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Scripta Biologica
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 23553138     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.
Arjuna Subject : -
Articles 11 Documents
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Search results for , issue "Vol 5, No 3 (2018)" : 11 Documents clear
PHYTATE ACTIVITY OF THERMOPHILIC BACTERIA FROM SULILI HOT SPRINGS IN PINRANG DISTRICT SOUTH SULAWESI Hafsan Hafsan; Nurjannah Nurjannah; Cut Muthiadin; Isna Rasdianah Aziz; Ahyar Ahmad; Laily Agustina; Asmuddin Nasir
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.819

Abstract

This study aimed to determine the optimum temperature and pH of the phytase activity produced by isolate of thermophilic bacteria from Sulili hot springs in the district Pinrang south sulawesi. This is a descriptive research, that measure of phytase activity by treated the variation of temperature and pH. The various of temperature has eight levels ie 20; 30; 40; 50; 60; 70; 80; and 90oC, the pH treatments consists of eight levels ie 2; 3; 4; 5; 6; 7; 8 and 9. Phytase activity was determined by converting the Na-phytate absorbance values produced by phytase enzyme against Na-phytate standard curve. One unit of phytate enzyme activity is defined as the amount of enzyme capable of hydrolysed Na-phytate and produce a peptide which is equivalent to one mole of tyrosine per minute under the conditions of analysis. The results of studies have shown that there is a significant effect of temperature on the phytase activity of Bacillus coagulans, thermophilic bacteria from Sulili hot springs, whereas no significant effect of pH treatment. Optimum phytase activity of B. coagulans are at 60°C and pH 7 ie 6.11 units/ mL.
HUBUNGAN SIKLUS MENSTRUASI DENGAN KADAR KALSIUM GINGIVAL CREVICULAR FLUID PENDERITA PERIODONTITIS KRONIS Amilia Ramadhani; Ziyada Salisa; Fani Tuti Handayani
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.827

Abstract

Background: Periodontal disease is commonly caused by Porphyromonas gingivalis that released lipopolysaccharide endotoxin (LPS). The stimulation of pro-inflammatory cytokines secretion in periodontal tissue is not only derived from pathogenic bacteria. Various genetic conditions and systemic conditions are also being the trigger factor. One of systemic factor is sex hormones such as estrogen and progesterone. Both of these hormones fluctuate during the menstrual cycle. Purpose: The purpose was to determine the relationship of menstrual cycle based on ovarian cycle with calcium gingival crevicular fluid (GCF) levels in chronic periodontitis patient. Methode: This research was analytic observation. The samples in this reasearch were 9 women who aged 17-45 years with a diagnosis of chronic periodontitis and had a normal menstrual cycle (21-35 days). All subjects were calculated menstrual cycle and determined the phase of follicular, ovulation, and luteal. Clinical examination and GCF sampling were performed at each phase. Measurement of calcium GCF level was done using spectrophotometer. Result: During follicular phase of menstruation cycle, the periodontal index arises until 1,62, meanwhile the highest calcium concentration occurred in ovulation phase of menstruation cycle. Linier regression correlation test showed no significant correlation between periodontal index, calcium concentration and menstruation cycle. Conclusion: Fluctuation in women’s sexuality hormones during menstruation cycle do not related to alveolar bone damage and GCF’s calcium concentration.
KONDISI EKOSISTEM TERUMBU KARANG DI LOKASI TIATIKI DAN NON-TIAITIKI, KAMPUNG TABLANUSU DISTRIK DEPAPRE KABUPATEN JAYAPURA Yunus Pajanjan Paulangan
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.688

Abstract

Depapre Bay has large potential fisheries resources and very diverse that are still managed by the Tiaitiki system. Ironically, destructive fishing activities still occur. The coral ecosystems in Depapre Bay are still relatively under-studied mainly related to sustainable management. Condition of coral reef and reef fishes are the base aspect in management approach. The purpose of this research is to analyze coral reef and reef fishes condition of Tiatiki area and outside of Tiaitiki. The method used in this research is Point Intercept Transect (PIT) to identify coral condition, and Underwater Visual Cencus Method (UVC) for reef fishes observation. The results showed that lifeform conditions at 3-5 m of Tiaitiki area were "40,67% was moderate, dominated by Coral Branching, Flassy Micro Alga dan Coral Foliose; and  at 10-13 m of Tiaitiki area were 50,00% was “good”. Lifeform conditions at 3-5 m of non-Tiaitiki area were "48,67% was moderate, dominated by Coral Branching, Coral Massive dan Flassy Micro Alga; and  at 10-13 m of non-Tiaitiki area were 60,67% was “good”, dominated by Coral Branching, Acropora Branching, Acropora Tabulate, Acropora Encrusting dan Coral Foliose. In addition, at the all areas found rubbles is quite high at about 8-16,67%. Abundance of reef fishes in both areas is relatively the same, where in Tiaitiki area that are 372 individual at 3-5 m of depth and 114 individual at 10-13 m of depth, and Non-Tiaitiki area that are 262 individual at 3-5 m and 215 individual at 10-13 m.
KEPERCAYAAN LOKAL DAN KONSERVASI: TITIK TEMU ANTARA STARTEGI TRADISIONAL DAN MODERN DALAM KONSERVASI DAN PENGELOLAAN SUMBERDAYA DI PULAU SUMBA Mohammad Fathi Royyani; Oscar Efendy; Andria Agusta
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.703

