Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Conservative Dentistry Journal

CDJ
Website

Published by Universitas Airlangga

ISSN : 20871848 EISSN : 27228045 DOI : 10.20473/cdj.v10i1.2020.1-4

Core Subject : Health,

Dentistry

Journal of conservative dentistry accepts original manuscripts in the field of Endodontic other related subjects articles, including research, case reports and literature reviews. The spread of fields include: Endodontic research; Preventive, curative and rehabilitative related to endodontic field; Oral health education and promotion related to endodontic field; Endodontic and restorative clinical research; Basic sciences related to endodontic field; Endodontic healthcare management

Arjuna Subject : Kedokteran Gigi - Kedokteran Gigi (Lain-Lain)

Articles 10 Documents

Search results for , issue "Vol. 9 No. 1 (2019): January - June" : 10 Documents clear

PERBEDAAN ANGIOGENESIS PADA PULPA SETELAH APLIKASI EKSTRAK PROPOLIS DAN KALSIUM HIDROKSIDA Camelia Ariesdyanata; Cecilia G.J. Lunardhi; Agus Subiwahjudi
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background : Propolis is a resinous hive product collected by bees ( Apis Mellifera ) from tree buds and mixed with secreted bee wax in order to avoid bacterial contamination in the hive, and also to seal it. Propolis is employed for the treatment of various infectious diseases because it is wellknown that is has antibacterial and anti-inflammatory properties. Calcium hydroxide was introduced to the dental profession in 1921 and has been considered the “gold standard” for direct pulp capping materials in the past decades. Aims :This research is to investigate the development of new blood vessels ( angiogenesis ) in rat's dental pulp following application of propolis extract and calcium hydroxide. Methods : There was 43 Strain Wistar rats of 8–16 week old and 200–250 grams in weight were used in this study. The rats were randomly divided into 6 groups. Pulp exposures were performed on the occlusal surface of right maxillary first molars. At the 1st and 4th groups, as the control group, without pulp capping paste. At the 2rd and 5th groups, pulp exposure was applied with propolis extract. And at 3th and 6th groups pulp exposure was applied with calcium hydroxide ,and the 7th group is negative control is a normal teeth. Pulp exposure was applied with propolis extract. After that, all of the cavities were filled with light cured glass ionomer cement as a permanent filling. Animals on the 1st, 2rd, and 3th groups were decaputed after 7x24 hour post pulp capping material application and were sent for histological examination which new blood vessels ( angiogenesis ) cells were present. And at the 4th, 5th, 6th groups were culled after 7x 24 hour post pulp capping material application and were sent for histological examination which new blood vessels ( angiogenesis ) evaluated were present. Result : All sample were histopathological examinated and data was statiscally analysed using one way ANOVA the histological analysis revealed that the development of the new blood vessels occurred in all group. The new blood vessels ( angiogenesis ) of propolis extract group was milder compared to the control and calcium hydroxide group, with statistical analysis showed significant difference (p > 0.05). Conclusion: The development of new blood vessels is earlier happened in group capping material containing propolis and which show with reduce the amount of the new blood vessels in days 7 and 14 than the other group.

Antibakteri Ekstrak Kulit Buah Kakao (Theobroma cacao) 6,25% dan NaOCl 2,5% Terhadap Bakteri Streptococcus sanguinis Sepdhyo Wahyu Nugroho; Mandojo Rukmo; Edhie Arief Prasetyo; Tamara Yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Streptococcus sanguinis is a gram-positive bacterium that infects and penetrates into dentinal tubules from a depth of 150 μm up to 792 μm. Chemicals, namely 2.5% NaOCl, are used to mitigate the growth of these bacteria via irrigation of the root canals, but 2.5% NaOCl still has disadvantages including irritating periradicular tissue, having an unpleasant odor, and being toxic. Due to these shortcomings, natural materials are expected to be used as alternatives. Cocoa peel extract has active tannin compounds, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial attributes; a concentration of 6.25% is counted as the minimum bactericidal concentration (MBC) of Streptococcus sanguinis. Aim: To compare the difference of antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) and 2.5% NaOCl against Streptococcus sanguinis. Method: This research is an in vitro experimental laboratory with post-test only control group design. The diffusion method was used on Streptococcus sanguinis planted in tubes containing BHIB, then cultured on petri dishes containing nutrient agar and divided into 3 parts, namely 6.25% cocoa peel extract, 2.5% NaOCl, and negative controls, then each Petri dishes were given a paper disc that had been dripped by 0.01 ml of each ingredient, then incubated in an incubator for 2x24 hours at 37°C; the diameter of the inhibitory zone formed was then observed using a caliper. Results: The average diameter of the inhibition zone formed on 6.25% cocoa peel extract was 19.2000 mm and 2.5% NaOCl was 17.2813 mm against Streptococcus sanguinis. Conclusion: The antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) is higher than 2.5% NaOCl against Streptococcus sanguinis.

