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Journal of Aquaculture and Fish Health
Published by Universitas Airlangga
ISSN : 23017309     EISSN : 25280864     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The Journal of Aquaculture And Fish Health (JAFH) has an objective to publish and provide high-quality scientific contributions to the field of fisheries. These contributions came from innovative researches that encourage science and technology development in the field of fisheries and marine science on a national and international scale. This journal serves as a communication medium for researchers, academics, students, and communities.
Arjuna Subject : -
Articles 359 Documents
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DNA Barcoding of Tenualosa toli from Estuarine Waters of Sambas and Mempawah, West Kalimantan, Indonesia Rizal Akbar Hutagalung; Yudha Perdana Putra; Sadri; Syarif Iwan Taruna Alkadrie; Andrian Saputra; Enjang Hernandi Hidayat; Moh Rezha Sabda Firdaus; Sj Djunaidi; Yuda Saniswan; Eko Rahmanza Putra
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.82887

Abstract

Tenualosa toli is an economically important estuarine fish in West Kalimantan; however, molecular reference data for accurate species identification remain limited. This study applied a DNA barcoding approach based on the mitochondrial cytochrome c oxidase subunit I (COI) gene to identify specimens collected from two estuaries, Mempawah and Sambas. Four samples were successfully amplified, yielding COI fragments of approximately 650–700 bp with consistent sequence lengths (682–686 bp) and no insertions, deletions, or stop codons. BLAST analysis showed high similarity to reference T. toli sequences (99.84–100%), confirming species identity. Sequence comparison revealed very limited nucleotide variation, with three samples being identical and one sample exhibiting 1–2 synonymous substitutions (<0.3%). Phylogenetic reconstruction using the neighbour-joining method grouped all samples within a single T. toli clade and clearly separated them from other Tenualosa species used as references. Given the limited sample size and the use of a single mitochondrial marker, these findings should be interpreted strictly as species-level confirmation and as an initial molecular reference rather than as evidence of population structure. The results highlight the utility of DNA barcoding for species verification and provide baseline molecular data to support future genetic studies and fisheries monitoring of T. toli in West Kalimantan.
Assessment of Bacteria in Panulirus ornatus and Panulirus homarus Lobsters Infected with Milky Disease in Floating Net Cage Culture Media Nunik Cokrowati; Muhammad Junaidi; Muhammad Marzuki; Muhammad Sumsanto; Aprililianti
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.83061

Abstract

West Nusa Tenggara consists of the islands of Sumbawa and Lombok. It has lobster resources in the waters of both islands. Lobster production in West Nusa Tenggara comes from wild catches and aquaculture. Lobster production in Lombok reaches more than 150 tons per year, including the species Panulirus Homarus and Panulirus ornatus. Lobster aquaculture in Lombok is carried out using a floating net cage system. The challenge in lobster aquaculture is the emergence of milky disease. This disease is caused by Rickettsia-like organisms (RLO) bacteria that attack lobster body tissue, especially in the hemolymph. The impact of milky disease is very significant, especially on lobster aquaculture productivity. The purpose of this study was to analyze the abundance and types of bacteria in Panulirus Homarus and Panulirus ornatus lobsters infected with milky disease and in the seawater used for cultivation. Bacterial assessment of lobsters infected with Milky Disease is necessary to identify the bacteria causing MHD-SL and the prevalence of lobsters infected with MHD-SL. This bacterial assessment was conducted on seawater used for lobster cultivation in lobster net cages and on the meat of lobsters infected with MHD-SL. The results of the assessment can be used to determine preventive measures that can be taken to prevent MHD-SL infection. The advantage of the solution offered in this study is that bacterial assessment is carried out on seawater used for cultivation in floating net cages and bacterial analysis is also carried out on lobsters infected with MHD-SL. This research will be conducted at the floating net cages of PT. Pasopati Indo Kreasi in the waters of Gili Gede Sekotong, the University of Mataram fish health laboratory, and the Lombok Marine Aquaculture Testing Laboratory. The research method used is an experimental method conducted at sea and in the laboratory. The expected research results are the abundance and types of bacteria in lobsters infected with milky disease and in the seawater at the cultivation site.
Optimization of Thalassiosira sp. Growth in Mixotrophic Culture by Adding Sucrose and Indole-3-Acetic Acid (IAA): Optimization of Thalassiosira sp. Growth in Mixotrophic Culture by Adding Sucrose and Indole-3-Acetic Acid (IAA) Talita Shofa Adestia; Anargya Pradipa Tristanandana; Muhammad Ridzky Adhory; Ichsan Achmad Fauzi; Muhammad Agus Suprayudi; Julie Ekasari
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.85600

