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Contact Name
Andri Rahman
Contact Email
andri.agus.rahman@lipi.go.id
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Journal Mail Official
widyariset@mail.lipi.go.id
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Pusbindiklat LIPI, Jl. Raya Bogor Km. 46, Cibinong Science Center, Cibinong - Bogor 16911
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Kab. bogor,
Jawa barat
INDONESIA
Articles 476 Documents
TRANSFORMASI GEN ARABIDOPSIS ASYMETRIC LEAVES2 (AS2) PADA ANGGREK PHALAENOPSIS AMABILIS (L.) Blume DENGAN MEDIATOR AGROBAKTERIUM TUMEFACIENS Vincentia Esti Windiastri; Endang Serniarti
Widyariset Vol 12, No 1 (2009): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (680.877 KB) | DOI: 10.14203/widyariset.12.1.2009.155-164

Abstract

The Asymmetric leaves2 (AS2) gene is an important gene of a model plant, Arabidopsis thaliana, that involved in leaf development. To understand the molecular mechanism of leaf development in orchid, we transformed the AS2 gene into the Phalaenopsis amabilis orchid protocorms (developing orchid embryos). Agrobacterium tumefaciens LBA4404 that contained T-DNA with 355:: AS2 construct and pGreen vector were used for transformation. The-21-day-old protocorms that co-cultivated with Agrobacterium culture containing pG35S::AS2/LBA 4404 and pG35S/LBA4404 were maintained on 100 mg/1 kanamycin containing medium. The growing plants were selected as candidate of transformants. The putative transformants were analysed by PCR with a pair of AS2 gene specific primers (ORF15 F2 and ORF I 5 R2), that amplifying 600 by fragment of AS2 gene. The result showed that 79 plants out of 7966 protocorms transformant p35S: : AS2 and 64 plants out of 4622 protocorms transformant pG35S were kanamycin resistants, the efficiency of transformations were 0.99% and 1.38%, respectively. Phenotype characterization of the transformation showed that they produced various abnormal leaves shapes: oval (34.18%), wildtype-like (30.38%), rectangular (26.58%), lobed (3.80%), fused (2.53%) and trumpet (2.53%). These data indicate that abnormal shape of leaves might be due to the expressions of AS2 transgene in P amabilis.   
LINKAGE BETWEEN THE USE OF HIRED LABOR AND THE AMOUNT OF ESTICIDE APPLICATION AMONG TANGERINE FARMERS IN THAILAND: A LESSON FOR INDONESIA Evi Irawan
Widyariset Vol 14, No 2 (2011): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.241 KB) | DOI: 10.14203/widyariset.14.2.2011.275-284

Abstract

Labor is often neglected in the studies of adoption of agricultural innovation even though it may hinder or facilitate the adoption. This research is to analyze the linkage between the use of hired labor and the amount of pesticides application based on a survey data of 160 tangerine farmers in Thailand. The result shows that there is a positive signifi cant linkage between the use of hired labor and the amount of pesticide application. Main policy implication of this fi nding, especially in the Indonesian context, is that the target of integrated pest management extension or farmers fi eld class should be broadened to include farmers as well as hired laborers.
LOCAL GOVERNMENT INTERVENTION ON STREET LEVEL BUREAUCRACY OF BASIC HEALTH PROVISION IN DECENTRALIZED ERA Arief Priyo Nugroho
Widyariset Vol 15, No 1 (2012): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.919 KB) | DOI: 10.14203/widyariset.15.1.2012.207–216

Abstract

In health care provision, puskesmas (Community Health Center) can be described as street level bureaucracythat has important role in improving health services. The study aimed to analyze political intervetion inhealth service provision. This study used Michael Lipsky theory about street level bureaucracy and Karl Marxtheory about class and state to analyze pattern of intervention. This case study research was conducted in SouthKrembangan, Surabaya. Observation and secondary data analysis are the main tools to trace the pattern of localgovernment intervention in primary health care at puskesmas. The result shows that local government has beenbeing admitted indirect intervention in health service provision. The intervention was proved by the puskesmastreatment that referred to implement curative program from promotive and preventive that was actually its dutyand main function.
STUDI RELASI HIDRODINAMIKA SUNGAI CIWALEN DAN AIR TANAH MELALUI PENDEKATAN MODEL (Studi Kasus pada Aliran Sungai Ciwalen Kecamatan Garut Kota) Asep Mulyono
Widyariset Vol 13, No 2 (2010): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.464 KB) | DOI: 10.14203/widyariset.13.2.2010.75-81

