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<![CDATA[Pengaruh Media (Campuran Beras Dan Ampas Tebu) terhadap Pertumbuhan Trichoderma harzianum dan Daya Hambatnya terhadap Fusarium oxysporum secara In vitro ]]>
<![CDATA[Moralita Chatri]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02018219984-0-00
<![CDATA[Jamur Fusarium oxysporum merupakan jamur patogen umumnya ditemukan pada tanaman holtikultura. Jamur ini menyebabkan penyakit layu fusarium. Fungisida sintetis merupakan pilihan yang sering digunakan petani untuk menghambat penyebaran jamur ini. Namun penggunaannya menimbulkan banyak dampak negatif bagi lingkungan. Trichoderma harzianum memiliki sifat antagonis yang dapat menggantikan fungisida sintetis dalam menghambat pertumbuhan F. oxysporum. T. harzianum membutuhkan media pembawa seperti beras. Namun membutuhkan biaya yang cukup tinggi dan bersaing dengan kebutuhan manusia. Untuk itu dilakukan campuran antara beras dan ampas tebu untuk mencari media alternatif. Tujuannya agar mendapatkan media tumbuh yang lebih efektif dan efisien.Penelitian ini menggunakan Rancangan Acak Lengkap dengan 5 perlakuan dan 3 ulangan. Perlakuanya terdiri dari A. Media beras 100%, B. Media ampas tebu 100%, C. Media beras dan ampas tebu (1:1), D. Media beras dan ampas tebu (2:1), dan E. Media beras dan ampas tebu (1:2). Paremeter yang diamati yaitu pertumbuhan T. harzianum, kepadatan konidia, dan persentase hambatan terhadap F. oxysporum.Hasil penelitian ini menunjukkan bahwa T. harzianum dapat tumbuh pada campuran media beras dengan ampas tebu. Media tumbuh mempengaruhi pertumbuhan, kepadatan konidia, serta daya hambatnya terhadap F. oxysporum Pertumbuhan yang terbaik yaitu pada media ampas tebu 100 %, kepadatan konidia tertinggi pada media beras 100% (11,6 x 109/ mL) dan persentase daya hambat tertinggi pada media ampas tebu 100% (60%). Hal ini menunjukkan bahwa media alternatif terbaik yaitu media ampas tebu 100%. Keywork : Fusarium oxysporum, layu fusarium, media, Trichoderma harzianum ]]>
<![CDATA[Ecological Preference of Soil Texture to Distribution of Mangrove Seedling Species in the Forest Mangrove Teluk Buo, Padang-Sumatera Barat ]]>
<![CDATA[irma - leilani]]>;
<![CDATA[Hendra Lardiman]]>;
<![CDATA[Mades Fifendy]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/020182110042-0-00
<![CDATA[A research has been conducted to find out the ecological preferences of soil texture on seedling of mangrove species in mangrove forest of Teluk Buo, Padang of West Sumatra. The study was done from May to July 2008. Samples were taken by making 3 transect lines perpendicular to the coastline. 26 observation plot 10 x 10 m was placed continuously zigz zag left to right along the transect line. In each plot is recorded the species of seedling were found and soil texture. Soil texture analized in the lab. Biology FMIPA-UNP. The study revealed that there is an ecological freferences the soil texture to the distribution of seeding of mangrove species. The seedling of Rhizophora apiculata, Ceriop tagal and Sonneratia alba was collected in areas with dusty clay soil texture. The seedling of Avicennia marina was collected on sandy loam soil and sand. The seedling of Bruguiera gymnorhyza only present on the clay sandy soil.]]>
<![CDATA[PERBANDINGAN NILAI RERATA LED METODE WESTERGREEN MENGGUNAKAN DARAH EDTA DAN NaCl 0,86 % DENGAN PERBANDINGAN PENGENCERAN 4 : 0,5 DAN 4 : 1 PADA PASIEN TERSUSPEK TB PARU ]]>
<![CDATA[Farah Diba Setiana]]>;
<![CDATA[Heru Purwita]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02018219968-0-00