Sitotoksisitas Ekstrak Kulit Kakao (Theobroma cacao) terhadap Kultur Sel Fibroblas BHK-21 Fajariana Fitriani; Adioro Soetojo; Agus Subiwahjudi; tamara yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Latar Belakang: Irigasi saluran akar merupakan salah satu tahapan penting untuk menunjang keberhasilan perawatan. Sodium hipoklorit (NaOCl) merupakan larutan irigasi utama yang sering digunakan namun memiliki sejumlah kekurangan yakni bersifat toksik jika diirigasi sampai ke jaringan periradikular. Kakao (Theobroma cacao) merupakan tumbuhan suku Sterculiaeae yang kulit buahnya mengandung senyawa alkaloid, flavonoid, tanin, saponin dan terpenoid. Senyawa-senyawa tersebut terbukti dapat menghambat pembentukan biofilm pada bakteri Enterococcus faecalis yang merupakan bakteri paling resisten pada saluran akar. Ekstrak kulit kakao diharapkan dapat menjadi bahan alternatif irigasi saluran akar yang ideal, sehingga perlu dilakukan penelitian mengenai efek sitotoksisitasnya terhadap jaringan. Tujuan: Menentukan konsentrasi dari ekstrak kulit kakao yang memberikan efek sitotoksik pada sel fibroblas BHK-21. Metode: Jenis penelitian yang dilakukan adalah eksperimental laboratoris dengan menggunakan kultur sel fibroblas BHK-21. Ekstrak kulit kakao diperoleh melalui maserasi menggunakan etanol 70% dan dilakukan pengenceran dengan konsentrasi 1,56%, 3,125%, 6,125%, 12,5%, 25%, 50%, dan 100%. Sel BHK-21 dalam microplate 96 well dipaparkan dengan ekstrak kulit kakao. Uji sitotoksisitas menggunakan MTT assay dan absorbansi warna dibaca menggunakan Elisa reader. Nilai absorbansi dihitung dengan rumus sehingga didapatkan hasil akhir berupa persentase kematian sel. Hasil: Peningkatan konsentrasi ekstrak kulit kakao berbanding lurus dengan kenaikan persentase sel fibroblas BHK-21 yang mati. Kesimpulan: Konsentrasi minimum ekstrak kulit kakao yang dapat memberikan efek sitotoksik pada sel fibroblas BHK-21 adalah 6,25%.

Antibacterial power effectiveness of calcium hydroxide and propolis mixture on Fusobacterium nucleatum bacteria ira Widjiastuti; S. Sukaton; Agnes Melinda Wong; Nanik Zubaidah
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide is a root canal dressing material that is widely used in dentistry because of its ability to regenerate hard tissue and eliminate bacteria. According to Ferreira et al. (2015), Fusobacterium nucleatum was found in 90% of teeth that had a root canal treatment done using calcium hydroxide as an intracanal medication. Due to this shortcoming of the antibacterial power of calcium hydroxide, additional research on alternative ingredients that can be combined with calcium hydroxide to improve its antibacterial power is necessary. Propolis is a natural material that has high antibacterial power and has long been used in dentistry. The addition of propolis to calcium hydroxide is expected to improve the antibacterial power of calcium hydroxide without eliminating its function in terms of regenerating hard tissue. Purpose: To find out how effective is the antibacterial power of a combination of calcium hydroxide and propolis against Fusobacterium nucleatum. Methods: The research was carried out using 4 treatment groups consisting of 6 samples for each group. Group 1 is given a combination of calcium hydroxide and propolis with a ratio of 1:1, group 2 with a ratio of 1:1.5, group 3 with a ratio of 1:2, and group 4 is a positive control of calcium hydroxide and sterile aquadest suspension. Each sample was put into a test tube containing BHIB and a suspension of Fusobacterium nucleatum, incubated at 37ºC for 24 hours, and vortexed for 1 minute. A total of 0.1 ml of bacterial inoculum was taken from each sample and then put into the MHA and grown for 24 hours. The number of Fusobacterium nucleatum colonies grown on MHA was calculated and expressed using the Colony Forming Unit (CFU). Results: There were fewer colonies of Fusobacterium nucleatum in the treatment group compared to the control group. Conclusion: The combination of calcium hydroxide and propolis has an effective antibacterial power against Fusobacterium nucleatum which the ratio of 1:2 is more effective than ratio of 1:1,5 and 1:1.

