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Journal of Microbial Systematics and Biotechnology

jmsb
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Published by Cibinong Science Center

ISSN : - EISSN : 26854430 DOI : https://doi.org/10.37604/jmsb

Core Subject : Science,

Immunology & microbiology

Journal of Microbial Systematics and Biotechnology (JMSB) is the international journal of microbial diversity and microbial technology which publishes research articles, reviews, and methodologies of microbial-based technology; and taxonomic articles such as monographs, new species, new notes, new records, checklists related to microbial diversity. The official language is English. Every manuscript submitted to JMSB will be published as soon as the editor receives it, and through the peer review process.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi

Articles 32 Documents

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Determining the potential indigenous red-yeasts producing β-carotene and their phylogenetic relationship I Nyoman Sumerta; Yeni Yuliani; Atit Kanti
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Red-yeasts are pigmented yeast species that could synthesize carotenoids for food supplements and pharmaceutical purposes. However, this group contains a lot of species which need to be explored thoroughly. The objective of this study was to view the β-carotene production of three indigenous red-yeasts by modifying glucose content in the growth medium and verifying their phylogenetic relationship. The glucose content modification in growth media influenced the β-carotene production of each species. Rhodosporidiobolus ruineniae (InaCCY1638/Y15Eg075) and Rhodosporidiobolus poonsokiae (InaCCY1606/Y15Kr068) produced higher β-carotene than Rhodotorula paludigena (InaCCY1527/Y15Eg004). These Rhodosporidiobolus species were able to produce higher β-carotene from 0.5 to 2% glucose content while Rhodotorula was low production in 2% glucose content. The higher producers by Rhodosporidiobolus species were clustered to Ruineniae clade and could be a potential clade for higher β-carotene production. Based on this result, using a high carotenoid producer of red-yeasts from indigenous strains is potential to be developed for β-carotene bioindustry in the future.

PCR-based Specific Detection of Bacillus in Liquid Organic Fertilizer Iman Hidayat; Nur Laili; Dwi Agustiyani; Sarjiya Antonius
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Rapid molecular PCR-based detection method for Bacillus species used in the production of Beyonic® liquid organic fertilizer was carried out based on nucleotide sequence data from the 16S rRNA gene. The method involved sequencing the 16S rRNA gene of several Bacillus species and identifying around 16-22 specific nucleotide bases from 5' and 3' ends in the Bacillus 16S rRNA gene sequences. One specific primer pair for Bacillus detection was determined as follow: 5' - CAT AAG ACT GGG ATA ACT CCG GG - 3' (forward) from positions of 85-107 bp, and 5’ - CCA GGC GGA GTG CTT AAT GC - 3’ (reverse) from positions of 836-854 bp. PCR assay and gel electrophoresis analysis showed that the primer pair was specific to the genus Bacillus.

Cover JMSB Vol 1, No 1 (2019) JMSB JMSB
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Fortification of Mung bean (Vigna radiata) and Ear mushroom (Auricularia auricula-judae) in dried sago noodles Donowati Tjokrokusumo; Fiqi Cahyani Octaviani; Raskita Saragih
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

This study aimed to obtain a formula dried sago noodles enriched with mung beans and ear mushrooms to produce noodles with high protein and fiber content and favored by the panelist. The experimental design used in this study was a Randomized Block Design (RCBD) with two factorial (3×3). Factor A is the percentage of mung bean flour which consists of three levels: a1 = 12%, a2 = 15%, a3 = 18%. Factor B is the percentage of ear mushroom flour consisting of three levels: b1 = 1%, b2 = 2% and b3 = 3%, with 2 replications. The organoleptic testing was done by 30 untrained panelists. The results showed that the most preferred panelists are dried noodles sago enriched with 12% mung bean flour and 1% ear mushroom flour. The resulting product has an overall value of 14 consisting of an average value of color 3.73 (ordinary-like), aroma preference value 3.3 (ordinary-like), texture preference value 3.47 (ordinary-like), and taste preference value 3.50 (ordinary-like).

Anti-oxidant and anti-bacterial activities of 11 Calophyllum species from Indonesia Jamilah Abbas
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Members of Calophyllum (Calophyllaceae) are commonly used as herbal medicine in Indonesia from the beginning of civilization. Due to their tremendous potential as a source for pharmaceutical industry, we selected 11 Calophyllum species for the current anti-oxidant and anti-bacterial studies, namely, Calophyllum euryphyllum, C. bicolor, C. flavoramulum, C incrassaptum, C. lowei, C. macrophyllum, C. nodosum, C. soulattri, C. tetrapterum, C. teysmannii, and C. tomentosum. The methods include Calophyllum stem bark extraction and separation into n-hexane, ethyl acetate, and butanol fractions, followed by the antioxidant analysis using DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging) and the anti-bacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa. The results showed that n-hexane fraction of C. soulattri exhibited the best anti-oxidant activity (IC50 = 2.2 µg/m). This study also showed that a xanthone compound isolated from C. macrophyllum, namely, 1,8 - dihydroxy-6 methoxy xanthone exhibited the anti-oxidant activity with IC50 = 1.56 ppm.

