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Journal of Aquaculture and Fish Health
Published by Universitas Airlangga
ISSN : 23017309     EISSN : 25280864     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The Journal of Aquaculture And Fish Health (JAFH) has an objective to publish and provide high-quality scientific contributions to the field of fisheries. These contributions came from innovative researches that encourage science and technology development in the field of fisheries and marine science on a national and international scale. This journal serves as a communication medium for researchers, academics, students, and communities.
Arjuna Subject : -
Articles 331 Documents
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Chasmanthera dependens Root Extract as a Dietary Supplement: Impacts on Reproduction Biomarkers in Clarias gariepinus Broodstock Ojetayo, Teslim Asafe; Durojaiye, Abiola Fadilat; Oshoke, Omolegho Justina
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.64696

Abstract

This study investigated the effects of dietary supplementation with Chasmanthera dependens on the reproductive performance and histopathological health of Clarias gariepinus (African Catfish). A total of 75 healthy adult fish were randomly assigned to five dietary treatments: a control group with no additives and four groups receiving C. dependens at 10g, 20g, 30g, and 40g per kg of feed. The experimental duration lasted 90 days, during which parameters such as sperm quality, egg quality, and histopathological observations of gonads were assessed. Results indicated that the control group exhibited superior sperm quality, including the highest milt volume, motility, and live/dead ratio, compared to all treatment groups. Fecundity, egg size, fertilization rates, and hatchability percentages were also highest in the control group. Conversely, groups receiving higher levels of C. dependens demonstrated significantly lower (p<0.05) reproductive performance metrics, suggesting adverse effects linked to excessive supplementation. Histopathological analysis revealed no observable lesions in the testes and ovaries of the control and lower treatment groups, with healthy seminiferous tubules and advanced vitellogenesis. However, the group receiving the highest level of C. dependens exhibited signs of congestion in the ovaries, indicating potential detrimental effects. Overall, while C. dependens may offer reproductive benefits at moderate levels, excessive dietary inclusion negatively impacted sperm and egg quality as well as gonadal health. Further research is recommended to establish optimal inclusion rates for maximizing the beneficial effects of C. dependens while minimizing adverse impacts on reproductive health.
Effect of Feeding Frequency on Feed Utilization Efficiency and Growth of Asian seabass (Lates calcarifer) Fingerlings Dhea Mayang Saputri; Seto Windarto; Diana Chilmawati
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.67912

Abstract

Asian seabass (Lates calcarifer) is an economically important euryhaline fish. Feed absorbs 60–70% of the total production costs; therefore, regulating feeding frequency is necessary to increase feed utilization efficiency. Feeding frequency is the amount of feed given to fish daily. This study aimed to examine the effects of feeding frequency on the utilization of a phytase enzyme in the feed of Asian seabass fingerlings. The experimental fish used were Asian seabass fingerlings (7.57±0.28 cm in length and 5.31±0.15 g in weight) with a stocking density of one fish/L. This research used an experimental method with a completely randomized design (CRD), which consisted of four treatments and three replicates. The treatments were feeding frequencies of 1 (A), 2 (B), 3 (C), and 4 (D) times a day with the addition of 0.1 g/kg feed of the phytase enzyme. The feeding method used was the relative feeding rate, and the feed dose was 6% of the fish biomass. The results showed that feeding frequency significantly affected total feed consumption (TFC), feed conversion ratio (FCR), feed utilization efficiency (FUE), protein efficiency ratio (PER), and relative growth rate (RGR). However, they had no significant effect on the survival rate (SR) of Asian seabass fingerlings. Treatment C (3 times a day) showed the highest TFC, FCR, FUE, PER, and RGR, which were 251.85±1.61 g, 1.45±0.03, 69.08±1.50%, 1.51±0.03, and 3.90±0.11%/day, respectively. The water quality in the study area was suitable for Asian seabass cultivation. This study concluded that feeding three times a day resulted in the best FUE and RGR of Asian seabass fingerlings.
Performance of Growth, Survival Rate, and Behavioral in Seabass (Lates calcarifer) Seed Production with Artemia Enrichment using Sardinella Oil Nisa, Andina Chairun; Ilham, Ilham; Aras, Annisa Khairani; Insani, Liga; Jatayu, Diklawati; Febrianti, Desy; Mahendra, I Gede Rezza
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.68807