Abstract

Thalassiosira sp. is a significant marine diatom utilized as live feed in aquaculture; however, its culture productivity frequently remains below optimal levels. The present study evaluated the growth performance and culture quality of Thalassiosira sp. under two parallel cultivation strategies: a mixotrophic system supplemented with sucrose and a system supplemented with Indole-3-Acetic Acid (IAA). Both cultivation schemes were maintained for seven days under identical environmental conditions. Sucrose was administered at concentrations ranging from 0.25 to 1 g/L, while IAA was applied at 2.5 to 10 mg/L. Cell density, specific growth rate, dry biomass, and total Vibrio count were measured. Sucrose supplementation, particularly at 1 g/L, resulted in increased growth and biomass accumulation but was accompanied by elevated Vibrio abundance. IAA supplementation also promoted growth and biomass production, with the most pronounced effect observed at 10 mg/L, although it did not reduce bacterial proliferation. These findings indicate that both sucrose and IAA can enhance Thalassiosira sp. culture productivity under controlled conditions, but effective microbial management remains essential for practical implementation.
Sex Ratio, Size at First Maturity, and Egg Diameter of Yellow-finned medaka fish in Lake Towuti, Indonesia, as a Basis for Domestication Nursyahran Syahran; Jayadi Jayadi; Andi Yusuf; Heriansah
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.85722

Abstract

Yellow-finned medaka fish (Oryzias profundicola) is an endemic species in Lake Towuti and is often consumed by people around the lake. Despite the potential of this species, the problem of over-exploitation has led to a significant decrease in its population. Therefore, this study aims to analyze the sex ratio, first gonad maturity size, and egg diameter of yellow-finned medaka from Lake Towuti. Fish samples were caught using seser nets, and data were collected from January to June 2022 at stations located in Tanjung Timbala, Tanjung Lengkobutanga, Tanjung Bakara, Tanjung Saone, and Tanjung Tominanga. The sex of each fish was identified through the examination of body morphology and gonad. Measurement of egg diameter was carried out at gonad maturity levels III and IV, and a total of 200 eggs were observed at each station per month. The total length and total weight of samples were then measured. Data on sex ratio, size at first gonad maturity, and egg diameter were analyzed using several equations. The results showed that the sex ratio of yellow-finned medaka in Lake Towuti was balanced. The average size range at first gonad maturity of male and female fish was 32.65-39.96 mm and 25.82-33.08 mm, respectively. In addition, the frequency distribution of egg diameter showed peak mode at gonad maturity levels III (0.03-0.17 mm) and IV (0.93-1.07 mm). Based on these findings, yellow-finned medaka was included in the partial or long spawning categories. The results of this study were expected to provide information on the sustainable management, conservation, and domestication of yellow-finned medaka.
Study In Silico Combination of Eucheumma cottonii and Sargassum sp. Extract as The Inhibitor Pir-A Gene of Vibrio parahaemolyticus source AHPND: Combination of Eucheumma cottonii and Sargassum sp. Extract as The Inhibitor Pir-A Gene of Vibrio parahaemolyticus source AHPND Maulita Abrilia; Yuni Kilawati; Rahmi Nurdiani
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.86656