Effect of 405 nm Diode Laser with Varying Irradiation Time on BHK-21 Fibroblast Viability Kun Ismiyatin; Leidy Herlin Rumbiak; Widya Saraswati; Sri Kunarti; Anuj Bhardwaj
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation; it can be used for surgery, detoxification, bio stimulation and antibacterial. However, lasers have Biphasic Dose Response (BDR), which is bio stimulation and bio inhibition. To determine if 405 nm laser diode is biocompatible, viability test is necessary before these lasers can be labeled as viable to use in dental therapy. Aim: To prove the variation of radiation time of the 405 nm laser diode radiation can cause bio stimulation and bio inhibition response that affects the viability of BHK-21 fibroblast cells. Method: Viability test was carried out using BHK-21 fibroblast cells which were inserted into 96-well microplate, then radiated with 405 nm laser diode with varying irradiation time of 30s, 60s, 120s, 240s and 480s. After radiation, the cells are then incubated for 24h. Cytotoxicity was observed using MTT assay and ELISA reader. Data was analyzed using the Kolmogorov-Smirnov test, Levene Test, Welch ANOVA, and Tukey HSD. Results: BHK-21 fibroblast cells radiated with 405 nm laser diode with radiation time of 30s, 60s, 120s, and 240s have the same viability as the control cell, while at 480 seconds the viability exceeds that of the control cell. Conclusion: 405 nm laser diode with radiation times of 30s, 60s, 120s, and 240s do not affect the viability of BHK-21 fibroblast cells. Meanwhile, 480s irradiation time of 405 nm laser diode causes bio stimulation response that increases the viability of BHK-21 fibroblast cells.

EFFECTIVENESS OF TIME IRRADIATION BY LASER DIODE 650 NM AND PHOTOSENSITIZER Methylene Blue ON DECREASING THE NUMBER OF MIXED BACTERIAL COLONIES FROM NECROTIC ROOT CANAL Nadya Ayusandra Larasati; Kun Ismiyatin; Sri Kunarti
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background. Pulp necrosis is defined as the irreversible death of pulp tissue. It clinically observed by the destruction of its own tissue. The primary etiology of pulp necrosis is irritation due to bacterial infection. The treatment for pulp necrosis is root canal treatment with a success percentage ranging from 40-93% based on cavities with minimal bacteria that can be sterilized. One method of sterilization using Antibacterial Photodynamic Therapy (aPDT), which uses laser diodes is currently being developed in the field of conservative dentistry, but there are still many differences of opinion regarding the ideal amount of time of laser radiation or photoactivation used to reduce the number of bacteria, especially in root canals. Aim. To determine the effective radiation time of the 650 nm wavelength laser diode with Methylene Blue Photosensitizer in reducing the number of bacterial colonies from necrotic root canals. Method. This research is a laboratory experimental study with 30 samples and 6 groups with different durations of irradiation namely 45, 60, 75 and 90 seconds. Results. It was found that there was a decrease in the number of mixed bacterial colonies within necrotic root canals with the obtained p-value of the ANOVA test results being <0.05. This shows that there is a significant difference between the amounts of mixed bacterial colonies from necrotic root canals in each treatment group. Conclusion. Diode laser radiation with a wavelength of 650 nm with duration of 90 seconds of radiation and Methylene Blue Photosensitizer is an effective time in reducing the number of bacterial colonies from necrotic teeth compared to the duration of radiation of 45, 60 and 75 seconds.