Abstract

Essential nutrients for seabass (Lates calcarifer) growth can be derived from the live feed Artemia sp. Artemia sp. It is reported to contain protein levels between 40 and 55%, carbohydrate levels between 15 and 20%, and a lipid content of around 0.4%. Enrichment of Artemia sp. It is necessary to improve its nutritional value and promote seabass larval growth. Sardinella fish oil is one option for enriching Artemia sp. This study aims to determine the optimal dosage of Sardinella oil for Artemia sp. Enrichment to achieve optimal growth and survival rates in seabass (Lates calcarifer) larvae. The study employed a completely randomized design (CRD) with five treatments and three replicates. The treatments were: unenriched Artemia (K-), Artemia + 0.9 g/l Squalene Fish Oil (K+), Artemia + 1 ml Sardinella Oil (A), Artemia + 1 ml Sardinella + 1 ml Egg Yolk (B), and Artemia + 0.5 ml Sardinella Oil + 0.5 ml Egg Yolk (C). The highest absolute weight was observed in treatments A and C, both recording 2.02 g (±0.20 g and ±0.09 g, respectively). Regarding absolute length, treatments A and C also showed the highest values, measuring 1.89 cm (±0.17 g) and 1.90 cm (±0.17 g), respectively. The highest survival rate (SR) was likewise found in treatments A (34.67% ± 2.63%) and C (38.08% ± 3.89%). Based on the tested treatments, the optimal dose for seabass growth and survival (SR) was determined to be treatment C, which included 0.5 ml of Sardinella oil and 0.5 ml of egg yolk.
Effect of Phosphorus Concentration on Sea Grape Growth Rate (Caulerpa racemosa) Amrina Rossada, Rizky; Cokrowati, Nunik; Prasedya, Eka Sunarwidhi
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.70858

Abstract

Sea grapes are one type of seaweed in Indonesia. One of them is Caulerpa racemosa. This study was conducted to determine the effect of phosphorus concentration on the growth rate of C. racemosa with different doses of fertilizer. This study was designed using a completely randomized design (CRD) consisting of four treatments and three replicates. The dose used is 0 ppm (control), 1.5 ppm, 3 ppm, and 4.5 ppm. C. racemosa were reared for 15 days in 35 cm plastic containers with a volume of water used during the rearing period of 10 liters, equipped with aerators and small aeration bubbles set with a stocking density of 25 grams. The results showed that the daily growth rate (DGR) of sea grapes ranged from 5.6-17.55%, final weight ranged from 10-31.3 g, antioxidant activity ranged from 30.49-38.66%, and chlorophyll content ranged from 26.21-31.35 mg/L. The DGR of C. racemosa was not substantially affected (p>0.05) by the fertilizer formulation dose concentrations. Further research needs to be done by adding phosphorus fertilizer doses for standard daily growth rate results.
Physiological and Hematological Responses in Cantang Grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) Against the Salinity of Different Media Larasati, Anastasya Dewi; Widodo, Langgeng; Melinda Kusuma Ningrum; Ridwansyah; Karunia, Fitria; Sulmartiwi, Laksmi; Mahasri, Gunanti; Triastuti, Rr Juni; Lutfiyah, Lailatul
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.71620

Abstract

Fish Cantang grouper is a cross between a female tiger grouper (Ephinepelus fuscoguttatus) and a male kertang grouper (Ephinepelus lanceolatus), has a high economic value. Salinity is a water quality factor that affects cultivation because salinity has osmotic pressure that can cause changes in physiological activity and stress in fish if it is not in accordance with the habitat. Stress in fish causes the release of cortisol and catecholamine hormones. The catecholamine hormone then increases the glycogenolysis process, regulates cardiovascular and respiratory function, in addition to increasing cortisol and blood glucose. Stress in fish is also indicated by a hematological response in the form of changes in the number of erythrocytes. This study aims to determine the effect of different salinity treatments on physiological and hematological responses. The results of the analysis of variance (ANOVA) carried out from the initial observation to 24 hours showed that each salinity treatment had a significantly different effect on blood cortisol, blood glucose, and erythrocyte levels, with a value (p <0.05). The highest average blood cortisol was in the 36 ppt treatment at 24-hour observation time. The average blood glucose increased from before being treated, until receiving treatment for up to 24 hours. The highest average was obtained at a salinity of 36 ppt, with observation for 24 hours. The results obtained from the study showed that the highest number of erythrocyte cells at 24 hours was in the P2 treatment (36 ppt), namely 1.15 x 106 cells/mm³.
Effect of Butterfly Pea (Clitoria ternatea) Flower Fraction on the To-tal Leukocyte Count of Common Carp (Cyprinus carpio) Putri, Rinata Entonnia; Sri Andayani; Seto Sugianto Prabowo Rahardjo
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.72195

