CORD
Aims CORD aims to publish original research results and reviews on research and development. While encouraging those involved in research and developments to disseminate their finding it also assists policy makers and planners to be aware of the latest developments in the sector Scope CORD encompasses a broad range of research topics in animal sciences: seedlings and genetics, farming, harvesting, production, health, biotechnology, and production, socio-economic, and policy.
Articles
390 Documents
Origin, Domestication, Dissemination and Genetic Diversity of Coconut: DNA information
Lalith Perera
International Coconut Community Journal Vol 17 No 01 (2001): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v17i01.347
Information on origin, dissemination and levels and distribution of genetic diversity in coconuts will allow plant breeders and conservationists to select better breeding materials and formulate appropriate conservation strategies. PCR-based DNA profiling of coconut palms from Sri Lanka was initially conducted using both Amplified Fragment Length Polymorphism (AFLPs) and Microsatellites (SSRs). Thirty-nine microsatellite primers specific to coconut were developed by small insert genomic library construction. Eighteen of those primers were used to analyze the same set of Sri Lankan coconut materials. Overall, the results generated by both AFLPs and SSRs were in agreement. Most diversity was found in the tall variety (Typica) (0.92 and 0.62 for AFLPs and SSRs, respectively) rather than the intermediate (Aurantiaca) and dwarf (Nana) varieties (0.82 and 0.25 for AFLPs and SSRs, respectively). A hierarchical analysis of molecular variance (AMOVA) based on AFLP data was used to quantify and partition levels of variability between and within form components. This revealed that for the inbreeding dwarf and intermediate forms most variation was observed between rather than within forms. In contrast, the out-breeding tall forms exhibited as much variation within as between forms.
A NEW TYPE OF TRAP FOR CAPTURING ORYCTES RHINOCEROS (SCARABAEIDAE, DYNASTINAE), THE MAIN PEST IN YOUNG OIL PALM AND COCONUT PLANTINGS
Morin J.-P.;
Ps. Sudharto;
R. Purba;
R Desmier de Chenon;
T. Kakul;
S Laup;
L. Beaudoin-Ollivier;
D. Rochat
International Coconut Community Journal Vol 17 No 02 (2001): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v17i02.348
Oryctes rhinoceros (Scarabaeidae, Dynastinae) trapping is possible with the pheromone (ethyl 4-methyloctanoate) as the attractant. This article describes a new type of trap that functions without insecticides or water, and can remain in place for several months without any particular maintenance so long as the attractant does not run out. As it is 2 m tall, it offers the advantage of having the silhouette of a palm stem, which is visually attractive to this insect
BIOTECHNOLOGY IN COIR EXTRACTION & WASTE UTILIZATION
Anita Das Ravindranath
International Coconut Community Journal Vol 17 No 02 (2001): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v17i02.349
Coir, the fibre extracted from the mesocarp of the drupe of the coconut palm cocos nucifera is used for the production of beautiful products like mats, matting, carpet, handicrafts etc. (Table I) Coir suits very well for geotextiles and insulating materials and being environment friendly has an edge over synthetics for application in environmental operations.
DEVELOPMENT OF DAC INDIRECT ELISA FOR THE RAPID DETECTION OF COCONUT ROOT (WILT) DISEASE
M.Sasikala;
K.R.Chithra;
J.J.Solomon;
G.Rajeev
International Coconut Community Journal Vol 17 No 02 (2001): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v17i02.350
Identification of root(wilt) disease-free palm is a basic requirement for evolving disease resistant/tolerant planting materials for the management of this phytoplasma induced malady. For this purpose, various types of ELISA were performed with different enzymes and their respective substrates to standardize the most suitable and sensitive one. Indirect ELISA, Protein A indirect ELISA and F(ab')2 indirect ELISA using Alkaline Phosphatase conjugate and DAC indirect ELISA using Horse raddish peroxidase conjugate were carried out. Of the various types of ELISA tried, DAC indirect ELISA has been found to be the best for the rapid detection of coconut root(wilt) disease. In this assay, crude leaf extracts and unfractionated polyclonal antiserum were employed as test antigen and specific antibody respectively. Higher specificity was observed with the addition of gelatin and ovalbumin in the extraction medium and overnight incubation of ELISA plate at 5°C after substrate addition. Antigen titre was found to be very high in spear leaves followed by the next outer leaf. Similarly, maximum antigen titre was observed during the early stage of the diseased palm. The test could be completed within 44h and in a single ELISA plate, 20 samples with three replications could be screened using microlitre quantities of the specific antibody.