Abstract

Ciwalen River flows pass of leather industry in Sukaregang area that is the one of the prominent industry in Garut region. The usage of chemicals in the leather tanning process resulted in toxic liquid waste released to Ciwalen River. Ciwalen River containing contaminants would further contaminate the groundwater system along the Ciwalen River. The study of groundwater flow particularly hydrodynamic relation river and groundwater surrounding is required. Field and secondary data were utilized in this study. Groundwater flow and hydrodynamic relation river and groundwater analyses were carried out using Visual Modflow v3.1. software. The study results that hydrodynamic relationship between Ciwalen River and the groundwater system can be divided into 6 segments. Segment 1, 4, and 6 belong to effluent type, while segment 2 is of influent type. Segment 3 and 5 located right of the river are effluent and those located in the left of the river are of influent type. River channel length of segment 1 are 0.95 km, length of segment 2 are 0.62 km, length of segment 3 are 0.88 km, length of segment 4 are 0.21 km, length of segment 5 are 0.63 km and length of segment 6 are 1.18 km. Influent type indicate causes a groundwater pollution because river that’s containing contaminants would further contaminate the roundwater system particularly at urban wells in surrounding segment.
DUPLICATION IN INDONESIAN JOURNAL ARTICLE: QUALITY ANALYSIS Ekawati Marlina; Retno Asihanti Setiorini; Kamariah Tambunan
Widyariset Vol 18, No 1 (2015): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.678 KB) | DOI: 10.14203/widyariset.18.1.2015.115–126

Abstract

There are several cases of duplicate articles in Indonesian scientific journal. This study provide inputs tothe journal publisher in Indonesia to prevent duplication. This paper conducted analysis of quality articles andjournals, data obtained from the Indonesian Scientific Journal Database (ISJD) and printed journals in PDIILIPI. The results show (1) the duplication are written by the same author, but published in different journals, in thesame or different year; (2) articles published in the same journal but in different volume; (3) articles from a similarresearch team, but published as sole author under the name of each member of the group. Most journals alreadyinclude a requirement that the article submitted has not been published in other journals. This paper suggests inorder to avoid duplication, each journal should add the prerequisite, such as authors should not submit the articleto other publishers before the selection process has been completed. In addition, to address duplication, whilethe selection is in process, publisher should conduct a benchmarking script analysis to search articles that havebeen published. ISJD, is a database for comparing articles and managed by PDII LIPI. Currently, ISJD alreadymanages more than 5,000 journals with more than 140,000 articles. This study is expected to provide input to thejournal publisher in Indonesia, so there is no duplication of publication of the article.
FLEXIBILITY VERBA(L) PREFFIX ME- IN KUBU LANGUAGE IN JAMBI PROVINCE: STUDY OF THE STRUCTURE AND SEMANTIC ristanto ristanto
Widyariset Vol 18, No 2 (2015): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.345 KB) | DOI: 10.14203/widyariset.18.2.2015.155-168

Abstract

This study discusses flexibility verb(l) prefixes the Kubu language consisting of (1) the symptoms morphophonemic verb(l) prefixes me- in Kubu language, (2) categories which can join the verb(l) prefixes me-, and (3 ) grammatical meaning that is generated by of the prefix. The research use method descriptive. Methods of assessment in the form of distributional element techniques for direct, deletion, extension, substitution, and insertion. Data is taken from of their speaking and writing. This study took place in Sarolangun, Province of Jambi. The results of this study indicate that the categories that can be joined with the verb(l) prefixes me- in Kubu language is the form of (1) verb, (2) noun, (3) adjectives, and (4) numeral. Prefix me- is experiencing into a process morphophonemic meng-, m-, men-, mem-, meny-, and mong-. Grammatical meaning of the verb(l) prefixes me- in Kubu language are in the form of the ‘causative’ meaning and signifying ‘doing’.
COSINE ERROR REDUCTION ON THE UNIVERSAL MEASURING MACHINE CALIBRATION USING DISPLACEMENT LASER INTERFEROMETER Ardi Rahman
Widyariset Vol 15, No 3 (2012): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (513.414 KB) | DOI: 10.14203/widyariset.15.3.2012.657–664