Abstract

Intensive common carp (Cyprinus carpio) farming is prone to bacterial infections such as Aeromonas hydrophila, and while antibiotics are effective, their overuse raises concerns about resistance and environmental impact. This study aimed to identify the fractions of butterfly pea flower (Clitoria ternatea) extract through phytochemical tests, UV-Vis, and FTIR spectrophotometry, and evaluate their immunostimulant potential by assessing total leukocyte counts in common carp (C. carpio). The research was conducted from December 2024 to February 2025 at the Fish Disease and Health Laboratory, Brawijaya University. Fifty Majalaya strain common carp were divided into treatment and control groups, receiving 25 ppm extract fractions. Extraction was performed using ethanol, ethyl acetate, and n-hexane, followed by fractionation via thin-layer chromatography (TLC). Leukocyte count analysis was conducted from day 0 to day 3 using microscopy. Results showed that 96% yielded the highest extract recovery (22.16%), while ethyl acetate contained the best bioactive compounds. The optimal TLC eluent was chloroform: methanol (9:1), producing distinct light green and pink spots. Fraction 5 showed the highest leukocyte increase from day 0 (0.73 × 10⁴ cells/mm³) to day 2 (0.29 × 10⁴ cells/mm³) and the lowest decrease on day 3 (0.13 × 10⁴ cells/mm³). UV-Vis spectrophotometry revealed a peak at 659 nm, indicating the presence of phenolics, flavonoids, and alkaloids. FTIR spectroscopy confirmed the presence of key functional groups, including O-H, C-H, C≡N, C=O, and C=C. These findings suggest fractionated butterfly pea flower extract as a promising natural immunostimulant for sustainable aquaculture.
Recent Population of Banggai Cardinalfish (Pterapogon kauderni) in Kendari Bay – Southeast Sulawesi Subhan, Subhan; Prayitno, Slamet Budi; Sabdaningsih, Aninditia; Asriyana, Asriyana
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.72699

Abstract

The Banggai Cardinalfish, Pterapogon kauderni, has been introduced and spread in Kendari Bay, Southeast Sulawesi. The purpose of this study is to estimate the current fish density and distribution of microhabitats in Kendari Bay. Observations of fish populations used the belt transect method at 5 observation stations, with 4 replications per station. Overall fish density ranged from 82±10 individuals/100 m2 to 1,509±583 individuals/100 m2. The fish choose to associated with micorohabitats such as Diadema sp., (69.1%), fire coral from the genus Millepora (16.4%), Sea anemone (10.7%) and seagrass (3.8%). P. kauderni was observed coexisting with other fish species in microhabitats such as around seagrass roots and corals, without showing significant aggressive behavior. Considering its ecological adaptations, P. kauderni is potentially suitable for aquaculture systems that combine multiple species. With the right domestication approach, this species has great potential for development in a sustainable ornamental aquaculture industry.
In Silico Study of Betaine, Isoleucine, and DL-Stachydrine Compounds in Shipworm (Spathoteredo obtusa) Extract as an Antibacterial Agent of Aeromonas hydrophila Andi Al Furqan; Fadjar, Mohamad; Maimunah, Yunita
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.72940

