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Debora Christin Purbani
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Journal of Microbial Systematics and Biotechnology
Published by Cibinong Science Center
ISSN : -     EISSN : 26854430     DOI : https://doi.org/10.37604/jmsb
Core Subject : Science,
Immunology & microbiology
Journal of Microbial Systematics and Biotechnology (JMSB) is the international journal of microbial diversity and microbial technology which publishes research articles, reviews, and methodologies of microbial-based technology; and taxonomic articles such as monographs, new species, new notes, new records, checklists related to microbial diversity. The official language is English. Every manuscript submitted to JMSB will be published as soon as the editor receives it, and through the peer review process.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi
Articles 32 Documents
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Cover JMSB Vol 2, No 2 (2020) Cover and Table of Content JMSB
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.65

Abstract

Microbial isolation from gastrointestinal tract of catfish (Clarias gariepinus) Nurul Aini; Muhammad Bachruddin; Izdihar Tsana
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.38

Abstract

The purpose of this research was to describe the characteristics and number of microbes that grow in the gastrointestinal tract of catfish and to determine the potential of microbes as probiotics. The type of this research was observational conducted on 30 September-15October 2018 at the Microbiology Laboratory, Faculty of Science and Technology, Universitas Airlangga, Surabaya. Microbial isolation using Nutrient Agar (NA), Mann de Roosa Shrape agar (MRSA) and potato dextrose agar (PDA) media. The fish that used is catfish that are bred in ponds at Desa Tlasih, Kecamatan Tulangan, Kabupaten Sidoarjo, East Java. The identification includes microscopic and macroscopic characteristics. Based on the research that has been done, it can be concluded that there are 8 kinds of bacterial colonies in NA media, on PDA media there are 8 kinds of fungi, and on MRSA media there are 2 bacterial colonies and each has different macroscopic and microscopic characteristics. The total number of bacteria growing in NA and MRSA media respectively were 8.7 x 104 CFU/g and 1.2 x 105 CFU/g. It is suspected that there are potential bacteria as beneficial probiotics for catfish which still need further research.
Culturable bacterial abundance in Volvariella volvacea cultivation medium and characterization of its bacteria Masrukhin Masrukhin; Iwan Saskiawan
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.52

Abstract

Straw mushroom (Volvariella volvacea) is one of the popular edible fungi in Indonesia. Previous researches showed the correlation among the type of substrate, substrate quality, and its composting process to the microbial community, yield, and biological efficiency. The aim of the research is to analyze the culturable bacteria abundance in straw mushroom cultivation medium, characterize the bacteria in several stages of mushroom cultivation and investigate the interaction between V. volvacea with its resident bacteria. Samples were taken from mushroom farmers in Subang and Karawang regencies, Indonesia. The materials for cultivation medium are the mixture of cotton and paddy straw and the pasteurization was performed at 65-70°C for 7 hours. The result shows the abundance of the bacteria in most of the cultivation stages is relatively similar i.e. 108 CFU/g, except in 15 days after inoculation (DAI), the bacterial abundance is lower i.e.6.24 x 107 CFU/g. Twenty-five isolates were obtained and Gram-positive bacteria is the dominant bacteria found in the cultivation medium, especially rod-shaped Gram-positive bacteria. According to co-culture assay there are nine isolates that decrease the growth rate and clearly inhibit mycelial growth. The other 10 isolates have lower inhibitory activity, and 6 isolates have no inhibitory activity to the mycelial growth. C38 isolates have the highest mycelial growth inhibition. It belongs to rod-shaped Gram positive group of bacteria which isolated from the early stage of V. volvacea cultivation medium (5 DAI).
Challenge of bacteriophage application to improve food safety and its administration into the human gut: an article review Qori Emilia
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.36

