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INDONESIA
Teknologi Indonesia
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 01261533     EISSN : 23031913     DOI : -
Core Subject : Science,
Computer Science & IT
JTI is a journal in the Departement of Engineering Sciences - Indonesian Institute of Sciences (LIPI). JTI has policy to publish a new and original research paper or a review paper in The scope of Technology. JTI publishes two issues per year. The journal has been registered with printed-ISSN number as 0126-1533 and electronic ISSN number as 2303-1913.
Arjuna Subject : -
Articles 6 Documents
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Search results for , issue " Vol 40, No 1 (2017)" : 6 Documents clear
VARIATION IN POINT MUTATIONS OF L -ARABINOSE ISOMERASE Thermotoga thermarum (TTAI) THROUGH THE ANALYSIS OF ENZYME STRUCTURE AND DOCKING SIMULATION Syahputra, Gita; Kusharyoto, Wien
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

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Abstract

D-tagatose is one of the products of L-arabinose isomerase which can be applied to food, cosmetics, and health. D-tagatose produced by L-AI has a low quantity. L-AI enzyme from thermophilic bacteria as Thermotoga thermarum (TTAI) can produce higher quantity of D-tagatose than mesophilic bacteria. Point mutation can increase L-AI activity. The analysis of enzyme structure and docking simulation can determine the variation in point mutations. Based on analysis of enzyme structure and docking simulation, TTAI has 494 amino acids compiled by 17 of α-helix dan 18 of β-strand. The analysis of TTAI structure get several active side residues Gln16, Leu18, Tyr19, Phe81, Gln125, His126,Met183, Phe273, Glu300, Glu327, Tyr329, His344, Met345, Ile366, His442, His443. The docking simulation suggested that the residues for binding D-tagatose are Trp422 and Tyr331. Two point mutations, i.e. M183A and F273L, are recommended based on the analysis of TTAI structure, homology structure, residues, and docking simulation. This variation in mutation can be used for further research in vitro. 
GC/MS PROFILING AND EVALUATION OF ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF BAYUR (Pterospermum javanicum) BARK EXTRACT Praptiwi, Praptiwi; Fathoni, Ahmad
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

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Abstract

This study aims to identify the chemical compounds of ethyl acetate extract of the bark of bayur (Pterospermum javanicum) and to evaluate its antibacterial activity and potential antioxidant. The estimation of total flavonoid was done by spectrophotometry method. The chemical compounds were analyzed by gas chromatography-mass spectrometry (GC-MS). The antibacterial assay against Staphylococcus aureus Ina-CC B5 and Escherichia coli Ina-CC B4 was done by TLC-bioautographic method, whereas the potential antioxidant was determined by DPPH radical scavenging assay. Meanwhile, its minimum inhibitory concentration (MIC) value and IC50 of the extract were done on microplate 96 well by serial microdilution method. GC/MS analysis revealed the presence of 31 identified chemical compounds in the extract with total flavonoid was 625 mg CE/gram extract. The MIC values against E.coli Ina-CC B5 and S. aureus Ina-CC B4 were >256 ug/ml. The IC50 from DPPH radical scavenging assay was 48.92 ppm indicating that ethyl acetate extract of the bark of bayur is potent antioxidant.
THE SUBSURFACE STRUCTURE INVESTIGATION OF DIENG PLATEUAU, CENTRAL JAVA, INDONESIA BY USING THE MAGNETOTELLURIC DATA Gaffar, Eddy Zulkarnaini
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

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Abstract

Dieng Plateau area has a geothermal energy power station with a capacity of 60 MW. Research Center for Geotechnology, Indonesian Institute of Sciences (LIPI) tried to expand the productive areas in Dieng to other prospective areas around Dieng Plateau using the Magnetotelluric method. Magnetotelluric measurement was done by taking path stretching from east to west and from north to south comprising of 24 locations, each with a distance of 2 to 4 km. The tools used are Phoennix MTU-5A by using two kinds of coils, namely MTC-30 for high frequency 1,000–10.000 Hz and MTC-50 for low frequencies from 0.00001 to 400 Hz. Three lines for the two-dimensional cross section were made. From the three cross-sections, there are rocks with resistivity value of ≤32 Ωm interpreted as soft rock or alteration rock and Dieng volcanic rocks as cap rock. Under the cap rock, there are rocks with resistivity value between 64–1.000 Ωm with thickness of about 1,000–2,000 meters which are interpreted as volcanic clastic rocks and lava from Jembangan, Damar, and Ligung Formation as reservoir rocks. In the very bottom rock, there are rocks with resistivity value of ≥1,000 Ωm interpreted as igneous rock which still contain heat from the Quaternary intrusion that are not exposed at the surface and the Kumbang Formation consists of andesite and basalt lava. The developing structure is classified as normal fault structures as seen on all of the line that make the reservoir trap better. On the western region, namely Wanayasa area and the north west area of Mangunan, there were rocks with the same system and structure as found on the eastern area (Dieng prospect area). Therefore, in addition to current productive Dieng area, there are also other prospective areas in Wanayasa and Mangunan area.
EFFECT OF SINTERING TEMPERATURE ON THE ROLLED COPPER-SHEATHED MONOFILAMENT MgB2 SUPERCONDUCTING WIRE Herbirowo, Satrio; Imaduddin, Agung; Sofyan, Nofrijon; Yuwono, A.H
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

