiliaceus and Terminnalia catappa. The results showed that at each station the highest importance index was Avicennia alba in the upstream of Donan river, Alas Malang and Perhutani Banyumas Barat. The highest important value index in the Cilacap Industrial Area and the Kutawaru mangrove species are Sonneratia alba and Hibiscus tiliaceus. Sungai Donan mangrove forest community has a moderate index diversity value ranging from 1.23 to 1.78, high evenness ranges from 0.89 to 0.97, and low dominance index values range from 0.18 to 0.34.

Pengaruh Penyiraman Air Cucian Beras Fermentasi Satu Hari Dan Fermentasi Lima Belas Hari Terhadap Kadar Pigmen Fotosintetik Dan Pertumbuhan Vegetatif Tanaman Sawi Hijau (Brassica juncea L.) Fadilah, Aprilia Nurul; Darmanti, Sri; Haryanti, Sri
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Mustard plants are a type of vegetable favored by most people. Demand formustard plants continues to increase along with the increasing population and awareness of nutritional needs, but there are obstacles in increasing the production. This is due to the lack of nutrients needed by the mustard plant for its growth. One effort to increase the production of mustard plants is by adding nutrients through fertilization. Fertilizers are divided into two types, namely organic and inorganic fertilizers. However, long-term use of inorganic fertilizers can reduce soil quality and environmental health. One alternative to overcome these problems is to use liquid organic fertilizer in the form of fermented rice washing water. This study aims to determine the effect of 1-day fermented rice washing water and 15-days fermented rice washing water at different concentrations on photosynthetic pigment content and vegetative growth of green mustard plants and find out the best dose of rice washing water for the growth of green mustard plants. The study used a Completely Randomized Design (CRD) of 1 factor with 5 treatments. Each treatment with 4 replications with ordinary water as a control, 1-day fermented rice washing water with the concentration of 50% and 100%, and 15-days of fermented rice washing water with the concentration of 50%, and 100%. The results showed that the watering on 1-day fermented rice washing water with the concentration of 50% and 100% and 15-days of fermented rice washing water with a concentration of 50% had no effect on all parameters but the watering of 15-days fermented rice washing water with a concentration of 100% had an effect on increasing the plant height, number of leaves, leaf area, plant fresh weight, and plant dry weight. The growth of mustard green plants (Brassica juncea L) is best obtained in the treatment of 15-days fermented rice washing water with a concentration of 100%.

Phytochemicals Screening and Effectiveness of Free Radical Inhibitors of Garlic (Allium sativum L.) Ethanol Extract from Timor Island Priska, Melania; Peni, Natalia; Carvallo, Ludovicus
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

The purpose of the study was to determine the content of secondary metabolites and the effectiveness of garlic (Allium sativum L.) ethanol extract from Timor island which has the potential to inhibit free radicals. The method used for testing secondary metabolites is phytochemical screening using color reagents. The effectiveness of free radical inhibitors from the ethanol extract of garlic (Allium sativum L.) from Timor island was carried out in two steps are: 1.) Determination of DPPH wavelength (Î») maximum and 2.) Measurement of antioxidant activity using the DPPH method. The results showed the ethanol extract of garlic (Allium sativum L.) from Timor Island contained secondary metabolites of flavonoids, phenols, and terpenoids. The ethanol extract of garlic (Allium sativum L.) from Timor Island also has strongest effectiveness in inhibiting free radicals, with the acquisition of IC50 values <50 ppm which is equal to 9,729 ppm.

Aktivitas Antibakteri Ekstrak Etanol dan Etil Asetat Batang Tanaman Brotowali (Tinospora crispa, L. Miers) terhadap Bakteri Escherichia coli Enteropatogenik (EPEC) Penyebab Penyakit Diare Fathmah, Ema Nuzula; Pujiyanto, Sri; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Diarrheal diseases is an endemic desease in Indonesia and potential desease of Kejadian Luar Biasa (KLB) which often leads to death. Enteropatogenic Escherichia coli (EPEC) is one of pathogenic bacteria which can cause diarrhea. Brotowali plant (Tinospora crispa, L. Miers) is one of traditional medicinal plants which is widely used to treat various diseases and and relatively have no side effects than chemical drugs. he purpose of this research is to know the antibacterial activity of Brotowali (Tinospora crispa, L. Miers) stems ethanol and ethyl acetate extract in inhibiting the growth of EPEC bacteria causing diarrheal disease. Brotowali extract was obtained by maceration method using ethanol and ethyl acetate solvent. The condensed extract obtained was dissolved in DMSO 100% until the concentration of the extract to 20%; 40% and 60%. Test of antibacterial activity used diffusion method using paperdisk. The ethanol and ethyl acetate extract of brotowali stems were each tested on EPEC bacteria. The results in this study are the inhibition zone on ethanol and ethyl acetate extract stem of brotowali plant to EPEC bacteria. The antibacterial activity of brotowali stem ethanol extract at 60% concentration showed the best effect compared to the concentration of 20% and 40%. The antibacterial activity of ethyl acetate extract of brotowali stem was as good at concentration of 20%; 40% and 60%. The antibacterial activity of brotowali stem ethyl acetate extract showed better results than brotowali stem ethanol extract

Kelimpahan Dan Keragaman Serangga OPT (Organisme Pengganggu Tanaman) dan Musuh Alaminya pada Tanaman Jagung dan Padi dengan Sistem Rotasi Tanaman Ervianna, Ana Rita; Hadi, Mochamad; Rahadian, Rully
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Insects in agricultural ecosystem can play a role as pests or natural enemies. The abundance and diversity of insect pests and natural enemies are interconnected because insect pests are food for natural enemy insects. The aims of the study were to compare the abundance and diversity of pest insects and natural enemies of corn and rice plant, and to determine the similarity of pest insects and natural enemies of corn and rice plant using crop rotation systems. The sampling has performed four times i.e, in the vegetative phase of corn, generative corn, vegetative rice, and generative rice. The sampling method used sweep net method. The results showed that the number of individual pest insects and natural enemies of each growth phase of corn plants were lower than rice plants. The number of species pest insect and natural enemies of each growth phase of corn plants were lower than rice plants. In addition the diversity of pest insect of each growth phase of corn plants was higher than rice plants while the diversity of natural enemies in each growth phase of corn plants was lower than rice plants. The diversity index of pest insects and natural enemies of each growth phase in corn plants and rice plants was in the medium category. The presence of pest insects andÂ natural enemies in each growth phase of corn and rice plants shows the species in the two compared ecosystems were different.

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