<![CDATA[Pemeriksaan Laju Endap Darah adalah pemeriksaan yang dilakukan untuk menentukan kecepatan pengendapan eritrosit dalam suatu tabung yang diletakkan dalam posisi tegak lurus yang dinyatakan dalam satuan mm/jam. Tujuan pemeriksaan ini untuk mengetahui perbandingan nilai rerata Laju Endap Darah (LED) metode Westergren dengan darah EDTA yang di tambah NaCl 0,86% perbandingan 4 : 0,5 dan 4 : 1 pada pasien tersuspec TB paru. Metode yang digunakan adalah metode Westergren. Dalam penelitian ini menggunakan 2 macam perbandingan pengenceran darah EDTA dan NaCl 0,86% yaitu perbandingan 4 : 0,5 dan 4 : 1 Penelitian ini telah dilakukan pada bulan November 2017 di Laboratorium klinik EMC Kota Tangerang, terhadap 32 pasien tersuspek TB paru yang melakukan pemeriksaan LED. Uji statistik yang digunakan adalah pengujian Mann Whitney. Hasil pada penelitian ini secara statistik adalah, perbandingan nilai rerata LED metode Westergreen dengan perbandingan pengenceran 4 : 0,5 dan 4 : 1 pada pasien teruspek TB paru menunjukkan hasil yang berbeda bermakna dengan nilai kemaknaan p=0,004. Nilai LED pada perbandingan pengenceran 4 : 0,5 diperoleh hasil yang lebih tinggi yaitu 39,1 mm/jam dibanding nilai LED pada pengenceran 4 : 1 yaitu 25,8 mm/jam, sedangkan nilai LED normal sebesar 20 mm/jam. Tingginya nilai LED ini disebabkan karena sampel darah yang digunakan adalah sampel darah dari pasien tersuspek TB paru.]]>
<![CDATA[GHS: Keselamatan Berbicara Melalui Simbol ]]>
<![CDATA[Arif Sardi]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02018219843-0-00
<![CDATA[Tanda dan simbol pencegahan bahaya adalah alat komunikasi keselamatan yang penting, mereka membantu menunjukkan berbagai bahaya yang ada di laboratorium. Pada saat yang sama, simbol pencegahan bahaya memperingatkan praktikan agar selalu waspada terhadap bahaya tersebut dengan memberikan informasi dan instruksi keselamatan yang dibutuhkan. Simbol pencegahan bahaya bisa mengkomunikasikan pesan dengan cepat dan mengatasi hambatan bahasa. Pada tahun 2015, perubahan besar terjadi pada label bahan kimia dan SDS secara global. Ada bahasa baru dari simbol pencegahan bahaya yang harus dipahami oleh para pengguna bahan kimia. Beberapa produsen bahan kimia telah mengeluarkan jenis simbol baru untuk pencegahan bahaya. Hal ini sebagai implikasi dari telah diadopsinya regulasi GHS (Globally Harmonized System) di beberapa negara Uni Eropa dan Amerika Serikat. Banyak orang yang bekerja dan mengajar dengan bahan kimia masih belum mengetahui secara rinci tentang apa yang telah berubah dan apa artinya bagi mereka.]]>
<![CDATA[TOTAL MICROBIAL PLATES ON BEEF AND BEEF OFFAL ]]>
<![CDATA[Sukmawati Sukmawati]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02018219825-0-00
<![CDATA[Efforts to improve people’s nutritional health can be obtained through nutritional food. Beef is a source of animal protein needed. But foodstuffs such as meat and eggs other than as a source of protein is also one of the microorganisms breeding media, which can be a type of human disease. The aim this study was to find out whether the sample of beef, beef heart, and beef liver are safe to consume and marketable. This research method includes tool sterilization, microbial growth medium, and the analysis phase used total plate count method. Samples of beef number of microbial colonies as much as 660 cells/ml. samples of cow’s heart contained colonies 3.150 cells/ml, and in liver samples there are 3.000 cells/ml. so it can be concluded that beef and dairy products are feasible to marketable and to consumption. Keywords: Total Microbial, Beef, Beef Offal]]>
<![CDATA[Correlation of Fasting Blood Glucose With IL-6 Levels in Type-2 Diabetes Mellitus Ethnic Minangkabau ]]>
<![CDATA[Elsa Yuniarti]]>;
<![CDATA[Syam Syamsurizal]]>;
<![CDATA[Yuni Ahda]]>;
<![CDATA[Puja Delfi Sonata]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02018219858-0-00