Abstract

Acute Hepatopancreatic Necrosis Disease (AHPND) is a disease in vaname shrimp (Litopenaeus vannamei) caused by PirA and PirB toxins in Vibrio parahaemolyticus bacteria. The active compounds found in the combination of Eucheuma cottonii and Sargassum sp. extracts have the potential to inhibit the PirA toxin protein produced by V. parahaemolyticus bacteria as an alternative to antibiotics. This study aims to predict the interaction between the PirA receptor protein in V. parahaemolyticus bacteria and the active compounds identified from the combination extract of E. cottonii and Sargassum sp. using molecular docking methods. The test results showed that the extract compounds met all ADME predictions, with the best binding affinity value of -6.8 kcal/mol for piperine. This result is similar to the binding affinity value of the antibiotic alpha-Apo-oxytetracycline. The lowest binding affinity value is 3.7 kcal/mol, which is possessed by the Triethanolamine compound. The interaction produced by the piperine compound with the PirA protein target consists of hydrogen bonds in the form of conventional hydrogen bonds and π-donor hydrogen bonds, similar to the antibiotic used as a comparison. The piperine compound also produces π-π stacked interactions, while antibiotics produce π-sigma interactions. The amino acid residues produced by Piperine are ARG39 and TYR110, while the amino acid residues in antibiotics are THR16 and THR24.
Optimization of PCR Annealing Temperature for Pir-A Gene Detection in Vibrio parahaemolyticus Source AHPND in Litopenaeus vannamei By MultiNA Oktaviani Kumala Sari; Yuni Kilawati; Sri Andayani; Kurrotul Uyun; Aditya Ragil Suharto
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.86961

Abstract

Acute Hepatopancreatic Necrosis Disease (AHPND) is a bacterial disease that causes high mortality rates and severe economic losses in Litopenaeus vannamei aquaculture. This disease is associated with pathogenic Vibrio parahaemolyticus strains carrying the PirA virulence gene. Therefore, early and accurate detection of AHPND is essential for effective disease management. Polymerase Chain Reaction (PCR) is widely used for molecular detection; however, its performance is greatly influenced by annealing temperature optimization. This study aimed to determine the optimal PCR annealing temperature for detecting the PirA gene in V. parahaemolyticus isolated from shrimp infected with AHPND using a microfluidic electrophoresis system (MultiNA). Conventional PCR was performed using annealing temperature gradients of 50°C, 55°C, 57°C, and 60°C. PCR products were analyzed using MultiNA to evaluate DNA band patterns, fragment size, concentration, and molarity. The results showed that the pirA gene was successfully amplified at all temperatures tested, producing DNA fragments measuring 134–136 bp. The highest amplification efficiency, specificity, and consistency were obtained at an annealing temperature of 57°C, as indicated by the most dominant DNA band and the highest concentration and molarity values. Lower annealing temperatures resulted in non-specific amplification, while higher temperatures reduced amplification stability. In conclusion, an annealing temperature of 57°C is optimal for PCR-based detection of the pirA gene in V. parahaemolyticus, and the use of the MultiNA system provides accurate and objective PCR product analysis for AHPND diagnostics.
Evaluation of Vibrio parahaemolyticus Immersion Conditions for Infection Establishment and Conventional PCR Detection in Vaname Shrimp (Litopenaeus vannamei): A Preliminary Study: Evaluation of Vibrio parahaemolyticus Immersion Conditions for Infection Establishment and Conventional PCR Detection in Vaname Shrimp (Litopenaeus vannamei): A Preliminary Study Richen Stella Purba; Yuni Kilawati; Muhammad Fakhri; Kurrotul Uyun; Aditya Ragil Suharto
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.87140

Abstract

The Pacific white shrimp (Litopenaeus vannamei) is an important aquaculture commodity, but it is highly susceptible to Acute Hepatopancreatic Necrosis Disease (AHPND) caused by Vibrio parahaemolyticus. Experimental infection models are needed to support molecular detection; however, information regarding the optimal incubation period for successful infection remains limited. This study aims to evaluate the effect of immersion duration and bacterial concentration of V. parahaemolyticus on infection establishment and conventional PCR detectability in a preliminary laboratory-scale challenge test. A laboratory experimental design was employed using seven infection treatments and one negative control, varying bacterial concentration (10⁶–10⁹ CFU/mL), immersion duration, and post-immersion conditions. Shrimp were infected using the immersion method, followed by hepatopancreas sampling for DNA extraction and PirA gene detection via PCR. PCR results were evaluated qualitatively based on the presence of specific amplification bands and PirA concentration. Results showed that shrimp immersed at a concentration of 10⁶ CFU/mL for 24 hours (A1) may represent a promising condition for achieving detectable conventional PCR amplification of the pirA gene while maintaining high shrimp survival. These results indicate that both immersion duration and bacterial concentration may influence the balance between detectable infection and host viability, providing preliminary insight for the development of a sublethal infection model of V. parahaemolyticus in L. vannamei under laboratory-scale conditions. This condition may serve as a practical reference for future experimental infection studies and for the evaluation of preventive or therapeutic strategies against AHPND in shrimp aquaculture.
The The Effect of Different Depths of Growth Rate of Gracilaria sp. in Silvofishery Ponds at in Tambakbulusan Village, Demak: Effect of Different Depths of Growth Rate of Gracilaria sp. in Silvofishery Ponds at in Tambakbulusan Village, Demak Nurul Aulia Dinastuti; Sri Rejeki; Pujiono Wahyu Purnomo
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.87432