FACTORS INFLUENCING THE PRODUCTIVITY OF COCONUT ESTATES
T.S.G. Peiris;
M.T.N. Fernando;
U.P. de S. Waidayanatha
International Coconut Community Journal Vol 17 No 02 (2001): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v17i02.351
The yield gap between the potential and actual productivity has been widening in coconut estates (>9 ha) over the years. Among many reasons, the non-adoption and incorrect adoption of recommended agronomic and crop management practices on coconut cultivation have largely contributed to this yield gap. A survey carried out by the Coconut Research Institute (CRI) indicated that the lack of competent caretakers to correctly implement the crop management practices is a major reason to the yield gap and that there is an urgent demand for trained Farm Managers. Eight percent of the estates was identified as ‘poor’ with respect to the nut yield and the standard of the management practice adopted. Majority of both proprietors and caretakers (75%) was not satisfied with the productivity of their estates. The performance of the estates was significantly associated with the land suitability class of coconut and the uses of recommend practices. About 75% of the caretakers had not received any training on coconut cultivation. According to the perception of the proprietors, males between 25-30 years of age are the best group to be trained as Farm Managers. The training course should include theory and practice on all aspects of coconut cultivation, intercropping, animal production, labour management, accounting procedures in estates, and the use of agricultural machinery.
PHOTOSYNTHETIC ACCLIMATIZATION IN ZYGOTIC EMBRYO CULTURED PLANTLETS OF COCONUT (COCOS NUCIFERA L.)
S. Naresh Kumar;
V. Rajagopal;
Anitha Karun
International Coconut Community Journal Vol 17 No 02 (2001): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v17i02.352
It is important to understand the physiological acclimatization process in order to provide the favorable growing conditions, to increase the survival rate and to produce vigorous seedlings for field planting for better establishment. These aspects assume significance because the embryo culture technique is used for germplasm collection in coconut. The zygotic embryos from eight month old selfed nuts from West Coast Tall (WCT) cultivar of coconut were collected for embryo culture. The field planted seedlings produced by embryo culture and those raised from nursery were compared for photosynthetic activity under field grown conditions. Photosynthetic and water relation parameters were studied in the embryo cultured plantlets at different stages of acclimatization under green net house conditions. The photosynthetic rate increased gradually during acclimation with maximum rate atfour months after transfer to pots (under 50% shade; stage 4). The embryo cultured plantlets had higher photosynthetic rates compared to the nursery raised plants under field conditions. Transpiration rate increased with stage during acclimation. Embryo cultured plants had low transpiration rate as compared to the nursery raised plants. Consequently embrvo cultured plants had higher WUE (A/E). The WUE increased during acclimation. The Fv/Fm ratio increased gradually from 0.69 to 0.72 till stage 4. The field‑grown embryo culture plants had higher Fv/Fm compared to the nursery raised plant. This increase in Fv/Fm during acclimation indicated the increase in photochemical activity and photosystem II electron transport efficiency. The study indicated increase in photosystem efficiency and carbon assimilation efficiency of embryo cultured plantlets of coconut during acclimatization.