Biosorption chrome (Cr) and dyes using biosorbent in the modified tea bag Sri Lestari; Ratna Stia Dewi; Eko Setio Wibowo; Atang `
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Biosorption has been known as a potential method in reducing heavy metals and dyes in wastewater, including chrome (Cr) and dyes contents of the wastewater from the batik industry. Straw and spent mushroom compost (SMC) are potential biosorbents due to cheap, abundant, and easily obtained. However, the effectivity of the biosorbents is not only depended upon the type of material but also their surface area. In this study, a modified tea bag was used to increase the surface area of the straw and spent mushroom compost to absorb Cr and dyes from the batik industry wastewater. The optimum of biosorbent ratio and pH in Cr absorption and dyes decolorization was measured. This experiment was conducted using Spilt Plot Design. The results showed that the highest Cr absorption was found at 0,0050 mg/g, and the percentage of decolorization was 68,92% in the biosorbent ratio of 3:1 and pH 5. Biosorbent packed in the tea bags modification was effective removes Cr and dyes in the batik industry wastewater.

The effect of substrate composition on the activity of amylase and cellulase by Trichoderma harzianum strains under solid state fermentation Toga Pangihotan Napitupulu; Nathaniel Reinhart Silaban; Atit Kanti; I Made Sudiana
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Trichoderma harzianum is a filamentous fungus that has been known to have biocontrol and plant growth-promoting ability. However, the propagation of this fungus particularly through solid state fermentation (SSF) and characterization of its enzyme activity as one the indicator of quality of fermentation process are still needed to be explored further. Rice grain and its derivative products have economically as well as nutrient composition features beneficial as substrates of fungal propagation through SSF. Therefore, the aim of this study was to investigate the effect of white rice, rice bran, and combination of white rice and rice bran on the activity of amylase and cellulase by Trichoderma harzianum strains under SSF. Two strains of the fungus, InaCC F116 and InaCC F89, as well as their consortium were employed as fungal inoculants. After closed fermentation in dark chamber at 30 ± 1°C for 7 days, the activity of amylolytic as well as cellulolytic enzyme was assayed. The result showed that the presence of rice bran as a substrate increased the activity of crude amylase and crude cellulase. In all substrates, the strain F116 has low activity of both enzymes. The fungal consortium improves the activity of crude enzymes in all substrates. Therefore, the amylase and cellulase activity by T. harzianum in SSF condition were strain- as well as substrate-dependent.

Antagonistic activity of three Aspergillus isolates against Fusarium wilt of banana Iman Hidayat; Lita Citra Dewi; Dalia Sukmawati
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Endophytic fungi have been known to play a role in protecting their host plants against diseases and extreme environments. The purpose of this study was to determine the antagonistic activity of three Aspergillus isolates against F. oxysporum f. sp. cubense (Foc), and to identify the Aspergillus identity using multilocus phylogeny analysis. Antagonistic assay by dual culture method showed that the Aspergillus sp. strain PD2, strain PD4, and strain PD5 inhibited the growth of Foc isolate by 37.31%, 26.52%, and 12.04%, respectively. Multilocus phylogeny based on ITS rDNA, beta-tubulin, and calmodulin genes sequences showed that the Aspergillus strain PD2 and strain PD4 belong to A. section Terrei, while the Aspergillus sp. strain PD5 was identified as A. sydowii of the A. sect. Versicolores.

Inventory of macrofungi at peat swamp forest area, Kapuas Hulu, West Kalimantan Noverita Noverita; Tatang Mitra Setia
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

The areas surrounding peat swamp forest at Dusun Meliau and Bukit Peninjau, Kapuas Hulu, West Kalimantan host a diverse species of flora and fauna, including macrofungi. However, the information on the macrofungal diversity in this area has not been reported. The goal of this study was to obtain information on the diversity of macrofungi that can be used as food and medicine. Data was collected by direct modified collection method using plot (10 × 20 m) with interval 10 m along the transect line. A total of 79 specimens of macrofungi discovered from the two locations, of which 23 and 66 specimens were collected from Dusun Meliau and Bukit Peninjau, respectively. Of the 79 macrofungi specimens, 16 specimens were potential as a food source, and 41 as medicine. The macrofungi in this study was dominated by members of Ordo Aphyllophorales.

Cover JMSB Vol.1 No.2 (2019) Cover JMSB
Journal of Microbial Systematics and Biotechnology Vol 1, No 2 (2019): December 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

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Debora Christin Purbani

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+6221-8761356

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editor@e-jmsb.id

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Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia

Location
Kota bogor,

Jawa barat

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

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Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Debora Christin Purbani

Contact Email editor@e-jmsb.id

Phone +6221-8761356

Journal Mail Official editor@e-jmsb.id

Editorial Address Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia

Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Debora Christin Purbani

Contact Email
editor@e-jmsb.id

Phone
+6221-8761356

Journal Mail Official
editor@e-jmsb.id

Editorial Address
Jln. Raya Jakarta-Bogor KM. 46 Cibinong 16911, West Java, Indonesia

Location
Kota bogor,

Jawa barat

INDONESIA