Abstract

The calibration is important for many people as it can guarantee the traceability of measurement. Universalmeasuring machine is one of the coordinate measuring machine which is capable to calibrate the amount ofdimensional measurement standards, while, the UMM was calibrated by displacement laser interferometer. Inthe UMM calibration, cosine error was occurred, where the measurement result value is greater than the actualdimension value. The aim of this research was to reduce the cosine error in the UMM calibration using the DLIby adjusting the DLI optics. The results showed that the cosine error was decreased 10 μm compared to the 2006calibration data, and the uncertainty of measurement decrease of 61.5%.
UNGKAPAN IDIOMATIS DALAM BUDAYA MASYARAKAT MINANGKABAU Fairul Zabadi
Widyariset Vol 12, No 3 (2009): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.808 KB) | DOI: 10.14203/widyariset.12.3.2009.53-60

Abstract

The objective of this research is to find out some idiomatic expressions in Minangkabau viewedf rom aspect form and meaning. The method used in this study is qualitative based on content analysis technique. Research findings are 27 idioms which consist of verbal idioms and nominal idioms. Verbal idioms may appear in (1) theconstruction VERB + NOUN as examplified by malapeh ao `to get no benefit' and (2) the contruction ADVERB + VERB as examplified by alah ilang 'to die'. Meanwhile, the construction of nominal idioms are three types: (1) NOUN + NOUN as examplified by kaeang miang fink, (2) NOUN + ADJECTIVE as exemplified kabau gadang'lazybones', and (3) ADJECTIVE + NOUN as examplified gadang kalang `coward'.  
THE EFFECT OF INTERCROPPING MODEL AGAINTS WHEAT AND TABACCO GROWTH AND PRODUCTION Alfna Handayani
Widyariset Vol 14, No 3 (2011): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.012 KB) | DOI: 10.14203/widyariset.14.3.2011.479-488

Abstract

The aim of this research was to study the effect of intercropping model against wheat and tobacco pro-duction. The experiment was conducted in Kaliangrik-Kabupaten Magelang, Samiran-Kabupaten Boyolali, Kledung-Kabupaten Temanggung from April 2010 to October 2010.The experiment was arranged in Random-ized Completed Block Desain consisted of 3x3+2 control with 5 replications. The fi rst factor were 3 location (Kaliangkrik, Samiran, Kledung). The second factor were 3 model wheat-tobacco intercropping, i.e: model I: tobacco population is constant with wheat planted in two other sides of tobacco, model II Tobacco population is constant with wheat planted only in one side, model III tobacco population is decreased a half and is replaced by wheat population (50%). In general the most efi sien model in Kaliangkrik-Magelang was model III (50%), Samiran-Boyolali was model II (one side), and Kledung Temanggung were both model II (one side) and model III (50%) showed the highest value.
HYDROCARBON-DEGRADING POTENTIAL OF EXTREMELY HALOPHILIC ARCHAEA IN HYPERSALINE ENVIRONMENTS: A BRIEF REVIEW Yosmina H. Tapilatu
Widyariset Vol 15, No 2 (2012): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (855.316 KB) | DOI: 10.14203/widyariset.15.2.2012.375–384

Abstract

The potential of extremely halophilic archaea degrading hydrocarbon in hypersalin environment is reviewed. The emphasis is on the last 30 years studies. The identification of extremely halophilic archaea is important as this type of prokaryote is predominant in the hypersaline environment with NaCl concentration higher than 20% (w/v). Recent studies have shown that extremely halophilic archaea especially those belong to Halobacterium, Haloarcula, and Haloferax might play an important role in hydrocarbon degradation in hypersaline environment. Other that can be found in pristine and hydrocarbon-contaminated hypersaline environment, member of these genuses are also capable of using hydrocarbon as source of energy and carbon.