Abstract

Ensuring microbial food safety has always been a challenge at every stages along the food chain. Meanwhile, healthier community lifestyle demands natural antimicrobial agents to alleviate the increasing use of chemical preservatives to address microbial contamination. Antimicrobial resistance issue has also elevated the effort to search for an alternative way to antibiotics. Bacteriophage (phage) is currently being assessed for its potency as a biocontrol agent to enhance food safety and as a tool for therapeutic purposes. Prior to phage application, safety assessment must be conducted in which includes several considerations: from the discovery, toxicological aspects to the impact of phage ingestion on the gut microbiota. The gut microbiota which consist of variety of microorganisms inside the human gastrointestinal tract, cohabitate to each other. Phage is naturally present as one of microorganisms in the human gut and dynamically interacted with other microbial communities. Phage application to foods and food-contact surfaces may leave a residue and cause the phages to be ingested, which in result may alter the gut microbiota composition. Many findings have examined the relationship between gut microbiota and human health, and so is the factors affecting their modulation. This review aimed to discuss several points of view from published research papers related to the challenge of phage to improve food safety and its administration into the human gut.
Effect of ethanol extract from Karuk leaf (Piper sarmentosum Roxb.) on the growth of Malassezia furfur in vitro Khusnul Khusnul; Asti Kusmayanti; Lia Aulia Rahman; Nuniek I Ratnaningtyas
Journal of Microbial Systematics and Biotechnology Vol 2, No 2 (2020): December 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i2.59

Abstract

In Indonesia, there are numerous therapeutic plants found. Some of the plants used in herbal medicine are Karuk leaf (Piper sarmentosum Roxb.) belong to the Piperaceae family. Karuk leaf has chemical contents such as saponins, polyphenols, flavonoids, and essential oils and many tests are carried out on several bacteria, but testing of fungi is rarely studied. This study aims to determine the ethanol extract activities from karuk leaf in inhibiting the growth of the Malassezia furfur fungus and to determine its minimum inhibition by using the Kirby-Bauer method. The study was conducted by an experimental method of the M. furfur fungus using the Kirby-Bauer method. The ethanol extract from karuk leaf was made in various concentrations and tested on 0.5 McFarland fungus by diffusion on Sabouraud Dextrose Agar (SDA). The results of this analysis showed that the ethanol extract of Karuk leaf could inhibit the M. furfur fungus at a concentration of 30% by 5.3 mm, 40% by 6.6 mm, 50% by 7.6 mm, 60% by 11.3 mm, 70% by 12.5 mm, 80% by 15.6 mm, 90% by 17.4 mm, and 100% by 19.5 mm. Based on the results of the study and the data analysis, it can be concluded that several concentrations of ethanol extract of Karuk leaf affect the growth of M. furfur in vitro.
A molecular phylogenetic study of Clarohilum henningsii (Mycosphaerellaceae, Fungi) on cassava from Indonesia based on the ITS rDNA sequence Iman Hidayat; Aerma Hastuty; Indriati Ramadhani
Journal of Microbial Systematics and Biotechnology Vol 2, No 1 (2020): June 2020
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v2i1.43

Abstract

Leaf spot is a common disease of cassava in Indonesia and other tropical countries. The present study aims to determine two isolates isolated from leaf spot of cassava in Indonesia using a molecular phylogenetic analysis based on internal transcribed spacer (ITS) rDNA sequence and morphological examination. The results showed that the two isolates belong to Clarohilum henningsii. This study revealed the phylogenetic position of C. henningsii from Indonesia, where in the previous studies, it has been reported as Cercospora cassavae, C. manihotis, C. henningsii, or Passalora henningsii based on morphological examinations.
Biosynthesis and profiling of single cell carotenoids of Phaf ia rhodozyma in waste-based cultivation media Arif Nurkanto; Rini Handayani; Ismu Purnaningsih; Mia Kusmiati; Ninu Setianingrum; Mulyadi Mulyadi; Endang Kusdiyanti; Achmad Dinoto
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.61