Abstract

Kepercayaan lokal adalah salah satu jembatan yang menghubungkan antara pengetahuan yang ada di masyarakat dengan management pengelolaan kawasan konservasi, melalui Sacred site yang terbukti masih tetap lestari dan dijaga secara kolektif. Sacred site adalah wujud dari ekspresi kepercayaan lokal terhadap kekuatan adikodrati. Di tempat ini, jatidiri dan identitas kepercayaan ditambatkan. Mempertimbangkan efektifitas dalam menjaga kawasan maka negara, melalui aktor yang ada di dalamnya, mengadopsi system tersebut untuk diterapkan dalam management lingkungan. Artikel ini akan menunjukkan bahwa kepercayaan tradisional masyarakat dalam mengelola lingkungan bisa digunakan sebagai optik dan jembatan dalam melihat dan mengatasi dikotomi antara modern-tradisional dan alam-manusia. Tidak ada yang tradisional dan tidak ada yang modern.
ANALYSIS OF GENETIC VARIATION MUTANT SOYBEAN (Glycine max. L.MERR) GAMMA RAYS AND EMS (ETHYL METHANE SULFONATE) INDUCTION THROUGH ISSR MOLECULAR MARKERS Evika Sandi Savitri
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.874

Abstract

Mutation induction in soybeans was carried out to develop high genetic diversity as a basis for plant breeding to get superior varieties.  Mutation induction can be done using physically mutagen gamma rays and chemically using EMS mutagens.  This study aims to determine the effectiveness of chemical mutagen (EMS) and physical mutagen (gamma rays) in the induction of genetic diversity in soybeans. Mutation detection can be done with molecular markers to characterize plant genetic diversity. In this study, ISSR molecular markers were used consisting of 4 primers. The method of mutation induction used EMS and gamma rays treatment. The results of the primary PCR amplification of ISSR2, ISSR3 and UBC888 showed 100% polymorphism. The treatment of mutation induction using EMS chemical agents showed a higher degree of polymorphism compared to gamma rays treatment
KAJIAN ETNOBOTANI PADA TRADISI PERNIKAHAN ADAT KERATON SURAKARTA, PROVINSI JAWA TENGAH Eka Supriyati
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.758

Abstract

Marriage is the emotional bond between man and woman to form a happy family. Marriage tradition including their ceremonial also conducted in areas such as the Keraton Surakarta. The ceremonial in Keraton Surakarta Central Java Province has been known as a basic pattern of tradition marriage by Javanese. Various types of plants are utilized in marriage tradition. The utilization of plants by community in particular areas is studied through the scince of ethnobotay. The purpose of this study is to know the wedding ceremonial tradition of Keraton Surakarta Indonesia in terms of the use of the plants and their meanings. This research used descriptive method with interviews. The sampling used purposive random sampling on servants in Keraton Surakarta and locals near Keraton. The result of the research shows the tradition of Surakarta traditional marriage utilizing various plants around it with a noble and positive meaning. The use of plants at each stage of marriage is different and has defferent meaning.
HUBUNGAN ANTARA KADAR UREUM, KREATININ DAN KLIRENS KREATININ DENGAN PROTEINURIA PADA PENDERITA DIABETES MELLITUS Vitasari Indriani
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.726

Abstract

Pemeriksaan ureum, kreatinin dan klirens kreatinin adalah pemeriksaan untuk monitoring fungsi ginjal seseorang. Pemeriksaan ini dapat dilakukan pada laboratorium pratama dengan akses yang terjangkau. Pemeriksaan albumin urin sebagai petanda dini dari komplikasi mikrovaskuler pada DM. albuminuria relative mahal dan tidak semua laboratorium dapat melaksanakan pemeriksaan ini, sehingga penelitian ini diharapkan dapat memberikan alternatif deteksi komplikasi nefropati diabetikum pada penderita DM. Membuktikan adanya hubungan ureum, kreatinin dan klirens kreatinin dengan proteinuria pada penderita DM. Harapannya pemeriksaan fungsi ginjal dapat dilaksanakan rutin pada pusat pelayanan kesehatan 1 untuk mendeteksi dini komplikasi nefropati pada penderita Diabetes Mellitus. Desain observasional analitik dengan pendekatan belah lintang (cross sectional). Responden 35 orang DM dilakukan pemeriksaan ureum dan kreatinin metoda enzimatik, Klirens kreatinin dengan perhitungan cockroft gault formula dan proteinuria dengan pemeriksaan albumin urin metoda immuno chromatografi. Hubungan antar variabel dianalisis dengan uji korelasi Pearson. Terdapat hubungan antara kadar ureum, kreatinin, klirens kreatinin dengan proteinuria (p=0,298;0,386;0,382).Komplikasi DM mikrovaskuler yang terjadi akibat kerusakan glomerulus menyebabkan sejumlah protein darah diekskresikan ke dalam urin secara abnormal. Protein utama yang diekskresikan adalah albumin. Peningkatan kadar albumin dalam urin merupakan tanda awal adanya kerusakan ginjal oleh karena diabetes. Terdapat hubungan sinifikan antara ureum, kreatinin dan klirens kreatinin dengan proteinuria pada pasien Diabetes Mellitus.
STUDI PENYEBARAN SPASIAL PALEM DI HUTAN LINDUNG GUNUNG SLAMET, JAWA TENGAH [Study on Palm spatial distribution at Gunung Slamet Nature Forest, Central Java] Insani Widya Astuti; Siswoyo Siswoyo; Himmah Rustiami
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.825