Abstract

Seaweed cultivation in the silvofishery system is one of the efforts to utilize environmentally friendly pond land while supporting the sustainability of fisheries production and mangrove ecosystems. The Demak region, especially in the Tambakbulusan village pond area, has great potential for the development of seaweed cultivation of the Gracilaria sp. This study aims to analyze the effect of differences in depth on the growth rate of Gracilaria sp. in silvofishery ponds so as to provide recommendations for farmers in dealing with changes in the aquatic environment. The parameters measured include absolute weight, Specific Growth Rate (SGR), and water quality. The results of the measurement of the absolute weight of Gracilaria sp. showed a difference between cultivation depths of 20 cm (190.67 ± 12.90 grams), 40 cm (155.67 ± 31.21 grams), 60 cm (88.67 ± 14.36 grams). The results of the study on SGR measurements (% / day) of Gracilaria sp. showed differences between cultivation depths of 20 cm (2.37 ± 0.10 grams), 40 cm (2.07 ± 0.47 grams) and 60 cm (1.41 ± 0.12 grams). Water quality data showed temperature (29.4–32.9°C), pH (8–8.1) and dissolved oxygen (3.5–7.4 mg/l). Brightness (33–36 cm), salinity (32.9–33.5 ppt) as well as nitrate (0.67–1 mg/l) and phosphate (0.38–1 mg/l) were sufficient for the growth of Gracilaria sp. seaweed.
Antibacterial Effectiveness of Yellow Turmeric (Curcuma longa) and White Turmeric (Curcuma zedoaria) Extracts against Aeromonas salmonicida: Antibacterial Effectiveness of Yellow Turmeric (Curcuma longa) and White Turmeric (Curcuma zedoaria) Extracts against Aeromonas salmonicida Nisa Hakimah; Budi Rianto Wahidi; Tri Ari Setyastuti; Anja Asmarany; Era Insivitawati; Budi Sugianti; Dewi Nurmalita Suseno; Shara Jayanti
Journal of Aquaculture and Fish Health Vol. 15 No. 2 (2026): JAFH Vol. 15 No. 2 June 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i2.87885

Abstract

Aeromonas salmonicida is a major fish pathogenic bacterium responsible for significant losses in aquaculture. The growing concern over antibiotic resistance has driven interest in plant-based antibacterial alternatives. This study examined the extraction yield, phytochemical characteristics, and in vitro antibacterial activity of ethanolic extracts obtained from the rhizomes of Curcuma longa and Curcuma zedoaria against Aeromonas salmonicida. Extraction was conducted using an ethanol-based maceration technique. Qualitative phytochemical screening was subsequently performed, and antibacterial activity was evaluated through the disc diffusion assay at extract concentrations ranging from 10% to 100%. The results showed that C. longa produced a higher extraction yield (7.18%) than C. zedoaria (3.19%). Both extracts contained flavonoids, total phenolics, alkaloids, and tannins, while saponins were not detected. Antibacterial activity against A. salmonicida increased with extract concentration, reaching maximum inhibition at 100%, with zone diameters of 12.97 ± 0.57 mm (C. longa) and 12.17 ± 0.61 mm (C. zedoaria). Significant differences were observed among concentration treatments. In conclusion, ethanolic extracts of Curcuma longa and Curcuma zedoaria demonstrate promising plant-based antibacterial activity against A. salmonicida in vitro, supporting their potential use in aquaculture health management. Nevertheless, further in vivo studies and toxicity evaluations are required before practical application.

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