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Debora Christin Purbani
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INDONESIA
Journal of Microbial Systematics and Biotechnology
Published by Cibinong Science Center
ISSN : -     EISSN : 26854430     DOI : https://doi.org/10.37604/jmsb
Core Subject : Science,
Immunology & microbiology
Journal of Microbial Systematics and Biotechnology (JMSB) is the international journal of microbial diversity and microbial technology which publishes research articles, reviews, and methodologies of microbial-based technology; and taxonomic articles such as monographs, new species, new notes, new records, checklists related to microbial diversity. The official language is English. Every manuscript submitted to JMSB will be published as soon as the editor receives it, and through the peer review process.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi
Articles 32 Documents
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Characterization and application of nitrogen-fixing and indole-3-acetic acid producing bacteria A13 in Oil Palm (Elaeisguineensis Jacq.) seedling Ismi Isti'anah; Nisa Rachmania Mubarik; Aris Tjahjoleksono
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.77

Abstract

Oil palm plantations have a good prospect in Indonesia. One of the efforts to improve the productivity of oil palm plantation is the application of bacteria as biological fertilizer. The research was conducted to characterize and apply the nitrogen-fixing and indole-3-acetic acid producing bacteria in oil palm seedlings. The bacteria was isolated from soil samples which taken from Taman Nasional Bukit Dua Belas (TNBD) Jambi. Nitrogen free bromthymol blue (NFB) is used as media for nitrogen-fixing bacterial isolation. Selected isolate named A13 had an ability to form white pellicle on the surface of the semisolid medium, increased the pH, and changed the color of medium from green to blue Isolate A13 was identified as Gram-negative bacteria and had a rods shape. Analysis of 16S rRNA gene sequence showed that isolate A13 had a similarity with Pseudochrobactrum assacharolyticum. Hypersensitivity assay on tobacco leaves showed that isolate A13 was not a pathogen. During 48 hours of incubation, isolate A13 produced a maximum of IAA at the 24th hour of incubation. Isolate A13 produced 0.675 ppm of ethylene/hour in Acetylene Reduction Assay and 69,839 ppm of IAA in HPLC methods. This was the first report on nitrogen fixation and IAA production by Pseudochrobactrum assacharolyticum and its application in the soil of oil palm seedlings. Application of isolate A13 in oil palm seedling increased significantly the number of lateral roots, stem diameter, and height of plants
Effect of Clove essential oil (Syzygium aromaticum) against the growth of dandruff scalps-causing fungal pathogen using Kirby- Bauer method in vitro Khusnul Khusnul; Puput Putuh Anisa; Dewi Peti Virgianti
Journal of Microbial Systematics and Biotechnology Vol 3, No 1 (2021): August 2021
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37604/jmsb.v3i1.78

Abstract

One of the strongest contributing factors in dandruff scalps is the presence of fungal pathogen or called as Malassezia. Several studies have found a connection between Malassezia and scalp health, one of which is caused by pathogenic fungal infections including Pityrosporum ovale, Microsporum gypseum, and Candida albicans. Clove flowers are known to contain eugenol and its derivative compounds which have antimicrobial, antifungal, antiseptic, and local anesthetic activity. This research aims to determine the effect of clove flower essential oil (Syzygium aromaticum) against some dandruff-causing fungi. The research method was to test the inhibitory power of clove flower essential oil using the diffusion method (Kirby Bauer technique). Samples of pathogenic fungi were cultured on SDA media (Saboroud Dextrose Agar) and given discs that had been given several concentrations of clove flower essential oil. The concentrations used were 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, positive control using 2% ketoconazole and negative control using virgin coconut oil (VCO). The results showed a significant effect between the ability of essential oils to inhibit the growth of some fungi that cause dandruff. The treatment of clove flower essential oil against M. gypseum at the concentration of 10% has an inhibitory ability of 33,05 mm and at the concentration of 100% amounted to 63.95 mm. Meanwhile, the inhibition percentage in P. ovale with a concentration of 10% and 100% of extract were 14,75 mm and 46,15 mm, respectively. The inhibition rate in C. albicans with the concentration of 10% extract and 100% of extract was 13,5 mm and 39,35 mm. The research shows that clove flower essential oil inhibits the growth of several pathogenic fungal in vitro.

Page 4 of 4 | Total Record : 32

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