<![CDATA[Diabetes Mellitus (DM) Type 2 is a metabolic disorder disease characterized by an increase in blood sugar (hyperglycemia) due to decreased insulin secretion by pancreatic beta cells or intrusion of insulin function. Hyperglycemia tends to cause oxidative stress where free radical formation exceeds the body's antioxidant defense system resulting in microvascular and macrovascular disorders. Some ethnic groups have a tendency to develop type 2 diabetes mellitus because of differences in diet, lifestyle and physical activity. In addition, ethnicity is also suspected to affect the levels of IL-6. This study aims to determine the correlation of fasting blood sugar levels with IL-6 levels in type 2 diabetes mellitus ethnic Minangkabau. This research is cross sectional comparative research design. The subjects of two groups: DM type 2 and control group (non-DM) who went to the Polyclinic of State University of Padang, Minangkabau ethnic and each consisted of 35 people. Blood glucometer examination with blood sample and IL-6 measurement using ELISA technique with serum sample. Fasting blood sugar levels in patients with Type 2 DM Minangkabau ethnic average 286.2 ± 80.46 mg / dl while non DM 101.26 ± 9.70 mg / dl. Mean IL-6 levels in type 2 DM patients were 16.23 ± 30.12 pg / ml while non-DM of 3.41 ± 1.91 pg / ml was an increase of about 4.7 in patients with type 2 DM Compared to non-ethnic minority of Minangkabau with p value <0,05. The result of research that have been done there is a positive correlation between fasting blood sugar level with IL -6 in patients with Type 2 DM Minangkabau ethnic with r value = 0,44 at p <0.05 which means interpretation of medium correlation strength. Keywords: Type 2 DM, IL-6, ethnic Minangkabau]]>
<![CDATA[Sudut ATD sebagai Penanda Diabetes Mellitus Tipe-2 (DMT2) ]]>
<![CDATA[Syam Syamsurizal]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/020182110128-0-00
<![CDATA[Dermatoglyphic of patterns (finger prints) could be used as genetic markers with type 2 diabetes. Analysts dermatoglyphic patterns can quantitatively through several parameters: frequency fingerprint patterns (arch, loop and whorl), the number of vines, pattern type index consists of the Dankmeijer index, Furuhata index, pattern intensity index and atd angle. Atd angle is the angle formed by connecting point triradius a, t and d on the palm area. Objective studies link dermatoglyphic characteristics (atd angle) with type 2 diabetes mellitus in Minangkabau ethnic.The research method used descriptive with 132 sample consisted of 66 patients with type-2 diabetes mellitus and 66 control. Results of research on atd angle in type 2 diabetes was 40,44± 3,34 and 40,42±3,96 in controls. The results of t-test and α = 5% p value = 0.75. It can be concluded that there was no significant difference in the average of atd angle in diabetes mellitus type 2 Minangkabau ethnic.]]>
<![CDATA[Isolation of Phosphate Solubilizing Endophytic Fungi From Rice Plant Root ]]>
<![CDATA[Dezi Handayani]]>;
<![CDATA[Mades Fifendy]]>;
<![CDATA[Verawati Yesni]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/020182110043-0-00
<![CDATA[Root endophytic fungi plays different roles for plant, such as plant growth promoting properties, agents to control phytopathogens, and increase phosphorus uptake. Since phosphorus are essential for plant growth and its occurance are limited, so it is necessary to explore these fungus to replace the used of synthetic fertilizer. The objective of this study were to obtain root endophytic fungi from rice plant and to determine its phosphate solubilization ability. The root organ of rice plant was subjected for isolation. Pikovskaya medium was use to determine the fungal phosphorus solubilization ability. Fungal morphological characteristics was carried out by macroscopic and microscopic appearance assessment using microscope. Seven endophytic fungi were successfully isolated from rice plant root sample. Four isolate were micelial steril with no conidia, two isolate refers to Aspergillus and 1 isolate have 2-4 conidia at the tip of conidiophores. Amongs 7 endophytics fungi, only one isolate (P2B3) had the ability to solubilize phosphate with the phosphate solubilization index value 20.45 %.]]>