Abstract

In order to investigate the feasibility of low-cost media for producing well- characterized single-cell carotenoid, the study on biosynthesis and profiling carotenoid in the yeast of Phaf ia rhodozyma was carried out. We have successfully identified the profiles of single-cell carotenoids of P. rhodozyma, which was cultivated in glucose-based medium (MG), molasses-based medium (MM), and coconut water-based medium (MC). Cells were separately cultured in those media under aerobic batch culture system to obtain the carotenoid profiles based on high performance liquid chromatography (HPLC) analysis. The results showed that medium composition strongly affects the profiles of P. rhodozyma carotenoids represented by ratio of astaxanthin and beta-carotene (ratio A/B). Astaxanthin was highly synthesized in cells cultivated in MG with ratio A/B as much of 20:1. On the other hand, MM and MC produced a lower ratio A/B than MG as much of 0.4:1 and 0.2:1, respectively. In addition, carotenoids profiles were detected more diverse when this yeast species was cultivated in two waste-based media. This study provided a basic physiological knowledge of P. rhodozyma cells for carotenoid biosynthesis using potential low cost cultivation m
Cover and Table of Content JMSB Vol 3, No 1 (2021) Cover and Table of Content JMSB
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.82

Abstract

Analysis of the SARS-CoV-2 envelope (E), nucleocapsid (N), and non-structural protein12 (nsp12) genes from COVID-19 patients in West Java Azzania Fibriani; Irin Annisa Evitayani; Gusti Ayu Prani Pradani; Rebecca Stephanie; Ema Rahmawati; Ryan Bayusantika Ristandi; Cut Nur Cinthia Alamanda; Rifky Waluyajati Rachman; Rini Robiani; Isak Solihin
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.66

Abstract

According to World Health Organization, as of January 2021, Indonesia is the only Southeast Asian country in which COVID-19 is still occurring in community transmission. West Java is one of the provinces holding the highest positive cases number. With the envelope (E), nucleocapsid (N), and non-structural protein 12 (nsp12) being the target genes of SARS-CoV-2 diagnostic kits and several antiviral drugs, the study of genetic variations has become relevant and greatly important. Out of 267 oro-nasopharyngeal swab specimens that were previously confirmed positive for COVID-19 in qPCR diagnostic test in Laboratorium Kesehatan Provinsi Jawa Barat, ten samples with acceptable qualities were selected and three samples were sequenced using Sanger sequencing. Nonsynonymous mutations were observed in the envelope gene (L21F) and in the nucleocapsid genes (R203K, G204R, A211S, and S193I). Phylogenetic analysis showed that samples were clustered with other sequences carrying identical mutations, but clustered non-discriminatively with all sequences when carrying no mutation. No pattern in geographical areas and clades, except for R203K-G204R for being a marker for the GR clade. Protein structure analysis showed that mutations observed did not change the hydrophobicity and the secondary structure of the nucleocapsid, while stability change (ΔΔG) showed that all mutations, aside from the R203K-G204R, have neutral effect on the protein stability. Therefore, it can be concluded that mutations observed in this experiment did not impart preference to disperse in certain geographical areas or cause any significant structural change in the protein.
Mycorrhiza stimulates Rhizobium infection in Paraserianthes falcataria (L.) I.C. Nielson under Hg contamination Irma Latifah; Idris Idris; Toga Pangihotan Napitupulu; Azra Zahra Nadirah Ikhwani; Gunawan Ruhiyat; I Nyoman Sumerta; Atit Kanti; Fitri Yola Amandita; I Made Sudiana
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.70

Abstract

Symbiosis of and AMF increase soybean production, but the information on the association between these microbes in Paraserianthes falcataria (L.) I.C. Nielson or ‘sengon’ under Hg contamination is limited. We observed P. falcataria inoculated with arbuscular mycorrhiza fungi (Glomus sp.) stimulate nodule formation under Hg contamination. The study was set up in a pot experiment in the growth chamber a the Hg concentration was adjusted to 50 ppm in soil medium. Inoculation of AMF stimulates nodule formation and increases P. falcataria tolerance to Hg. We isolated several bacteria from the rhizosphere belonging to Rhizobium group and others. Understanding the ecology of soil bacteria is important for Hg bioremediation using P. falcataria.

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