Abstract

The rapid increase in freshwater commodity production is accompanied by various obstacles that pose challenges for farmers, namely, disease infections. Bacterial infection by Aeromonas hydrophila is the pathogenic agent causing Motile Aeromonas Septicemia (MAS), which can result in 100% mortality within a short period. The AhlC toxin protein in A. hydrophila bacteria plays the most critical role in the Ahl tripartite toxin, as AhIC acts as a protomer and inserts itself into one membrane layer, then binds to AhlB and AhlA to form pores in both membrane layers. Active compounds found in marine mussel extracts (Spathoteredo obtusa), particularly betaine, isoleucine, and DL-stachydrine, have the potential to inhibit the AhlC toxin protein produced by A. hydrophila bacteria. This study aims to predict the interaction between the AhlC receptor protein in A. hydrophila bacteria and the active compounds identified from the extract of shipworms (S. obtusa) using molecular docking methods. The test results showed that all three compounds met all ADME predictions, with the best binding affinity value of -4.2 kcal/mol for isoleucine and DL-stachydrine, followed by -3.5 kcal/mol for betaine. Based on the test results, there are appropriate, stable, and effective hydrogen and electrostatic charge interactions with the ligand-receptor complex (ASN:32, GLN:35, ARG:112, and ASP:116), which play a crucial role as active sites in ligand binding to the receptor.
The Sustainability Status of Vannamei Shrimp (Litopenaeus vannamei) Aquaculture in Millennial Pond Model: Sustainability Status of Vannamei Shrimp (Litopenaeus vannamei) Aquaculture in Millennial Pond Model Arif, Gunarso; Prayitno, Slamet Budi; Purnomo, Pujiono Wahyu
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.73619

Abstract

Vannamei shrimp (Litopenaeus vannamei) aquaculture activities in Jepara Regency are conducted at Brackish Water Aquaculture Centers (Balai Besar Perikanan Budidaya Air Payau/BBPBAP) Jepara with a millennial model. This aquaculture model features a critical point in the middle of the pond, which aims to reduce the risk of failure due to pathogenic virus attacks. However, in running a vannamei shrimp aquaculture, an appropriate strategy is required for business development and sustainability. In this study, a multidimensional scaling (MDS) method was used to analyze the ecological, economic, social, technological, and legal-institutional dimensions. The study was conducted during October-December 2024, in which primary data collection was carried out through interviews with respondents and secondary data from related data references. The results of the study showed that the sustainability values were 88.39 (ecological dimension), 81.05 (economic dimension), 75.25 (social dimension), 99.996 (technological dimension), and 99.996 (legal-institutional dimension). The average value of the sustainability index of vannamei shrimp farming in the millennial pond of BBPBAP Jepara was very sustainable, with a score of 88.94. Based on the leverage analysis, sensitive attributes obtained in this study were ecological domain (1) disease attacks (2) land utilization level (3) land suitability, economic domain (1) price trends, (2) market reach, (3) cultivation profits, social domain (1) education level, (2) community involvement/labor absorption, (3) availability of transportation and communication facilities, technology domain (1) pond construction (2) application of biosecurity and legal domain & (1) institutional training activities and socialization about cultivation businesses (2) availability of cultivator groups.
Hematological Profile of Tilapia (Oreochromis niloticus) as a Bioin-dicator to Evaluate Water Quality of Floating Net Cage Cultivation System in Ranu Grati, Indonesia Alfarisi, Muhammad Asnin; Hertika, Asus Maizar Suryanto; Yanuhar, Uun
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.75605

Abstract

Ranu Grati is a volcanic lake situated in Grati District, Pasuruan Regency, East Java, Indonesia. The rapid expansion of floating net cage aquaculture in Ranu Grati has raised concerns about water quality deterioration and its impact on fish health. This study aimed to evaluate the hematological profile of tilapia (Oreochromis niloticus) as a bioindicator for assessing water quality in floating net cage cultivation systems. The purpose of this research was to examine the hematological profile of tilapia fish alongside a series of water quality parameter measures as an environmental factor influencing fish life. Sampling was conducted with three repetitions every three weeks from September to November 2024 at five distinct locations, which were deemed representative of each group of floating net cages distributed around the Ranu Grati region. The Canonical Correspondence Analysis (CCA) approach was utilized to ascertain the relationship between hematological profile of tilapia and water quality. The data analysis indicates that the water quality at Stations 2 and 3 in the research area was somewhat polluted, but Stations 1 and 5 were heavily polluted. The elevated pollution levels at Stations 1 and 5 are attributed to the significant quantity of floating net cages in the vicinity relative to other stations. The investigation indicated that low dissolved oxygen levels and elevated ammonia concentrations impacted the hematological parameters of Tilapia fish. All water quality characteristics influenced the status of leukocytes, erythrocytes, and hemoglobin at moderate quantities. Moreover, micronuclei and hematocrit levels were significantly affected by elevated concentrations of ammonia, temperature, BOD, and pH, whereas DO, TDS, and TSS influenced lower values.

Page 33 of 34 | Total Record : 331

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