Effect of Different Radiation Times on the Antibacterial Ability of Laser Diodes (650 nm) on Streptococcus mutans Sri Kunarti; S. Sukaton; Ajeng Hayyuning Citrasari
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Streptococccus mutans is an acid-producing gram-positive bacterium that colonizes the tooth surface and causes damage to the hard tissue of the tooth. S. mutans is known as the main agent that causes caries. Photodynamic therapy (PDT) consisting of photosensitizers and a light source, such as a laser beam, is considered to have an antibacterial effect on S. mutans. However, the factors that influence the antibacterial effects of the lasers, such as the amount of energy, wavelength, use of photosensitizer, and the duration of radiation still need to be studied. Aim: To determine the effective time(duration) of 650 nm laser diode radiation as an antibacterial agent against S. mutans after 30, 45, 60, and 75 seconds of radiation. Method: 30 samples were divided into 6 groups; (1) S. mutans without methylene blue (MB) and laser, (2) S. mutans with MB, but without a laser, (3) S. mutans with MB and laser for 30 seconds, (4) 45 seconds, (5) 60 seconds, and (6) 75 seconds. After treatment, all samples were cultured and incubated for 48 hours then colony counts were carried out in each group. The results were analyzed using ANOVA and Tukey HSD Test with a p value of <0.05. Results: The ANOVA and Tukey HSD test showed a significant difference in each group. Conclusion: 650 nm laser diode radiation with a duration of 75 seconds is an effective time as an antibacterial against S. mutans compared to 30, 45 and 60 seconds.

The Difference Of Antibacterial Power Between Cocoa Peel (Theobroma cacao L.) Extract 6,25% and Chlorhexidine 0,2% Againts Streptococcus sanguinis Nila Murni Wulandari; Edhie Arif Prasetyo; Agus Subiwahjudi; Tamara Yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Streptococcus sanguinis is a bacterium that can cause failures in root canal treatments due its ability to penetrate the dentinal tubules to a depth of 400 μm in just two weeks. Irrigation material is needed to stop the growth of this bacteria so that no bacteria can pass through by using chemicals, irrigation materials that are widely used such as Chlorhexidine 0,2% but still lack because it cannot be used as a single irrigation solution because its effectiveness will be reduced if it is related to protein and organic dentine matrix and low Ph saliva.Therefore, research is needed to find natural ingredient that can be an alternative such as Cocoa peel extract was chosen because it contains active compounds, in the form of saponins, tanins, alkaloids, flavonoids, and terpenoids that have been known to have antibacterial properties a concentration of 6,25% is used in accordance with the MKC of Streptococcus sanguinis. Aim: To compare the antibacterial power between cocoa peel extract (Theobroma cacao L.) 6,25% and Chlorhexidine 0,2% against Streptococcus sanguinis Method: This research is an experimental laboratory with a post-test only control group design. The diffusion method was used to determine the susceptibility of bacteria isolated from the material by planting the culture of Streptococcus sanguinis on the agar medium by swabbing the nutrient media which has been divided into 3 parts consisting of negative control, cocoa peel extract and Chlorhexidine, then each nurient media so that it is given a paper disk and 0.01ml liquid on each section. The diameter of the inhibition zone was observed after 2x24 hours using the calipers. Results: The average inhibitory zone that was formed using cocoa peel extract was 20,40 mm against Streptococcus sanguinis and Chlorhexidine was 18,36 against Streptococcus sanguinis. Conclusion: Cocoa peel extract (Theobroma cacao L.) 6,25% had higher anti-bacterial power compared to 0,2% Chlorhexidine against the growth of Streptococcus sanguinis.

Compressive Strength Test on Calcium Hydroxide with Propolis Combination Ira Widjiastuti; S. Setyabudi; M. Mudjiono; Erika Setyowati
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide is a dental material used as a gold standard for pulp capping materials. However, calcium hydroxide has several weaknesses which cause many researchers to look for alternative ingredients that come from nature. Propolis in the field of dentistry has long been used because of its ability as an anti-inflammatory, anti-microbial, anti-fungal, and can cure scars. The combination of calcium hydroxide and propolis is proven to have good biocompatibility and anti-bacterial properties. One of the requirements of pulp capping material is to have sufficient compressive strength. Therefore, a research to test the compressive strength value of the combination of calcium hydroxide with propolis is needed. Purpose: To find out the difference in compressive strength of the combination of calcium hydroxide-propolis with a ratio of 1: 1, 1: 1.5, and 1: 2. Methods: The study used 4 treatment groups with each group consisting of 6 replications. Group 1 is a combination of calcium hydroxide-propolis with a ratio of 1: 1, group 2 with a ratio of 1: 1.5, group 3 with a ratio of 1: 2, and a positive control group using calcium hydroxide - sterile aquadest. Calcium hydroxide powder and propolis extract liquid is mixed according to comparison and printed on a cylindrical mold with the size of 4 mm x 6 mm. Then, the compressive strength was tested using an Autograph test instrument. Result: Compressive strength was smaller in group 3 compared to group 2, group 2 compared to group 1, and group 1 compared to the control group. Conclusion: In calcium hydroxide-propolis combination, the more propolis extract used in the combination the lower the compressive strength of the combination will be.