ELECTRON MICROSCOPIC EVIDENCE FOR PURIFICATION OF COCONUT ROOT (WILT) DISEASE PHYTOPLASMA
M. Mayilvaganan;
J. J. Solomon
International Coconut Community Journal Vol 18 No 01 (2002): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v18i01.353
Root (wilt) disease phytoplasma was purified from diseased coconut tissues using discontinuous Percoll gradient centrifugation method. Crude sap prepared from coconut was layered on a discontinuous Percoll gradient of 15, 30, 50 and 60%(v/v). After centrifugation at 20,000 g for 30 min, the turbid fraction formed on the top of 30% gradient in the diseased plant material was recovered, processed and fixed for electron microscopy. Electron microscopic examination of sections prepared from purified preparation of diseased plant material showed typical cells of root (wilt) phytoplasma with heterogeneous sizes and more or less spherical shape that are similar to those found in sieve elements of diseased tissues and salivary glands of infective (viruliforms) insect vectors. These purified phytoplasma bodies showed trilaminar membrane with internal materials of ribosome granules and DNA fibrils. However, the yield in terms of number of cells was fewer and in addition to intact bodies, free membranes and empty bodies lacking internal contents also were observed.
EXTENT OF DAMAGE TO COCONUT PALMS CAUSED BY SUPER CYCLONE IN ORISSA AND PATTERN OF THEIR RECOVERY
D.K. Dash;
D.P. Ray;
H. Hameed Khan
International Coconut Community Journal Vol 18 No 01 (2002): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v18i01.354
During the Super cyclone (October, 1999) in the coastal districts of Orissa, 26,836 hectares of coconut garden having 46,96,300 palms were severely affected. A survey of affected palms in the selected villages classified the palms as (i) uprooted (11.3 percent) (ii) palms with complete crown damage (23.3 percent) (iii) palms with severe damage to the crown (32 percent) and (iv) palms with partial crown damage (34 percent). The affected palms lost most of their valuable leaves and spadices in their varying stages of development. The recovery of palms started after 2 months of damage in the form of production of new leaves. The spadices production started after 4-5 months of damage. The number of palms entered the fresh reproductive stage is of low percent, which may lead to in 2-3 years delay in nut production by the palm. Regular manuring with organics and inorganics, adequate plant protection are suggested to rejuvenate the palms.
PERFORMANCE OF SELECTED COCONUT VARIETIES/ HYBRIDS UNDER SEQUENTIAL COCONUT TODDY AND NUT PRODUCTION SCHEME OR SCTNP
M.I. Secretaria;
R.M. Ebuna;
S.S. Magat;
J.N. Maravilla;
G.A. Santos;
G.B. Baylon
International Coconut Community Journal Vol 18 No 01 (2002): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v18i01.355
The response to sequential coconut toddy and nut production (SCTNP) scheme of four coconut varieties/hybrids at PCA-Zamboanga Research Center, San Ramon, Zamboanga City showed that the dwarf x tall hybrids, Malayan red dwarf x Baybay tall (MRD x BAYT), Malayan red dwarf x Tagnanan tall (MRD x TAGT) and Catigan green dwarf x Laguna tall (CATD x LAGT) produced significantly higher toddy (495 – 699 li/tree/yr) and nut yield (143-169 nuts/tree/yr) compared to tall cultivar, ‘Laguna’ LAGT (364 li and 123 nuts per tree per year). Varietal difference in terms of the floral and vegetative characteristics of the coconut cultivar and hybrids contributed significantly to the differences in the sap/toddy and nut yields.
USEFULNESS OF EARLY DIAGNOSTIC METHODS FOR EFFECTIVE MANAGEMENT OF BASAL STEM ROT (GANODERMA) DISEASE OF COCONUT
A. Karthikeyan;
S. Mohan;
R. Bhaskaran
International Coconut Community Journal Vol 18 No 01 (2002): CORD
Publisher : International Coconut Community
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DOI: 10.37833/cord.v18i01.356
Chemodiagnostic methos EDTA and TTC tests and physiological parameters electrical conductivity and relative water content are useful to detect the basal stem rot disease in coconut palms, 4 - 14 months before visual symptom expression. The critical values of these tests for symptom expression have been fixed. In coconut trees, root sampling from any directions at 15 - 30 cm depth is optimum for early detection tests. Coconut palms without symptoms but showing higher values in diagnostic tests responded well to treatments by registering lesser disease index and higher nut yield than the treated palms with external symptoms.