<![CDATA[Coliform Contaminant Analysis at Dug Well in Ungga Village, Central Lombok District, West Nusa Tenggara ]]>
<![CDATA[Dadan Supardan]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/02018219981-0-00
<![CDATA[Dug well water generally is a daily source of domestic water used by most households in Indonesia. However, the well water excavation does not meet the quality of health standards. Contamination of microorganism in dug well water occurs due to the distance of wells with septic tank, household waste disposal and animal husbandry. One of the bioindicators of contaminated groundwater is the presence of Coliform bacteria in large quantities, such as Escherichia coli, which potentially cause various diseases in human. The objective of this study was to determine the biological contamination of dug wells water in Ungga, Iting Bengkel Village, Central Lombok. This study was observational study and sampling method used purposive sampling with the number of samples was 6 dug wells. The biological contamination indicator was determined by calculating the index of Most Probable Number (MPN) Coliform. The laboratory analysis included the analysis Escherichia coli by MPN. The result showed that a total of 6 wells with distance 3.45 meters and 4.70 meters against septic tank has MPN coliform value 1898 per 100 ml. The result of water samples from 2 dug wells has contaminated by Escherichia Coli and has reached amount 14 per 100 ml (A3) and 16 per 100 ml (B3). ]]>
<![CDATA[Optimasi Sistem Transformasi Gen Xiloglukanase Pada Eucalyptus pellita F. Muell Menggunakan Agrobacterium tumefaciens ]]>
<![CDATA[Sri Hartati]]>;
<![CDATA[Andriyani Puspitaningrum]]>;
<![CDATA[Nita Etikawati]]>;
<![CDATA[Enny Sudarmonowati]]>
<![CDATA[ Bioscience Vol 2, No 1 (2018): Biology ]]>
Publisher : <![CDATA[UNIVERSITAS NEGERI PADANG ]]>
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DOI: 10.24036/020182110044-0-00
<![CDATA[Eucalyptus pellita is a type of woody plant that is widely used as raw materials of pulp and paper so that the need for wood from this type of plant is increasing. Improvements in wood quality such as cellulose deposition and increased growth rates are needed to support the supply of raw materials for the pulp and paper industry. One technology to change the composition of wood is the modification of plant cell walls through the transformation of xylolukanase gene which in other plants such as Populus alba and Acacia mangium have been shown to increase cellulose deposition and spur growth. The purpose of this study was to obtain an efficient xyloglucanase transformation method in E. pellita using Agrobacterium tumefaciens. Sprouts E. pellita 006 and 06A with different ages of 8 and 15 days are used as plant material for transformation. The sonication treatment of the sprouts prior to the transformation was also applied to determine the effect on transformation efficiency. Transformation is done by soaking the seeds that have been through the treatment of sonication and without sonication on the suspension of Agrobacterium carrying plasmid pAa XEG300 and subsequently grown on the selection medium. Sprouts E. pellita 006 aged 15 days without sonication treatment showed the highest percentage of regeneration in the selection media that is equal to 72%. Gene integration testing through DNA amplification with specific primers showed a ribbon of xyloglucanase with a size of 709 bp.]]>