ANTIBACTERICAL POTENCY OF CALCIUM HYDROXIDE AND PROPOLIS MIXTURE ON MIXED BACTERIA OF CARIOUS DENTIN Ruslan Effendy; Vita Ariesta; Widya Saraswati
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide has been known as the gold standard of pulp capping process, but still has some weaknesses such as a pH that is too high to be potentially toxic, resistant to certain bacteria, causing necrosis of the pulp, and causing a tunnel defect. This weakness causes researchers to look for alternative ingredients that have antibacterical properties that can be combined with calcium hydroxide. Propolis has antibacterial properties such as flavonoids, tannins, saponins, and terpenoids which can work as the best carrier that allows dissociation of ions from calcium hydroxide so the combination of both that has antibacterial power is expected to have an effective antibacterial potency against the number of bacterial colonization with mixed dentine carious. Aims: This study aims to determine the antibacterial potency produced by a combination of calcium hydroxide and propolis against mixed bacteria of carious dentine. Methods: The research was carried out using 4 treatment groups with each of 7 samples. Group I is a combination of calcium hydroxide and propolis with a ratio of 1: 1, group 2 with a ratio of 1: 1.5, group 3 with a ratio of 1: 2, and a positive control group. Each sample was put into a suspense of mixed bacteria of carious dentine, grown for 24 hours. Suspense is vortexed for 1 minute so the solution becomes homogeneous. Put 0.1 ml of suspended mixed bacteria of dentine carious from BHIB media, grown for 24 hours on MHA media and counted the number of colonies. Results: The highest number of colonies in the positive control group, then group 1, group 2, and the least number of colonies in group 3 with the most extract of propolis. Conclusion: The combination of calcium hydroxide and propolis with a ratio of 1: 2 has the best antibacterial power in inhibiting colonization of mixed bacteria of carious dentine.

Page 1 of 1 | Total Record : 10

Filter by Year

2019 2019

Filter By Issues

All Issue Vol. 15 No. 2 (2025): July-December Vol. 15 No. 1 (2025): January-June Vol. 14 No. 2 (2024): July-December Vol. 14 No. 1 (2024): January-June Vol. 13 No. 2 (2023): July-December Vol. 13 No. 1 (2023): January-June Vol. 12 No. 2 (2022): July-December Vol. 12 No. 1 (2022): January-June Vol. 11 No. 2 (2021): July - December Vol. 11 No. 1 (2021): January-June Vol. 10 No. 2 (2020): July-December Vol. 10 No. 1 (2020): January-June Vol. 9 No. 2 (2019): July - December Vol. 9 No. 1 (2019): January - June Vol. 8 No. 2 (2018): July - December Vol. 8 No. 1 (2018): January - June Vol. 7 No. 2 (2017): July - December Vol. 7 No. 1 (2017): January - June Vol. 6 No. 2 (2016): July - December More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Dian Agustin W

Contact Email
cdj@journal.unair.ac.id

Phone
+62315030255

Journal Mail Official
cdj@journal.unair.ac.id

Editorial Address
DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132

Location
Kota surabaya,

Jawa timur

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Dian Agustin W

Contact Email cdj@journal.unair.ac.id

Phone +62315030255

Journal Mail Official cdj@journal.unair.ac.id

Editorial Address DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132

Location Kota surabaya, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Dian Agustin W

Contact Email
cdj@journal.unair.ac.id

Phone
+62315030255

Journal Mail Official
cdj@journal.unair.ac.id

Editorial Address
DEPARTEMEN KONSERVASI GIGI. FAKULTAS KEDOKTERAN GIGI, UNIVERSITAS AIRLANGGA PROF. DR. MOESTOPO 47. SURABAYA. 60132

Location
Kota surabaya,

Jawa timur

INDONESIA