Abstract

Ecological studies and the spatial distribution of two forest ecosystem, natural forest and mixed forest, on Mount Slamet, Baturaden, Central Java has been done. There are 12 species of palms in natural forests, Calamus asperimus, C. heteroideus, C. javensis, Calamus sp. 1, Calamus sp. 2, Caryota maxima, C. mitis, Daemonorops rubra, Pinanga coronata, P. javana, Plectocomia elongata, and Salacca zalacca. While in the mixed forest were found six species of palm that were C. asperimus, C. ciliaris, Caryota maxima, Pinanga coronata, P. javana, and Plectocomia elongata. The species that had highest Importance Value Index (IVI) in the two types of ecosystems were Pinanga coronata. Pattern of its distribution both in natural and mixed forests have Morisita index value> 0. This value means that palm has a clustered distribution pattern. The highest spatial distribution of natural palm forest was found in 8-15% slopee class and height class 1,100 - 1200 m asl. Whereas for mixed forest it is found mostly in slopee class 8-15% and height class 900 - 1,000 m asl.
KEANEKARAGAMAN KEPITING PADA EKOSISTEM MANGROVE DI PERAIRAN NATUNA, KEPULAUAN RIAU Ernawati Widyastuti
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.702

Abstract

ABSTRACTThis study aims to determine the diversity of crabs in the mangrove ecosystems in Natuna waters, Riau Islands. The study was conducted on 23 October to 5 November 2014. The crabs were collected from eight research stations using random method, by putting a transect quadrant of 1 x 1 m2 on an area of 10 x 10 m2 area at each mangrove observation station. Results obtained 274 individuals, from 21 species, 11 genera and 8 families. Crabs of the family Sesarmidae most diverse with 8 specie: Clistocoeloma sp., Nanosesarma edamensis, Parasesarma leptosoma, Parasesarma sp1., Perisesarma cf. brevicristatum, Perisesarma semperi, Perisesarma sp1. and Sarmatium crassum. Based on quantitative analysis, the index value of diversity ranged from 0.932 – 2.028, with the highest value obtained at Cemaga Utara (St. NTNM02). The eveness index  in the range of 0.579 - 0.881 with the highest eveness at Cemaga Selatan (St. NTNM01).
KONDISI FITOPLANKTON DAN KUALITAS AIR PADA KEDALAMAN PERAIRAN BERBEDA DI PERAIRAN MALUKU Riris Aryawati; Nurul Fitriya
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.748

Abstract

Fitoplankton di laut berperan sebagai pembentuk dasar dari rantai makanan melalui produksi primer. Keberadaan fitoplankton sangat terkait dengan faktor fisika kimia perairan. Umumnya kondisi lingkungan perairan memiliki perbedaan konsentrasi di wilayah permukaan dan di perairan yang lebih dalam. Penelitian  ini bertujuan untuk  mengetahui dinamika planktonik di sekitar wilayah penelitian meliputi kelimpahan, dan komposisi fitoplankton serta kondisi fisika kimia perairan di wilayah perairan permukaan dan termoklin. Penelitian dilakukan pada bulan Oktober 2015, pada delapam lokasi penelitian. Sampling plankton dilakukan pada 2 kolom air yang berbeda, yaitu di kedalaman permukaan dan pada lapisan termoklin. Air sampel diambil dengan menggunakan Rosette sampler, kemudian disaring dengan menggunakan hand plankton net ukuran mata jaring 20 µm. Filtrat yang terkumpul di bucket lalu dimasukkan ke dalam botol sampel 250 ml dan difiksasi dengan formaldehyde 4%. Hasil penelitian menunjukkan bahwa di perairan Maluku ditemukan Bacillariophyceae (14 marga) dan Dinophyceae (6 marga). Terdapat kondisi parameter perairan yang berbeda pada kedalaman yang berbeda, khususnya untuk nitrat, fosfat, silikat, oksigen terlarut (DO) dan pH. Nitrat, fosfat dan silikat  memiliki nilai-nilai yang lebih tinggi pada kedalaman termoklin dibandingkan di perairan permukaan, sedangkan DO dan pH memiliki nilai yang lebih tinggi pada perairan permukaan dibandingkan kedalaman termoklin.  Kata Kunci: Fitoplankton, kualitas air, permukaan perairan, kedalaman termoklin, perairan Maluku.

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