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Abstract

The manufacture of low temperature superconductor (LTS) MgB2 with relatively high critical temperature (Tc = 39K) wire fabricated by powder-in-tube (PIT) is a multi-step process. The main difficulty is that the value of Tc superconductor wire is determined by various factors. MgB2 superconductor has been developed for possible utilization in various practical applications, such as Magnetic Resonance Imaging. The fabrication process of 2 mm rolled-copper sheath monofilament superconductor MgB2 wire using mechanical deformation process, including rolling and drawing, has been carried out. Starting by inserting powders, containing nominal composition of MgB2 into Cu tube with inner ø of 4 mm and outer ø of 6 mm, then they were rolled and drawn into wires (ø 2 mm), subsequently. The process of copper tube rolling was subsequently repeated in order to obtain three samples using added 5% CNT and 10% CNT to the pure MgB2, then were heat-treated at 800°C for 2 hours respectively. The writers evaluated the doping effect of two types multiwall carbon nanotube (MWCNT) with different aspect ratios on MgB2/Cu monofilament wires. Surface morphology was analyzed with SEM, crystal structure of MgB2 with XRD, whereas temperature dependence resistivity with four-point probe Cryogenic Magnet. SEM-EDS images showed that without addition of CNT, the crystal of MgB2 form dispersed uniformly in wire, but when added with 5% and 10% of CNT, the particle dispersed morphology was observed due to substitution of atom C into atom B. In contrast with pure MgB2, however, the pores increased as the CNTs doped increased of up to 5 wt%, makes it hard to identify the critical temperature. The temperature dependence of resistivity results showed that the critical temperatures were detected and kept shifting toward high 40 K with the addition of 10 wt% CNT. XRD analysis showed that some Mg may react with CNT to form MgB4 and graphite. Mg may react with oxygen to form MgO because the Cu sheath is not fully sealed and vacumed.
LACTIC ACID AND HYDROGEN PRODUCTION FROM MICROALGAL BLOOM BIOMASSES Susilaningsih, Dwi -; Harahap, Puspita Sari; Farida, Hilda -; Rahman, Delicia Yunita
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

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Abstract

Microalgal bloom occurrence is rapidly increasing in a very short time in a specific condition and dominating its habitat. Therefore, blooming often occurs in water ecosystems, from freshwater to brackish and marine. Although there is no officially acknowledged threshold concentration level, the amount of algae cells is estimated reaching hundreds or even thousands cells per mililiter, depending on the species and types. Algae blooming is considered harmful because it gives such a big impact varies on water ecosystems, depending on the species involved, the environment where they are found, and their mechanism metabolism. The hazardous algae poison causes severe effect to various species, such as mammals and other sea creatures, with each presenting specific toxicity in decreasing motor development, immunology, neurological capacity, and reproduction capacity.In this report, the tropical algal bloom biomasses were used for fermentation substrate as substituent of carbon sources in purpose to produce a useful compound of lactic acid. Lactic acid is base material used in forming polylactic acid (PLA) polymerase that is known as basic material for biohydrogen precursor. The lactate bacteria were isolated from local tropical fruit.The result showed all microalgal biomasses converted successfully into lactic acid for 68–92% based on total carbohydrates conversion into lactic acid compounds. Algal bloom biomasses contained mostly of carbohydrate complexes (60–70% of dry weight), protein (10–20% of dry weight), lipid (20–30% of dry weight), and other compounds (5–10% of dry weight). The fermentation result showed that various microalgal blooms biomasses were successfully converted into lactic acid ranging from 58–92% based on total carbohydrates conversion into lactates. Furthermore, the obtained lactic acid was converted into polylactic acid through direct polycondensation process with average result of 60–84% formation unit base on lactic acid weight. Therefore, lactic acid was also successfully converted into hydrogen gas using fermentation process with efficient value from 78–90% unit based on the weight of lactic acid molecules. All these results showed that the unuseful algal biomass could be utilized as a substrate or precursor of fermentation process in term of biodegradable material and energy production, and also lactic acid is an applicable compound in forming polymers and energy.
OPTIMIZATION PRODUCTION OF XYLANASE BY Bacillus pumilus IN EMPTY FRUIT BUNCH USING 3 L BIOREACTOR Wijaya, Hans; Thontowi, Ahmad; Yopi, Yopi
Teknologi Indonesia Vol 40, No 1 (2017)
Publisher : LIPI Press

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Abstract

Indigenous biomasses, such as empty fruit bunch, contain high xylan and are useful to produce raw xylanase, where this biomasses are very abundant in Indonesia. The focus of this research is to scale up the fermentation product into 3 litter feeds and to record the highest enzyme activity. Fermentation process has been done by using stirred fermentor MBI Winpact One Fermentation System. The isolate marine bacterium Bacillus pumilus has been used to degrade empty fruit bunch to produce raw xylanase. The result for different agitation speed showed that at 150 rpm has the highest xylanase activity. The effect of different aeration flows gives at a rate of 4 VVM achieved the highest enzyme activity and equal to 7.61 U/mL with cell growth equal to 2.06. The Sigma Antifoam C has been successfully being used as defoamer in the production fermentor and can be tolerated by Bacillus pumilus. The comparisons of different medium to generate different enzyme by Bacillus pumilus has achieved xylanase, mannanase, and cellulase, where xylanase has the highest enzyme activity among all of the enzymes produced.

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